ライフサイエンスコーパス: glucanase

1 rified to homogeneity and shown to be a beta-glucanase.

2 nscription of genes encoding acidic beta-1,3-glucanase.

3 and turns the enzyme into an endo-beta(1,4)-glucanase.

4 ains a mutation in glgE, encoding a putative glucanase.

5 ivity to lysis after treatment with beta-1,3-glucanase.

6 e from a full-length cDNA clone for beta-1,3 glucanase.

7 elated PR-2d gene encodes an acidic beta-1,3-glucanase.

8 H16 family offers new insights into the GH16 glucanase.

9 eta1,3-glucanase and Eng1 is an endo-beta1,3-glucanase.

10 he larval gut lumen and is an active beta1,3-glucanase.

11 LicA had no homology with plant beta-glucanases.

12 superfamily, the Concanavalin A-like lectins/glucanases.

13 e predicted GAP include homologs of beta-1,3-glucanases (16), metallo- and aspartyl proteases (13), g

14 her defence-related genes (encoding 1,3-beta-glucanase, 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-Co

15 t containing a thermotolerant (1,3-1,4)-beta-glucanase (4.28 microg.g(-1) soluble protein) provides a

16 Transcripts encoding endochitinase, beta-1,3-glucanase, a thaumatin-like protein, ascorbate peroxidas

17 mino acid sequence determination of beta-1,3-glucanase-active proteins partially purified from cultur

18 ed endo-1,4-beta-mannanase and endo-1,4-beta-glucanase activities and hydrolyzed oligosaccharides wit

19 iochemically distinct extracellular beta-1,3-glucanase activities.

20 er endo-beta(1,4)-glucanase or beta(1,3;1,4)-glucanase activities.

21 Cel12A has strong endo-glucanase activity against xyloglucan and (1-->3,1-->4)-

22 sion or overexpression of endo-(1-->4)beta-D-glucanase activity has no detectable effect on fruit sof

23 on measurement of very weak endo-1,4-beta-d-glucanase activity in one family member.

24 account for nearly all of the total secreted glucanase activity of Histoplasma yeasts.

25 that engender predominant mixed-linkage endo-glucanase activity vis a vis predominant endo-xyloglucan

26 In culture, beta-1,6-glucanase activity was induced in the presence of beta-1

27 d gluC genes, encoding enzymes with beta-1,3-glucanase activity, were identified by a reverse-genetic

28 in the production of proteins with beta-1,3-glucanase activity.

29 milarity to several glucanases, but it lacks glucanase activity.

30 lus fumigatus sun proteins show a beta-(1,3)-glucanase activity.

31 s and any increase of alpha-amylase and beta-glucanase activity.

32 is a periplasmic protein with endo-beta-1,4-glucanase activity.

33 -mannanase activity and little endo-1,4-beta-glucanase activity; however, this enzyme also exhibited

34 These proteins include endo-1,3;1,4-beta-D-glucanase and alpha-L-arabinofuranosidase, which act on

35 beta-1,3-Glucanase and chitinase appeared in the micropylar tissu

36 tion induced the expression of both beta-1,3-glucanase and chitinase genes in naturally infected frui

37 Although both beta-1,3-glucanase and chitinase were expressed in tomato endospe

38 KOR1 is a membrane-anchored endo-beta1,4-glucanase and contains eight potential N-glycosylation s

39 trated that PcAce2 can restore the defective glucanase and endochitinase gene expression of the mutan

40 roteins and show that Exg8 is an exo-beta1,3-glucanase and Eng1 is an endo-beta1,3-glucanase.

41 rom an ancestral broad specificity GH16 beta-glucanase and evolved toward a bent active site topology

42 ion efficiency, however in combination, beta-glucanase and Flavourzyme(R) enhanced the extraction eff

43 beta-Glucanase and Flavourzyme(R) were used as the enzymes fo

44 enzyme concentration of 5% v/w each of beta-glucanase and Flavourzyme(R), temperature 50 degrees C a

45 IDFEFLG) that is also found in Bacillus beta-glucanase and may be important for enzyme activity.

46 ce related enzymes, i.e. chitinase, beta-1,3-glucanase and PAL, and higher content of epicatechin.

47 lysaccharides by digesting with endo-beta1,3-glucanase and with a novel endo-alpha1,3-glucanase (muta

48 We suggest that the pollen-coat beta-glucanase and xylanase hydrolyze the stigma wall for pol

49 During anther development, glucanase and xylanase transcripts appeared at a mid dev

50 t encode homologues to glycosyltransferases, glucanases and EPS transporters as well as regulatory pr

51 he presence of membrane-linked endo-beta-1,4-glucanases and it is suggested that these might also hav

52 PI proteins that are easily released by beta-glucanases and not attached to cell wall beta1,6-glucans

53 s, which are present in many fungi, regulate glucanases and other enzymes needed for cell wall remode

54 that Histoplasma yeasts secrete two beta1,3-glucanases and that Eng1 endoglucanase activity is the p

55 with endo- and exocellulase, xylanase, beta-glucanase, and acetyl xylan esterase activities.

56 ogenesis-related (PR) proteins PR1, beta-1,3-glucanase, and chitinase and also develop increased resi

57 -H as well as additional chitinase, beta-1,3-glucanase, and protease activities did inhibit the growt

58 blue-staining layer was digested by beta-1,3-glucanase, and these envelopes lost OD.

59 rged from a broad-specificity ancestral GH16 glucanase, and this inactive member of the GH16 family o

60 se, polygalacturonase beta-subunit, 1,3-beta-glucanase, and xyloglucan endotransglycosylase homologue

61 carboxymethyl)cellulases, mixed-linkage endo-glucanases, and endo-xyloglucanases.

62 shown to be part of the active site of other glucanases, and form a cluster that is distinct from pre

63 on these regions strongly suggests that the glucanases are a very ancient family of genes.

64 Similar glucanases are important in cellulose biosynthesis in ba

65 rved catalytic residue in GH16 endo-beta-1,3-glucanases, as essential for Rv0315 to induce immunologi

66 sed for activities of chitinase and beta-1,3-glucanase at pH 5 and 7, consistent with reduced express

67 PCR analysis, we identified an endo-beta-1,4-glucanase (AtCel5) of Arabidopsis thaliana that is expre

68 R genes (encoding endochitinase and beta-1,3-glucanase B) and at least one ACC oxidase gene, all of w

69 The Pd-associated beta-1,3-glucanase (BG_pap) and CALLOSE BINDING PROTEIN1 (PDCB1)

70 ion of an intracellular pool of the secreted glucanase Bgl2p, as well as to accumulation of Golgi-rel

71 encodes two enzymes, a surface endo-1,6-beta-glucanase, BT3312, and a periplasmic beta-glucosidase th

72 a region with sequence similarity to several glucanases, but it lacks glucanase activity.

73 Lycopersicon esculentum Mill.) endo-beta-1,4-glucanase Cel1 mRNA accumulation was previously correlat

74 It is a small (23-kD) endo-1,4-beta-glucanase (Cel12A) belonging to glycoside hydrolase fami

75 nsglycosylase (LeEXT1) and an endo-1, 4-beta-glucanase (Cel7), which, like LeExp2, are auxin-regulate

76 transformed with an antisense endo-1,4-beta-glucanase (cellulase, EC 3.2.1.4) Cel2 transgene under t

77 Higher gene regulation and beta-1,3-glucanase, chitinase activities were observed in cv. Rya

78 Also, enhanced activities of beta-1,3-glucanase, chitinase were noted in both cultivars.

79 The 1,3-beta-glucanase clones encoded a 37,645 Da protein with 57.6%

80 cillus halodurans is a multimodular beta-1,3-glucanase comprising an N-terminal family 81 glycoside h

81 escence analysis and treatment with beta-1,3 glucanase demonstrated that the expressed Msg fusion pro

82 phobicity phenotype were released by limited glucanase digestion.

83 thermostable cellulase, the endo-1,4-beta-D-glucanase E1 from Acidothermus cellulolyticus, was impor

84 beta-1,3-Glucanase (EC 3.2.1.39) and chitinase (EC 3.2.1.14) mRNA

85 CelA protein is a cellulase (endo-1, 4-beta-glucanase (EC 3.2.1.4)).

86 hat termite GNBP-2 (tGNBP-2) shows beta(1,3)-glucanase effector activity previously unknown in animal

87 7 and LeEXT, genes encoding an endo-1,4-beta-glucanase (EGase) and a xyloglucan endotransglycosylase

88 tion of the ripening-related endo-1,4-beta-D-glucanase (EGase) CaCel1 in fruit softening was investig

89 The ripening-related pepper endo-1,4-beta-D-glucanase (EGase) CaCel1 was over-expressed in transgeni

90 A cDNA (Cel1) encoding an endo-1,4-beta-glucanase (EGase) was isolated from ripe fruit of strawb

91 ta-galactosidase (beta-Gal), endo-1,4-beta-D-glucanase (EGase), and cellulase were monitored during g

92 A gene (EGL1) encoding an endo-beta-1,4-D-glucanase (EGase, EC 3.2.1.4) of pea (Pisum sativum) has

93 Plant endo-beta-1,3-glucanases (EGases) degrade the cell wall polysaccharide

94 Immunolocalization of endo-beta-1,4-glucanases (EGases) secreted from cyst nematodes was obs

95 Two cDNA clones encoding endo-beta-1,4-glucanases (EGases) were isolated from a radiata pine (P

96 activity and mRNA abundance of endo-1,4-beta-glucanases (EGases).

97 Endo-1,4-beta-D-glucanases (EGases, EC 3.2.1.4) are enzymes produced in

98 ural feature of many microbial endo-beta-1,4-glucanases (EGases, or cellulases) is a carbohydrate bin

99 The initial kinetics of endo-glucanases (EGs) is far less investigated, partly due to

100 Over-expression of an alfalfa glucanase-encoding gene confers significant protection a

101 on: the chitinase-encoding gene CTS1 and the glucanase-encoding gene SCW11.

102 Thirteen new beta-glucanase-encoding genes have been identified in the ric

103 a commercial preparation of Trichoderma endo-glucanase (EndoGase).

104 We have characterized endo-beta-1,3-glucanase (Eng) from 3 species of Pneumocystis.

105 beta-glucan polysaccharides by the secreted glucanase Eng1.

106 calizations of two potential Rho4 effectors--glucanases Eng1 and Agn1--are abnormal, and active Rho4

107 The addition of beta-glucanase enhanced the increase of ethyl octanoate, but

108 rom intact cells or cell wall fractions with glucanase enzymes but was retained in the cell wall afte

109 Histoplasma yeasts also secrete the putative glucanase Exg8, which may serve a similar role as Eng1 i

110 isera raised against the purified exo-beta-D-glucanase (ExGase) were used to select partial-length cD

111 elatively uncharacterized family of beta-1,3-glucanases, family GH-81.

112 the molecular cloning of the first beta-1,3 glucanase from animal tissue.

113 The antibody to A6 1,3-beta-glucanase from B. napus cross-reacted with a 56-kD prote

114 carbohydrate-binding domain in the 1,4-beta-glucanase from Cellulomonas fimi.

115 Da protein with 57.6% identity to a 1,3-beta-glucanase from soybean (Glycine max).

116 Next, recombinant endo-beta-1,6-glucanase from Trichoderma harzianum is utilized to rele

117 homology at the amino acid level to beta-1,3 glucanases from two species of bacteria and a clotting f

118 the impact of TO fumigation on the beta-1,3-glucanase gene expression was higher in both cultivars.

119 Phylogenetic analysis of the endo-beta-1,4-glucanase gene family of Arabidopsis and other plants re

120 Gns9 and a beta-glucanase gene from wheat were grouped in Subfamily D.

121 Like the A6 1,3-beta-glucanase gene products from Brassica napus and Arabidop

122 beta-1,3-Glucanase genes are clustered onto regions of five linka

123 dicated that at least 12 classes of beta-1,3-glucanase genes exist in the soybean.

124 ed sequences from coding regions of beta-1,3-glucanase genes from different species were used to ampl

125 probes for the different classes of beta-1,3-glucanase genes revealed that the mRNA levels of all cla

126 -O-methyltransferase (CHOMT), and 1,3-beta-D-glucanase genes was less rapid, with lag periods of 60 a

127 ated proteins, chitinases (CHT) and beta-1,3-glucanases (GLU), are stress proteins up-regulated as re

128 Class I chitinase (Chi9) and beta-1,3-glucanase (GluB) genes are expressed in the micropylar e

129 Some genes including glucanase, glutathione peroxidase, glutaredoxin, and a p

130 Growth-related 1,3;1,4-beta-glucanase Gns1 was classified in Subfamily B.

131 inal domain, which is similar in sequence to glucanases, had less affinity for the polysaccharides, d

132 Some beta-1,3-glucanases have already been structurally and biochemica

133 Both glucanases have near maximal activity at temperature and

134 hese genes, together with other monocot beta-glucanases, have now been classified into four subfamili

135 with the probe, one encoded a novel 1,3-beta-glucanase having a calculated molecular weight of 46.3 a

136 ry and characterization of hetero-trans-beta-glucanase (HTG), a transglycanase that targets cellulose

137 tified the central role of the endo-1,6-beta-glucanase in 1,6-beta-glucan depolymerization by deletin

138 enting mRNAs encoding chitinase and 1,3-beta-glucanase in cotton (Gossypium hirsutum L.) leaves.

139 copy and subcellular fractionation localized glucanase in ER-derived vesicles in the cytoplasm and th

140 Here, we report a novel role for beta-1, 3- glucanase in inducing Candida albicans to form filaments

141 r findings suggest that an inactive beta-1,3-glucanase in Mtb drives T-helper 1 (Th1) immune response

142 (KOR1) is an integral membrane endo-beta1,4-glucanase in the trans-Golgi network and plasma membrane

143 proteinaceous inhibitor of any endo-beta-1,4-glucanase, including the cellulases.

144 seB of soybean differ in susceptibility to a glucanase inhibitor protein (GIP1) produced by Phytophth

145 We show that soybean produces an apoplastic glucanase inhibitor protein, GmGIP1, that binds to PsXEG

146 racterization of a class of proteins, termed glucanase inhibitor proteins (GIPs), that are secreted b

147 The identity of this molecule as beta-1,3 glucanase is confirmed by sequence homology, by the pres

148 Sequence analysis indicates the beta-1,6-glucanase is homologous to enzymes secreted by the mycop

149 nation of chitinase and recombinant beta-1,3-glucanase is initially used, releasing all of the chitin

150 ndophyte Neotyphodium sp., a fungal beta-1,6-glucanase is secreted into the apoplast, and activity of

151 Glycosylation of the beta-glucanase isolated from the ceca testified to its origin

152 sis thaliana) membrane-bound endo-1,4-beta-d-glucanase KORRIGAN1 (KOR1) not only caused reduced CSC m

153 ved in innate immunity and the endo-beta-1,4-glucanase KORRIGAN1 required for cellulose biosynthesis.

154 38 nucleotides encoding LicA (1,3-1,4-beta-D-glucanase; lichenase) (EC 3.2.1.73) of 245 amino acids w

155 affinity interaction requires the C-terminal glucanase-like domain of betaGRP2.

156 LA domains of an insect modular protease and glucanase-like domains of a betaGRP mediate their intera

157 As a secreted protein, the beta-1,6-glucanase may have a nutritional role for the fungus.

158 ggest that the natural antifungal agent beta-glucanase may support morphologic transformation of Cand

159 Thus, these two endo-1,4-beta-D-glucanases may have a role in the sloughing of border ce

160 licylic acid, whereas the levels of 1,3-beta-glucanase mRNA are relatively unaffected.

161 Acidic beta-1,3-glucanase mRNA levels were initially similar in both roo

162 rthern analysis revealed that wheat 1,3-beta-glucanase mRNA was up-regulated in Al-intoxicated roots,

163 Accumulation of beta-1,3-glucanase mRNA, protein and enzyme activity was reduced

164 1,3-glucanase and with a novel endo-alpha1,3-glucanase (mutanase).

165 defense-related proteins such as chitinases, glucanases, myrosinases, and others showed enhanced secr

166 onstrate that Rv0315 is an inactive beta-1,3-glucanase of the glycoside hydrolase 16 (GH16) family.

167 s members that display either endo-beta(1,4)-glucanase or beta(1,3;1,4)-glucanase activities.

168 ls or their digestion products with beta(1-3)glucanase or beta(1-6)glucanase were carboxymethylated a

169 gi and no significant similarity to 1,3-beta-glucanases or glucosidases from other organisms.

170 The endo-beta-1,4-glucanases, or cellulases, of higher plants are cell wal

171 granule targets of the protease are beta-1,3 glucanase, ovoperoxidase, and the protease itself, but t

172 quence had high homology with bacterial beta-glucanases, particularly in the central regions and towa

173 modesmata Callose Binding 1 and the beta-1,3-glucanase PdBG2 and altered callose-mediated PD permeabi

174 Two plasmodesmal-localized beta-1,3 glucanases (PdBGs) were identified that regulate callose

175 Activities of secreted beta-1,3-glucanase, pectinase, and xylanase in culture filtrates

176 The beta-glucanase produced by E. coli was purified from the cult

177 More than 95% of the recombinant beta-glucanase produced in E. coli cells was found in the cul

178 gestion of yeast cell walls with beta(1-->3)-glucanase, reduction with borotritide, and subsequent in

179 LicA represented the first 1,3-1,4-beta-D-glucanase reported from fungi.

180 These endo-glucanases show a distinctive initial burst with a maxim

181 reparation containing predominantly beta-1,3 glucanase, substantially reduces the ability of this P.

182 efore and after treatment with a beta(1-->3)-glucanase, suggesting a specific interaction between the

183 eptide sequences were obtained from beta-1,3 glucanase that had been purified from eggs of the sea ur

184 in response to three family-12 endo-beta-1,4-glucanases that can specifically hydrolyze xyloglucan, c

185 its mRNA differ from those of the known beta-glucanases that hydrolyze the callose wall of the micros

186 requires the activity of an endo-1,4-beta-d-glucanase, the exact function of which in the synthesis

187 e expression of Basic Chitinase and beta-1,3-Glucanase, the GCC-box-containing genes.

188 hypothesis that, in analogy to Bacillus beta-glucanases, this region may be the active site of XET en

189 ta suggest that the contribution of beta-1,3-glucanases to the biocontrol activity of L. enzymogenes

190 in the anther tapetum of the plant, whereas glucanase transcript was distributed ubiquitously.

191 It was found that beta-glucanase treatment alone did not enhance the extraction

192 containing the gene for the (1, 3-1, 4)-beta-glucanase under the control of the Hor3-1 promoter.

193 ny organisms, including C. albicans, secrete glucanases under different environmental conditions.

194 in the sample treated with four fungal beta-glucanase units/g of bran.

195 enzymes alpha-amylase, beta-amylase and beta-glucanase varied by a factor of two to three.

196 DNA gel-blot analysis indicated the beta-1,6-glucanase was encoded by a single gene.

197 beta-1,3-Glucanase was expressed exclusively in the endosperm cap

198 This glucanase was not detected in hemolymph.

199 -RELATED genes, Basic Chitinase and beta-1,3-Glucanase, was upregulated in 35S:HDA19 plants but downr

200 nofuranosidases, 2 arabinanases and one beta-glucanase were assessed for their comparative enzymatic

201 products with beta(1-3)glucanase or beta(1-6)glucanase were carboxymethylated and fractionated on siz

202 From RNA gel blots, similar beta-1,6-glucanases were expressed in tall fescue (Festuca arundi

203 iosa (polygalactuoronase and endo-1,4- beta -glucanase) were infused into stems, and particle passage

204 ctions by PI-PLC and from cell walls by beta-glucanase, which implied that GFP was GPI-anchored to th

205 al attack is the production of endo-beta-1,3-glucanases, which are thought to play an important role

206 rinase is commonly used to describe beta-1,3-glucanases widespread throughout Archaea, bacteria, and

207 KOR1 encodes an endo-1,4-beta-d-glucanase with a transmembrane domain and two putative p

208 n-engineered thermotolerant (1, 3-1, 4)-beta-glucanase with the D hordein gene (Hor3-1) promoter duri

209 fida fusca xyloglucan-specific endo-beta-1,4-glucanase (Xeg)74 and the Xeg74 catalytic domain (CD) we

210 On maize pollen, three proteins, glucanase, xylanase, and a novel protease, Zea mays poll