ライフサイエンスコーパス: glutamate residue

1 ortant for the initial addition of the first glutamate residue.

2 caps the chain with a single alpha-linked l-glutamate residue.

3 ated version of the SH3 domain lacking a key glutamate residue.

4 -quinone cross-linked to the side chain of a glutamate residue.

5 obe the functional importance of a conserved glutamate residue.

6 was confirmed by mutagenesis of a conserved glutamate residue.

7 ion, where most myosins have an aspartate or glutamate residue.

8 interactions with the conserved active site glutamate residue.

9 han that of removal of a single gamma-linked glutamate residue.

10 ive to modifications in either metal-binding glutamate residue.

11 ase using this mechanism, is an aspartate or glutamate residue.

12 r is composed of six conserved histidine and glutamate residues.

13 terminal sequence mutation to add additional glutamate residues.

14 these roles frequently include aspartate and glutamate residues.

15 agenesis of a critical Lys(882) residue to a glutamate residue abolished all evidence of kinase activ

16 A glutamate residue adjacent to the box2 region in IFNLR1

17 lecular interaction between partially buried glutamate residues adjacent to the active site of Escher

18 es of the putative zipper were replaced with glutamate residues also failed to form stable rings.

19 C-tail clusters require Tha4's transmembrane glutamate residue and its amphipathic helix, both of whi

20 n, were mutated to alanine; in addition, the glutamate residue and one aspartate residue were mutated

21 The glutamate residue and the aromatic pteridine ring intera

22 om a neutral histidine residue to a negative glutamate residue and the disruption of the "dilysine tr

23 nts to a position that engages the catalytic glutamate residue and would block cognate protein substr

24 The mutants include the conversion of all 20 glutamate residues and 24 of the 25 aspartate residues i

25 cluster ligated by three histidine and four glutamate residues and activates dioxygen to perform its

26 Analysis of six conserved aspartate or glutamate residues and an additional 18 disease-causing

27 tions such as those caused by methylation of glutamate residues and by ligand-induced conformational

28 te-directed mutagenesis of nine aspartate or glutamate residues and four histidine residues to leucin

29 Cleavage occurs between cysteine and glutamate residues and results in a pyroglutamate produc

30 ormation: a gamma-glutamyl ligase adds 1-3 l-glutamate residues and the ATP-grasp-type ligase describ

31 Mouse CCP5 removes multiple glutamate residues and the branch point glutamate from t

32 lpolyglutamate synthetase (FPGS), which adds glutamate residues, and gamma-glutamyl hydrolase (GGH),

33 ids 24-48) that is enriched in aspartate and glutamate residues, and we previously speculated that th

34 wn, it is unknown whether single or multiple glutamate residues are added following each folate bindi

35 Glutamate residues are commonly found surrounding the me

36 ach active site, the catalytic histidine and glutamate residues are contributed by one monomer, and t

37 s, and two cytoplasmic amino-terminal domain glutamate residues are essential for this inhibitory act

38 the clan of GH-A enzymes, the two catalytic glutamate residues are located on strand 4 (the acid/bas

39 tallographic data suggested that neighboring glutamate residues associated with two of these tyrosine

40 We recently reported the importance of a glutamate residue at position 257 in the binding and tra

41 the E257D mutant protein also showed that a glutamate residue at position 257 of this permease is sp

42 the torsinA gene resulting in the loss of a glutamate residue at position 302 or 303 (torsinA DeltaE

43 Furthermore, potentiation required the glutamate residue at position 493.

44 l ATP sensitivity, while the mutation of the glutamate residue at position 51 to glycine (E51G) did n

45 electrostatic repulsions between the peptide glutamate residue at position 7 and 152D of the MHC mole

46 sine deaminase (ADA) contains an active site glutamate residue at position-217 that is highly conserv

47 fm-AAs) through the coupling reaction with a glutamate residue at the C-terminus and a glycine at the

48 eolytic cleavage site to acquire the optimal glutamate residue at the N-terminus.

49 ts of the substrate specificity, including a glutamate residue at the rim of the channel entrance tha

50 tion E262 shows that the naturally occurring glutamate residue at this position provides the optimal

51 domain elements and containing "activating" glutamate residues at loop and autophosphorylation sites

52 Glutamate residues at positions 9 and 13 and a basic ami

53 nels have a ring of eight negatively charged glutamate residues at the entrance to the intracellular

54 apore H3 domain modified by two gamma-linked glutamate residues at the epsilon-amino group of the COO

55 pends not only on the presence of a specific glutamate residue but also the presence of Cl(-) ions.

56 DRONC autoprocesses itself following a glutamate residue, but processes a second caspase, drICE

57 f variants with all possible combinations of glutamate residues changed to glutamine and arginine cha

58 son with prokaryotic CLC shows that a gating glutamate residue changes conformation and suggests a ba

59 nation of mutations that included a specific glutamate residue, conserved in all known Tim9 and Tim10

60 P(1) study identified that the corresponding glutamate residue, conserved in all S1P receptors, ion p

61 Mutant analysis showed that the glutamate residue corresponding to Asp(85) in bacteriorh

62 This glutamate residue corresponds to one of the residues cau

63 .5 MOPS buffer, suggesting that the original glutamate residue could be substituted with other ioniza

64 st evidence that the conserved aspartate and glutamate residues could be involved in the catalysis or

65 the oligomerization domain) was mutated to a glutamate residue, CPS formed an (alphabeta)2 dimer in t

66 ded phytochrome superfamily has identified a glutamate residue critical for bilin binding.

67 (AdoHcy) at 2.8 A resolution and identify a glutamate residue critical for its monomethylation behav

68 ciated with the deletion of one of a pair of glutamate residues (DeltaE302/303) near the C terminus o

69 However, the replacement of Thr(318) with a glutamate residue demonstrated that the simple addition

70 Loss of this single glutamate residue disrupts favorable electrostatic inter

71 However, the mutation of Tyr992 to a glutamate residue does not alter the receptor internaliz

72 Replacement of serines 152 and 153 with glutamate residues down-regulates the activity of NET1 a

73 V13'T in M2 together with neutralization of glutamate residues (E-1'A) and the adjacent insertion of

74 ribution due to a ring of negatively charged glutamate residues (E-2') at the narrow intracellular en

75 The role of two glutamate residues (E164 and E144) in the active site of

76 d at the bottom of this cleft is a conserved glutamate residue (E173) that is in position to play an

77 Site-directed mutagenesis of these two glutamate residues (E181A and E182A) along with another

78 Moreover, in RNase H, the glutamate residue E188 has been shown to be essential fo

79 Two glutamate residues, E199 and E408, align with the conser

80 nsport involves protonation of the conserved glutamate residue E373.

81 K+-binding site comprising four co-ordinated glutamate residues (E423 and E426 from each monomer) at

82 Three aspartate residues and a glutamate residue: E424, D498, D500 and D502, thought to

83 4938, D4945, D4953) with asparagine and four glutamate residues (E4942, E4948, E4952, E4955) with glu

84 ucrose synthase 3 (RSuS3), the two conserved glutamate residues (E678 and E686) in this motif and a p

85 Replacement of two unique glutamate residues, E9 and E13, from the cytoplasmic ami

86 Four glutamate residues (EEEE locus) are essential for ion se

87 a channels contain a cluster of four charged glutamate residues (EEEE locus), which seem essential fo

88 mental evidence that protonation of Glu37, a glutamate residue embedded in a hydrophobic pocket of Hd

89 In the Ca(2+)-binding site, two essential glutamate residues exhibit different conformations compa

90 ration, which appears to prime the catalytic glutamate residue for ATP hydrolysis.

91 The predicted catalytic glutamate residue for transglycosylase activity of bacte

92 on the top face of the Kelch domain and the glutamate residues form multiple hydrogen bonds with hig

93 The invariant catalytic glutamate residue found in lysozyme and various bacteria

94 Mutation of an invariant glutamate residue found within the catalytic domain of g

95 es; in particular, the conserved active site glutamate residues found in retaining glycosidases are p

96 S metal ion in a manner similar to that of a glutamate residue from collagen.

97 Removal of a glutamate residue from the binding site resulted in the

98 tive mutation in most cases is deletion of a glutamate residue from the carboxy terminal of torsinA,

99 The enzyme also removed multiple glutamate residues from side chains and C termini of pac

100 aGH) catalyze the hydrolysis of gamma-linked glutamate residues from the polyglutamyl of folates and

101 site-directed mutagenesis to include the two glutamate residues functioning in acid/base catalysis in

102 residues in Sso7d (D16 and D35) and a single glutamate residue (G54) showed significantly perturbed p

103 ng S2 cells, DIAP1 is cleaved by DRONC after glutamate residue Glu-205, located between the first and

104 a mechanism according to which the invariant glutamate residue (Glu-243) acts as a general base catal

105 rystal structure of T7 helicase shows that a glutamate residue (Glu-343), located at the subunit inte

106 docking studies have implicated a conserved glutamate residue (Glu-348) as a general base in the syn

107 Mutational analysis identified a titratable glutamate residue (Glu-536) in the linker between TM3 an

108 transient coordination to a highly conserved glutamate residue (Glu-67) during the catalytic cycle.

109 Mutagenesis of either of two conserved glutamate residues (Glu(66) and Glu(70)) within the Nudt

110 of betaS fibril structures indicate that key glutamate residues (Glu-31 and Glu-61) in these domains

111 vitro binding assays, we have identified two glutamate residues (Glu-849 and Glu-902) in beta(2)-adap

112 the role of the highly conserved C-terminal glutamate residue, Glu-590, on catalysis and malonyl-CoA

113 port the effects of mutations of active site glutamate residues, Glu(78) and Glu(122), on reactant bi

114 tions of two symmetry-related, lumen-exposed glutamate residues, Glu-122 and Glu-226.

115 e and that this caused the relocation of two glutamate residues (Glu181 and Glu182) into the proximit

116 diated by a single, evolutionarily conserved glutamate residue (Glu373) at a position where synapsin

117 As an alternative explanation, a specific glutamate residue (Glu59 in this flavodoxin), which is h

118 residues (His78, His161 and His225) and one glutamate residue (Glu77).

119 equence in Rad30, in which the aspartate and glutamate residues have each been changed to alanine.

120 is of a selection of conserved aspartate and glutamate residues, identified aspartate 145 as being es

121 Replacement of the external glutamate residue in domain II by cysteine reduces sodiu

122 ified that catalyze the epimerization of the glutamate residue in L-Ala-D/L-Glu.

123 dentified the amino-terminal neighbor of the glutamate residue in motif III as a determinant of pore

124 tributed to an additional negatively charged glutamate residue in PaNhaP.

125 In addition, we show the importance of this glutamate residue in sigma(54).DNA conformation sensing,

126 esidue at the end of the beta10 strand and a glutamate residue in the beta1-beta2 linker.

127 A conserved glutamate residue in the inhibitory helix motif was prev

128 ant role in histone substrate binding, and a glutamate residue in the protein core as playing an esse

129 We have now identified a highly conserved glutamate residue in the transmembrane region of E. coli

130 enzyme for collagen and the importance of a glutamate residue in the unique catalytic triad (Ser(278

131 cid with only one methyl group less than the glutamate residue in the wild-type enzyme, resulted in c

132 Mutations of this glutamate residue in Torpedo ray ClC channels alter gati

133 vidence of a H-bond between the ligand and a glutamate residue in transmembrane helix 3, which is con

134 The glutamate residue in turn might be further polarized thr

135 affects channel selectivity, suggesting that glutamate residues in both TM1 and TM3 face the lumen of

136 Exposed glutamate residues in CaM (Glu(11), Glu(14), Glu(84), an

137 Exposed glutamate residues in CaM (Glu-11, Glu-14, Glu-84, and G

138 GGCX), the enzyme responsible for converting glutamate residues in certain proteins to gamma-carboxyg

139 The enzyme has the expected two glutamate residues in close proximity to one another in

140 e show that a pair of invariant arginine and glutamate residues in each receptor alpha-subunit electr

141 ally mimicked for NCX3-B by mutating several glutamate residues in its cytoplasmic loop.

142 eriments show that aspargine, glutamine, and glutamate residues in MSP are perturbed by photooxidatio

143 We find that two consecutive glutamate residues in NOD2 are required for interaction

144 , 5 histidine, and 4 of the 28 aspartate and glutamate residues in the 202 residue VanX protein.

145 f methylation and demethylation of conserved glutamate residues in the Bacillus subtilis chemotaxis r

146 ent Cxs shows the presence of well conserved glutamate residues in the C terminus of TM-1; only Cx26

147 rected mutagenesis of conserved arginine and glutamate residues in the parasite-specific third cGMP s

148 ein and produces F420with predominantly 5-7l-glutamate residues in the poly-gamma-glutamate tail.

149 Four glutamate residues in the prototypic gamma-class carboni

150 By comparison, mutagenesis of aspartate and glutamate residues in the putative linker regions showed

151 functionally replaces one of the active site glutamate residues in the reactions catalyzed by crotona

152 catalyzed methylation (and demethylation) of glutamate residues in the signaling domains of methyl-ac

153 his study, we assessed the role of conserved glutamate residues in the Walker B domain of the two ATP

154 yotic Na(+)-channels is the presence of four glutamate residues in their selectivity filter.

155 e side-chain resonances of the aspartate and glutamate residues in uniformly (13)C-labeled alpha-lyti

156 s have confirmed that this unusual "bridging glutamate" residue in CntA is essential in catalysis and

157 likely filled by the analogous aspartate (or glutamate) residue in protein disulfide isomerase, DsbA,

158 backbone of rabbit CYP4B1; (ii) this I-helix glutamate residue influences substrate orientation in th

159 with this possibility, merely introducing a glutamate residue into the active site of a PTPS protein

160 n outside the cyclodextrin cavity, while the glutamate residue is anchored in the interior of the alp

161 d region includes a GIXE motif, in which the glutamate residue is completely conserved among the perm

162 ur interpretation is that this region of the glutamate residue is crucial in relaying the activating

163 al modification - polyglutamylation, where a glutamate residue is enzymatically linked to the gamma-c

164 A glutamate residue is identified as the key contact with

165 activity, respectively, indicating that this glutamate residue is important but not critical to catal

166 A conserved essential glutamate residue is involved in this interaction.

167 of an E809A mutant showed that the conserved glutamate residue is not relevant for the PatZ catalytic

168 However, a glutamate residue is not the only residue capable of fun

169 rom Escherichia coli indicate an unspecified glutamate residue is phosphorylated.

170 We show that a single anionic glutamate residue is responsible for maintaining the dis

171 de that the steric hindrance provided by the glutamate residue is responsible for the heme orientatio

172 The water molecule, which bridges two glutamate residues, is reminiscent of the enolase active

173 nt protein shows that the negatively charged glutamate residue lies on the surface of the N-terminal

174 However, mutation at two consecutive glutamate residues located within the hinge between the

175 Glutamine substitutions in two conserved glutamate residues, located within predicted transmembra

176 erefore, the protonation of aspartate and/or glutamate residues must provide an additional driving fo

177 ene, which changes the charge at a conserved glutamate residue near the outer mouth of the pore, has

178 ing and selectivity, a set of four conserved glutamate residues near the external mouth of the pore.

179 These roles include polarization by a glutamate residue of a water molecule for the attack on

180 We found that a glutamate residue of GIRK2 (E315), located on a hydropho

181 mutagenesis, we demonstrated that the first glutamate residue of the conserved DEIDFEFL motif (E97)

182 The glutamate residue of the HXE motif is catalytically requ

183 7 transgenes were mutagenized to replace the glutamate residue of the HXE motif, and transgenic plant

184 the posttranslational modification of select glutamate residues of its vitamin K-dependent substrates

185 the mannitol permease and the corresponding glutamate residues of other PTS permeases play important

186 out the spatial arrangement of two conserved glutamate residues of rat liver enoyl-CoA hydratase to w

187 In vertebrates, the modification of glutamate residues of target proteins is facilitated by

188 e of Glu181, the second of the two invariant glutamate residues of the double E loop that coordinate

189 lpha-helix-turn-beta-strand motifs, with the glutamate residues of the EEEE motif located in the turn

190 Furthermore, we show that a glutamate residue on E3 is involved in catalyzing ubiqui

191 from its catalytic histidine to aspartate or glutamate residues on 43-kDa membrane proteins.

192 A ring of glutamate residues on its extracellular side forms the s

193 Along with neutralization of glutamate residues on the B loop, these changes cause a

194 in ligation with the burial of three charged glutamate residues on the same helix.

195 The high density of aspartate and glutamate residues on the surface follow a helical patte

196 , we measured acid dissociation constants of glutamate residues on troponin C and of histidine on ske

197 These results indicate that both glutamate residues play crucial roles in the hydration c

198 2 form extensive hydrogen-bond networks with glutamate residues predominantly in the unstructured, ac

199 3 into the FtsY active site orients a single glutamate residue provided by Ffh (E277), triggering GTP

200 A close approach of two key glutamate residues provides an elegant mechanism for the

201 Substitution of arginine 282 with a glutamate residue (R282E-PepT1) gave a protein at the pl

202 ltetrahydropterin synthase (PTPS) in which a glutamate residue replaces or accompanies the catalytic

203 eaction in the absence of a highly conserved glutamate residue seen in Gcn5-related N-acetyltransfera

204 mutant in which histidine was replaced by a glutamate residue showed preferential heterodimer format

205 S of an altered conformation, in which a key glutamate residue swings out toward one of the heme prop

206 The active site contains a glutamate residue that contacts the substrate, but, beca

207 The E166A mutant lacks a critical glutamate residue that has a key role in the deacylation

208 Each subunit contains a buried glutamate residue that is essential for function, and th

209 ime identified and characterized a catalytic glutamate residue that is involved in the energy transdu

210 is known only inferentially by two conserved glutamate residues that act as way-stations for H(+) in

211 MH1 and MH2 contain glutamine and glutamate residues that are subject to deamidation, meth

212 ficance of strategically placed arginine and glutamate residues that form interacting pairs in respon

213 tituting each of the four negatively charged glutamate residues that form the ion selectivity filter

214 e occurrence of carboxylate shifts involving glutamate residues that ligate the catalytic iron atoms.

215 res are characterised by conserved aspartate/glutamate residues that provide ligands for metal bindin

216 nsity of acidic residues, including specific glutamate residues that serve as receptor adaptation sit

217 Ca channels rely on four glutamate residues (the EEEE locus), whose carboxyl side

218 E144A, we studied the effect of shifting the glutamate residue to all sites within helix V and three

219 We show that the enzyme adds a glutamate residue to both F(420)-0 and F(420)-1 in two d

220 In contrast, modification of either of the glutamate residues to alanine caused a dramatic decrease

221 he processive addition of approximately four glutamate residues to DDAH 4PteGlu 1.

222 atalyses the GTP-dependent addition of two L-glutamate residues to F(420)-0 to form F(420)-2.

223 ynthesis pathway by successive additions ofl-glutamate residues to F420-0, producing a poly-gamma-glu

224 nstrate that full-length FbiB adds multiplel-glutamate residues to F420-0in vitroto produce F420-5 af

225 -translational conversion of certain protein glutamate residues to gamma-carboxyglutamate.

226 ational modification, gamma-carboxylation of glutamate residues to Gla.

227 with the known ability of negatively charged glutamate residues to substitute functionally, in some c

228 FB, an enzyme that catalyzes the addition of glutamate residues to the folate molecule to form folylp

229 red", electrostatic networks of arginine and glutamate residues to the surfaces of the coiled-coil bu

230 ical disorder caused by deletion of a single glutamate residue (torsinA DeltaE) in the C-terminal reg

231 licated in substrate binding were changed to glutamate residues using PCR based site-directed mutagen

232 not completely abolished when the invariant glutamate residue was altered.

233 It was found that protonation of a single glutamate residue was enough to modify the conformation

234 t and for both mu and delta receptors when a glutamate residue was incorporated into the extracellula

235 Glutamate residues were also introduced mutagenically as

236 residue (F680) that resides between the two glutamate residues were changed by site-directed mutagen

237 1p and Phr2p was conducted and two conserved glutamate residues were shown to be essential for activi

238 Hence, aspartate and glutamate residues were systematically substituted with

239 31 novel lipophilic TS inhibitors, lacking a glutamate residue, were synthesized; 26 of them had in c

240 s the charge-charge repulsion from the three glutamate residues when carbamate passes through the tun

241 -CRD interface, is coordinated by a triad of glutamate residues which are, to some extent, neutralise

242 ring aspartate substitutions for each of the glutamate residues which normally undergo gamma-carboxyl

243 een the positively charged drug and a buried glutamate residue, which is the key to cation selectivit

244 SASP-C of Bacillus megaterium two conserved glutamate residues, which form part of the germination p

245 The presence of one glutamate residue with its chain directed toward the int

246 ring penetrates into the CD cavity while the glutamate residue with the aminobenzoic part of FA is ex

247 inability of K(+) to fit between a plane of glutamate residues with the preferred solvation geometry

248 We further identify a glutamate residue within a conserved region adjacent to

249 in EmrE is defined by two negatively charged glutamate residues within a hydrophobic pocket formed fr

250 The ion selectivity locus comprises four glutamate residues within the channel's pore.

251 involves reversible methylation of specific glutamate residues within the cytoplasmic domains of met

252 modified by carboxyl methylation of specific glutamate residues within their cytoplasmic domains.