ライフサイエンスコーパス: glycan

1 due to the destabilizing effect of a bulky N-glycan.

2 nd composition of the Fc-associated N-linked glycan.

3 n of EREC was dependent on cellular N-linked glycans.

4 e a conserved motif buried under a canopy of glycans.

5 oproteins contain highly abundant sulfated N-glycans.

6 the released, deutero-reduced permethylated glycans.

7 rides into structurally uniform human-type N-glycans.

8 sample preparation for studying the N-linked glycans.

9 regard to tri-antennary and tetra-antennary glycans.

10 and blood group antigens present on N- and O-glycans.

11 ries only mammalian-atypical oligomannosidic glycans.

12 are required to depolymerize highly complex glycans.

13 uropathy, has abundant GlcNAc-6-O-sulfated N-glycans.

14 cetylglucosamine branching of Asn (N)-linked glycans.

15 ion of E-cadherin was dependent on the O-Man glycans.

16 ted effects on the structure of cell-surface glycans.

17 low-abundance, trace and even ultra-trace N-glycans.

18 ents in cells, especially for highly complex glycans.

19 upied by high-mannose/hybrid or complex-type glycans.

20 cell wall polysaccharides and other complex glycans.

21 cal basis for the stabilizing effects of the glycans.

22 observable in fragments derived from larger glycans.

23 fide bond, and density corresponding to 22 N-glycans.

24 acid and is predominantly expressed on VWF O-glycans.

25 olysaccharide but targets a fungal cell wall glycan, 1,6-beta-glucan, which is a growth substrate for

26 We found that the tri-antennary glycan [A3(2,4,2) type] terminated with N-acetylglucosam

27 lytically inactive Glt1, indicating that the glycan acts in concert with the first enzyme in the path

28 On the other hand, the IgE-binding glycan allergen galactose-alpha-(1,3)-galactose (alpha-G

29 ontext, a mature bi-antennary complex-type N-glycan also could be core-fucosylated by FUT8, albeit at

30 s spectrometry has become a primary tool for glycan analysis thanks to its speed and sensitivity, but

31 combinant CLEC3A by SDS-PAGE and immunoblot, glycan analysis, matrix-assisted laser desorption ioniza

32 Despite recent technological advances in glycan analysis, structural elucidation of WTAs remains

33 a glycosylation profile, albeit mainly at a glycan and glycopeptide level of analysis.

34 To our knowledge, this novel glycan and its unique modification sites have not been r

35 rference of HCA1 interaction with both the N-glycan and peptide-moiety of SV2.

36 zing radiation increases high mannose-type N-glycans and decreases glycosaminoglycans.

37 (C) was fully processed with mature N-linked glycans and did not require the GPI anchor for localizat

38 GlcNAc6ST-1) failed to synthesize sulfated N-glycans and exhibited abnormal myelination and axonal de

39 In this review the effect of glycans and glycan-specific IgE on sensitization to alle

40 ocarbohydrate epitopes present on rabbit IgG glycans and lacking in humans.

41 N-glycans and leg domains in each subunit that connect the

42 GIG enables quantitative analysis of N-glycans and O-glycans from a single specimen and can be

43 to identify and quantify low-abundance IgG N-glycans and show some of these IgGs can be used as bioma

44 microbial interactions with endogenous host glycans and the importance of microbial polysaccharides.

45 The synthesis of glycans and the sorting of proteins are critical functio

46 were attributed to hyposialylation of the Fc glycan, and IgG from T2DM patients was also hyposialylat

47 as dependent on intact disulfide bonds and N-glycans, and only two antibodies recognized native Sigle

48 GT1), which catalyzes monoglucosylation of N-glycans, and oppositely acting glucosidase II (GlucII),

49 the structures of rare arabinosyl-containing glycans, and potentially for facilitating their modifica

50 When a V3 glycan- and E64K-modified trimer variant, BG505 SOSIP.66

51 aminoglycan modification and for an "ancient glycans" approach to dating of Neu5Gc loss during the ev

52 glycosylation site, and their occupancy by N-glycans are all detected and identified in a single expe

53 Oligomannose-type glycans are among the major targets on the gp120 compone

54 e (ABC) transporter-dependent pathway, where glycans are completed on undecaprenyl diphosphate carrie

55 l, native heterogeneously glycosylated IgG N-glycans are first deglycosylated with a wild-type endogl

56 Relatively low levels of N-glycans are found on transmembrane mucins, and their str

57 The synthetic glycans are found to have adjuvant activities in vivo.

58 Here, we report that sulfated N-glycans are involved in peripheral nervous system (PNS)

59 Glycans are major nutrients available to the human gut m

60 s can be released by glycosidases, whereas O-glycans are often cleaved by chemical reaction.

61 eins involved in the degradation of specific glycans are organized into co-regulated polysaccharide u

62 How these glycans are recognized and how they alter the course of

63 igh preference for avian receptor analogs by glycan array analyses.

64 Glycan array analysis confirmed selective binding of the

65 Glycan array analysis revealed a preference for alpha2,3

66 Screening of a glycan array demonstrated GalNAcbeta1-4(Fucalpha1-3)GlcN

67 ate and similar substrate specificities on a glycan array.

68 By applying the syntax to the analysis of glycan-array data, we found that the lectin AAL had high

69 These findings offer insight into glycan assembly mechanisms and the generation of antigen

70 id and I-EGF3 interface, or the beta3-N452 N-glycan at the I-EGF1 domain rendered beta3 integrin more

71 Glycans at Asn-168 were predominantly sialylated with bi

72 superfamily carries O-linked mannose (O-Man) glycans at highly conserved residues in specific extrace

73 ion were enriched in ApoC-III, di-sialylated glycans at multiple A1AT glycosylation sites and desialy

74 he critical influence of the unique N-linked glycan attached at Asn-297 on the structure and function

75 that intra-domain interactions involving the glycan attached to residue GluN1-N440 stabilize closed-c

76 era collected between 1979 and 2010 to block glycan binding of four pandemic GII.4 noroviruses isolat

77 A large glycan binding pocket extends into the dimeric interface

78 cans possess significant chemical diversity; glycan binding proteins (GBPs) recognize specific glycan

79 nctional ties, we could maintain ThreeFoil's glycan-binding capacity.

80 Probing the array with several glycan-binding proteins uncovered that not only terminal

81 The PUL also includes two glycan-binding proteins, confirmed by beta-mannan affini

82 y hampers investigations into the biology of glycan-binding proteins, which in turn complicates the b

83 Glycan-binding proteins, which include galectins, are in

84 develop new therapeutic interventions using glycan-binding proteins.

85 The glycan-binding site of T1 sigma1 is located in the head

86 potential drug by which modulation of neural glycan biosynthesis and thus function can be achieved in

87 on induces a cascade of events which impacts glycan biosynthesis through Hexosamine Biosynthesis Path

88 co-operatively limit metabolite supply to N-glycan biosynthesis, an activity with widespread implica

89 endently and additively to increase N-linked glycan branching.

90 also bound to the unmutated ancestor of a V2-glycan broadly neutralizing Ab, but this Ab type was not

91 instead of allowing otherwise subdominant V2-glycan broadly neutralizing Abs to develop.

92 ans by PNGase F digestion; (iv) release of O-glycans by beta-elimination using ammonia in the presenc

93 by carbodiimide coupling; (iii) release of N-glycans by PNGase F digestion; (iv) release of O-glycans

94 Quantitative analysis of glycans can be achieved by labeling their reducing end u

95 N-glycans can be released by glycosidases, whereas O-glyca

96 coepitopes but also complex architectures of glycans can influence binding selectivity in unanticipat

97 It is formed by linear glycan chains composed of N-acetylmuramic acid-(beta-1,4

98 hydrate derivatives and more than 50% of PDB glycan chains have at least one carbohydrate derivative

99 rst time, variations in miscanthus cell wall glycan components were comprehensively characterized acr

100 enables the simultaneous determination of N-glycan composition and N-glycosites with a deeper covera

101 Aubergine glycans contain abundant heteroxylan epitopes, some of w

102 is work suggests that O-fucose and O-glucose glycans cooperatively stabilize individual EGF repeats t

103 However, Man5GlcNAc2 glycan could be efficiently core-fucosylated by FUT8 in

104 Asymmetric bi- and triantennary glycans could be obtained by removal of a terminal beta-

105 grading enzymes are optimized to protect the glycan cues that activate the specific PULs ensuring a c

106 Glycan-deficient Env derivatives can be used as priming

107 /LesA, VirK, and four families involved in N-glycan degradation, NixE, NixF, NixL, and FucA1.

108 The Bacteroides are generalist glycan degraders, and this function is mediated largely

109 revealing increased sialyation on urinary N-glycans derived from prostate cancer patients.

110 four fucose residues were detected on its N-glycans, despite these lacking the fucosylated antennae

111 ell and T cell recognition to a single short glycan displayed at the surface of a virus-like particle

112 These O-Man glycans do not appear to be elongated like O-Man glycans

113 gens in rabbits, and found that the blocking glycans eliminated the induction of tier-1 NAbs to V3-ep

114 eversibly attached to receptors by metabolic glycan engineering.

115 ential, structural characterization of these glycan epitopes remains a significant challenge in mAb p

116 r release of the more highly core-modified N-glycans, especially those occurring in C. elegans, but n

117 tissue, which represents the most complex N-glycan ever synthesized.

118 ble to human colonic Bacteroides species are glycans, exemplified by pectins, a network of covalently

119 N- and O-linked glycans express structural elements of HMOs, and thus, t

120 Quantifying the glycan expression status on cell surfaces is of vital im

121 Os can be a useful model to study intestinal glycan expression, huNoV-intestine interaction, and huNo

122 developed a glycoprotei n immobilization for glycan extraction (GIG) method that allows for the simul

123 The structure reveals that the glycan fills up a surface groove of the EGF with multipl

124 model for BG1 recognition of V1V2 involving glycan flexibility.

125 g infection, with many showing dependence on glycans for binding to Env.

126 involved in nucleotide-sugar production and glycan formation, but the functional characterization of

127 eparation of an asymmetric tetra-antennary N-glycan found in human breast carcinoma tissue, which rep

128 e enzymes and the full identification of the glycans found in viral fibers remain incomplete.

129 ans do not appear to be elongated like O-Man glycans found on alpha-dystroglycan (alpha-DG), and we r

130 nd illustrate a general method for designing glycan-free folded protein derivatives.

131 bi-, tri-, and tetra-antennary asymmetric N-glycan from a single precursor.

132 e then applied this methodology to profile N-glycans from 58 prostate cancer patient urine samples, r

133 les quantitative analysis of N-glycans and O-glycans from a single specimen and can be applied to a h

134 nd A) or anhydrous hydrazine to cleave the N-glycans from glycopeptides.

135 After removing N-glycans from the original sample aliquot, O-glycans were

136 N-glycans fulfill multiple structural and biological funct

137 is of vital importance for insight into the glycan function in biological processes and related dise

138 zation of novel GBPs and characterization of glycan-GBP interactions are significant to provide poten

139 UT8, but the A3(2,2,6) type of tri-antennary glycan, generated by GnT-V, is not a substrate for FUT8.

140 Removal of the core fucose of this glycan greatly increases the affinity for FcgammaRIII, r

141 However, linker O-glycans greatly impact cellulose conversion via their co

142 The glycan group of the anti-PSMA antibody D2B was chemoenzy

143 ll documented; however, whether the N-linked glycan has a similarly critical role in multimeric, avid

144 te specificity of FUT8 toward bi-antennary N-glycans has been reported, but it is unclear with regard

145 The assignment of a direct role for GIPC glycan head groups in the impaired processes in iput1 mu

146 for site specific quantification of N-linked glycan heterogeneity present on an IgG1 mAb molecule con

147 vestigations regarding the relevance of anti-glycan IgE for allergic diseases.

148 d glycoforms, suggesting a role of IgG1 Fc N-glycan in optimizing the interface with the Fc receptor

149 d enzymes to metabolize the most challenging glycan in the human diet.

150 8) to directly fucosylate full-size mature N-glycans in a chemoenzymatic approach.

151 ave compared the recognition of mannose type glycans in melanocytes (HEMa-LP) and melanoma cells orig

152 ir detailed structure, the function of these glycans in planta, and the mechanisms by which they are

153 Synthesis of homogenous glycans in quantitative yields represents a major bottle

154 but also to high-mannose and complex-type N-glycans in the context of N-glycopeptides, N-glycoprotei

155 , and GnTIV) involved in early remodeling of glycans in the mammalian glycosylation pathway.

156 CD4-binding site (CD4BS), glycans in the V1/V2 and V3 regions and membrane proxima

157 odulated by the presence/absence of the N332 glycan, indicating an overlap between the two epitopes.

158 How these glycans influence brain function is only now beginning t

159 Thus, targeting Gal-1-glycan interactions may contribute to reinforce antibact

160 docking of LDNF identified key residues for glycan interactions.

161 agreed with the NMR data indicated that the glycan interacts with the loop connecting two alpha-heli

162 deconstruct algal or plant polysaccharides (glycans) into monosaccharides.

163 of a GlcNAc on the alpha1,3 mannose arm of N-glycan is essential for FUT8-catalyzed core fucosylation

164 Similarly, the procurement of defined glycans is key to establishing structure-activity relati

165 is well suited to identify and characterize glycan isomers directly, which presents tremendous chall

166 of waitlisted renal transplant patients to 3 glycan knockout (KO) pig cells and class I swine leukocy

167 N-glycan knockouts on the CD demonstrate that N-glycosylat

168 taomicron uses the most structurally complex glycan known: the plant pectic polysaccharide rhamnogala

169 Sequential glycan KO modifications significantly reduce antibody bi

170 hat FUT8 can catalyze core fucosylation of N-glycans lacking an alpha1,3-arm GlcNAc in cells.

171 vitro FUT8-catalyzed core fucosylation of N-glycans lacking the alpha1,3-arm GlcNAc moiety.

172 identified by searching against a predefined glycan library owing to unanticipated modifications.

173 After cytokine priming, Siglec-8 mAb or glycan ligand binding causes eosinophil apoptosis associ

174 Selectin interactions with fucosylated glycan ligands mediate leukocyte rolling in the vasculat

175 ng, specificities for sialic acid-containing glycan ligands, any cell could, in principle, invoke thi

176 Resolution of the anomeric alpha/beta glycan linkage within oligosaccharides remains a particu

177 model of Ebola virus disease and that GP1 N-glycan loss does not influence immunogenicity or vaccina

178 s work could be the basis for advancing anti-glycan mAb characterization.

179 A significant challenge of traditional glycan mapping techniques is that they do not provide si

180 tive pressure by ssNAbs to maintain the N332 glycan may have constrained the bNAb escape pathway.

181 Heterogeneous glycans may be bound to different amino acid residues, f

182 of molecular tools for glycoscience, such as glycan microarrays, affinity resins, and reference stand

183 results offer an unprecedented view of how a glycan modification influences a disordered region of a

184 Polysialic acid is a glycan modification of the neural cell adhesion molecule

185 g protein-specific adaptations to particular glycan modifications, yet always cleaved the peptide bon

186 ation to provide protein sequence-specific N-glycan modifications.

187 This novel glycan modifies the flagellin proteins (FlaBs) of T. den

188 utilized the unique structural features of N-glycan molecules, the common core sugar sequence (HexNAc

189 n interactions on arrays of simple synthetic glycans, more complex natural glycosaminoglycans (GAG),

190 built an algorithm to predict the types of N-glycan most likely to predominate at all the putative N-

191 lgorithm for the analysis and description of glycan motifs with high complexity.

192 ions predict an additional role for linker O-glycans, namely that they are responsible for maintainin

193 In contrast, removal of the beta3-N371 N-glycan near the beta3 hybrid and I-EGF3 interface, or th

194 ycoproteins and observed an enhancement in N-glycan occupancy that was further supported by modeling

195 The identified glycan of mass 450.2 Da is composed of a monoacetylated

196 ctly identified on the polylactosamines of N-glycans of SKOV3, IGROV1, OV90, and OVCA433.

197 ct of specific combinations of sugars in the glycan on ADCC remains to be further addressed, however.

198 The glycan on GluN2B-N688 shows a similar, though weaker, ef

199 ws for the simultaneous analysis of N- and O-glycans on a solid support.

200 alpha-dystroglycan, and moreover, the O-Man glycans on cadherins are not elongated.

201 eltaG partially or fully denuded of N-linked glycans on GP1 protected mice against ma-EBOV challenge,

202 ggest an intramolecular potentiating role of glycans on NMDA receptors.

203 Glycans on proteins not only mediate a variety of protei

204 gars for the formation of cereose-containing glycans on spores, whereas others such as Bacillus anthr

205 O-glycans on the CBM have little impact on binding, proteo

206 bulky residues, incorporated the modeling of glycans on the surface of gp120, and utilized continuum

207 PI-modification signal, a highly conserved N-glycan or the deletion of predicted O-glycosylation site

208 Binding of sialylated glycans or other ligands triggers signals that inhibit o

209 The use of N-glycan oxazolines, high energy intermediates on the hydr

210 Glycans partially shield Env from recognition by the hos

211 Remarkably, the glycan patterns, glycosylation site, and their occupancy

212 in glycosylation enzymes; both root hair and glycan phenotypes were restored upon reintroduction of A

213 Glycans possess significant chemical diversity; glycan b

214 It was found that removal of N-glycans prior to enrichment of O-linked glycopeptides by

215 ymes GlucII and UGGT1, which are involved in glycan processing and nascent protein folding.

216 Notably, N-linked glycans produced by GNE-deficient cells displayed enhanc

217 which glycosylation sites contain conserved glycan profiles across many trimeric Envs.

218 Glycan Reader has been improved and now identifies most

219 CHARMM-GUI Glycan Reader is updated to generate the simulation syst

220 not be correctly recognized by the original Glycan Reader.

221 Typhoid toxin binding to its glycan receptor Neu5Ac is central, but, due to the ubiqu

222 alpha2,3-sialylated and alpha1,3-fucosylated glycan receptors, including 6-sulfo-sialyl Lewis x (6-su

223 shown to be required for binding of multiple glycan receptors.

224 212) increased the binding affinity to those glycans recognized by the HA190 mutant.

225 Sonication achieves O-glycan release in 1 h.

226 obilization, sialic acid modification, and N-glycan release.

227 rresponding deglycosylated peptides, and the glycans released by the endoglycosidase PNGase F.

228 ematical optimization and evaluation using N-glycans released from several glycoprotein standards and

229 etailed molecular map of a trimer-associated glycan remodeling that forms a localized subdomain of th

230 llular glycosyltransferase, able to exert a "glycan remodelling" process, even at distal compartments

231 peptidases showed selectivity for different glycans, revealing protein-specific adaptations to parti

232 implify glycoprotein analysis by integrating glycan sequencing and glycopeptide analysis in a single

233 we combine comprehensive mass spectrometric glycan sequencing and molecular dynamics simulations to

234 Due to a lack of high throughput glycan sequencing software, glycan spectra are predomina

235 erstand the conformational properties of the glycan shield covering the surface of the HIV gp120/gp41

236 p120/gp41 envelope (Env) trimer, and how the glycan shield impacts the accessibility of the underlyin

237 Further, we found that removal of the heavy glycan shield surrounding conserved regions of the glyco

238 We found a lack of glycan sialyation on EGFR-K521 that associated with redu

239 the structural consequences of individual N-glycan site on integrin activation remain unclear.

240 ed the precise structural location of each N-glycan site, but the structural consequences of individu

241 roximal external region (MPER), and the V1V2 glycan site.

242 us maps for Cel7A glycosylation that include glycan sites and motifs.

243 s, we dissected the function of individual N-glycan sites in beta3 integrin activation.

244 exchange in the C-terminal region near the N-glycan sites, suggesting this region had become more ord

245 es and/or enable reglucosylation of N-linked glycans situated at variable distances from the site of

246 multiple glycosylation sites, the individual glycan species present at a particular site cannot be di

247 Glycan-specific IgE antibodies cross-react with highly s

248 In this review the effect of glycans and glycan-specific IgE on sensitization to allergens and al

249 lpha-Gal is immunogenic in humans and causes glycan-specific IgG and also IgE responses with clinical

250 These findings illustrate how glycan-specific maturation enables a human Ab to cope wi

251 alizing scFv monoclonals with CD4bs and N332 glycan specificities from India.

252 high throughput glycan sequencing software, glycan spectra are predominantly sequenced manually.

253 We show that purified PBP2a can cross-link glycan strands bearing penta- and triglycine, but not mo

254 sents a mass spectrometry-based approach for glycan structural analysis with immense potential.

255 the glycopeptides with different classes of glycan structural variants, including high mannose, sial

256 y, but the information content regarding the glycan structure of protonated glycoconjugates is hinder

257 addition, the Glycoforest algorithm detected glycan structures from MS/MS spectra missing a manual an

258 he production of abnormal truncated N-linked glycan structures instead of the typical bisected forms.

259 atalyzes the first step toward other various glycan structures present on alpha-dystroglycan of unkno

260 also offers a new functionality to edit the glycan structures through addition/deletion/modification

261 E-deficient cells produced distinct N-linked glycan structures with increased branching and extended

262 s 5-8 d to prepare the natural or modified N-glycan substrates, 3-4 d to engineer the IgG N-glycosyla

263 Because of the high diversity of glycan substrates, the functions of these enzymes are of

264 tions also alter binding to minor human-type glycans, suggesting that host adaptation may contribute

265 rt the idea that antibodies targeting the V3 glycan supersite might be useful for the treatment and p

266 Monoclonal antibody 10-1074 targets the V3 glycan supersite on the HIV-1 envelope (Env) protein.

267 ing approach for mAb production against some glycan targets.

268 nated in N-acetylneuraminic acid, as well as glycans terminated in N-glycolylneuraminic acid (Neu5Gc)

269 Typhimurium, we found that ArtB binds human glycans, terminated in N-acetylneuraminic acid, as well

270 the chemical synthesis of a tetra-antennary glycan that has N-acetylglucosamine (GlcNAc), N-acetylla

271 depends on their ability to degrade dietary glycans that cannot be metabolized by the host.

272 have evolved to recognize the dense array of glycans that coat the surface of the viral molecule.

273 is confirmed selective binding of the CRD to glycans that contain Manalpha1-2Man epitopes.

274 oxyTMT) enable multiplexed quantification of glycans through comparison of reporter ion intensities.

275 We used glycan TMT-labeling to improve electrophoretic migration

276 n binding proteins (GBPs) recognize specific glycans to translate their structures to functions in va

277 igosaccharides can be used as models for the glycans, to study processes such as cell wall biosynthes

278 Despite mammalian glycans typically having highly complex asymmetrical mul

279 se, pectin, and arabinogalactans, as well as glycans unique to algae.

280 ized in terms of sialic acid linkage of each glycan using the solid-phase esterification/amidation st

281 and (vi) data analysis for identification of glycans using in-house developed software.

282 lone (PMP) to prevent alditol peeling from O-glycans; (v) mass spectrometry (MS) analysis; and (vi) d

283 ZIKVs lacking the glycan were highly attenuated for the ability to cause m

284 -glycans from the original sample aliquot, O-glycans were chemically released from urinary exosomes a

285 cimannosidic, high-mannose, and complex type glycans were identified and their relative abundances we

286 Major sulfated N-glycans were identified in both porcine and mouse PNS my

287 C1INH sialylated-N- and -O-glycans were not only essential for its interaction with

288 We found that sialic acid-bearing glycans were recognized by germline-encoded and somatica

289 Some detailed structures of these glycans were revealed through liquid chromatography/tand

290 Both added glycans were shown to be predominantly of the Man6GlcNAc

291 recombinant hDAO (rhDAO) carry complex-type glycans, whereas Asn-110 carries only mammalian-atypical

292 Herein, a novel glycan with an unusual chemical composition and structur

293 he HA190 mutant bound to a broad spectrum of glycans with alpha2,6/8/9-linked sialic acids.

294 ase Ar) and show that both enable release of glycans with more sugar residues on the proximal GlcNAc

295 Asn-538 and Asn-745 had similar complex-type glycans with some tissue- and cell line-specific variati

296 Moreover, glycans with unique glycosidic linkages, particularly fr

297 nalpha1-2Man on two different termini of the glycan, with the reducing-end mannose residue ligated to

298 reduce their immunogenicity by introducing N-glycans within the V3 region of BG505 SOSIP trimers.

299 The reduced N-glycans, without being permethylated, were also separate

300 c plant-specific sugar residues on protein N-glycans, yielding approximately 1 mg purified moss-deriv