ライフサイエンスコーパス: glycolate

1 h as dihydroxyacetone phosphate, malate, and glycolate.

2 ted against the interleukin-2 receptor, with glycolate.

3 s accompanied with lesser ability to process glycolate.

4 photorespiratory intermediates, glycine and glycolate.

5 way evolved to prevent accumulation of toxic glycolate.

6 Low concentrations of glycolate (12-48 nmol/g) and PG (4-17 nmol/g) were detec

7 mediate stage in method development, 1 pg of glycolate-2,2,-d(2) could be detected by subjecting it t

8 ing the following sequence of steps: (1) add glycolate-2,2-d(2) as an internal standard and exchange

9 The pathway recycles glycolate-2-phosphate, the most abundant byproduct of Ru

10 analytes for oxidative sugar damage to DNA: glycolate, 3-hydroxy-2-butanone, 3-phenylbutyraldehyde,

11 e inhibitor also caused the cells to excrete glycolate, a photorespiratory intermediate, but did not

12 ed an additive growth defect, an increase in glycolate accumulation, and reductions in photosynthetic

13 ochemical outcome of the Lewis acid-mediated glycolate addition of the titanium enolates from protect

14 ct with enolates and enones to afford either glycolate aldol or Michael adducts.

15 A glycolate aldol reaction provided a diene useful for rin

16 ropyran, a dinuclear Zn-catalyzed asymmetric glycolate aldol reaction to prepare the syn 1,2-diol, an

17 include the use of two new asymmetric boron glycolate aldol reactions.

18 low for the preparation of cross-benzoin and glycolate-aldol products in high yield and with exceptio

19 a novel asymmetric phase-transfer-catalyzed glycolate alkylation reaction.

20 c decision to rely heavily on the asymmetric glycolate alkylation to construct both the C1-C14 fragme

21 arpless kinetic resolution and an asymmetric glycolate alkylation to establish the stereogenic center

22 Asymmetric glycolate alkylation using a protected acetophenone surr

23 oxypropionic acid 26 that was made using PTC glycolate alkylation.

24 n hydrogen/deuterium isotope effect and that glycolate alpha-deprotonation itself was only partially

25 We found that exogenous glycolate also rescued photoheterotrophic growth of CT01

26 erevisiae Tdp1, catalyzed similar removal of glycolate, although less efficiently than removal of tyr

27 Glycolate and 2-phosphoglycolate (PG) are 2-carbon monoc

28 Labeling cells with 14C-glycolate and chromatographic analyses indicated that de

29 latter results in the ubiquitous metabolites glycolate and glucosamine 6-phosphate.

30 The mutant accumulates glycolate and glycerate, leading to the hypothesis that

31 is paper reports an additional pathway for P-glycolate and glycolate metabolism in the chloroplasts.

32 ultimately prove to be useful for decreasing glycolate and glyoxylate levels in primary hyperoxaluria

33 Glycolate and P-glycolate were oxidized about equally we

34 s spectrometry (IC-MS) to quantify levels of glycolate and PG in tissue.

35 s of the origin, function, and metabolism of glycolate and PG in tissues.

36 ation is essential for the detoxification of glycolate and recycling of carbon to the Calvin Benson B

37 ylase, increased accumulation of glycine and glycolate and reduced levels of sucrose.

38 D-glycerate, the reduction of glyoxylate to glycolate and the oxidation of D-glycerate to hydroxypyr

39 The plasma concentration of glycolate and the urinary excretion of oxalate, the majo

40 constant for capture of glycolate (k(cat)/K(glycolate)) and the K(d)((app)) for the formation of the

41 ry pathway intermediates 2-phosphoglycolate, glycolate, and glycine, suggest that the deregulation of

42 e unstable 3,3-difluorocyclopropenylcarbinyl glycolates arising from the difluorocyclopropenation.

43 nce in glycolate using natural or deuterated glycolate as a substrate.

44 shed thylakoids from spinach leaves oxidized glycolate at a rate of 22 mol per mg of chlorophyll per

45 is most active on the two-carbon substrate, glycolate, but is also active on 2-hydroxy fatty acids.

46 nnosamine and N-glycolylglucosamine (but not glycolate) can serve as precursors for biosynthesis of e

47 e involvement of only one oxygen atom of the glycolate carboxylate and the product DDP tetrahedral ph

48 The ability of glycolate, CO2, or DMSO to support photoheterotrophic gr

49 concentrations and markedly elevated plasma glycolate concentrations (> or =97.7 mg per deciliter [1

50 l injury during treatment; all 10 had plasma glycolate concentrations at or below 76.8 mg per decilit

51 ALN-GO1 also increased urinary glycolate concentrations in normal nonhuman primates and

52 coding glycolate oxidase and increased serum glycolate concentrations in wild-type mice, rats, and no

53 For example, background contamination by glycolate could increase rather than decrease when the m

54 metabolic pathway, we developed an indirect glycolate cytotoxicity assay in a 1,536-well plate forma

55 ed levels of RNA for genes encoding putative glycolate degradation functions.

56 Particularly, the pivaloyl protected glycolate derivative provides the reluctant anti adducts

57 wide array of beta-stereogenic tertiary aryl glycolate derivatives with high levels of diastereo- and

58 de catalyst is effective for the addition of glycolate-derived silyl ketene acetals to aldehydes.

59 ified." The work extends the sensitivity for glycolate detection by approximately 100-fold and provid

60 SHAM stimulated severalfold glycolate excretion by algal cells, Dunaliella or Chlamy

61 ata indicate that BASS6 and PLGG1 partner in glycolate export from the chloroplast, whereas PLGG1 alo

62 ribution, pharmacokinetics and catabolism of glycolated Fab (glyco-Fab) were evaluated at two differe

63 with various numbers of glycolate molecules [glycolated Fab fragments (glyco-Fabs)] and separated the

64 nvolvement in the photorespiratory export of glycolate from Arabidopsis chloroplasts.

65 n the presence of EDTA, catalyzed removal of glycolate from phosphoglycolate at a single-stranded 3'

66 Tdp1 has also been shown to remove glycolate from single-stranded DNA containing a 3'-phosp

67 ase activities together with accumulation of glycolate further implied that SHR deficiency impacts th

68 expression analyses, we identified Plastidal glycolate glycerate translocator 1 (PLGG1) as a candidat

69 s double knockout line of both BASS6 and the glycolate/glycerate transporter PLGG1 (bass6, plgg1) sho

70 s indicate that PLGG1 is the chloroplastidic glycolate/glycerate transporter, which is required for t

71 e, leading to the hypothesis that PLGG1 is a glycolate/glycerate transporter.

72 The kinetic parameters for the oxidation of glycolate, glyoxylate, and 2-hydroxy octanoate indicate

73 P-glycolate has been considered to be metabolized exclusiv

74 Incubation of these cells with glycolate has revealed that a pathway may function to sy

75 icle antioxidant properties of poly(ethylene glycolated) hydrophilic carbon clusters (PEG-HCCs) by el

76 isotope effects using natural and deuterated glycolate in either natural or deuterated solvent.

77 examines pathways of oxalate synthesis from glycolate in Hep G2 cells, a human hepatoma cell line.

78 uses l-lactate with a similar efficiency to glycolate; in contrast, the photorespiratory isoforms GO

79 d to identify compounds that reduce indirect glycolate-induced cytotoxicity by either enhancing AGT a

80 safety and antiviral effects of polyethylene glycolated interleukin-2 (PEG-IL-2) and thymosin alpha 1

81 which accepts electrons from glycolate or P-glycolate is a quinone after the DCMU site but before th

82 in which X(-) is a sacrificial anion such as glycolate, isethionate, or nitrate.

83 he second-order rate constant for capture of glycolate (k(cat)/K(glycolate)) and the K(d)((app)) for

84 k(cat)/K(m) = 4 x 10(2) M(-1) s(-1)), acetyl glycolate (k(cat)/K(m) = 1.3 x 10(4) M(-1) s(-1)), and u

85 hree polar residues for interaction with the glycolate leaving group.

86 logue of the existing {Pd84 }(Ac) wheel with glycolate ligands, {Pd84 }(Gly) , and the next in a magi

87 m high CO2 to air in light, they accumulated glycolate linearly for 6 h to levels 7-fold higher than

88 leading us to propose that CO2 produced from glycolate metabolism can be used by the Calvin cycle to

89 ts an additional pathway for P-glycolate and glycolate metabolism in the chloroplasts.

90 A detailed examination of glycolate metabolism in these cells should help clarify

91 One 2-phosphoglycolate (P-glycolate) molecule is produced for each O2 molecule fix

92 ti-Tac Fab fragments with various numbers of glycolate molecules [glycolated Fab fragments (glyco-Fab

93 nd PLGG1 therefore balance the export of two glycolate molecules with the import of one glycerate mol

94 e possible site which accepts electrons from glycolate or P-glycolate is a quinone after the DCMU sit

95 Light-dependent glycolate or P-glycolate oxidation by osmotically shocke

96 A replaced the N-terminal 43 amino acids of glycolate oxidase (a peroxisomal protein) was affinity p

97 nctioning independently from NADPH oxidases, glycolate oxidase (GLO) was recently demonstrated as an

98 rotein, and the leaf-type peroxisomal enzyme glycolate oxidase (GLO) were transported into pumpkin (C

99 Glycolate oxidase (GO) and alanine:glyoxylate aminotrans

100 Human glycolate oxidase (GO) catalyzes the FMN-dependent oxida

101 characterized family members, e.g., spinach glycolate oxidase (GOX) and the electron transferases ye

102 miana, and identified the peroxisomal enzyme glycolate oxidase (GOX) as an essential component of non

103 zed members of this protein family including glycolate oxidase (GOX) from spinach.

104 As an exception, glycolate oxidase (GOX) mutants with a photorespiratory

105 Here, we identify the Arabidopsis glycolate oxidase (GOX) paralogs GOX1, GOX2, and GOX3 as

106 -residue segment from its soluble homologue, glycolate oxidase (GOX).

107 Decreased glycolate oxidase and catalase activities together with

108 tructural studies of two homologous enzymes, glycolate oxidase and flavocytochrome b(2), indicated th

109 , and durable silencing of the mRNA encoding glycolate oxidase and increased serum glycolate concentr

110 of the mechanism and possible differences in glycolate oxidase enzyme chemistry from C3 and C4 plant

111 High levels of active glycolate oxidase from spinach (GO) and active catalase

112 ound MDH are replaced by residues 176-195 of glycolate oxidase from spinach.

113 Glycolate oxidase is a flavin-dependent, peroxisomal enz

114 In plants, glycolate oxidase is involved in the photorespiratory cy

115 effect was also investigated for each plant glycolate oxidase protein by measuring the (13)C natural

116 lpha-hydroxy acid oxidases including spinach glycolate oxidase, a loop region, known as loop 4, is co

117 ith significant sequence similarity to plant glycolate oxidase, a prototypical 2-hydroxy acid oxidase

118 in situ production by chloroplast-localized glycolate oxidase, implying that H2O2 can activate CEF e

119 NA interference (RNAi) therapeutic targeting glycolate oxidase, to deplete the substrate for oxalate

120 the oxidation of glycolate to glyoxylate by glycolate oxidase.

121 ic steps of the catalytic mechanism of human glycolate oxidase.

122 A mutant in the maize (Zea mays) Glycolate Oxidase1 (GO1) gene was characterized to inves

123 d a second-site loss-of-function mutation in GLYCOLATE OXIDASE1 (GOX1) that attenuated the photorespi

124 Light-dependent glycolate or P-glycolate oxidation by osmotically shocked chloroplasts

125 This glycolate oxidation is a light-dependent, SHAM-sensitive

126 Glycolate oxidation was assayed with 3-(3,4)-dichlorophe

127 oup acting as a base in the chemical step of glycolate oxidation.

128 ometry of photorespiratory CO2 formation per glycolate oxidized normally increases at higher temperat

129 All three glycolate oxygens coordinate the two Mn(2+) atoms.

130 ng induction of malic enzymes, ppsA, and the glycolate pathway and repression of glycolytic and gluco

131 a pharmacologically optimized, polyethylene-glycolated (PEG)-leptin analog in combination with exend

132 l emphasis is given to sol-gel processes via glycolated precursor molecules as well as the miniemulsi

133 dation is a light-dependent, SHAM-sensitive, glycolate-quinone oxidoreductase system that is associat

134 Moreover, glycolate-removing activity could be immunodepleted from

135 reogenic centers, including a highly unusual glycolate residue.

136 nd releases repaired proteins and lactate or glycolate, respectively.

137 Feeding of butyrate, isobutyrate and glycolate results in the production of 3-hydroxyhexanoat

138 Here, we show that the use of glycolated side chains on a thiophene backbone can resul

139 Pretreating arterioles with polyethylene glycolated--SOD (PEG-SOD) improved vasodilation to ACh a

140 Concurrent administration of polyethylene-glycolated superoxide dismutase (SOD), l-nitroarginine m

141 hway may function to synthesize oxalate from glycolate that does not depend on the oxidation of glyco

142 thylenetriamines with the triflate of benzyl glycolate, thereby minimizing competitive lactamization,

143 GO) catalyzes the FMN-dependent oxidation of glycolate to glyoxylate and glyoxylate to oxalate, a key

144 ate that does not depend on the oxidation of glycolate to glyoxylate by glycolate oxidase.

145 ation biocatalyst used for the conversion of glycolate to glyoxylate in the presence of a reaction co

146 oxy octanoate indicate that the oxidation of glycolate to glyoxylate is the primary reaction catalyze

147 Providing [1-14C]glycolate to leaf tissue of go1 mutants in darkness conf

148 ng for enzymes involved in the conversion of glycolate to ribulose-1,5-bisphosphate.

149 lite analysis and genetic complementation of glycolate transport in yeast showed that BASS6 was capab

150 rt in yeast showed that BASS6 was capable of glycolate transport.

151 by measuring the (13)C natural abundance in glycolate using natural or deuterated glycolate as a sub

152 o fully overcome background contamination by glycolate was unsuccessful.

153 uence applied to readily available propargyl glycolates was developed as a route toward functionalize

154 s little as 10 pg of standard glycolic acid (glycolate) was detected in a method comprising the follo

155 where replacing Bpin with Beg (eg = ethylene glycolate) was predicted to significantly improve ortho

156 Glycolate and P-glycolate were oxidized about equally well to glyoxylate

157 ely established but is suggestive of a bound glycolate, which is isosteric with a glycine (Xaa) produ

158 hich share similar enzymatic properties, use glycolate with much higher efficiencies than l-lactate.

159 thylsilyl ketene acetals derived from methyl glycolates with a large protecting group on the alpha-ox

160 e ketal developed by Ley provided alpha-aryl glycolates with excellent diastereoselectivities (90 to