ライフサイエンスコーパス: glycosylation

1 ly charged residues such as Arg suppressed N-glycosylation.

2 PMM2 encodes a key enzyme in N-glycosylation.

3 is any potential contribution from O-linked glycosylation.

4 hat mask potential phage binding sites using glycosylation.

5 ole of TRAP in human congenital disorders of glycosylation.

6 litate detection of micro-heterogeneity of N-glycosylation.

7 f the C3/C5 diol with boronic acids prior to glycosylation.

8 lycosylation profile at sites with divergent glycosylation.

9 he benzoxazinoid breakdown product MBOA by N-glycosylation.

10 presence of the NHAc functional group during glycosylation.

11 re modified post-translationally by O-linked glycosylation.

12 (SRD5A3-CDG) is a rare disorder of N-linked glycosylation.

13 or a better understanding of OST-catalyzed N-glycosylation.

14 ic acids and proteins, both with and without glycosylation.

15 protein production variables can impact Env glycosylation.

16 ound to be imperative for cdh and pcdh O-Man glycosylation.

17 s assembled into cellulase complexes through glycosylation.

18 its reliance on mechanical force and protein glycosylation.

19 Es in a Diels-Alder reaction and a catalytic glycosylation.

20 o cyclization, and 1,2-trans stereoselective glycosylation.

21 mal cellular trafficking depends on N- and O-glycosylation.

22 ith additional effects on alpha dystroglycan glycosylation.

23 imidates interact with various promoters of glycosylation.

24 residues, forming multiple types of protein glycosylation.

25 to the mechanism by which radiation modifies glycosylation.

26 eveloped as a new platform for active-latent glycosylations.

27 ariables exert the greatest influence on Env glycosylation?

28 plasma membrane localization of FLA4, with N-glycosylation acting at the level of ER-exit and O-glyco

29 was prone to unfolding and exhibited reduced glycosylation activity against a complex array of glycos

30 Here, we investigated whether glycosylation acts as a barrier to infection for other n

31 Here, we tested whether glycosylation also acts as a determinant of permissivity

32 Because glycosylation alters the affinity of antibodies for Fc r

33 ing downregulates Shisa2, which inhibits the glycosylation and cell surface presentation of Frizzled3

34 ization of tetrazolopyrimidines, followed by glycosylation and cross-couplings or nucleophilic substi

35 Comparison of N-linked glycosylation and cysteine mutant replication kinetics i

36 er, little is known about the alterations of glycosylation and glycoproteins in ASD.

37 l bioactivity profile influenced by compound glycosylation and growth phase of cultured cells.

38 these results clarify the role of core gp120 glycosylation and illustrate a general method for design

39 cle tissues, an overall recovery of alpha-DG glycosylation and improved muscle strength, suggesting a

40 of the vector induced a decrease of alphaDG glycosylation and laminin binding, even in WT animals.

41 erations in hexosamine biosynthetic pathway, glycosylation and lipid metabolism as crucial for cancer

42 The identification of O-glycosylation and N-terminal cleavage as novel structura

43 latory connection between nutrient-sensitive glycosylation and NRF2 signaling and provide a blueprint

44 t screening of natural products for specific glycosylation and pathogen interactions.

45 Using in vitro O-glycosylation and proteolytic cleavage assays, a cell li

46 the Asn(297) mutants that result in lack of glycosylation and thus loss of effector function, we dem

47 rolling the location of proteins involved in glycosylation and vesicle formation, our data reveal the

48 Mass spectrometry revealed glycosylations and phosphorylations as expected from fun

49 d extensive posttranslational modifications (glycosylation), and they have been implicated in many bi

50 ycan substrates, 3-4 d to engineer the IgG N-glycosylation, and 2-5 d to synthesize the small-molecul

51 e completely orthogonal to the gold-promoted glycosylation, and an iterative synthesis of oligosaccha

52 eas is not due to reduction in total surface glycosylation, and for tissue paper blotting is likely d

53 ecause of the high sequence diversity, heavy glycosylation, and inherent meta-stability of Env.

54 racer design modifications included chelate, glycosylation, and radiometal.

55 odel confirm that genes involved in N- and O-glycosylation are modulated by radiation, and in silico

56 the molecular mechanisms underlying protein glycosylation are still poorly understood.

57 reactions, and in both cases, the orthogonal glycosylations are carried out utilizing catalytic activ

58 Therefore, here we performed in vitro glycosylation assays and cell culture experiments to com

59 l information for enhancing or suppressing N-glycosylation at a site of interest and valuable data fo

60 in-1 also exclusively displayed high-mannose glycosylation at Asn-137.

61 We report here that glycosylation at Asn-297 is critical for interactions wi

62 ns with Fc receptors and complement and that glycosylation at Asn-563 is essential for controlling mu

63 at the G158N mutation introduced an N-linked glycosylation at positions 158 to 160 of the HA protein

64 This study identifies beta1AR N-terminal O-glycosylation at Ser(37)/Ser(41) as a mechanism that pre

65 ependent Dictyostelium development, but full glycosylation at that position is required for optimal O

66 modulating virus release and virulence, and glycosylation at the catalytic domain affects its thermo

67 th the addition of covalently linked sugars (glycosylation) being one of the most abundant modificati

68 We propose that differences in glycosylation between H3N2 egg-adapted vaccines and circ

69 otein RFT1 is essential for normal protein N-glycosylation, but its precise function is not known.

70 N-glycosylations can regulate the adhesive function of int

71 insect cells, which has enhanced its protein glycosylation capabilities.

72 Glycosylation changes associated with cellular transform

73 Progress in glycosylation chemistry and the development of effective

74 d stereo- or regioselectivity under standard glycosylation conditions.

75 ases (GalNAc-Ts), that initiate mucin-type O-glycosylation, consist of a catalytic and a lectin domai

76 very may reveal potential novel functions as glycosylation could influence their conformation and bio

77 However, a glycosylation defect that results in N-terminal truncati

78 In this study, we report a novel glycosylation-dependent mechanism that protects tumor ce

79 These collective findings point to a novel glycosylation-dependent mechanism that regulates the cel

80 SRD5A3 and other glycosylation disorder genes should be considered as a c

81 Proper gp160 folding in the ER requires core glycosylation, disulfide-bond formation and proline isom

82 ay among FUT8, GnT-IV, and GnT-V in N-linked glycosylation during the assembly of glycoproteins.

83 ate the influence of sequence variation on N-glycosylation efficiency in the context of a five-residu

84 We devised an algorithm for prediction of N-glycosylation efficiency using the SAS software, employi

85 side chains at the i-1 position had higher N-glycosylation efficiency, and Cys, in particular, compen

86 ning residues at the i-2 position improved N-glycosylation efficiency, while positively charged resid

87 160 of the HA protein and that this N-linked glycosylation enhanced viral productivity in infected ma

88 of an open Skp1 conformation can explain how glycosylation enhances interactions with F-box proteins

89 tants of all hitherto characterized extensin glycosylation enzymes; both root hair and glycan phenoty

90 This glycosylation event enhanced viral productivity, exacerb

91 e neighboring group participation during the glycosylation events.

92 asis, resulting in global changes in protein glycosylation, expression and functional effects.

93 side chains represent the two major protein glycosylation forms.

94 unique long-range N- and/or C-terminal prior glycosylation (GalNAc-O-Ser/Thr) preferences modulated b

95 cleavage assays, a cell line deficient in O-glycosylation, GalNAc-T-edited cell line model systems,

96 Abnormal glycosylation has been associated with PKD, and we found

97 lycan knockouts on the CD demonstrate that N-glycosylation has little impact on cellulose conversion

98 e posttranslational modifications, including glycosylation, has not been addressed.

99 s studies on the sequence requirements for N-glycosylation have yielded the Asn-X-Ser/Thr (NXS/T) seq

100 Here, we review the process of antibody glycosylation, how it changes with disease, how it impac

101 Although glycosylation improves solubility and renders the metabo

102 eport illustrates the importance of N-linked glycosylation in modulating the response of cancer cells

103 vide a rationale for the prevalence of pilus glycosylation in nature.

104 ndogenous insect cell gene and alter protein glycosylation in the BICS.

105 indings demonstrate a new role for bisecting glycosylation in the control of Notch transport and demo

106 Glycosylation in the endoplasmic reticulum (ER) is close

107 lays a direct or indirect role in GPI anchor glycosylation in the Golgi apparatus.

108 ing at the S1/S0 site is blocked by O-linked glycosylation in third instar larvae.

109 ylation acting at the level of ER-exit and O-glycosylation influencing post-secretory fate.

110 es is that they do not provide site-specific glycosylation information.

111 l treatments and highlights the potential of glycosylation inhibitors as future combination treatment

112 perone BiP, while only mutants with aberrant glycosylation interact with calreticulin.

113 N-glycosylation involves the attachment of an oligosacchar

114 ies for Fc receptors, evidence suggests that glycosylation is a central mechanism for the immune syst

115 Glycosylation is a fundamental cellular process that, in

116 Reversible glycosylation is also dynamic in biological systems.

117 N-Linked protein glycosylation is an essential and highly conserved post-

118 Since protein glycosylation is an important determinant of cell adhesi

119 sequence can also block phage infection, but glycosylation is considered less likely to create disadv

120 A hallmark of protein N-glycosylation is extensive heterogeneity associated with

121 Protein glycosylation is highly diverse and essential for mammal

122 We found that N-linked glycosylation is important to the regulation of HSV-1-in

123 Mucin-type O-glycosylation is initiated by a large family of UDP-GalN

124 rrant GalNAc-T6 expression and site-specific glycosylation is involved in oncogenic transformation.

125 lling the balance of oxazoline formation and glycosylation is key to achieving acceptable chemical yi

126 Corneal surface glycosylation is modulated by IL-1R and Pseudomonas aeru

127 VR is an N-linked glycosylated protein, this glycosylation is not a strict requirement for AAV2 bindi

128 initiation of cdh/protocadherin (pcdh) O-Man glycosylation is not dependent on the evolutionary conse

129 Our data indicate that O-glycosylation is not necessary for the natural replicati

130 The glycosylation is shown to occur by an effectively concer

131 We show that IgG N-glycosylation loci are strongly enriched for genes expre

132 uggesting the existence of yet another O-Man glycosylation machinery.

133 This report also defines the sites where glycosylation may be impacted when Env trimers are trunc

134 glycosylated insulin analogues, this natural glycosylation may provide important insights into diabet

135 r co-regulatory functions of site-specific O-glycosylation mediated by a unique GalNAc-T isoform.

136 ns in the DAO structure, Asn-538 and Asn-745 glycosylations might be important for efficient DAO dime

137 ich doesn't have electrically non-conductive glycosylation moiety, was immobilized on carbon nanotube

138 in 1 subunit B bound to ten EWs with similar glycosylation more intensely than pigeon EW.

139 Interestingly, our data showed that the gL glycosylation mutant increases the fusion activity of th

140 Our previous study found that the gL glycosylation mutant N69L/S71V had an epithelial cell-sp

141 mined the role of the two conserved N-linked glycosylation (N48 and N58) sites of gK in virus-induced

142 ruit and leaves in particular, demonstrating glycosylation occurred after grapevine treatment; howeve

143 4-(N-ribofuranosyl)-6-aminopyrimidines 4 via glycosylation of 4-aminopyrimidines 2 or 5 is described.

144 hetic approach also allowed a regioselective glycosylation of 6-OH of aspicilin with d-desosamine, a

145 rmined that mOGT is probably involved in the glycosylation of a restricted set of mitochondrial targe

146 Koenigs-Knorr glycosylation of acceptors with more than one free hydro

147 ystroglycanopathies, because deficient O-Man glycosylation of alpha-dystroglycan disrupts laminin int

148 mannosylation pathway involved in functional glycosylation of alpha-dystroglycan gives rise to congen

149 but its function was impacted since abnormal glycosylation of alphaDG was observed.

150 antibody responses that are not affected by glycosylation of antigenic site B of H3N2 HA.

151 Blocking complex glycosylation of Env affected V2 bnAb recognition, as pr

152 ection in SLOs by Delta5G also suggests that glycosylation of Env modulates innate/inflammatory respo

153 These results suggest that the glycosylation of Env modulates the inflammatory/Th1 resp

154 ic reticulum possibly by preventing N-linked glycosylation of Fz8.

155 ivity, as well as variable O-acetylation and glycosylation of GlcNAc contribute to the structural div

156 ioglycoside donors, followed by a late-stage glycosylation of heptagalactan backbone acceptors to int

157 Glycosylation of IgG is known to modulate the effector f

158 ed by LC tandem MS analysis to determine the glycosylation of Influenza hemagglutinin (H1/A/Californi

159 In addition, O-Man glycosylation of IPT/TIG domains of plexins and hepatocy

160 N-glycosylation of Lhs1 is largely Ost3-independent and in

161 domain affects its thermostability; however, glycosylation of M2 had no effect on its function.

162 Selected mutations also lead to partial glycosylation of N582, suggesting that rapid folding of

163 Glycosylation of NA in the stalk region affects its stru

164 hannel to catalyze co-translational N-linked glycosylation of proteins in the endoplasmic reticulum.

165 ress in the ER inhibits the Ost3-dependent N-glycosylation of Sil1, which regulates specific BiP func

166 or glycosylation step with an amine-mediated glycosylation of the boronic ester, a variety of selecti

167 Our studies suggest glycosylation of the E protein is an important factor co

168 nity hFcgammaRs, demonstrating that N-linked glycosylation of the Fc is not a strict requirement for

169 rapid folding of local conformation prevents glycosylation of this site in wild-type FVIII.

170 ansferase has apparently evolved to increase glycosylation of TSRs, potentially to increase the stabi

171 Glycosylation of uterine endometrial cells plays importa

172 Glycosylations of 4,6-tethered glucosazide donors with a

173 atalysts to control site-selectivity for the glycosylations of complex polyols such as 6-deoxyerythro

174 O-Linked glycosylation often involves the covalent attachment of

175 Here, we examine N- and O-glycosylation on a model, multimodular glycoside hydrola

176 rved POMT1/POMT2 enzymes that initiate O-Man glycosylation on alpha-DG.

177 The glycosylation on Env is influenced by a variety of facto

178 a mechanism in which the first step involves glycosylation on glutamate.

179 ontrast, little is known about the effect of glycosylation on IgM binding to the human Fcmu receptor

180 nabling the first large-scale discovery of O-glycosylation on signaling peptides.

181 is study investigated the effect of N-linked glycosylation on the binding of Herceptin to HER2 protei

182 High regioselectivity for the glycosylation on the equatorial hydroxyl group of the ac

183 The effects of glycosylation on the structure and dynamics of NMDARs ar

184 This glycosylation-optimized factor H showed full in vitro co

185 sted that Glt1 belongs to the ancestral Skp1 glycosylation pathway in protists and evolved separately

186 conditions, the prevalence of this extrinsic glycosylation pathway in vivo remains unknown.

187 PUT1), which is the first enzyme in the GIPC glycosylation pathway.

188 early remodeling of glycans in the mammalian glycosylation pathway.

189 nical rules developed for other eukaryotic N-glycosylation pathways, raising questions as to the basi

190 in expressed blue color (pH 7-8), suggesting glycosylation pattern also plays a role.

191 hypothesized that radiation could alter the glycosylation pattern of endothelial cells and thereby i

192 The glycosylation pattern of mAbs is considered to be an imp

193 profile comprised of protein expression and glycosylation pattern was achieved in simple protocol wi

194 ometry (MS) for the qualitative profiling of glycosylation patterns at the protein level, by comparin

195 s on Notch1 result from generation of unique glycosylation patterns on Notch1.

196 authors conduct a multivariate GWAS on IgG N-glycosylation phenotypes and identify 5 novel loci enric

197 ation studies of 23 immunoglobulin G (IgG) N-glycosylation phenotypes.

198 re, mutagenesis studies demonstrate that the glycosylation plays an essential role in the flagellar a

199 We found that, while glycosylation plays an important role in these orthologs

200 Protein N-glycosylation (PNG) is crucial for protein folding and e

201 Heterogeneity of protein glycosylation poses great challenges for analysis that i

202 uctural basis for the different remote prior glycosylation preferences of the GalNAc-Ts.

203 linker constructs show altered remote prior glycosylation preferences, confirming that the flexible

204 ogical analysis of full-length NMDARs with a glycosylation-preventing GluN1-N440Q mutation, and demon

205 Among other genes involved in the alpha-DG glycosylation process, fukutin related protein (FKRP) ge

206 r results implicate RFT1 in a wider range of glycosylation processes than previously appreciated.

207 etry (MS)-based approach to map the complete glycosylation profile at every site in multiple HIV-1 En

208 e identified the variables that impact Env's glycosylation profile at sites with divergent glycosylat

209 e identified numerous sites on Env where the glycosylation profile does not deviate in 11 different E

210 This report provides the first consensus glycosylation profile of Env trimers, which should serve

211 t facilitate characterizing and monitoring a glycosylation profile, albeit mainly at a glycan and gly

212 we investigated the impact on the divergent glycosylation profiles of changing the genotype of the E

213 etin alpha products, reputed to have similar glycosylation profiles.

214 The glycosylation reaction was promoted by the inexpensive C

215 In particular, stereochemical outcomes in glycosylation reactions are highly dependent on the ster

216 In glycosylation reactions using superarmed donors, both se

217 hree- and four-component, one-pot sequential glycosylation reactions, and in both cases, the orthogon

218 ong the donors were evaluated in competitive glycosylation reactions, and their relative reactivities

219 cosyltransferases, the enzymes mediating the glycosylation reactions, are located within the Golgi lu

220 ation of transient orthoesters formed during glycosylation reactions, depending on the nature of the

221 ser to the assumed transition state in these glycosylation reactions.

222 sphate, an obligate lipid carrier in protein glycosylation reactions.

223 s), such as phosphorylation, acetylation and glycosylation, regulate numerous cellular processes, pla

224 ions, specifically the degree and pattern of glycosylation, regulate the oligomeric state of the prot

225 h Sil1 and Lhs1 are glycoproteins, but how N-glycosylation regulates their function is not known.

226 lly utilized bacterial expression, including glycosylation required for antibody functionality and ma

227 show that the normal distribution of surface glycosylation requires IL-1R, but not MyD88, and is not

228 embryo and protects against infection, with glycosylation responsible for binding certain pathogens.

229 lls in tunicamycin (an inhibitor of N-linked glycosylation) resulted in an increase in sensitivity to

230 he reaction mechanism for the CPA-controlled glycosylations revealed the involvement of covalently li

231 ointestinal tract, kidney, and placenta, and glycosylation seems essential for reaching high enzyme e

232 macrolactonization, a combination of in vivo glycosylation, selective in vitro cytochrome P450-mediat

233 urprisingly does not possess the canonical N-glycosylation sequence N-X-S/T.

234 X-S/T (X not equal P), a motif known as an N-glycosylation'sequon'.

235 r dystrophy with a reduction in dystroglycan glycosylation, short stature, intellectual disability, a

236 EW glycosylation similarity among the different orders of b

237 ed for extracting DIA data is common to that glycosylation site and not dictated by a specific MS1 va

238 nsitive to the presence of a single N-linked glycosylation site at asparagine 297 of the Fc, with deg

239 ion of an additional fully occupied N-linked glycosylation site at the N terminus at position 1 (equi

240 in PNS myelin and mutations in which at the glycosylation site cause Charcot-Marie-Tooth neuropathy,

241 C.2a H3N2 viruses possessing a new predicted glycosylation site in antigenic site B of HA emerged, an

242 sequence motif, VNDT, containing an N-linked glycosylation site in the envelope (E) protein, is polym

243 DT motif is deleted or in which the N-linked glycosylation site is mutated by single-amino-acid subst

244 irus-expressed H3 antigens (that possess the glycosylation site motif) were able to efficiently recog

245 e how hemophilia mutations near the unused N-glycosylation site of the A2 domain (N582) of FVIII affe

246 sites, a third novel, albeit low abundant, N-glycosylation site on C9 is identified, which surprising

247 Specifically, mDPP4 has a nonconserved glycosylation site that acts as a barrier to MERS-CoV in

248 rom the recent outbreaks contain an N-linked glycosylation site within the viral envelope (E) protein

249 lating H3N2 viral strain (that possesses the glycosylation site) and humans vaccinated with baculovir

250 Remarkably, the glycan patterns, glycosylation site, and their occupancy by N-glycans are

251 extensive heterogeneity associated with each glycosylation site.

252 of this viral strain lacks the new putative glycosylation site.

253 in the stalk region abolishing the N-linked glycosylation site; and 2 variants represented the head

254 esults demonstrate a critical role for the N glycosylation sites and cysteines for the structure and

255 -III, di-sialylated glycans at multiple A1AT glycosylation sites and desialylated A2HSG, and depleted

256 Promyeloperoxidase hosts five occupied glycosylation sites and six intrachain cystine bridges w

257 We recently reported that the N-glycosylation sites Asn-168, Asn-538, and Asn-745 in rec

258 (i) We determined which glycosylation sites contain conserved glycan profiles ac

259 Six glycosylation sites fitting the sequon N-X-S/T were succ

260 Previous attempts to alter glycosylation sites in Env typically involved mutating t

261 likely to predominate at all the putative N-glycosylation sites in the parasite proteome.

262 ated from those species present at the other glycosylation sites on the molecule.

263 riable regions (V1-V5), and also at N-linked glycosylation sites that contribute roughly half the mas

264 Additionally, next to the two known glycosylation sites, a third novel, albeit low abundant,

265 red for histamine catabolism, has multiple N-glycosylation sites, but their roles, for example in DAO

266 re were no differences in protease cleavage, glycosylation sites, signal peptides or trans-membrane d

267 Therefore, for proteins containing multiple glycosylation sites, the individual glycan species prese

268 rved N-glycan or the deletion of predicted O-glycosylation sites.

269 acid substitutions and continual addition of glycosylation sites.

270 Ab molecule containing two distinct N-linked glycosylation sites: one present on the heavy chain (HC)

271 lpha-reductase type 3 congenital disorder of glycosylation (SRD5A3-CDG) is a rare disorder of N-linke

272 e-center-cleaved C1INH, but dependent on its glycosylation status.

273 mbining an initial acylation, alkylation, or glycosylation step with an amine-mediated glycosylation

274 pidome; however, our results reveal that the glycosylation steps of GIPC biosynthesis are important r

275 igh yielding, and the stereo outcomes of the glycosylation steps were excellent.

276 sylation activity against a complex array of glycosylation substrates and proteolytic processing of t

277 uires enormous quantities of amino acids and glycosylation sugars to properly build and fold antibodi

278 ta presented here give vaccine developers a "glycosylation target" for their immunogens, and they sho

279 CetuGEX, an antibody with optimized Fc glycosylation targeting the same epitope as cetuximab, r

280 ave been achieved using a one-pot sequential glycosylation technique.

281 We report consensus maps for Cel7A glycosylation that include glycan sites and motifs.

282 rine (the other amino acid that can accept O-glycosylation), the resulting virus was noninfectious.

283 se enzymes are often decorated with N- and O-glycosylation, the roles of which have been broadly attr

284 s a rate-limiting enzyme catalyzing ceramide glycosylation, thereby regulating cellular ceramide leve

285 y divergent eukaryote has re-wired protein N-glycosylation to provide protein sequence-specific N-gly

286 Here, we show that during evolution N-glycosylation triggered a dual selection pressure on sec

287 es through addition/deletion/modification of glycosylation types, sugar types, chemical modifications

288 f post-translational disulfide formation and glycosylation) typically require either expression in ma

289 we explored their effect on corneal surface glycosylation using a metabolic label, tetra-acetylated

290 The copper-promoted glycosylation was also proven to be completely orthogona

291 immuno-PET tracer modifications considered, glycosylation was the most prominent design factor affec

292 Protein glycosylation was variably affected.

293 me the challenges in the analysis of protein glycosylation, we have developed a comprehensive and uni

294 38 influences holoHC by altering haptocorrin glycosylation, whereas B12 bound to non-glycosylated tra

295 ulted in specific loss of cdh and pcdh O-Man glycosylation, whereas combined KO of TMTC1 and TMTC3 re

296 nicated 3,3-difluoroxindole (HOFox)-mediated glycosylations wherein 3,3-difluoro-3H-indol-2-yl (OFox)

297 s a noninvasive tool for investigating brain glycosylation, which could be developed in to brain mapp

298 esolution of the aglycone via stereospecific glycosylation with an enantiopure glycosyl donor followe

299 prepared from monosaccharide stannanes via O-glycosylation with Schmidt-type donors, glycal epoxides,

300 to be reflected in the stereoselectivity in glycosylations with a set of fluorinated alcohols as wel