ライフサイエンスコーパス: glycyl

1 Vmax for apical uptake of [14C]glycyl-[14C]sarcosine was increased 1.64 (+/- 0.34)-fold

2 N-Substituted-glycyl-2-cyanopyrrolidide compounds, typified by NVP-DPP

3 a new DPP-IV inhibitor class, N-substituted-glycyl-2-cyanopyrrolidines, are described as well as the

4 pha-subunit in the complex with an analog of glycyl adenylate at 2.8 A resolution presents a conforma

5 A glycyl-adenylate analogue, glycine sulfamoyl adenosine (

6 The mutation is at the site for synthesis of glycyl-adenylate, but the rest of the two structures are

7 alyzes the stereospecific hydroxylation of a glycyl alpha-carbon in a reaction that requires O(2) and

8 aminolyzes acyclic D-alanyl substrates than glycyl analogues, in contrast to the wild-type beta-lact

9 In addition, both the glycyl and alanyl residues of the GXXXG or GXXXA motifs

10 ntained a constant acyl group, (phenylacetyl)glycyl, and chemically different leaving groups, m-carbo

11 Active-site labeling with dansyl-glutamyl-glycyl-arginyl chloromethyl ketone or immunoblot analysi

12 he adhesion of H10 cells to vitronectin and (glycyl-arginyl-glycyl-aspartyl-serine)4 and significant

13 y higher rates to the polymeric RGD peptide (glycyl-arginyl-glycyl-aspartyl-serine)4 than to monomeri

14 s significantly increases their RGD (arginyl-glycyl-aspartate)-dependent interaction with endothelial

15 o 142 of the pre-pro-protein form an arginyl-glycyl-aspartic (RGD) sequence, a motif involved in the

16 H10 cells to vitronectin and (glycyl-arginyl-glycyl-aspartyl-serine)4 and significant inhibition of t

17 to the polymeric RGD peptide (glycyl-arginyl-glycyl-aspartyl-serine)4 than to monomeric RGD peptides.

18 measurements indicate that the peptidoglycan glycyl bridges are complete (five units long) and form c

19 glyoxylate and the oxidative cleavage of the glycyl Calpha-N bond in N-benzoylglycine (hippurate) by

20 singly, the yields are lower (< 50%) for non-glycyl couplings due to an aza-Wittig reaction that dive

21 the variant with a C-terminal glycyl-glycyl-glycyl-cysteine peptide-based chelator (designated ZHER2

22 tate by the aminolysis product (phenylacetyl)glycyl-D-phenylalanine indicated that the second binding

23 D-alanyl-D-phenylalanine and N-(phenylacetyl)glycyl-D-phenylalanine were used as a test pair against

24 nhibiton of the hydrolysis of (phenylacetyl)-glycyl-D-thiolactate by the aminolysis product (phenylac

25 , RGD-hirudin, and Nalpha-2-naphthylsulfonyl-glycyl-DL-4-amidinophenylalanyl-piperidide acetate salt

26 dyl (Jo-1), threonyl (PL-7), alanyl (PL-12), glycyl (EJ), and isoleucyl (OJ), are closely associated

27 yanate, were removed from partially purified glycyl endopeptidase (PPGE).

28 11(Delta181-381), revealed that the D-alanyl-glycyl endopeptidase activity is contained within the N-

29 ults show that the phi11 enzyme has D-alanyl-glycyl endopeptidase as well as N-acetylmuramyl-L-alanyl

30 O4) precipitation was applied to fractionate glycyl endopeptidase from the papaya latex of Red Lady a

31 Glycyl endopeptidase with high purification fold (PF) an

32 us cysteine proteases papain, actinidin, and glycyl endopeptidase, folding into two distinct domains

33 rolytic activity toward valacyclovir, the 5'-glycyl ester of acyclovir, and the 5'-valyl ester of zid

34 ered proteins (IDPs) containing phenylalanyl-glycyl (FG)-rich repeats line the pore and interact with

35 ged to arginyl-alanyl-aspartic or to arginyl-glycyl-glutamic, were expressed in Escherichia coli.

36 Glycyl-glutamine (3 nmol) also prevented the rise in bod

37 Glycyl-glutamine (3 nmol) co-injection into the mPOA inh

38 In this study, we investigated whether glycyl-glutamine (beta-endorphin(30-31)), an inhibitory

39 Opioid peptides and glycyl-glutamine (Gly-Gln) have been implicated in the c

40 Glycyl-glutamine had no effect on body temperature when

41 stitution of C73 brings about mischarging by glycyl-, glutaminyl-, and leucyl-tRNA synthetases.

42 mor activities of these compounds and their [glycyl,glutamyl] diethyl ester prodrug forms (1(Et)2-3(E

43 ndirectly delivered into tumor cells as the [glycyl,glutamyl] diethyl or dicyclopentyl esters.

44 the fungus, but the control peptide arginyl-glycyl-glutamyl-serine provided no protection.

45 , N(alpha)-acetyl lysine amide, and N(alpha)-glycyl glycine amide are investigated by combined densit

46 ntified based on the inability to use prolyl-glycyl-glycine as a proline source and were defective in

47 , which also serves as the substrate for its glycyl-glycine endopeptidase domain.

48 Using sequential digestion with the glycyl-glycine endopeptidase lysostaphin followed by the

49 is removed by treatment with lysostaphin, a glycyl-glycine endopeptidase that separates the cell wal

50 ine, L-alanyl-glycine, glycyl-L-alanine, and glycyl-glycine, in which we attempt to isolate structura

51 shown to catalyze the prebiotic synthesis of glycyl-glycine, we expect this work will stimulate furth

52 t is a competitive inhibitor of the acceptor glycyl-glycine, which indicates that OU749 occupies the

53 The phenylalanyl-glycyl-glycyl-alanyl-prolyl (FG-GAP) domain plays an imp

54 onstrated that the variant with a C-terminal glycyl-glycyl-glycyl-cysteine peptide-based chelator (de

55 ns containing the photo-tag: 4-benzoylbenzyl-glycyl-hexahistidine.

56 reveals two architectural elements unique to glycyl initiases and critical for their activity.

57 thetase (HisRS) or to alanyl-, asparaginyl-, glycyl-, isoleucyl-, or threonyl-tRNA synthetase occur i

58 Certain glycyl-l(but not d)-amino acids, such as glycyl-l-alanin

59 ain glycyl-l(but not d)-amino acids, such as glycyl-l-alanine and glycyl-l-phenylalanine, are also go

60 tides, L-alanyl-L-alanine, L-alanyl-glycine, glycyl-L-alanine, and glycyl-glycine, in which we attemp

61 n-pimelyl-D-alanyl-D-alanine, 1, contain the glycyl-L-alpha-amino-epsilon-pimelyl side chain.

62 The first is a penicillin having the glycyl-L-alpha-amino-epsilon-pimelyl side-chain of Strep

63 y complexed with a highly specific fragment (glycyl-L-alpha-amino-epsilon-pimelyl-D-Ala-D-Ala) of the

64 , where the enzyme catalyzes transfer of the glycyl-l-alpha-amino-epsilon-pimelyl-d-alanyl moiety to

65 , a peptidoglycan-mimetic peptide substrate, glycyl-l-alpha-amino-epsilon-pimelyl-d-alanyl-d-alanine,

66 hown that the peptidoglycan-mimetic peptide, glycyl-l-alpha-amino-epsilon-pimelyl-d-alanyl-d-alanine,

67 f the Streptomyces R61 dd-peptidase, such as glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-D-alanine,

68 peptidase substrate yielded a new substrate, glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-D-alanine.

69 t overlap with the peptide carbonyl group of glycyl-l-amino acids.

70 es of protonated and alkali metal-cationized glycyl-L-arginine (GlyArg) and L-arginylglycine (ArgGly)

71 c cerebral arteries via binding to L-arginyl-glycyl-L-aspartate-dependent integrin receptors and prev

72 n of an analogous thiolester substrate, 3-(N-glycyl-l-cysteinyl)-propanoyl-d-alanyl-d-thiolactate, th

73 ed by studying the response to the substrate glycyl-L-glutamine (Gly-Gln) of the proton-coupled pepti

74 Glycyl-L-glutamine (Gly-Gln; beta-endorphin 30-31) is an

75 moiety was investigated employing N-benzoyl-glycyl-L-lysine (Bz-Gly-Lys) as a model compound of Lys

76 carboxyethyl) -alpha-(S)-(4-phenyl-3-butynyl)glycyl-L-O-methyltyrosine, N-methylamide), a potent inhi

77 d)-amino acids, such as glycyl-l-alanine and glycyl-l-phenylalanine, are also good acceptors.

78 Glycyl-L-phenylalanine-beta-naphthylamide (GPN) was used

79 e by AsPc-1 and Capan-2 cells was similar to glycyl-L-sarcosine absorption by Caco-2 cells and a Chin

80 Absorption of the model dipeptide glycyl-L-sarcosine by AsPc-1 and Capan-2 cells was simil

81 -3-((13-methyltetradecanoyl)oxy)hexadecanoyl)glycyl-l-serine, abbreviated as l-serine-(R+S)-Lipid 654

82 ential UV-Vis spectroscopy, and bioassays, L-glycyl-L-tryptophan (Gly-Trp) was found to be the only d

83 y titrating a mixture of model fluorophores, glycyl-L-tryptophan and L-tryptophan with Cu2+ at pH 6.

84 I molecules, and we find that the dipeptide glycyl-leucine efficiently supports the folding of HLA-A

85 Treatment of cells with glycyl-leucine induces accumulation of peptide-receptive

86 joined at the normal N and C termini by a di-glycyl linker.

87 ent of (1)H/(2)H exchange into methionine, N-glycyl-methionine, and methionine sulfoxide suggests tha

88 of methionine sulfoxide that is absent for N-glycyl-methionine.

89 AIPPMER), 18-O-[N-(3'-iodo-4'-azidophenethyl)glycyl]methyl reserpate (IAPEGlyMER), and 2-N-[(3'-iodo-

90 tituent that better stabilizes the analogous glycyl moiety.

91 Gly-Lys and was then identified as N-benzoyl-glycyl-Nepsilon-(hexanonyl)lysine.

92 s between hypoxanthine and all standard, non-glycyl/non-prolyl amino acid side chain analogs as deriv

93 de investigated has either a central pyridyl glycyl or a pyridyl alanyl residue between two terminall

94 th one substrate, however, m-[[(phenylacetyl)glycyl]oxy]benzoic acid, isotope effects on V/K of very

95 a depsipeptide substrate, m-[[(phenylacetyl)glycyl]-oxy]benzoic acid, and of a beta-lactam substrate

96 ated with a mixture containing the dipeptide glycyl-proline in Tris-HCl supplemented with manganese a

97 ermined by the complete disappearance of the glycyl-proline peak with the concomitant appearance of t

98 beneath the proline to the area beneath the glycyl-proline peak.

99 activity of the chromogenic substrate tosyl-glycyl-prolyl-argly-4-nitroanilide acetate was present a

100 ass III ribonucleotide reductases (RNRs) are glycyl radical (G*) enzymes that provide the balanced po

101 of an intermediate, tentatively ascribed to glycyl radical and acrylate.

102 e PFL contains a novel alpha-carbon centered glycyl radical at G734 that is required for its catalyti

103 tranded beta/alpha-barrel that surrounds the glycyl radical cofactor and active site.

104 benzylsuccinate synthase complex lacking the glycyl radical could be purified as an alpha(2)beta(2)ga

105 hat the toluene synthase reaction involves a glycyl radical decarboxylase.

106 alphagamma complex center around the crucial glycyl radical domain, thus suggesting a role for BSSbet

107 imethylamine-lyase is an anaerobic bacterial glycyl radical enzyme (GRE) that cleaves choline to prod

108 s, we investigate the catalytic cycle of the glycyl radical enzyme 4-hydroxyphenylacetate decarboxyla

109 mental pollutant toluene is initiated by the glycyl radical enzyme benzylsuccinate synthase (BSS), wh

110 BSS belongs to the glycyl radical enzyme family and catalyzes the fumarate

111 We find that BSSalpha has the common glycyl radical enzyme fold, a 10-stranded beta/alpha-bar

112 ealed the involvement of a C-N bond cleaving glycyl radical enzyme in TMA production, which is unprec

113 nd distribution of individual members of the glycyl radical enzyme superfamily among the microbiomes

114 e determined x-ray crystal structures of the glycyl radical enzyme that catalyzes the addition of tol

115 Benzylsuccinate synthase is a glycyl radical enzyme that initiates anaerobic toluene m

116 zylsuccinate synthase was speculated to be a glycyl radical enzyme.

117 Activation of glycyl radical enzymes (GREs) by S-adenosylmethonine (Ad

118 Glycyl radical enzymes (GREs) represent a diverse superf

119 Recently, BMCs associated with glycyl radical enzymes (GREs) were discovered; these are

120 It is becoming increasingly apparent that glycyl radical enzymes are more prevalent than previousl

121 The glycyl radical enzymes include pyruvate formate-lyase, a

122 primarily on its sequence similarity to the glycyl radical enzymes, pyruvate formate-lyase and anaer

123 cysteine residues that are conserved in all glycyl radical enzymes.

124 Benzylsuccinate synthase is a member of the glycyl radical family of enzymes.

125 sed mechanism in which dioxygen quenches the glycyl radical in the active enzyme and the resulting pe

126 ytic cleavage, resulting in the formation of glycyl radical ions and loss of CH2SO.

127 uced capability to stabilize the thus-formed glycyl radical ions via the captodative effect.

128 cal ions, the stability of the product ions (glycyl radical ions) and the location of the charge (pro

129 e open conformation of PFL in which: (i) the glycyl radical is significantly less stable; (ii) the ac

130 for radical intermediates generated from the glycyl radical of activated pyruvate formate-lyase (PFL)

131 nerates a stable and catalytically essential glycyl radical on G(734) of pyruvate formate-lyase via t

132 me (PFL-AE) involves formation of a specific glycyl radical on PFL by the PFL-AE in a reaction requir

133 onversion of the [4Fe-4S](1+) cluster to the glycyl radical on PFL; however, 3.7-fold less activation

134 the generation of a catalytically essential glycyl radical on pyruvate formate-lyase (PFL).

135 zyme that installs a catalytically essential glycyl radical on pyruvate formate-lyase.

136 aradigm, we demonstrate that generation of a glycyl radical on the B(12)-independent glycerol dehydra

137 edominantly affected by the stability of the glycyl radical product through the captodative effect, r

138 nfortunately, the extreme sensitivity of the glycyl radical to oxygen has hampered the structural and

139 -terminal region of BSSalpha that houses the glycyl radical to shift within the barrel toward an exit

140 exhibits lower catalytic activity; (iii) the glycyl radical undergoes less H/D exchange with solvent;

141 he active site of PFL and harboring a stable glycyl radical, or an open conformation, with Gly-734 mo

142 rements for growth in reactions activated by glycyl radical-containing enzymes.

143 Benzylsuccinate synthase is a member of the glycyl radical-containing family of enzymes, and the rea

144 from the ethanolamine utilization (Eut) and glycyl radical-generating protein MCPs are able to targe

145 m from an enzymatic glycyl residue to form a glycyl radical.

146 ts activity with the concomitant loss of the glycyl radical.

147 of MCPs have been identified, including the glycyl-radical propanediol (Grp) MCP.

148 en tolerance by jettisoning enzymes that use glycyl radicals and exposed low-potential iron-sulfur cl

149 Glycyl radicals are important bioorganic radical species

150 ylsuccinate synthase radical and that of the glycyl radicals of pyruvate formate-lyase and anaerobic

151 the bridging segment was reduced to a single glycyl residue 1.0 +/- 0.1 (FemA), the level of cross-li

152 model dipeptides, each of which contained a glycyl residue and a variable residue.

153 ngly, position 14 of h-alpha-CGRP contains a glycyl residue and is part of an alpha-helix spanning re

154 ontains an organic free radical located on a glycyl residue in the C-terminal portion of the polypept

155 the estimated pKa (approximately 8.0) of the glycyl residue in the high-affinity substrate 1a.

156 lattice, the carbonyl oxygen of the central glycyl residue in two gly-gly-gly polymorphs form interm

157 physiological conditions principally when a glycyl residue is at the carboxyl side of Asn and leads

158 ar to stabilize the Rossmann fold: the first glycyl residue of either the GXXXG or GXXXA motif contac

159 incorporation of this isotope label into the glycyl residue of glutathione at 44.2 ppm can be detecte

160 glycyl residue of these motifs and the third glycyl residue of the GX(1-2)GXXG motif are the same res

161 acts the carbonyl oxygen atom from the first glycyl residue of the GX(1-2)GXXG motif consistent with

162 ither GXXXG or GXXXA motifs, where the first glycyl residue of these motifs and the third glycyl resi

163 bstracting a hydrogen atom from an enzymatic glycyl residue to form a glycyl radical.

164 ent hydroxylation of the alpha-carbon of the glycyl residue, producing an alpha-hydroxyglycine-extend

165 The bridging segment length changed from 5.0 glycyl residues (wild-type strain) to 2.5 +/- 0.1 (FemB)

166 hielding parameters are reported for central glycyl residues in crystallographically characterized tr

167 ns), the excess glycine is used primarily as glycyl residues in protein.

168 iction scheme for the 2 degrees structure of glycyl residues in proteins based on shielding parameter

169 decarboxylated or inserted as (13)C-labeled glycyl residues in proteins.

170 ysine (for a direct measure of the number of glycyl residues in the bridging segment), [1-(13)C]glyci

171 termining step in the Staudinger ligation of glycyl residues mediated by (diphenylphosphino)methaneth

172 cross-bridges containing one, three, or five glycyl residues, but not to the epsilon-amino of lysyl i

173 isolated yields for the ligation of two non-glycyl residues.

174 he order: ryanodol > ryanodine > C(10)-O(eq)-glycyl-ryanodine > C(10)-O(eq)-beta-alanyl-ryanodol.

175 he order: ryanodol < ryanodine < C(10)-O(eq)-glycyl-ryanodine < C(10)-O(eq)-beta-alanyl-ryanodol, imp

176 The currents evoked by 20 mM glycyl-sarcosine (Gly-Sar) at pH 5.0 were dependent upon

177 L296C-, and F297C-hPepT1) showed negligible glycyl-sarcosine (gly-sar) uptake activity and may play

178 g in a high concentration of open N-terminal glycyl segments.

179 Here we report a MOF that consists of glycyl-serine dipeptides coordinated to metal centres, a

180 lpha-hydroxy acids act as acyl acceptors for glycyl substrates but not for more specific d-alanyl sub

181 sidue of desmethylsalinamide E with acylated glycyl thioesters yields desmethylsalinamide C.

182 he activated enzyme and may participate in a glycyl/thiyl radical equilibrium.

183 Evidence for AlmG glycyl to lipid substrate transferase activity is demons

184 , encoding the ubiquitously expressed enzyme glycyl-transfer RNA (tRNA) synthetase (GlyRS).

185 Glycyl tRNA synthetase (GlyRS) provides a unique case am

186 t point mutations in the gene GARS, encoding glycyl tRNA synthetase (GlyRS).

187 disease-associated missense mutations in the glycyl tRNA synthetase gene in families with CMT2D and d

188 itochondrial forms of Caenorhabditis elegans glycyl-tRNA synthetase (CeGlyRS) are encoded by the same

189 ly implicated mutations in the gene encoding glycyl-tRNA synthetase (GARS) as the cause of CMT2D and

190 a unique disease caused by mutations in the glycyl-tRNA synthetase (GARS) gene.

191 throughout the primary sequence of the human glycyl-tRNA synthetase (GARS).

192 a Drosophila model for CMT with mutations in glycyl-tRNA synthetase (GARS).

193 axonal neuropathy, is caused by mutations in glycyl-tRNA synthetase (GARS).

194 ctions may explain why dominant mutations in glycyl-tRNA synthetase (GlyRS) and tyrosyl-tRNA syntheta

195 res and biochemical analyses show that human glycyl-tRNA synthetase (GlyRS) produces Ap4A by direct c

196 Here we focus on mutations in glycyl-tRNA synthetase (GlyRS) that cause an axonal form

197 e gene GARS, encoding the ubiquitous enzyme, glycyl-tRNA synthetase (GlyRS), cause peripheral nerve d

198 in the housekeeping gene GARS, which encodes glycyl-tRNA synthetase (GlyRS), mediate selective periph

199 cerevisiae, two genes (GRS1 and GRS2) encode glycyl-tRNA synthetase (GlyRS1 and GlyRS2, respectively)

200 SR1 glycyl-tRNA synthetase acylates tRNA(Gly)UCA with glycin

201 Pol gamma B shows high similarity to glycyl-tRNA synthetase and dimerizes through an unusual

202 Coexpression of SR1 glycyl-tRNA synthetase and tRNA(Gly)UCA in Escherichia c

203 h a significant conformational transition in glycyl-tRNA synthetase coupled to the binding of GSAd.

204 of horizontal transfer of the gene encoding glycyl-tRNA synthetase from a bacterium of the Thermus-D

205 by the expression of the only known gene for glycyl-tRNA synthetase in Schizosaccharomyces pombe.

206 Unfolding of glycyl-tRNA synthetase is complex with a native state, a

207 erential scanning calorimetry indicated that glycyl-tRNA synthetase is stabilized by nucleotides.

208 Thus, a single gene for glycyl-tRNA synthetase likely encodes both cytoplasmic a

209 ATP and nonsubstrate ATP analogues render glycyl-tRNA synthetase more resistant to digestion by a

210 Unfolding of Bombyx mori glycyl-tRNA synthetase was examined by multiple spectros

211 The interaction of adenine nucleotides with glycyl-tRNA synthetase was examined by several experimen

212 f the Bacillus subtilis glyQS gene (encoding glycyl-tRNA synthetase) can be reproduced in a purified

213 fferent mutant alleles of GARS (the gene for glycyl-tRNA synthetase) have been reported to cause a do

214 mutation in Aats-gly (also known as gars or glycyl-tRNA synthetase), the Drosophila melanogaster ort

215 f the Bacillus subtilis glyQS gene, encoding glycyl-tRNA synthetase, depends on stabilization of an a

216 GARS encodes glycyl-tRNA synthetase, the enzyme that couples glycine

217 AMPPNP and AMPPCP were not substrates for glycyl-tRNA synthetase.

218 ied as GRS1, which encodes the S. cerevisiae glycyl-tRNA synthetase.

219 Interestingly, significant homology to glycyl-tRNA synthetases from prokaryotic organisms revea

220 This fragmentation produces a glycyl-type radical upon losing CH2 SO, and the degree o

221 Herein, we demonstrate that the stability of glycyl-type radicals (X-(.) CH-Y) can be tuned on a mole

222 9), sumatriptan, serotonin-5-O-carboxymethyl-glycyl -tyrosinamide (GTI), 5-methylaminosulfonylmethyl-

223 In the case of glycyl-tyrosine, a stable 3-(1-hydroperoxy-4-oxocyclohex

224 against D-alanyl substrates with respect to glycyl was observed.