ライフサイエンスコーパス: gnotobiotic

1 ora in immunocompetent animals, we generated gnotobiotic and conventionally reared Ig allotype chimer

2 Applying this pipeline to gnotobiotic and human microbiota-colonized mice, we demo

3 iew summarizes the results of developmental, gnotobiotic, and in vitro studies that showed alteration

4 Advances in immunology, gnotobiotics, and culture-independent molecular techniqu

5 ficantly less cariogenic than JH1140 in both gnotobiotic- and conventional-rodent models.

6 roughput anaerobic culturing techniques with gnotobiotic animal husbandry and metagenomics to show th

7 alpha/beta and gamma interferon responses in gnotobiotic animals.

8 and survival of congenitally immunodeficient gnotobiotic beige-athymic (bg/bg-nu/nu) and beige-euthym

9 ibody- and cell-mediated immune responses in gnotobiotic C.B-17 and BALB/c mice.

10 postinfection, all H. saguini-monoassociated gnotobiotic C57BL/129 IL-10(-/-) mice were colonized and

11 n orally inoculated into a BCoV-seronegative gnotobiotic calf, GiCoV-OH3 caused severe diarrhea and v

12 nomes from both the cell culture-adapted and gnotobiotic-calf-passaged strains were also sequenced an

13 gainst the virulent enteric BCoV DB2 strain, gnotobiotic calves (n = 4) were orally inoculated with H

14 e some differences of degree, all inoculated gnotobiotic calves (n = 6) showed abnormal feces between

15 To determine whether HECV-4408 infects gnotobiotic calves and induces cross-protective immunity

16 ervations described previously in studies of gnotobiotic calves and pigs experimentally infected with

17 syncytial virus (RSV) protected the lungs of gnotobiotic calves from RSV infection.

18 Gnotobiotic calves inoculated with fecal filtrates of ea

19 An additional two gnotobiotic calves were inoculated with HECV-4408 and eu

20 In gnotobiotic calves, however, NB virus elicited only diar

21 uated (NCDV/A) bovine group A rotaviruses in gnotobiotic calves.

22 en fixed by BNF to maize and wheat under non-gnotobiotic conditions.

23 t actively suppress Pseudomonas growth under gnotobiotic conditions.

24 mice housed in conventional, germ-free, and gnotobiotic environments.

25 Pigs were maintained in gnotobiotic facility and evaluated for human norovirus (

26 these interactions involved colonization of gnotobiotic Fut2(+) and Fut2(-) mice with Bacteroides th

27 In normal adult gnotobiotic FVB/N animals, network density is on average

28 such colonization by using antimicrobials or gnotobiotic germ-free mice overrides these protective be

29 d IL-2(-/-) mice reared and maintained under gnotobiotic (germfree) conditions.

30 he phylogenetically unassigned NB strain, in gnotobiotic (Gn) calves.

31 We evaluated the gnotobiotic (Gn) pig as a model to study the pathogenesi

32 human rotavirus (HRV) vaccine efficacy in a gnotobiotic (Gn) piglet model of HRV diarrhea.

33 idney cell line, and the WT PEC, passaged in gnotobiotic (Gn) pigs, were used to orally inoculate 13

34 oV-HS66) infects and causes mild diarrhea in gnotobiotic (Gn) pigs.

35 contents of PDCoV OH-FD22 strain-inoculated gnotobiotic (Gn) pigs.

36 pression in buccal and intestinal tissues of gnotobiotic (Gn) pigs; (ii) to determine if virus-like p

37 Both conventionally-raised and gnotobiotic Gpr41-/- mice colonized with the model ferme

38 e degree of gastrointestinal inflammation in gnotobiotic HLA-B27 transgenic rats monoassociated with

39 Utilizing gnotobiotic hosts, we showed that the ID2-dependent earl

40 togenes and orally acquired listeriosis in a gnotobiotic humanized mouse model.

41 study, we demonstrate that immunosuppressed gnotobiotic (IGB) piglets orally inoculated with wild-ty

42 we observed with E. faecalis-induced IBD in gnotobiotic IL-10 KO mice.

43 erichia coli (NC101, which causes colitis in gnotobiotic IL-10(-/-) mice).

44 els of colitis: naive T-cell transfer and in gnotobiotic IL-10(-/-) mice.

45 icantly decreased the DNA damage response in gnotobiotic Il10(-/-) mice.

46 velop GIN and if H pylori accelerates GIN in gnotobiotic INS-GAS mice.

47 onic acid, colitis induced by microflora (in gnotobiotic interleukin-10(-/-)), and colitis induced by

48 sufficient for the organism to colonize the gnotobiotic intestine competitively.

49 Studies of gnotobiotic knockout mice that lack fasting-induced adip

50 howed that bacteria in nonsterile larvae and gnotobiotic larvae inoculated with wild-type E. coli red

51 the underlying mechanisms, we introduce into gnotobiotic mice an artificial community composed of hum

52 study UTI89 recovered from the distal gut of gnotobiotic mice and from IBCs harvested by laser captur

53 tryptophanase, we can modulate IS levels in gnotobiotic mice and in the background of a conventional

54 Moreover, gnotobiotic mice can be used to shape these personalized

55 in vitro during growth in rich medium and in gnotobiotic mice colonized with defined communities of h

56 eneChips to characterize their expression in gnotobiotic mice consuming polysaccharide-rich or -defic

57 We then show MDSINE's utility on two new gnotobiotic mice datasets, investigating infection with

58 gut bacterial strains introduced into adult gnotobiotic mice fed a polysaccharide-rich diet, and (ii

59 matexigens in vitro and in the intestines of gnotobiotic mice harboring a prominent saccharolytic bac

60 We found that gnotobiotic mice harbouring different microbial communit

61 re most obvious in the gut, where studies of gnotobiotic mice have disclosed that the microbiota affe

62 hich human gut communities are replicated in gnotobiotic mice have provided an opportunity to identif

63 Studies in gnotobiotic mice indicate that Methanobrevibacter smithi

64 The humanized gnotobiotic mice mimic humans with a nonsecretor phenoty

65 lethal for adult or neonatal IFN-gamma(-/-) gnotobiotic mice over the 15-week study.

66 analysis of intestinal epithelial cells from gnotobiotic mice revealed a previously unidentified mech

67 ota samples and purified fecal VLPs from the gnotobiotic mice revealed a reproducible nonsimultaneous

68 Pharmacokinetic studies using gnotobiotic mice revealed that dietary protein reduces t

69 nd reuniting them in various combinations in gnotobiotic mice should facilitate preclinical studies d

70 ly higher in the ceca of H. saguini-infected gnotobiotic mice than in the controls.

71 M. smithii's transcriptome and metabolome in gnotobiotic mice that do or do not harbor Bacteroides th

72 file of C. difficile within the intestine of gnotobiotic mice to identify genes regulated in response

73 species, were introduced simultaneously into gnotobiotic mice together with 11 other wild-type strain

74 Using gnotobiotic mice we show that microbiota composition det

75 In this study, gnotobiotic mice were colonized with an artificial micro

76 Gnotobiotic mice were colonized with an assemblage of se

77 epithelial progenitors (GEPs) in transgenic gnotobiotic mice with a ChAG-like phenotype harbor intra

78 Colonization of gnotobiotic mice with a sialidase-deficient mutant of Ba

79 Mono-association of gnotobiotic mice with Bacteroides, a major succinate pro

80 The use of immunodeficient gnotobiotic mice with combined defects in T cells and na

81 erm-free, deliberately colonized adult mice (gnotobiotic mice) were used to examine the efficacy of c

82 ing >90,000 isogenic transposon mutants into gnotobiotic mice, along with the other artificial commun

83 When transplanted into gnotobiotic mice, AMER and CRON microbiota responded pre

84 enced human gut bacteria was introduced into gnotobiotic mice, and changes in species abundance and m

85 odulate serum levels of these metabolites in gnotobiotic mice, and show that in turn this affects int

86 immunocompetent and defined immunodeficient gnotobiotic mice, by reverse-transcription polymerase ch

87 When transplanted into gnotobiotic mice, complete and cultured communities exhi

88 ences in beta -defensin expression in gf and gnotobiotic mice, they also suggest a role for these pep

89 ic pathways, some of which were confirmed in gnotobiotic mice, together with observed changes in the

90 By selectively colonizing gnotobiotic mice, we demonstrate that the allergy-protec

91 l pathology when isolated and transferred to gnotobiotic mice.

92 l controls or defined microbial consortia in gnotobiotic mice.

93 Entry was unaffected in gnotobiotic mice.

94 discordant pairs were each transplanted into gnotobiotic mice.

95 A newly developed gnotobiotic model of intestinal amebiasis should enable

96 n disease, and mechanistic studies employing gnotobiotic model organisms.

97 NMRI mice were colonized at birth from their gnotobiotic mothers, who harbored this anaerobic Gram-ne

98 Using in vitro systems and gnotobiotic mouse colonization models, we find that extr

99 demonstrate that initial colonization of the gnotobiotic mouse intestine by B. fragilis requires that

100 the wild-type strain for colonization of the gnotobiotic mouse intestine.

101 act to regulate gut motility, we developed a gnotobiotic mouse model that mimics short-term dietary c

102 We have used a gnotobiotic mouse model to show that Bacteroides thetaio

103 Using a gnotobiotic mouse model, in which animals were colonized

104 rthermore, the use of genetically engineered gnotobiotic mouse models may increase our understanding

105 Recent studies in gnotobiotic mouse models of ChAG have shown that parieta

106 Using gnotobiotic mouse models, we found that CT induction of

107 BSH enzymes in the gastrointestinal tract of gnotobiotic or conventionally raised mice significantly

108 rus vaccination regimens were evaluated in a gnotobiotic pig model of rotavirus infection and disease

109 This study utilized the neonatal gnotobiotic pig model to evaluate the protective efficac

110 l and systemic T cell responses by using the gnotobiotic pig model.

111 of the attaching and effacing lesion in the gnotobiotic pig.

112 ere constructed and subsequently included in gnotobiotic piglet challenge studies, and their pathogen

113 and subsequently evaluated clinically in the gnotobiotic piglet infection model.

114 h toxin separately and in combination in the gnotobiotic piglet model of CDI.

115 In this study, we used a gnotobiotic piglet model to study determinants of pathog

116 that LT enhanced bacterial colonization in a gnotobiotic piglet model.

117 decrease the incidence of septicemia in the gnotobiotic piglet model.

118 iarrhea but resulting in less mortality in a gnotobiotic piglet-infection model.

119 When administered i.p. to gnotobiotic piglets 6 or 12 h after infection with E. co

120 filaments in the tongues of immunosuppressed gnotobiotic piglets and when embedded in agar, demonstra

121 e virulence of these strains was compared in gnotobiotic piglets expressing receptors for F4(+) fimbr

122 Thirty-six gnotobiotic piglets from six litters were given one of f

123 cted mice challenged with Stx2 and protected gnotobiotic piglets infected with STEC from fatal system

124 Gnotobiotic piglets inoculated with Escherichia coli O15

125 cked-cell volume and plasma total protein of gnotobiotic piglets inoculated with the LT-positive stra

126 Using gnotobiotic piglets orally infected with E. coli O157:H7

127 ions or symptoms developed in 18 (90%) of 20 gnotobiotic piglets orally infected with strain 86-24, i

128 These effects were also documented in gnotobiotic piglets using the same consortium and Malawi

129 Gnotobiotic piglets were colonized with EcN, LGG, or EcN

130 cytotoxin in the pathogenesis of gastritis, gnotobiotic piglets were colonized with either toxigenic

131 Gnotobiotic piglets were used to investigate cross-prote

132 Gnotobiotic piglets were used to study the importance of

133 To investigate this, we challenged gnotobiotic piglets with equal number of oocysts of type

134 owever, in contrast to previous studies with gnotobiotic piglets, there was no evidence that the expr

135 In gnotobiotic piglets, vaccination suppresses but does not

136 culation of 9-day-old F4ac receptor-positive gnotobiotic piglets.

137 ment after the onset of diarrhea in infected gnotobiotic piglets.

138 s evaluated in sheep, conventional pigs, and gnotobiotic piglets.

139 ariant prv 4S, was found to be pathogenic in gnotobiotic piglets.

140 from macaques with AIDS to immunosuppressed gnotobiotic piglets.

141 irHRV) infection and immunity using neonatal gnotobiotic piglets.

142 Gnotobiotic pigs and calves serve as useful models to ev

143 in serum and intestinal contents of neonatal gnotobiotic pigs and IL-12, IFN-gamma, IL-4, and IL-10 c

144 Neonatal gnotobiotic pigs are the only animal model susceptible t

145 n RV (AttHRV) and virulent human RV (HRV) in gnotobiotic pigs colonized with probiotics (Lactobacillu

146 Of 31 neonatal gnotobiotic pigs inoculated with K88ab+ or K88ac+ ETEC,

147 The 2/6-VLPs were administered to gnotobiotic pigs intranasally (i.n.) with a mutant Esche

148 Neonatal gnotobiotic pigs orally inoculated with virulent (intest

149 inoculated pigs was confirmed by inoculating gnotobiotic pigs orally with pooled HRV-positive serum.

150 ptibility to human rotavirus (HRV) diarrhea, gnotobiotic pigs provide a useful model for rotaviral di

151 hese results suggest that GE immunodeficient gnotobiotic pigs serve as a novel model for biomedical r

152 evel serum and mucosal antibody responses in gnotobiotic pigs than those induced by the tissue cultur

153 pe 2 (BoG2) Cryptosporidium parvum, neonatal gnotobiotic pigs were given 1-10 HuG1 or BoG2 oocysts.

154 Four gnotobiotic pigs were inoculated orally with HS206 (6 x

155 Newborn gnotobiotic pigs were inoculated twice perorally (p.o.)

156 Neonatal gnotobiotic pigs were orally inoculated once with virule

157 ate that 2/6-VLP vaccines are immunogenic in gnotobiotic pigs when inoculated i.n. and that the adjuv

158 However, i.n. inoculation of gnotobiotic pigs with 2/6-VLPs did not confer protection

159 HuNoV infects B cells in vivo, we colonized gnotobiotic pigs with E. cloacae and inoculated pigs wit

160 in addition to gastrointestinal infection in gnotobiotic pigs, confirming previous reports of rotavir

161 In neonatal gnotobiotic pigs, the icPC22A-S1Delta197 virus caused mi

162 r respiratory tract infections or viremia in gnotobiotic pigs, we inoculated them with attenuated or

163 lls were not a target cell type for HuNoV in gnotobiotic pigs, with or without E. cloacae colonizatio

164 of an intestinal content fluid filtrate from gnotobiotic pigs.

165 ition of intestinal contents from uninfected gnotobiotic pigs.

166 ty to human rotavirus (HRV) were examined in gnotobiotic pigs.

167 to reduce human rotavirus (HRV) diarrhea in gnotobiotic pigs.

168 this strain and human RV (HRV) Wa G1P[8] in gnotobiotic pigs.

169 Comparison of gnotobiotic Rag1-/- mice with and without subcutaneous 2

170 treptococcus mutans increases virulence in a gnotobiotic rat model and also promotes in vivo accumula

171 stinguishable from its wild-type parent in a gnotobiotic rat model of caries but was significantly le

172 In the gnotobiotic rat model, the gbpA mutant strain was hyperc

173 ish make it an attractive model organism for gnotobiotic research.

174 g gut-resident microorganisms and of rearing gnotobiotic rodents have made it possible to assess the

175 Gnotobiotic studies revealed that while Fusobacterium nu

176 Using gnotobiotic techniques, we show that strains of Klebsiel

177 produced a fitness defect in the stomachs of gnotobiotic transgenic mice but not in wild-type litterm

178 Gnotobiotic transgenic mice with an engineered ablation

179 e cancer-associated strain was less fit in a gnotobiotic transgenic mouse model of human ChAG and bet

180 tropism of H. pylori clinical isolates in a gnotobiotic transgenic mouse model of human chronic atro

181 Previously, we used a gnotobiotic transgenic mouse model with an engineered ab

182 Gnotobiotic transgenic rats raised in Trexler isolators

183 and during 14 months of monocolonization of gnotobiotic wild-type, Rag1-/-, or Myd88-/- mice.

184 Together, these studies establish gnotobiotic zebrafish as a useful model for dissecting t

185 rescent fatty acid (FA) analogs delivered to gnotobiotic zebrafish hosts, we reveal that microbiota s

186 These results demonstrate the utility of gnotobiotic zebrafish in defining the behavior and local

187 To test this assumption, we colonized gnotobiotic zebrafish with zebrafish-derived bacterial i

188 Using the gnotobiotic zebrafish, we discovered that the normal exp

189 Using a gnotobiotic zebrafish-Pseudomonas aeruginosa model, we s

190 e to the commensal microbiota in transparent gnotobiotic zebrafish.

191 e commensal microbiota on gene expression in gnotobiotic zebrafish.