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   1 ers containing peptides such as pardaxin and gramicidin.                                             
     2 those pertaining before the insertion of the gramicidin.                                             
     3 nopore through the support, as recently with gramicidin.                                             
     4  study the folding of the antibiotic peptide gramicidin.                                             
     5 of the peptide to the double-helical form of gramicidin.                                             
     6 orded with the Cl(-)-impermeable pore former gramicidin (25--75 microg ml(-1)) in HCO(3)(-)-free bath
     7 e-iodine and 7 days for neomycin-polymyxin B-gramicidin (95% confidence interval [CI] for difference 
  
  
    10 ts on the transfer of protons in both native gramicidin A (gA) and in covalently linked SS- and RR-di
    11 ces to protons (g(H)) were studied in native gramicidin A (gA) and in the SS and RR diastereoisomers 
    12 channel proton conductances (g(H)) in native gramicidin A (gA) and in two diastereoisomers (SS and RR
  
    14 lar dynamics simulations were performed on a gramicidin A (gA) channel in a fully hydrated dimyristoy
    15 ts of halothane, a clinical anesthetic, on a gramicidin A (gA) channel in a fully hydrated dimyristoy
  
  
    18 mechanism and examined genistein's effect on gramicidin A (gA) channels in planar phospholipid bilaye
  
    20 dipole moment of the four Trp side chains in gramicidin A (gA) channels modify channel conductance th
    21 ferent stereoisomers of the dioxolane-linked gramicidin A (gA) channels reconstituted in planar lipid
    22 ferent stereoisomers of the dioxolane-linked gramicidin A (gA) channels were individually synthesized
    23  single SS stereoisomers of dioxolane-linked gramicidin A (gA) channels were measured in different ph
    24 of the four tryptophans of membrane-spanning gramicidin A (gA) channels, the inclusion of the perpend
  
    26 rried out coarse-grained (CG) simulations of gramicidin A (gA) dimer association and analyzed the res
    27  the three-dimensional stress field around a gramicidin A (gA) dimer in lipid bilayers that feature d
    28 ly kinetics and conformer preferences of the gramicidin A (GA) dimer is investigated using a combinat
    29    The conformational preferences adopted by gramicidin A (GA) dimers inserted into phospholipid bila
    30 ize the volatile anesthetic binding sites in gramicidin A (gA) incorporated into sodium dodecyl sulfa
    31 be the solution phase structure of dimerized Gramicidin A (GA) inserted into lipid vesicle bilayers i
  
  
    34 ata set for homodimeric channels formed from gramicidin A (gA) or any of eight fluorinated Trp analog
    35 as replaced by Ser at position 3 or 5 in the gramicidin A (gA) sequence: formyl-VG(2)A(3)LA(5)VVVWLWL
    36 le ion channel conductance of derivatives of gramicidin A (gA) upon reaction with analytes in solutio
    37 rom labeled tryptophans in membrane-spanning gramicidin A (gA)(1) channels to refine the geometry of 
    38 protons in water wires was studied in native gramicidin A (gA), and in the SS- and RR-diastereoisomer
    39 of measurements of proton conduction through gramicidin A (gA), B (gB), and M (gM) homodimer channels
    40 gallate (nPG)--on bilayer properties using a gramicidin A (gA)-based fluorescence quench assay to pro
  
  
    43 tors incorporate transmembrane peptide pores gramicidin A and alamethicin in the lipid bilayer they c
    44 mer or doubly charged monomer of the peptide gramicidin A and conformers of the [M + 5H](5+) form of 
    45 rent-voltage relations of (5F-Indole)Trp(13) gramicidin A and gramicidin A channels (, 75:2830-2844).
    46 ublished homodimer conductance data for both gramicidin A and gramicidin M channels confirms this con
    47 shable populations about halfway between the gramicidin A and gramicidin M homodimer conductances.   
    48 implies that the principle difference in the gramicidin A and gramicidin M transport free-energy prof
    49 elated to the free-energy difference between gramicidin A and gramicidin M, we construct an effective
    50 (1)H spin diffusion experiments on unlabeled gramicidin A are sufficient to discriminate between the 
  
  
    53 ferent stereoisomers of the dioxolane-linked gramicidin A channel (the SS and RR dimers) were measure
  
    55 rifluorocyclobutane (F3), was found to alter gramicidin A channel function by enhancing Na(+) transpo
  
    57 ifluorocyclobutane causes minimal changes in gramicidin A channel structure in sodium dodecyl sulfate
    58 d closing of the monovalent cation selective gramicidin A channel through single channel conductance,
    59 we consider ion permeation energetics in the gramicidin A channel using a novel polarizable force fie
  
    61 have been proposed for the membrane-spanning gramicidin A channel: one based on solid-state NMR exper
  
  
  
    65 re the consequences of lipid diversity using gramicidin A channels embedded in phosphatidylcholine (P
  
    67 ree energy governing K(+) conduction through gramicidin A channels is characterized by using over 0.1
  
  
  
  
  
  
    74 lular membrane with the liposomes containing gramicidin A forming cation-conductive beta-helix in the
    75 channel formed by a dimer of the polypeptide gramicidin A has a single-stranded, right-handed helical
    76 n of the gramicidin channel, four analogs of gramicidin A have been synthesized in which the tryptoph
    77 ld slower in gramicidin M homodimers than in gramicidin A homodimers and that first- and second-ion e
    78 t claim, the solid-state NMR constraints for gramicidin A in a lipid bilayer are not consistent with 
    79 hree-dimensional continuum elastic model for gramicidin A in a lipid bilayer is shown to describe the
  
    81 m transport study showed that with 75 microM gramicidin A in a vesicle suspension of 66 mM PC/PG, F3 
  
    83 age relations for ion permeation through the gramicidin A ion channel embedded in membranes character
    84 h is utilized to predict current through the Gramicidin A ion channel, a narrow pore in which the app
  
  
  
    88  the conductance of the pore-forming peptide gramicidin A to monitor PLD activity, the work presented
  
    90 k lipid membranes (BLMs) functionalized with gramicidin A were conducted using a fast perfusion syste
  
  
    93  of depths in membrane systems is applied to gramicidin A, a membrane-bound peptide of known structur
    94 using experimental solid-state NMR data from gramicidin A, a monovalent cation channel in lipid bilay
    95 ture of the channels when compared to native gramicidin A, and only small effects are seen on side-ch
  
    97 l as hydrophilic defects and the ion channel gramicidin A, to provide parallels to membranes deformed
    98  a backbone fold identical to that of native gramicidin A, with only small changes in the side chain 
    99 utions were introduced into the enantiomeric gramicidin A-, gA-.) Circular dichroism spectra of [D-Al
   100 Unlike dioleoyl phosphatidylethanolamine and gramicidin A-DOPC, small-angle x-ray scattering and (31)
   101 tions, dioleoyl phosphatidylethanolamine and gramicidin A-DOPC, which form the negatively curved hexa
  
  
  
  
  
  
  
  
  
  
  
  
   114 ced cytotoxicity and direct Na(+) loading by gramicidin-A caused Pico145-resistant cytotoxicity in th
  
   116 ramework model for proton conduction through gramicidin; a model designed to incorporate information 
  
   118  that include formation of a water-insoluble gramicidin aggregate, dissociation from the aggregate, p
  
   120 gAB transcription was shown to be induced by gramicidin and CCCP, agents known to dissipate the proto
  
  
   123 than the known membrane targeting antibiotic gramicidin and the known antifungal agent amphotericin B
   124 e experimental values of bilayer thinning by gramicidin and the shift in the peak position of the in-
   125 icked by treatment with the sodium ionophore gramicidin and were correlated with the increased intrac
   126  agent (sodium arsenite), K-releasing agent (Gramicidin) and a metal ionophore (dithiocarbamate).    
   127 rin, mycosubtilin, nikkomycin, enterobactin, gramicidin, and several proteins from the orphan pksX ge
   128 dies was tested using the model ion channel, gramicidin, and voltage-clamp fluorometry measurements w
  
  
   131 e results indicate that two or more forms of gramicidin are in equilibrium with each other in the lay
   132 enes for the synthesis of antibiotics of the gramicidin/bacitracin family; however, no bacteriophage 
   133 evelop a model for proton conduction through gramicidin based on the molecular dynamics simulations o
  
   135 the integration of GABAergic inputs, we used gramicidin-based patch-clamp recording of STN neurons in
  
  
   138 tion current is consistent with the model of gramicidin being speciated in the monolayer in more than
  
   140 on of the Na(+),K(+)-ATPase or the ionophore gramicidin), cells expressing the D447V mutant rapidly a
   141 en the multiple conformational states of the gramicidin channel and its closed and open states in a l
   142 eptide side chains has been shown to perturb gramicidin channel conductance without significantly cha
  
   144 nt direct measurements of the orientation of gramicidin channel F-Trp positions for use in analysis o
  
   146      This paper reports on a simulation of a gramicidin channel inserted into a fluid phase DMPC bila
  
   148 o individually access each half of a dimeric gramicidin channel makes it possible to generate asymmet
   149 itution on the structure and function of the gramicidin channel, four analogs of gramicidin A have be
  
  
   152 profile in the presence of a high density of gramicidin channels and ran computer simulations of 81 g
  
  
   155  solvation effects of side chain residues of gramicidin channels by double acyl chains and by the pre
  
   157 ollection modes to study K+ transfer through gramicidin channels in a horizontal bilayer lipid membra
  
  
  
   161  channels and ran computer simulations of 81 gramicidin channels to find the equilibrium distribution
   162 probable nearest-neighbor separation between gramicidin channels was 26.8 A in DLPC, but reduced to 2
   163 annels are structurally equivalent to native gramicidin channels, as demonstrated by the formation of
   164 fluorescence self-quenching from dye-labeled gramicidin channels, we observed that the efficiency of 
  
  
  
  
  
  
  
  
   173   The effects of the channel-forming peptide gramicidin D (gD) on the conductance and electroporation
  
  
  
   177    Liquid sample DESI of hydrophobic peptide gramicidin D suggests that the ionization mechanism invo
  
   179 les containing the peptides gramicidin S and gramicidin D were analyzed both with and without the mat
   180 ducer of heat shock and oxidative stress, or gramicidin D, a toxin that selectively permeabilizes cel
  
   182 ed by cell sensitization to Vinca alkaloids, gramicidin D, and Taxol with no effect on cell sensitivi
   183 ble to the polypeptide pore-forming molecule gramicidin D, independent of the Vsa type and length.   
   184 cle fusion to a planar bilayer) to show that gramicidin dimer channels do not normally dissociate whe
   185 agents: sodium arsenite, an oxidative agent; Gramicidin, eliciting K(+) efflux and calcium influx; di
  
   187 ed protein-protein interactions exhibited by gramicidin embedded in dimyristoylphosphatidylcholine (D
   188      In planar bilayers, the Ser-substituted gramicidins form well-defined channels, with cation cond
   189 the feasibility of such a mechanism, we used gramicidin (gA) analogues of different lengths together 
  
  
  
  
   194 with an x-ray crystallographic structure for gramicidin having a double-stranded, right-handed helix 
  
   196 iac glycosides or directly after exposure to gramicidin in low sodium media-is sufficient to disrupt 
   197 -iodine or antibiotics (neomycin-polymyxin B-gramicidin in the Philippines; ciprofloxacin 0.3% in Ind
   198 MR spectroscopy of single-site (15)N-labeled gramicidin in uniformly aligned bilayers in the L(alpha)
   199 pectrometry to study the self-association of gramicidin in various organic and mixed solvents that ar
   200 estigated by synthesizing and characterizing gramicidins in which Trp(9) was ring-labeled and D-Leu(1
   201 annels (e.g., alpha-hemolysin (alpha-HL) and gramicidin) in the bilayer is observed; conversely, reve
   202 nide m-chorophenylhydrazone or the ionophore gramicidin, indicating that the synaptic vesicle proton 
  
   204 h-clamp fluorescence microscopy by measuring gramicidin ion channel conformational changes in a lipid
   205 elastic response provides an explanation for gramicidin ion channel lifetime versus membrane thicknes
   206 nt, the mycoplasmas were highly sensitive to gramicidin irrespective of the length of the Vsa protein
  
  
  
   210 rsion of a complex porin-like channel into a gramicidin-like channel provides a link between two diff
  
   212      A method is demonstrated to extract the gramicidin-lipid boundary condition from all-atom simula
   213 r conductance data for both gramicidin A and gramicidin M channels confirms this conclusion, indicati
  
  
   216 ion step is approximately 100-fold slower in gramicidin M homodimers than in gramicidin A homodimers 
   217 id-state NMR to investigate the structure of gramicidin M in a lipid bilayer and to investigate the m
   218 principle difference in the gramicidin A and gramicidin M transport free-energy profiles occurs at th
   219 e-energy difference between gramicidin A and gramicidin M, we construct an effective ion-Trp free-ene
  
  
   222 he IPSC reversal potential was determined by gramicidin perforated patch recordings to be -65.3 +/- 5
   223 al dentate granule cells were recorded using gramicidin perforated patch techniques at varying times 
  
   225 , we performed cell-attached, whole-cell and gramicidin perforated patch-clamp recordings of progenit
  
  
  
  
  
  
  
  
  
  
  
  
  
   239 m the AOB, and used the whole-cell patch and gramicidin-perforated patch clamp techniques to measure 
  
  
  
  
  
  
   246  both early postnatal and adult periods, and gramicidin-perforated patch-clamp recordings revealed th
  
   248 in embryonic NM neurons using whole-cell and gramicidin-perforated patch-clamp techniques to measure 
  
   250 rded from current-clamped neurones using the gramicidin-perforated technique, the application of taur
  
   252 o inhibited the BK-induced inward current in gramicidin-perforated whole-cell patch-clamp recordings 
   253 corded GABAergic synaptic currents using the gramicidin-perforated-patch method and found their rever
   254 functional group attached to one side of the gramicidin pore induces diodelike conductance behavior i
   255 The transport of a single proton through the gramicidin pore is described by a potential of mean forc
   256 tage of the amplification characteristics of gramicidin pores to sense the activity of picomolar to n
   257 Like previous simulations with a lower lipid/gramicidin ratio, it is found that tryptophan-water hydr
   258 l and beta-helix formation, as in porins and gramicidin, respectively, represent two distinct mechani
   259  previously, dications of the cyclic peptide Gramicidin S (GS) and the photoactive organonometallic c
  
   261 mounts of model peptides HLGLAR (m/z 666.8), gramicidin S (m/z 1142.5), and bovine insulin b chain (m
  
  
   264 osynthesize the symmetric cyclic decapeptide gramicidin S and the cyclic lipoheptapeptide surfactin A
  
  
  
  
  
   270 cyclization activity: the TE domain from the gramicidin S NRPS catalyzes head-to-tail cyclization of 
   271 tion of DPD with viral DNA or the antibiotic gramicidin S resulted in significant biochemical alterat
   272 f the nonribosomal peptide synthetase enzyme gramicidin S synthetase A (GrsA-PheA) for a set of nonco
   273 tion module PheATE (GrsA) of Bacillus brevis gramicidin S synthetase catalyzes the activation, thiola
   274 n (ATE) initiation module of Bacillus brevis gramicidin S synthetase equilibrates the Calpha configur
  
  
   277 clization of a decapeptide thioester to form gramicidin S, and the TE domain from the surfactin NRPS 
  
   279 ly protonated substance P, doubly protonated gramicidin S, doubly protonated neurotensin, and triply 
   280 on also was observed with the cyclic peptide gramicidin S, indicating the generality of the mechanism
   281 n, cephalosporins, streptomycin, fosfomycin, gramicidin S, rapamycin, indolmycin, microcin B17, fumag
  
  
  
  
  
  
  
   289 cules per leaflet were removed to insert the gramicidin, so the resulting preparation had 96 lipid mo
  
   291 ed in redesign for three different proteins: Gramicidin Synthetase A, plastocyanin, and protein G.   
  
   293 ated to the maximum signal after addition of gramicidin, the maximal percent increases in fluorescenc
  
   295 2+) influx or stimulation of Na(+) influx by gramicidin was accompanied by a facilitation of cyclic n
  
  
  
  
  
   301 published data concerning the interaction of gramicidin with bilayers and the hydrophobic mismatch ef
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