ライフサイエンスコーパス: granulysin

1 gens is release of the antimicrobial protein granulysin.

2 dependent on cytotoxic granules that contain granulysin.

3 ectable difference in the levels of Grz A or granulysin.

4 xert specific cytotoxicity via exocytosis of granulysin.

5 echanism for lysis of bacterial membranes by granulysin, a 74-residue basic protein from human cytoly

6 Granulysin, a 9-kDa protein localized to human CTL and N

7 Granulysin, a cationic protein produced by activated hum

8 Granulysin, a molecule expressed by human natural killer

9 Granulysin, a molecule present in the granules of CTL an

10 Granulysin, a peptide stored in the cytoplasmic granules

11 ic cells: a single mRNA gives rise to 15 kDa granulysin, a portion of which is cleaved to a 9 kDa pro

12 Granulysin, a protein found in granules of CTLs, reduced

13 Granulysin, a protein located in the acidic granules of

14 alysis showed that the CD4(+) CTLs expressed granulysin, a recently identified cytolytic molecule ass

15 n, recombinant granulysin and cell-delivered granulysin activate distinct apoptotic pathways in targe

16 in granulysin-treated cells, suggesting that granulysin activates a novel pathway of CTL- and NK cell

17 In this study we show that granulysin also functions as a chemoattractant and activ

18 ed antimycobacterial activity also expressed granulysin, an antimicrobial peptide shown to mediate an

19 not rodent, cytotoxic granules also contain granulysin, an antimicrobial peptide.

20 Moreover, they produced granulysin, an important antimicrobial protein.

21 lation of NKT cells resulted in depletion of granulysin and abrogation of antimycobacterial activity.

22 In addition, recombinant granulysin and cell-delivered granulysin activate distin

23 vation test (LAT) was conducted by measuring granulysin and interferon-gamma to confirm the causaliti

24 Granulysin and NK-lysin are antimicrobial proteins found

25 ponding to the complete functional domain of granulysin and NK-lysin were amplified from bovine PBMC

26 Granulysin and synthetic granulysin-derived peptides pos

27 ells that kill M. tuberculosis organisms via granulysin and the rapid death after infection of beta2

28 molecule transcripts (perforin, granzyme B, granulysin, and Fas ligand) could represent a valuable t

29 reaction (RT-PCR) for perforin, granzyme B, granulysin, and Fas ligand.

30 oexpressed the cytotoxic molecules perforin, granulysin, and granzyme B, which we termed polycytotoxi

31 i.p. stimulates the expression of CD8alpha, granulysin, and IFN-gamma in mucosal and systemic compar

32 d DAP10, and intracellular proteins--SKAP55, granulysin, and NKG7--linked to T cell and NK cell activ

33 Mice expressing transgenic granulysin are better able to clear Listeria monocytogen

34 r localization and functions of 9 and 15 kDa granulysin are largely distinct.

35 iated with higher expression of perforin and granulysin, but not of Fas ligand.

36 initial crystal structure of selenomethionyl granulysin by MAD phasing at 2A resolution.

37 Granulysin causes a 2- to 7-fold increase in chemotaxis

38 by a significantly lower frequency of CD56(+)granulysin(+) cells compared with that in healthy contro

39 The energy of binding granulysin charges to the bacterial membrane could drive

40 Within leprosy lesions, granulysin colocalized in CD4+ T cells and was expressed

41 Despite the reduced granulysin-containing cells in patients with PVOD, GNLY

42 a related protein, NK lysin, suggested that granulysin contains a four alpha helical bundle motif, w

43 data provide evidence that T-cell release of granulysin contributes to host defense in human infectio

44 In this study, we show that granulysin damages cell membranes based upon negative ch

45 Reasoning that granulysin delivered by cytotoxic cells may work in conc

46 Here, we show that granulysin delivers granzymes into bacteria to kill dive

47 of a biologically active bovine homologue to granulysin demonstrates the potential of the bovine mode

48 Granulysin and synthetic granulysin-derived peptides possessing a helix-loop-heli

49 Granulysin directly killed extracellular Mycobacterium t

50 erminus of helix 2 through helix 3 region of granulysin displayed potent antimicrobial activity again

51 The authors report that a granulysin-expressing CD45RA+ subset of effector memory

52 Granulysin-expressing T cells were detected in cutaneous

53 icrobial immunity; and third, association of granulysin expression in natural killer cells with good

54 In previous studies, the t-bet and granulysin genes were upregulated in peripheral blood be

55 Granulysin (GNLY) is a cytolytic molecule expressed by h

56 Granulysin (GNLY) represents a powerful effector protein

57 Granulysin (GNLY), an antimicrobial protein present in t

58 nes, chemokines, or immune alarmins, such as granulysin (GNLY), leading to the extensive tissue damag

59 c granules contain the antimicrobial peptide granulysin (GNLY), which selectively destroys cholestero

60 A murine counterpart to granulysin has not been identified to date, indicating t

61 Studies with recombinant 9 kDa granulysin have demonstrated its cytolytic and proinflam

62 utaredoxin, glutathione peroxidase, NK-lysin/granulysin, HIV Tat protein, H(2)O(2), lipid hydroperoxi

63 sence of protein corresponding to the bovine granulysin homologue in activated T lymphocytes and PBMC

64 Furthermore, we show that NK cells released granulysin in cultures after being stimulated by P. bras

65 uberculosis was dependent on the presence of granulysin in cytotoxic granules, defining a mechanism b

66 , a cytolytic molecule that colocalizes with granulysin in cytotoxic granules, was expressed at simil

67 ascade, interleukins and their receptors and granulysin in graft loss patients compared to patients w

68 We investigated the role of granulysin in human infectious disease using leprosy as

69 e bovine model in characterizing the role of granulysin in the immune response to a variety of infect

70 granzyme A (Grz A), granzyme B (Grz B), and granulysin, in HIV-specific CD8+ T cells from ECs (n = 2

71 Granulysin increased the permeability of bacterial membr

72 While reduction of recombinant 9-kDa granulysin increases lysis of Jurkat cells, reduction of

73 toxin treatment abrogates chemoattraction by granulysin, indicating involvement of G-protein-coupled

74 Granulysin-induced apoptosis is blocked in cells overexp

75 endoplasmic reticulum, prevents a subsequent granulysin-induced increase in [Ca2+](i) in Jurkat cells

76 ride, apamin, and charybdotoxin, inhibit the granulysin-induced increase in intracellular Ca2+ ([Ca2+

77 lular glutathione protects target cells from granulysin-induced lysis, indicating the importance of t

78 Granulysin induces apoptosis of mammalian cells by damag

79 and -9 in targets, cytotoxic cell-delivered granulysin induces endoplasmic reticulum stress and acti

80 rophobic surface that we propose tunnels the granulysin into the fracturing target membrane.

81 Granulysin is a human cytolytic molecule present in cyto

82 ated whether the human antimicrobial protein granulysin is a potential candidate for the treatment of

83 Granulysin is an antimicrobial and tumoricidal molecule

84 Molecular modeling predicts that granulysin is composed of five alpha-helices separated b

85 Nine kilodalton granulysin is confined to cytolytic granules that are di

86 ata indicate that the local concentration of granulysin is critical for the biologic activity, with h

87 Although recombinant 9 kDa granulysin is cytolytic against a variety of tumors and

88 Granulysin is expressed as two isoforms by human cytotox

89 In contrast, 15 kDa granulysin is located in distinct granules that lack per

90 y of tumors and microbes, recombinant 15 kDa granulysin is not.

91 Granulysin is the possible mediator of the cytotoxic eff

92 (cNK-lysin), the chicken homologue of human granulysin, is a cationic amphiphilic antimicrobial pept

93 Thus, the distinct functions of granulysin isoforms have major implications for diagnosi

94 Recombinant 9-kDa granulysin kills a variety of microbes, including bacter

95 nal models to fully characterize the role of granulysin-like molecules in the immune response to infe

96 tides corresponding to the central region of granulysin lyse bacteria, human cells, and synthetic lip

97 Mucosal sequestration of t-bet and granulysin may also explain the presence of inflammatory

98 icating the importance of the redox state in granulysin-mediated cell death.

99 r K+, and elevated extracellular K+ prevents granulysin-mediated cell death.

100 Although granulysin-mediated cytotoxicity occurs at micromolar co

101 l as reactive oxygen species are involved in granulysin-mediated Jurkat cell death.

102 granzyme B (gzmb)-deficient mice to examine granulysin-mediated killing in a more physiologic whole-

103 monocytes requires higher concentrations of granulysin (micromolar).

104 tive charges distribute in a ring around the granulysin molecule, and one face has net positive charg

105 l packing reveals one way to pack a sheet of granulysin molecules at the cell surface for a concerted

106 on, FAS, FAS ligand, perforin, granzyme, and granulysin mRNA expression were associated with intracel

107 ral and functional analogues of NK-lysin and granulysin of porcine and human cytotoxic lymphocytes.

108 n contrast, lysis of bacteria by recombinant granulysin or by cysteine-containing granulysin peptides

109 cl-2 are protected from lysis by recombinant granulysin or the peptides.

110 tructure, function and clinical relevance of granulysin over the past year encompasses three main are

111 Differential activity of granulysin peptides against tumor cells and bacteria may

112 rkat cells, reduction of cysteine-containing granulysin peptides decreases lysis of Jurkat cells.

113 mbinant granulysin or by cysteine-containing granulysin peptides is unaffected by reducing conditions

114 Taken together, these data suggest that granulysin peptides may be useful as topical therapeutic

115 Granulysin peptides were bactericidal, demonstrating an

116 ulated mRNA expression for granzyme A and B, granulysin, perforin, and CD95L (Fas ligand).

117 phocytes and natural killer cells expressing granulysin predominantly infiltrated into the skin lesio

118 At low concentrations (10 nM), granulysin promotes a 3- to 10-fold increase in MCP-1 an

119 Increased perforin and granulysin protein expression was confirmed in both in C

120 rations, presumably further from the site of granulysin release, actively recruit immune cells to sit

121 Cytotoxic cells expressing granulysin require perforin, but not granzyme B, to caus

122 The five-helical bundle of granulysin resembles other "saposin folds" (such as NK-l

123 The ion locations may indicate granulysin's orientation of initial approach towards the

124 nthetic peptides corresponding to the linear granulysin sequence were characterized for lytic activit

125 In vivo, mice expressing granulysin show markedly improved antitumor responses, w

126 The LAT by measuring granulysin showed a sensitivity of 59.3% and specificity

127 T-bet-stained and granulysin-stained cells, MDM and CD138+ plasma cells we

128 Scant granulysin-stained mucosal cells precluded additional ev

129 Our crystal structure of granulysin suggests a mechanism for lysis of bacterial m

130 The presence of bactericidal granulysin suggests these cells may directly mediate hos

131 Given the broad antimicrobial spectrum of granulysin, these data provide evidence that T-cell rele

132 activity and interfered with the ability of granulysin to adhere to Escherichia coli and Mycobacteri

133 These data suggest that the ability of granulysin to kill microbial pathogens is dependent on d

134 in concert with other molecules, we crossed granulysin transgenic (GNLY(+/-)) mice onto perforin (pe

135 In granulysin-treated cells, electron transport is uncouple

136 less, activation of caspase 3 is observed in granulysin-treated cells, suggesting that granulysin act

137 By electron microscopy, granulysin triggered fluid accumulation in the periplasm