ライフサイエンスコーパス: group I ribozyme

1 1 duplex to the remainder of the Tetrahymena group I ribozyme.

2 e N7 atom of residue G264 of the Tetrahymena group I ribozyme.

3 erred to as P1, within the 400nt Tetrahymena group I ribozyme.

4 cture of the P4-P6 domain of the Tetrahymena group I ribozyme.

5 '- and 2'-substrate atoms of the Tetrahymena group I ribozyme.

6 ytic group I introns, the Twort and Azoarcus group I ribozymes.

7 uence context on the activity of therapeutic group I ribozymes.

8 and a particular functional group within the group I ribozyme active site.

9 x recognition surface within the Tetrahymena group I ribozyme active site.

10 ders stabilize the structure of the Azoarcus group I ribozyme, allowing the ribozyme to fold at low p

11 d prior results suggest that the Tetrahymena group I ribozyme, analogous to protein enzymes, uses mul

12 eripheral element stabilizes the Tetrahymena group I ribozyme and enhances its catalytic activity.

13 -resolved scattering experiments on Azoarcus group I ribozyme and single-molecule non-equilibrium per

14 Such rapid folding is unprecedented among group I ribozymes and illustrates the association betwee

15 ia coli, the P4-P6 domain of the Tetrahymena group I ribozyme, and ligand-bound domains from riboswit

16 ead ribozyme, in domain B, the J4/5 motif of group I ribozymes, and connecting the two domains, a "ri

17 a virus, hammerhead, X motif and Tetrahymena group I ribozymes, and various ligand-binding domains.

18 hanges in the size and shape of the Azoarcus group I ribozyme as a function of Mg(2+) and Na(+) conce

19 he folding and helix assembly of a bacterial group I ribozyme at different temperatures and in differ

20 rved secondary structural elements common to group I ribozymes, but lacks several peripheral helices.

21 mutated the major tertiary interactions in a group I ribozyme by single-base substitutions.

22 Here we demonstrate that a trans-splicing group I ribozyme can be employed to intentionally modify

23 Group I ribozymes can repair mutant RNAs via trans-splic

24 The group I ribozyme catalyzes a site-specific attack of gua

25 The Tetrahymena group I ribozyme catalyzes the cleavage of a phosphodies

26 yl group at position A261 of the Tetrahymena group I ribozyme contributes to overall catalysis.

27 reviously found that collapse of a bacterial group I ribozyme correlates with the formation of helice

28 Recent work using the Tetrahymena group I ribozyme demonstrated that CYT-19 possesses a se

29 onment of the active site of the Tetrahymena group I ribozyme (E) using protonated 2'-aminoguanosine

30 in the reaction catalyzed by the Tetrahymena group I ribozyme (E), and the Mn(2+) concentration depen

31 ments on the P4-P6 domain of the Tetrahymena group I ribozyme embedded in Xenopus egg extract demonst

32 into the pre-folded core of the Tetrahymena group I ribozyme exemplifies the formation of tertiary i

33 the two domains, a "ribose zipper", another group I ribozyme feature, formed between the hydroxyl gr

34 ootprinting experiments showed that Azoarcus group I ribozyme forms its tertiary structure rapidly (t

35 helices stabilize a compact state of a small group I ribozyme from Azoarcus pre-tRNA(ile).

36 Recent reports have demonstrated that the group I ribozyme from Tetrahymena thermophila can perfor

37 the trans-splicing reaction catalyzed by the group I ribozyme from Tetrahymenathermophila.

38 w that individual structural elements of the group I ribozyme from the bacterium Azoarcus form sponta

39 gest that structural features inherited from group I ribozymes have undergone speciation due to profo

40 ts of P4-P6 RNA derived from the Tetrahymena group I ribozyme in monovalent and in divalent metal ion

41 port SAXS measurements on a 64 kDa bacterial group I ribozyme in the presence of mono- and divalent i

42 periments are reported on a 64 kDa bacterial group I ribozyme in the presence of polyethylene-glycol

43 Folding of the Tetrahymena group I ribozyme includes sequential accumulation of two

44 The Tetrahymena group I ribozyme is a paradigm for the study of RNA cata

45 Like many structured RNAs, the Tetrahymena group I ribozyme is prone to misfolding.

46 The native structure of the Azoarcus group I ribozyme is stabilized by the cooperative format

47 rtiary domain of the Tetrahymena thermophila group I ribozyme, is known to fold by a secondary struct

48 g-range tertiary contacts of the Tetrahymena group I ribozyme on the dynamics of its substrate helix

49 5'-to-3' order of the structural domains in group I ribozymes optimizes structural communication bet

50 The Tetrahymena group I ribozyme partitions between folding to the nativ

51 e the efficiency with which a trans-splicing group I ribozyme reacts with a targeted RNA in mammalian

52 Binding of the Tetrahymena group I ribozyme's oligonucleotide substrate occurs in t

53 The Tetrahymena group I ribozyme's oligonucleotide substrate, CCCUCUA(5)

54 +-induced folding of Tetrahymena thermophila group I ribozyme shows the capability of the method to s

55 molecules such as PEG stabilize a bacterial group I ribozyme so that the RNA folds in low Mg(2+) con

56 te the development of therapeutically useful group I ribozymes that can repair mutant mRNAs.

57 w that at the active site of the Tetrahymena group I ribozyme the previously identified metal ion int

58 In the Tetrahymena group I ribozyme, the P4, P5, and P6 helices of the core

59 In a two-piece version of the Tetrahymena group I ribozyme, the separated P5abc subdomain folds to

60 conformational steps used by the Tetrahymena group I ribozyme to achieve its active structure and rev

61 demonstrated by the ability of the Azoarcus group I ribozyme to function when its canonical internal

62 ge population of variants of the Tetrahymena group I ribozyme to obtain individuals with a 10(5)-fold

63 Here, we use the group I ribozyme to probe the existence, establishment,

64 lding and unfolding rates of the Tetrahymena group I ribozyme under native conditions.

65 upon addition of 5 mM Mg(2+) to the Azoarcus group I ribozyme up to 80% of chains form compact struct

66 cularly permuted variants of the Tetrahymena group I ribozyme, using time-resolved hydroxyl radical f

67 Our results suggest that group I ribozymes utilize the same interactions with spe

68 olding of P4-P6, a domain of the Tetrahymena group I ribozyme, via single-molecule fluorescence reson

69 a tetraloop with its receptor in a bacterial group I ribozyme was perturbed by site-directed mutagene

70 A trans-splicing group I ribozyme was used to alter mutant beta-globin tr

71 opening of the P1 duplex of the Tetrahymena group I ribozyme were studied by NMR hydrogen exchange e

72 We identified an allosteric group I ribozyme, wherein self-splicing is regulated by

73 Here, a group I ribozyme, which can act as an endoribonuclease,

74 etics of the P4-P6 domain of the Tetrahymena group I ribozyme, which forms a stable, closely packed t