ライフサイエンスコーパス: guanylyltransferase

1 nct from the eukaryotic mRNA capping enzyme, guanylyltransferase.

2 ment in the binding of CaCet1p to the fungal guanylyltransferase.

3 n complex by linkage in cis to the mammalian guanylyltransferase.

4 hat the LEF-4 subunit of RNA polymerase is a guanylyltransferase.

5 enesis of Ceg1, the Saccharomyces cerevisiae guanylyltransferase.

6 ed structure as is formed by protein GTP:RNA guanylyltransferase.

7 from that of eukaryotic mRNA capping enzyme, guanylyltransferase.

8 inactivated the triphosphatase, but not the guanylyltransferase.

9 e capping enzymes RNA triphosphatase and RNA guanylyltransferase.

10 rase and also encodes the RNA capping enzyme guanylyltransferase.

11 iB synthetase, and to AdoCbi-GDP by the CobY guanylyltransferase.

12 h are catalyzed by an RNA triphosphatase and guanylyltransferase.

13 on factor-1alpha, heat-shock protein 60, and guanylyltransferase.

14 tains a submolar amount of cellular mRNA cap guanylyltransferase.

15 nd found that it encodes mannose-1-phosphate guanylyltransferase.

16 in the binding of Schizosaccharomyces pombe guanylyltransferase.

17 hate end that is then capped with GMP by RNA guanylyltransferase.

18 unction in concert with the endogenous yeast guanylyltransferase.

19 ided that it is coexpressed with the S.pombe guanylyltransferase.

20 s with the inherent thermal stability of the guanylyltransferase.

21 d in yeast and vaccinia virus capping enzyme guanylyltransferases.

22 activating mutation (K294A) of the mammalian guanylyltransferase active site in the fusion protein ha

23 es the phosphohydrolase active site from the guanylyltransferase active site.

24 and catalysis at both the triphosphatase and guanylyltransferase active sites.

25 nds on a bifunctional enzyme with kinase and guanylyltransferase activities (CobP in aerobic adenosyl

26 quential action of RNA 5'-triphosphatase and guanylyltransferase activities in the bifunctional mamma

27 ucleoside triphosphate phosphohydrolase, and guanylyltransferase activities of the vaccinia virus mRN

28 Measurement of the relative ATPase and guanylyltransferase activities remaining in D1R carboxyl

29 ossessing ATPase, RNA 5'-triphosphatase, and guanylyltransferase activities, was expressed in Escheri

30 acid protein with RNA triphosphatase and RNA guanylyltransferase activities.

31 proteins with both RNA 5'-triphosphatase and guanylyltransferase activities.

32 bifunctional domain with triphosphatase and guanylyltransferase activities.

33 regard to ATPase, RNA 5'-triphosphatase, and guanylyltransferase activities.

34 mRNA capping enzyme with triphosphatase and guanylyltransferase activities.

35 mRNA capping enzyme with triphosphatase and guanylyltransferase activities.

36 nces the stimulation of human capping enzyme guanylyltransferase activity and RNA cap formation by tr

37 o alanine caused only a partial reduction in guanylyltransferase activity at the autoguanylylation st

38 enzyme had GTP:adenosylcobinamide-phosphate guanylyltransferase activity but did not have the NTP:Ad

39 CTD peptides containing Ser-5-PO4 stimulate guanylyltransferase activity by enhancing enzyme affinit

40 1, but it also allosterically activates Ceg1 guanylyltransferase activity in the context of Pol II bi

41 of the effects of motif VI mutations on Mce1 guanylyltransferase activity in vitro highlights essenti

42 ammalian reovirus particles and contains the guanylyltransferase activity involved in adding 5' caps

43 Here we show that the guanylyltransferase activity of Ceg1 is highly thermolab

44 ith Ceg1 elicits >10-fold stimulation of the guanylyltransferase activity of Ceg1.

45 Cet1p binding to Ceg1p stimulates the guanylyltransferase activity of Ceg1p.

46 sphorylation of CTD by P-TEFb stimulates the guanylyltransferase activity of human capping enzyme and

47 n had no significant effect on the ATPase or guanylyltransferase activity of LEF-4 but resulted in a

48 first inhibitor that targets the GTP-binding/guanylyltransferase activity of the flavivirus RNA cappi

49 king to SAM but had no effects on either the guanylyltransferase activity of this protein or its conf

50 performed with open cores indicates that the guanylyltransferase activity of VP3 is nonspecific and i

51 e Cet1-Ceg1 interaction is to stabilize Ceg1 guanylyltransferase activity rather than to allosterical

52 the guanylyltransferase domain abolished the guanylyltransferase activity without affecting triphosph

53 eterminant of CTD binding and stimulation of guanylyltransferase activity, and of Mce function in viv

54 The LEF-4 subunit has guanylyltransferase activity, suggesting that baculoviru

55 ears to be both necessary and sufficient for guanylyltransferase activity.

56 eptide containing Ser-2-PO4 has no effect on guanylyltransferase activity.

57 ions at these sites retained binding but not guanylyltransferase activity.

58 mRNA capping enzyme (Mce1) and stimulate its guanylyltransferase activity.

59 The N-terminal domain of DENV NS5 has guanylyltransferase and methyltransferase (MTase), and t

60 the rotavirus VP3 enzyme, which encodes both guanylyltransferase and methyltransferase activities, is

61 The fungal guanylyltransferase and methyltransferase are structural

62 additional inserted domain, and a C-terminal guanylyltransferase and RNA 5'-triphosphatase domain.

63 In addition to TbCe1 guanylyltransferase and TbCmt1 (guanine N-7) methyltrans

64 tational studies of Saccharomyces cerevisiae guanylyltransferase and the crystal structures of Chlore

65 hosphorylation-dependent interaction between guanylyltransferase and the CTD is conserved from yeast

66 t has been proposed to function as the viral guanylyltransferase and to direct the capping of the 11

67 Tat stimulates the guanylyltransferase and triphosphatase activities of Mce

68 rs on the structure of murine capping enzyme guanylyltransferase and yeast studies of the recognition

69 the sequential action of RNA triphosphatase, guanylyltransferase, and (guanine-N-7)methyltransferase.

70 on of three enzymes: RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-7)-methyltransfera

71 ctional protein with RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-7-) methyltransfer

72 ast, Schizosaccharomyces pombe and mammalian guanylyltransferases are intrinsically thermostable in v

73 Guanylyltransferases are members of the nucleotidyltrans

74 the separate tunnel-type triphosphatase and guanylyltransferase as the aboriginal state of the cappi

75 Here we localize the guanylyltransferase-binding and guanylyltransferase-stim

76 The guanylyltransferase-binding domain is located on the pro

77 of a WAQKW motif within this segment abolish guanylyltransferase-binding in vitro and Cet1p function

78 We report that mammalian guanylyltransferase binds synthetic CTD peptides contain

79 nding and allosteric activation of mammalian guanylyltransferase by CTD Ser5-PO4, whereas alanine mut

80 This finding illustrates that mammalian guanylyltransferase can be used as a vehicle to deliver

81 lata, we have characterized and purified the guanylyltransferase (capping enzyme), which transfers GM

82 ed by the ceg1-25 mutation of the yeast mRNA guanylyltransferase (capping enzyme).

83 nit contains the signature motifs of GTP:RNA guanylyltransferases (capping enzymes).

84 al and bacterial NLA pathways, two different guanylyltransferases catalyze the activation of the corr

85 l in vivo in yeast in lieu of the endogenous guanylyltransferase Ceg1; (iii) the guanylyltransferase

86 cerevisiae RNA triphosphatase (Cet1) and RNA guanylyltransferase (Ceg1) interact in vivo and in vitro

87 unoblotting suggests that the capping enzyme guanylyltransferase (Ceg1) is stabilized in vivo by its

88 consists of separate triphosphatase (Cet1), guanylyltransferase (Ceg1), and methyltransferase (Abd1)

89 an RNA 5'-triphosphatase (Cet1) and an mRNA guanylyltransferase (Ceg1).

90 he RNA 5'-triphosphatase (Cet1) and the mRNA guanylyltransferase (Ceg1).

91 e mRNA 5'-triphosphatase (Cet1) and the mRNA guanylyltransferase (Ceg1).

92 of the methyltransferase, Abd1, but not the guanylyltransferase, Ceg1, suggesting that Abd1 contribu

93 t1(231-549)p binds in vitro to the yeast RNA guanylyltransferase Ceg1p to form a 7.1 S complex that w

94 erevisiae RNA triphosphatase (Cet1p) and RNA guanylyltransferase (Ceg1p) interact in vivo and in vitr

95 isting of RNA 5'-triphosphatase (Cet1p), RNA guanylyltransferase (Ceg1p), and Abd1p could be replaced

96 endogenous fungal triphosphatase (Cet1p) and guanylyltransferase (Ceg1p).

97 The 2.7 A structure of Candida albicans RNA guanylyltransferase Cgt1 cocrystallized with a carboxy-t

98 Candida albicans guanylyltransferase Cgt1 is also thermolabile and is sta

99 iphosphatase (CaCet1p), a 449-amino acid RNA guanylyltransferase (Cgt1p), and a 474-amino acid RNA (g

100 g bacteria, depends on a bifunctional kinase/guanylyltransferase (CobP) enzyme to convert adenosylcob

101 binamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) from Salmonella typhimurium b

102 binamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) from Salmonella typhimurium h

103 e three-dimensional crystal structure of the guanylyltransferase (CobY) enzyme from the archaeon Meth

104 , we report the crystal structure of the RNA guanylyltransferase component of mammalian capping enzym

105 targeted to pre-mRNAs through binding of the guanylyltransferase component of the capping apparatus t

106 1p homodimerization and Cet1p binding to the guanylyltransferase component of the capping apparatus.

107 iphosphatase and its physical linkage to the guanylyltransferase component of the capping apparatus.

108 us consisting of separate triphosphatase and guanylyltransferase components, which we characterize bi

109 This implies an allosteric change in guanylyltransferase conformation that is specified by th

110 carboxy-terminal half of the C. fasciculata guanylyltransferase containing the six signature motifs

111 Deletion analysis of the Candida guanylyltransferase demarcates an N-terminal domain, Cgt

112 n, Cgt1(1-367)p, suffices for binding to the guanylyltransferase docking site on yeast RNA triphospha

113 ely, mutation of the invariant lysine in the guanylyltransferase domain abolished the guanylyltransfe

114 pping enzyme consisting of an amino-terminal guanylyltransferase domain and a carboxyl-terminal methy

115 nonoverlapping functional domains; (ii) the guanylyltransferase domain Mce1(211-597) is catalyticall

116 Yet, fusion of Cth1p in cis to the guanylyltransferase domain of mammalian capping enzyme a

117 These results demonstrate that the guanylyltransferase domain of mammalian capping enzyme s

118 ed by fusion of D1(1-545)p to the C-terminal guanylyltransferase domain of mammalian capping enzyme,

119 fusion of the mutated triphosphatases to the guanylyltransferase domain of mammalian capping enzyme.

120 re we conducted a mutational analysis of the guanylyltransferase domain of the mouse capping enzyme M

121 49)p is completely restored by fusion to the guanylyltransferase domain of the mouse capping enzyme.

122 dogenous guanylyltransferase Ceg1; (iii) the guanylyltransferase domain per se binds to the phosphory

123 The guanylyltransferase domain was necessary and sufficient

124 id nematode protein consists of a C-terminal guanylyltransferase domain, which is homologous to Ceg1

125 riphosphatase component of LEF-4 but not the guanylyltransferase domain.

126 NTP:AdoCbi kinase (EC 2.7.7.62)/GTP:AdoCbi-P guanylyltransferase (EC 3.1.3.73) and is required for de

127 sisting of separate triphosphatase (EcCet1), guanylyltransferase (EcCeg1), and methyltransferase (Ecm

128 ve tunnel-type triphosphatase and a separate guanylyltransferase encoded by the red alga Cyanidioschy

129 mide kinase/GTP:adenosylcobinamide-phosphate guanylyltransferase enzyme (CobU in Salmonella enterica)

130 hoamide linkage, a characteristic feature of guanylyltransferase enzyme.

131 d physical linkage of the triphosphatase and guanylyltransferase enzymes that catalyze cap formation.

132 al linkage of the RNA triphosphatase and RNA guanylyltransferase enzymes that catalyze mRNA cap forma

133 eparately encoded RNA triphosphatase and RNA guanylyltransferase enzymes.

134 Guanylyltransferase experiments, however, showed that fo

135 7-aa mouse capping enzyme and the C-terminal guanylyltransferase fragment (residues 211-597), unlike

136 s hSPT5 did not increase the activity of the guanylyltransferase fragment.

137 The smaller size of RNA capping guanylyltransferases from other organisms suggested that

138 t functional conservation of eukaryotic mRNA guanylyltransferases from yeast to mammals and indicate

139 inhibition of capping enzyme GTP binding and guanylyltransferase function.

140 NS5 has 5'-RNA methyltransferase (MT)/guanylyltransferase (GT) activities within the N-termina

141 arrying both RNA triphosphatase (RTPase) and guanylyltransferase (GTase) activities.

142 Interactions between RNA guanylyltransferase (GTase) and the C-terminal domain (C

143 ds, we show that the large sub-domain of the guanylyltransferase (GTase) domain of the turret protein

144 The enzyme guanylyltransferase (GTase) plays a central role in the

145 the 5' end of the viral genome and possesses guanylyltransferase, guanine-N7-methyltransferase, and n

146 stood but presumably require triphosphatase, guanylyltransferase, [guanine-N-7]- and [ribose-2'-O]-me

147 Although numerous guanylyltransferases have been identified, studies which

148 ; VP2, the core capsid protein; and VP3, the guanylyltransferase, have affinity for RNA but that only

149 hey express either the S. pombe or mammalian guanylyltransferase in lieu of Ceg1.

150 Stimulation of guanylyltransferase increases with the number of Ser-5-p

151 RNA guanylyltransferase is an essential enzyme that catalyze

152 though no interaction between Cet1 and mouse guanylyltransferase is detectable, both proteins are pre

153 Therefore, the yeast mRNA guanylyltransferase is regulated by allosteric interacti

154 ctose is catalyzed by the octose 1-phosphate guanylyltransferase, LmbO.

155 consisting of a bifunctional triphosphatase-guanylyltransferase Mce1p and the methyltransferase Hcm1

156 TbCgm1 (T. brucei cap guanylyltransferase-methyltransferase) is a novel bifunc

157 s further modified through the activity of a guanylyltransferase, MobA, which converts MoCo to bis-mo

158 binamide kinase/adenosylcobinamide-phosphate guanylyltransferase needed to convert cobinamide to aden

159 reaction indicating that the protein is the guanylyltransferase of the virus.

160 el active-site motif is proposed for the RNA guanylyltransferases of mammalian reoviruses and other R

161 Decreasing either guanylyltransferase or methyltransferase resulted in cas

162 The mRNA guanylyltransferase, or mRNA capping enzyme, cotranscrip

163 A 116-amino acid fragment of the guanylyltransferase Pce1 suffices for binding to the Spt

164 yeast whereby the triphosphatase (Pct1) and guanylyltransferase (Pce1) enzymes of the capping appara

165 e we show that the triphosphatase (Pct1) and guanylyltransferase (Pce1) enzymes of the fission yeast

166 show that P. falciparum encodes separate RNA guanylyltransferase (Pgt1) and RNA triphosphatase (Prt1)

167 gi and Chlorella virus encode monofunctional guanylyltransferase polypeptides that lack triphosphatas

168 ures of Chlorella virus and Candida albicans guanylyltransferases, provide a coherent picture of the

169 The guanylyltransferase reaction has been proposed to procee

170 lambda2 mediates the guanylyltransferase reaction in cap formation and was pr

171 tion of the GTP:adenosylcobinamide-phosphate guanylyltransferase reaction shows the covalent CobU-GMP

172 e ORF YGR024c (THG1) is responsible for this guanylyltransferase reaction.

173 omain which catalyzes the triphosphatase and guanylyltransferase reactions.

174 strains missing either RNA triphosphatase or guanylyltransferase required terminal sequences not pres

175 purified from Escherichia coli catalyzes the guanylyltransferase step of G(-1) addition using a ppp-t

176 g DNA viruses in that the triphosphatase and guanylyltransferase steps of cap formation are catalyzed

177 localize the guanylyltransferase-binding and guanylyltransferase-stimulation functions of Cet1p to a

178 g enzyme Mce1 (a bifunctional triphosphatase-guanylyltransferase) substitutes for Cet1 in vivo.

179 Although the guanylyltransferase subunit can bind alone to the CTD, i

180 03R is more closely related to the yeast RNA guanylyltransferases than it is to the multifunctional c

181 tional studies of the bifunctional bacterial guanylyltransferase that catalyze both ATP-dependent cor

182 osphate of triphosphate-terminated RNA and a guanylyltransferase that reacts with GTP to form a coval

183 To demonstrate that LEF-4 was the guanylyltransferase, the single subunit was overexpresse

184 e activity of the highly conserved tRNA(His) guanylyltransferase (Thg1) enzyme, and no examples of eu

185 The tRNA(His) guanylyltransferase (Thg1) family comprises a set of uni

186 The tRNA(His) guanylyltransferase (Thg1) is a member of a unique enzym

187 The yeast tRNA(His) guanylyltransferase (Thg1) is an essential enzyme in yea

188 tRNA(His) guanylyltransferase (Thg1) post-transcriptionally adds a

189 tRNA(His) guanylyltransferase (Thg1) specifically adds the guanyly

190 and its members include eukaryotic tRNA(His) guanylyltransferase (Thg1), as well as Thg1-like protein

191 addition reaction catalyzed by the tRNA(His) guanylyltransferase (Thg1).

192 Yeast tRNA(His) guanylyltransferase, Thg1, is an essential protein that

193 essential Saccharomyces cerevisiae tRNA(His) guanylyltransferase (Thg1p) is responsible for the unusu

194 inamide ring and subsequently functions as a guanylyltransferase to form adenosylcobinamide.GDP.

195 tiating ribonucleotide triphosphate, we used guanylyltransferase to in vitro cap the replication inte

196 us D1(1-545)p, an RNA triphosphatase and RNA guanylyltransferase-to function in the budding yeast Sac

197 mechanism for mRNA cap formation in that the guanylyltransferase transfers GDP rather than GMP onto t

198 RNA guanylyltransferases typically have a KxDG motif in whic

199 RNA (dsRNA) genome, RNA polymerase VP1, and guanylyltransferase VP3 and are enclosed within a lattic

200 corporation of the polymerase enzyme VP1 and guanylyltransferase VP3 into the core of the virion.

201 Delta cells, thus proving that (i) the viral guanylyltransferase was active in vivo and (ii) the mamm

202 ain of Saccharomyces cerevisiae lacking mRNA guanylyltransferase was complemented for growth by the m

203 with mutations in CEG1, encoding the nuclear guanylyltransferase, were also synthetic lethal with xrn

204 adenosylcobinamide kinase/adenosylcobinamide guanylyltransferase where a P-loop is located at the end

205 PBCV-1 encodes its own mRNA guanylyltransferase, which catalyzes the addition of GMP

206 nsable when Ceg1 is substituted by the mouse guanylyltransferase, which does not require allosteric a

207 :adenosylcobinamide-phosphate [GTP:AdoCbi-P] guanylyltransferase) whose AdoCbi kinase activity is nec

208 D1 subunit that specifically inactivate the guanylyltransferase without affecting the triphosphatase

209 anisms suggested that the lambda2-associated guanylyltransferase would be only a part of this protein