ライフサイエンスコーパス: guard cell

1 lies on quantitative systems analysis of the guard cell.

2 transport across the membrane system of the guard cell.

3 regulation of salicylic acid (SA) pathway in guard cells.

4 dynamics, gas exchange, and ion transport of guard cells.

5 efense and is linked to hormone signaling in guard cells.

6 xpression from bundle sheath to mesophyll to guard cells.

7 ll pores on plant leaves and stems formed by guard cells.

8 idermal cells facilitating ion supply to the guard cells.

9 en by solute accumulation in the surrounding guard cells.

10 nflux and K(+) efflux across the PM of Col-0 guard cells.

11 he understanding of CO2 signaling pathway in guard cells.

12 g several pavement cells adjacent to the two guard cells.

13 a core pathway for CO(2) signalling in plant guard cells.

14 ith its function as an ABA efflux carrier in guard cells.

15 he control of nuclear sizes in trichomes and guard cells.

16 orters results in solute accumulation in the guard cells.

17 anism in ABA and MeJA signal transduction in guard cells.

18 ristics in tobacco epidermis and Arabidopsis guard cells.

19 es in the nuclear size in both trichomes and guard cells.

20 rectifying Kv-like K(+) channel expressed in guard cells.

21 S1 is a unique component of ABA signaling in guard cells.

22 O) in the ABA-dependent signaling network in guard cells.

23 ent of the ABA signaling network in stomatal guard cells.

24 y manipulating sucrose metabolism within the guard cells.

25 to study native ABA responses of Arabidopsis guard cells.

26 ined after ABA treatment in Col but not gpa1 guard cells.

27 n channels and NADPH oxidases in Arabidopsis guard cells.

28 osure by regulating specific ion channels in guard cells.

29 on of NCED3, a key step of ABA synthesis, in guard cells.

30 which is expressed in expanding tissues and guard cells.

31 ced by abscisic acid and highly expressed in guard cells.

32 (C3 plants), bundle-sheath (C4 plants), and guard cells.

33 y a reduced accumulation of K(+) ions in the guard cells.

34 n of signal amplification and specificity in guard cells.

35 rimary and specialized metabolic pathways in guard cells.

36 While DELLA had no effect on ABA levels, guard cell ABA responsiveness was increased in S-della a

37 matal aperture, this occurs by accessing the guard cell ABA signaling pathway.

38 2.6 (SnRK2.6), a protein kinase involved in guard cell ABA signaling, was able to phosphorylate a cy

39 ces stomatal closure via QUAC1/ALMT12 and/or guard cell ABA synthesis.

40 In intact guard cells, abscisic acid (ABA) enhances (primes) the C

41 We measured guard cells across the genera with stomata to assess dev

42 to darkness is mediated by reorganisation of guard cell actin filaments, a process that is finely tun

43 Guard cell actin reorganization has been observed in sto

44 ide anion to oxygen and hydrogen peroxide to guard cells against superoxide toxicity.

45 in the epidermis and surrounded by a pair of guard cells, allow CO2 uptake for photosynthesis and wat

46 A new study makes it clear that guard cells also metabolise starch to accelerate opening

47 l control at the plasma membrane of stomatal guard cells, although stomata of the Arabidopsis syp121

48 Stomatal pores are formed between a pair of guard cells and allow plant uptake of CO2 and water evap

49 ced phospholipid uptake at the root tips and guard cells and are affected in growth and transpiration

50 maximal in the mesophyll compared with both guard cells and bundle sheath.

51 localized synthesis of stilbenes in stomata guard cells and cell walls is induced by P. viticola inf

52 Guard cells and epidermal cells of hornworts show striki

53 coronatine, which blocks the functioning of guard cells and forces stomata to reopen.

54 e is known about redox-sensitive proteins in guard cells and how they function in stomatal signaling.

55 +) currents has not been studied directly in guard cells and it is unknown whether OST1 activity is l

56 expressed mainly in the vascular tissues and guard cells and its expression was strongly up-regulated

57 receptor JAZ2 is constitutively expressed in guard cells and modulates stomatal dynamics during bacte

58 ase gene, PME6, which is highly expressed in guard cells and required for stomatal function.

59 splays impaired osmotic Ca(2+) signalling in guard cells and root cells, and attenuated water transpi

60 K(+) channels of tobacco (Nicotiana tabacum) guard cells and show its close parallel with stomatal cl

61 n involves limited separation between sister guard cells and stomatal responses require reversible gu

62 was observed in the whole stomatal complex (guard cells and subsidiary cells), root vasculature, and

63 establish a link between gene expression in guard cells and their cell wall properties, with a corre

64 ases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and

65 s PEPCK isoform is specifically expressed in guard cells and trichomes of the leaf.

66 l closing and whether starch biosynthesis in guard cells and/or mesophyll cells is rate limiting for

67 lic free Ca(2+) concentration ([Ca(2+)]i) in guard cells, and both processes are facilitated by ABA.

68 rane H(+)-ATPase AHA1 is highly expressed in guard cells, and its activation can induce stomatal open

69 nfirm the expression of CPK13 in Arabidopsis guard cells, and we show that its overexpression inhibit

70 the hormone that leads to the activation of guard cell anion channels by the protein kinase OPEN STO

71 sumption and stress tolerance by controlling guard cell aperture and other protective responses.

72 the molecular basis for circadian control of guard cell aperture, we used large-scale qRT-PCR to comp

73 opening, we have generated SGC (specifically guard cell) Arabidopsis (Arabidopsis thaliana) plants in

74 Although it has long been observed that guard cells are anisotropic due to differential thickeni

75 The focl1 guard cells are larger and less able to reduce the apert

76 Stomatal guard cells are pairs of specialized epidermal cells tha

77 Here, we show that the walls of guard cells are rich in un-esterified pectins.

78 Stomatal guard cells are widely recognized as the premier plant c

79 orms the basis of using the size of stomatal guard cells as a proxy to track changes in plant genome

80 acylglycerols (TAGs), present in Arabidopsis guard cells as lipid droplets (LDs), are involved in lig

81 Starch is present in guard cells at the end of night, unlike in the rest of t

82 e [ADGase]) or retain starch accumulation in guard cells but are starch deficient in mesophyll cells

83 did not, showing that starch biosynthesis in guard cells but not mesophyll functions in CO2-induced s

84 rd type of CPK, CPK13, which is expressed in guard cells but whose role is still unknown.

85 plicated in abscisic acid (ABA) signaling in guard cells, but a metabolite profile of this specialize

86 encoding a flavonol biosynthetic enzyme, in guard cells, but not pavement cells, suggests guard cell

87 The accumulation of flavonol antioxidants in guard cells, but not surrounding pavement cells, was vis

88 channels in both wild-type and syp121 mutant guard cells, but their subsequently recycling was slowed

89 els of flavonols are positively regulated in guard cells by ethylene treatment in the wild type, but

90 diating a massive K(+) efflux in Arabidopsis guard cells by the phosphatase AtPP2CA was investigated.

91 extracellular ATP and of leaf mesophyll and guard cell chloroplasts during light-to-low-intensity bl

92 Quinabactin treatments elicit guard cell closure, suppress water loss, and promote dro

93 These data suggest that guard cell CO2 and ABA signal transduction are not direc

94 bitors and suggest a mechanism through which guard cell CO2 signaling controls plant water management

95 H LEAF TEMPERATURE 1 (HT1)-a central node in guard cell CO2 signaling-and that MPK12 functions as an

96 Guard cells collapse inwardly, increase in surface area,

97 pare circadian oscillator gene expression in guard cells compared with the "average" whole-leaf oscil

98 ts and on the pairwise relationships between guard cell components.

99 This process is mainly regulated by guard cell control of the stomatal aperture, but recent

100 Using a phylogenetic approach to investigate guard cell control, we examined the diversity of stomata

101 Disruption of K(+) accumulation in guard cells correlated with more acidic vacuoles and the

102 The rapidity of gs in dumbbell-shaped guard cells could be attributed to size, whilst in ellip

103 Guard cells determine stomatal aperture and must operate

104 utside inwardly and continues to do so after guard cells die and collapse.

105 tomic force microscopy, that although mature guard cells display a radial gradient of stiffness, this

106 Guard cells dynamically adjust their shape in order to r

107 The stomata, formed by a pair of guard cells, dynamically increase and decrease their vol

108 tants to explore the impact of clustering on guard cell dynamics, gas exchange, and ion transport of

109 ulose and xyloglucan are required for normal guard cell dynamics.

110 ls and stomatal responses require reversible guard cell elongation and contraction.

111 mechanical, pectin-based pinning down of the guard cell ends, which restricts increase of stomatal co

112 nstrated that ABA induces DES1 expression in guard cell-enriched RNA extracts from wild-type Arabidop

113 In silico analysis revealed that guard cells express all the genes required for beta-oxid

114 matal aperture through its inhibition of the guard cell-expressed KAT2 and KAT1 channels.

115 We combine several approaches to identify a guard cell-expressed target.

116 We have characterized FOCL1, a guard cell-expressed, secreted protein with homology to

117 We observed guard cells expressing GFP-tubulin (GFP-TUA6) with confo

118 ntified 390 distinct metabolites in B. napus guard cells, falling into diverse classes.

119 ributed to size, whilst in elliptical-shaped guard cells features other than anatomy were more import

120 closure, with ethylene-induced increases in guard cell flavonols modulating these responses.

121 meeting these challenges and to engineering guard cells for improved water use efficiency and agricu

122 Guard cells form stomatal pores that optimize photosynth

123 appear unchanged at the transcript level in guard cells from C3 and C4 species, but major variations

124 ne Ontology terms previously associated with guard cells from the C3 model Arabidopsis (Arabidopsis t

125 are correlated with and required for normal guard cell function are characterized by changes in micr

126 digesting enzymes, coupled with bioassay of guard cell function) plus modeling lead us to propose th

127 highlight the role of polar reinforcement in guard cell function, which simultaneously improves our u

128 electrophysiological activities required for guard cell function.

129 cal microtubule cytoskeleton is critical for guard cell function.

130 Plant guard cells gate CO2 uptake and transpirational water lo

131 Guard cells (GCs) display transcriptional memory that is

132 ary cells (SCs) flanking two dumbbell-shaped guard cells (GCs)-is linked to improved stomatal physiol

133 cal link between OsGRXS17, the modulation of guard cell H2O2 concentrations, and stomatal closure, ex

134 After the rapid H(+) efflux, the Col-0 guard cells had a longer oscillation period than before

135 pme6-1 mutant guard cells have walls enriched in methyl-esterified pec

136 time-dependent outward potassium currents in guard cells, higher rates of water loss through transpir

137 ]i oscillations and analyze their origins in guard cell homeostasis and membrane transport.

138 tool with which to explore the links between guard cell homeostasis, stomatal dynamics, and foliar tr

139 to plants increased flavonol accumulation in guard cells; however, no flavonol increases were observe

140 nel by the protein kinases OPEN STOMATA1 and GUARD CELL HYDROGEN PEROXIDE-RESISTANT1 (GHR1) in Xenopu

141 ctors can utilize intrinsic HDAC activity to guard cell identity by repressing lineage-inappropriate

142 REDUCTASE (NR)-mediated nitric oxide (NO) in guard cells in an abscisic acid (ABA)-independent manner

143 Here, we characterize transcriptomes from guard cells in C3 Tareneya hassleriana and C4 Gynandrops

144 as physiological characteristics of stomatal guard cells in order to accelerate stomatal movements in

145 ed [Ca(2+)]cyt oscillations in epidermal and guard cells in response to the fungal elicitor chitin.

146 subsidiary cells that occur adjacent to the guard cells in some taxa can be derived either from the

147 Decreased flavonols in guard cells in the anthocyanin reduced (are) mutant and

148 ion across the bundle sheath, mesophyll, and guard cells in the C4 leaf.

149 to the characteristic patterning of stomatal guard cells in the context of a growing leaf.

150 The position of guard cells in the epidermis is ideally suited for cellu

151 d an elevation in H2O2 production within the guard cells, increased sensitivity to ABA, and a reducti

152 The shape of the guard cells influenced the rapidity of response and the

153 e found that irreversible differentiation of guard cells involves RETINOBLASTOMA-RELATED (RBR) recrui

154 d phenotype, suggests that photosynthesis in guard cells is critical for energization and guard cell

155 ledge on CO2 signal transduction in stomatal guard cells is limited.

156 take or release of ions and metabolites from guard cells is necessary to achieve normal stomatal func

157 l responsible for the release of malate from guard cells, is essential for efficient stomatal closure

158 hesis and signalling with K(+) nutrition and guard cell K(+) channel activities have not been fully e

159 ing potassium (K(+) ) nutrition and a robust guard cell K(+) inward channel activity is considered cr

160 e xyloglucan, stomatal apertures, changes in guard cell length, and cellulose reorganization were abe

161 scale investigation into changes in stomatal guard-cell length and use these data to infer changes in

162 ease in ABA biosynthesis specifically in the guard cell lineage.

163 leaves, suggesting that the SA signaling in guard cells may be independent from other cell types.

164 s, promotes rosette expansion, and modulates guard cell mechanics in adult plants.

165 y, CO2, and light, but without connection to guard cell mechanics.

166 Guard cell membrane transport is integral to controlling

167 ly well understood, whereas our knowledge of guard cell metabolism remains limited, despite several d

168 ints to multiple processes and plasticity in guard cell metabolism that enable these cells to functio

169 rther exploring and potentially manipulating guard cell metabolism to improve plant water use and pro

170 Therefore, analysis of guard cell metabolites is fundamental for elucidation of

171 vented stomatal opening and taxol stabilized guard-cell microtubules and delayed stomatal closure.

172 Oryzalin disrupted guard-cell microtubules and prevented stomatal opening a

173 AtERF53 also has a function to regulate guard-cell movement because the stomatal aperture of AtE

174 dy increases while the moderate increases in guard cell nuclear size did not justify for a ploidy inc

175 +) channels at the plasma membrane of intact guard cells of Arabidopsis (Arabidopsis thaliana).

176 Flavonols accumulate in guard cells of Arabidopsis thaliana, but not surrounding

177 Guard cells of are show greater ABA-induced closure than

178 photosynthesis were more highly expressed in guard cells of C4 compared with C3 leaves.

179 quantitative analysis of starch turnover in guard cells of intact leaves during the diurnal cycle.

180 Reductions in LD abundance in guard cells of the lycophyte Selaginella suggest that TA

181 dants, higher levels of ROS were detected in guard cells of the tomato are mutant and lower levels we

182 Guard cells of transparent testa4-2 show more rapid ABA-

183 e detected using a fluorescent ROS sensor in guard cells of transparent testa4-2, which has a null mu

184 rane ion fluxes of H(+) , Ca(2+) and K(+) in guard cells of wild-type (Col-0) Arabidopsis, the CORONA

185 sis plants that are chlorophyll-deficient in guard cells only, expressing a constitutively active chl

186 than the wild type, reduced light-dependent guard cell opening, and reduced water loss, with aw havi

187 ved either from the same cell lineage as the guard cells or from an adjacent cell file.

188 ntiate into one of two epidermal cell types, guard cells or pavement cells.

189 plants where ABA biosynthesis was rescued in guard cells or phloem companion cells of an ABA-deficien

190 sing genetic approaches, we show that ABA in guard cells or their precursors is sufficient to mediate

191 Moreover, the differences in guard cell oscillator function may be important for the

192 misexpressed CCA1 Our results show that the guard cell oscillator is different from the average plan

193 FOCL1-GFP localizes to the guard cell outer cuticular ledge and plants lacking FOCL

194 The regulation of the GORK (Guard Cell Outward Rectifying) Shaker channel mediating

195 ata and developed a biomechanical model of a guard cell pair.

196 uate the current literature on metabolism in guard cells, particularly the roles of starch, sucrose,

197 red stomatal closure requires an increase in guard cell permeability to water and possibly hydrogen p

198 While the function of mesophyll cells, guard cells, phloem companion cells and sieve elements a

199 The role of guard cell photosynthesis in stomatal conductance respon

200 e (from several potential sources; including guard cell photosynthesis) and new evidence that improve

201 e the possible origins of sucrose, including guard cell photosynthesis, and discuss new evidence that

202 l transduction are not directly modulated by guard cell photosynthesis/electron transport.

203 Stomatal guard cells play a key role in gas exchange for photosyn

204 epted that differential radial thickening of guard cells plays an important role in the turgor-driven

205 In this study, Brassica napus guard-cell proteins altered by redox in response to absc

206 Cultured tree tobacco guard cell protoplasts (GCPs) are useful for studying th

207 crease in osmotic water permeability (Pf) of guard cell protoplasts and an accumulation of reactive o

208 analysis utilized approximately 350 million guard cell protoplasts from approximately 30,000 plants

209 -related metabolites in Arabidopsis thaliana guard cell protoplasts over a time course of ABA treatme

210 of PIP2;1 constitutively enhanced the Pf of guard cell protoplasts while suppressing its ABA-depende

211 ic signatures in response to ABA in B. napus guard cell protoplasts.

212 OnGuard software and models of the stomatal guard cell recently developed for exploring stomatal phy

213 chanism underlying CO(2) sensing in stomatal guard cells remains unclear.

214 ins were identified in ABA- and MeJA-treated guard cells, respectively.

215 lls and the plasma membrane of leaf stomatal guard cells, respectively.

216 Imaging cellulose organization in guard cells revealed a relatively uniform distribution o

217 Activation of the guard cell S-type anion channel SLAC1 is important for s

218 they contribute to auxin transport using the guard cell's response as readout of hormone signaling an

219 Stomatal bioassay analyses revealed that guard cell sensitivity to external stimuli, such as absc

220 Guard cells showed fewer microtubule structures as stoma

221 Guard cells shrink and close stomatal pores when air hum

222 e constructed a multi-level dynamic model of guard cell signal transduction during light-induced stom

223 ew functional role of small GTPase, NOG1, in guard cell signaling and early plant defense in response

224 Specially, NOG1-2 regulates guard cell signaling in response to biotic and abiotic s

225 etabolites is fundamental for elucidation of guard cell signaling pathways.

226 posttranslational modifications to fine-tune guard cell signaling.

227 Although the guard-cell-signaling pathway coupling blue light percept

228 e signalling pathways of abiotic stress, but guard cell signalling in response to microbes is a relat

229 t of histidine phosphotransferases (AHPs) in guard cell signalling remain to be fully elucidated.

230 l manipulation will aid our understanding of guard cell signalling.

231 A lack of correlation between guard cell size and DNA content, lack of arabinans in ce

232 ed with trichome branch number increases and guard cell size increases, respectively.

233 Evidence from fossil guard cell size suggests that polyploidy in Sequoia date

234 a constitutively active chlorophyllase in a guard cell specific enhancer trap line.

235 uard cells, but not pavement cells, suggests guard cell-specific flavonoid synthesis.

236 most likely autonomous pools: a constitutive guard cell-specific pool and a facultative environmental

237 Expressing S-della under the control of a guard-cell-specific promoter was sufficient to increase

238 ) was overexpressed under the control of the guard-cell-specific promoter, GC1.

239 Our results show that guard cell starch degradation has an important role in p

240 cs to define the mechanism and regulation of guard cell starch metabolism, showing it to be mediated

241 In guard cells, starch is rapidly mobilized by the synergis

242 onsistent with its known daytime role in the guard cell stroma.

243 , this ion transport was abolished in coi1-1 guard cells, suggesting that MeJA-induced transmembrane

244 Stomatal guard cells surround pores in the epidermis of plant lea

245 olute accumulation by, and its loss from the guard cells surrounding the pore.

246 he regulatory networks and ion fluxes in the guard cells surrounding the stomatal pore [2].

247 Guard cell swelling controls the aperture of stomata, po

248 but not JA-dependent response, is faster in guard cells than in whole leaves, suggesting that the SA

249 across the plasma and vacuolar membranes of guard cells that drive stomatal movements and the signal

250 enhanced disease susceptibility 1 (EDS1), in guard cells that form stomata.

251 Plant gas exchange is regulated by guard cells that form stomatal pores.

252 esponses to [CO2 ] and ABA are functional in guard cells that lack chlorophyll.

253 Each stomate is bordered by a pair of guard cells that shrink in response to drought and the a

254 Plant guard cells, that form stomatal pores for gas exchange,

255 n the epidermal cells of the root tip and in guard cells, the latter of which regulate the size of st

256 Here, we report that, in Arabidopsis guard cells, the tonoplast-localized K(+)/H(+) exchanger

257 other parts of the leaf rather than from the guard cells themselves.

258 transport, metabolism, and signaling of the guard cell to define the water relations and transpirati

259 on changes in turgor pressure acting within guard cells to alter cell shape [1].

260 ) increases reactive oxygen species (ROS) in guard cells to close Arabidopsis (Arabidopsis thaliana)

261 port metabolomic responses of Brassica napus guard cells to elevated CO2 using three hyphenated metab

262 negative regulator of GA signaling, acts in guard cells to promote stomatal closure and reduce water

263 cts directly the molecular properties of the guard cells to their function in the field.

264 We conclude that manipulating guard cell transport and metabolism is just as, if not m

265 physical and kinetic knowledge available for guard cell transport, signaling, and homeostasis.

266 primarily in non-proliferating cells such as guard cells, trichomes, and mesophyll cells, and in vasc

267 tivate the SLAC1 channel, leading to reduced guard cell turgor and stomatal closure.

268 s, and plasma membrane channels that control guard cell turgor pressure.

269 guard cells is critical for energization and guard cell turgor production.

270 Stomatal movements rely on alterations in guard cell turgor.

271 Our results show that guard cell vacuolar accumulation of K(+) is a requiremen

272 w that Wortmannin also induced the fusion of guard cell vacuoles in fava beans, where vacuoles are na

273 ends on changes in osmolyte concentration of guard cell vacuoles, specifically of K(+) and Mal(2-) Ef

274 mones also showed different ABA responses in guard cell versus mesophyll cell metabolomes.

275 The importance of ABA biosynthesis in guard cells versus vasculature for whole-plant stomatal

276 tic solutes that drive reversible changes in guard cell volume and turgor.

277 re governed by osmotically driven changes in guard cell volume, the role of membrane water channels (

278 Combined experimental data (analysis of guard cell wall epitopes and treatment of tissue with ce

279 Hence, PME34 is required for regulating guard cell wall flexibility to mediate the heat response

280 Restoration of PME6 rescues guard cell wall pectin methyl-esterification status, sto

281 function reflects a mechanical change in the guard cell wall.

282 these results provide new insights into how guard cell walls allow stomata to function as responsive

283 ological and genetic analyses to investigate guard cell walls and their relationship to stomatal func

284 xible, but how the structure and dynamics of guard cell walls enable stomatal function remains poorly

285 chanisms for how stomatal pores form and how guard cell walls facilitate dynamic stomatal responses r

286 e signals suggesting that the flexibility of guard cell walls is impaired.

287 at are driven by changes in turgor pressure, guard cell walls must be both strong and flexible, but h

288 , the H(+) efflux and Ca(2+) influx in Col-0 guard cells was impaired by vanadate pre-treatment or PM

289 Our data show that more than 90% of guard cells were chlorophyll-deficient.

290 A total of 358 metabolites from guard cells were quantified in a time-course response to

291 getative plants, BAM1 acts during the day in guard cells, whereas BAM3 is the dominant activity in me

292 ed in actin-dependent nuclear positioning in guard cells, whereas its paralogue SINE2 contributes to

293 Stomata are formed by a pair of guard cells which have thickened, elastic cell walls to

294 bordered by two specialized cells, known as guard cells, which control the stomatal aperture accordi

295 Guard cells, which flank the stomata, undergo adjustment

296 through the development of a new cell type: guard cells, which form stomata.

297 the expression of other transporter genes in guard cells, which ultimately led to improved growth.

298 n accumulation of reactive oxygen species in guard cells, which were both abrogated in pip2;1 plants.

299 division to differentiate highly specialized guard cells while maintaining a stem cell population [1,

300 f stiffness, this is not present in immature guard cells, yet young stomata show a normal opening res