ライフサイエンスコーパス: haemin

1 ique support to integrate molecular catalyst haemin and enzymatic catalyst glucose oxidase for biomim

2 uggesting that it is capable of binding both haemin and haemoglobin and may function as the haemin re

3 diphtheriae and Corynebacterium ulcerans use haemin and haemoglobin as essential sources of iron duri

4 ystem that is involved in the utilization of haemin and haemoglobin as iron sources by a Gram-positiv

5 O157:H7 demonstrated that the utilization of haemin and haemoglobin was ChuA- and TonB-dependent.

6 n of hupC (lmo2429) eliminated the uptake of haemin and haemoglobin, and decreased the virulence of L

7 Both systems can mediate transport of haemin and the siderophores vibriobactin and ferrichrome

8 HmuT binds in vitro to haemin- and haemoglobin-agarose, suggesting that it is c

9 I are both important for S. aureus growth on haemin as a sole iron source and are necessary for full

10 The ability to transport and use haemin as an iron source is frequently observed in clini

11 er TonB system was sufficient for the use of haemin as an iron source, in vitro competition between T

12 n all three haem receptors was unable to use haemin as an iron source.

13 re isolated that allowed E. coli entC to use haemin as an iron source.

14 aroB strain harbouring cloned hmu genes used haemin as both an iron and porphyrin source but only on

15 B1 deletion protein, and no other TonB, used haemin as the iron source in low-osmolarity medium, but

16 ant had only a slight defect in growth using haemin as the iron source, and we show here that V. chol

17 onB1 system mutants were unable to grow with haemin as the sole iron source.

18 completely defective, in the ability to use haemin as the sole iron source.

19 orly on iron-depleted medium containing free haemin as well as mammalian haem-protein complexes inclu

20 Monomeric haemin can be conjugated with graphene through pi-pi int

21 Thus, the integrated graphene-haemin-glucose oxidase catalysts can readily enable the

22 Here we report the design of a graphene-haemin-glucose oxidase conjugate as a tandem catalyst, i

23 hed iron uptake from ferric hydroxamates and haemin/haemoglobin respectively.

24 t the utilization of Fe from citrate (CT) or haemin (HM).

25 ABC transporters for ferric hydroxamates and haemin (Hn)/haemoglobin (Hb) respectively.

26 in low-osmolarity medium, but could not use haemin in high osmolarity.

27 theriae and C. ulcerans mutants defective in haemin iron utilization were isolated and characterized.

28 locus is reduced, and consequently uptake of haemin is impaired in the Ltp1 mutant.

29 e of Yersinia pestis promotes the binding of haemin or Congo red (CR) to the cell surface at temperat

30 emin and haemoglobin and may function as the haemin receptor in C. diphtheriae.

31 Rapid haemin release from the anionic Hb appeared to be a prim

32 with genes that are known to be required for haemin transport in Gram-negative bacteria and are propo

33 with either V. cholerae TonB1 or TonB2, but haemin transport through either receptor was more effici

34 ic space in high osmolarity and thus mediate haemin transport.

35 t functions independently of ChuA to mediate haemin uptake by UPEC strain CFT073.

36 ut only on iron-poor medium, suggesting that haemin uptake is tightly iron regulated.

37 In this study, the haemin uptake locus (hmu) of Y. pestis KIM6+ was selecte

38 absence of Ltp1 transcription across the hmu haemin uptake locus is reduced, and consequently uptake

39 In contrast, haemin uptake through HasR was TonB2 dependent.

40 of this insert was found to be essential for haemin utilization and encoded at least five proteins wi

41 at expression of hma promotes TonB-dependent haemin utilization and the Hma protein binds haemin with

42 urce, but these genes were not essential for haemin utilization in V. cholerae.

43 y complemented several of the Corynebacteria haemin utilization mutants.

44 -126 as a residue necessary for Hma-mediated haemin utilization.

45 s indicates a preferential role for TonB1 in haemin utilization.

46 he presence of hutBCD stimulated growth when haemin was the iron source, but these genes were not ess

47 uA double mutant, which is unable to utilize haemin, was unable to colonize the kidneys to wild-type

48 haemin utilization and the Hma protein binds haemin with high affinity (K(d) = 8 microM).