ライフサイエンスコーパス: haploid

1 aged the therapeutic factor IX cassette into haploid AAV2/8 1:3 capsids and injected them into FIX kn

2 transduction in the liver was observed with haploid AAV2/8 1:3 than that with AAV8 alone.

3 phenotypic correction were achieved with the haploid AAV2/8 1:3 virus vector when compared to that of

4 be further subdivided into 2 subtypes: near-haploid ALL with 24 to 30 chromosomes and low-hypodiploi

5 ice (Oryza sativa L.), which are effectively haploid, allowing easy haplotype construction and imputa

6 que we show that the replication profiles of haploid and diploid cells are indistinguishable, indicat

7 ugh the duration of interphase is similar in haploid and diploid cells, haploid cells spend longer in

8 We find immense diversity of both haploid and diploid gene forms, up to 4.1 and 3.9 millio

9 le with an alternation between multicellular haploid and diploid generations that facilitated efficie

10 the 3D architecture of genomes, constructing haploid and diploid maps of nine cell types.

11 We used haploid and diploid meiotic seed tissues of a single sel

12 phenotype that rapidly drives extinction of haploid and diploid MMR-proficient cells.

13 Bud sites are selected differently in haploid and diploid yeast cells: haploid cells bud in an

14 xamined the performance of screening in both haploid and hypotriploid cell lines, using two alternati

15 sembly projects have been successful in many haploid and inbred species, the assembly of noninbred or

16 Indeed, haploid and repetitive Y chromosomes in species with mal

17 shows that the yeast state of M. osmundae is haploid and the lack of segregation of mating genes sugg

18 ontaneously by auto-diploidization, and both haploids and auto-diploids show a similar reduction in f

19 er, selection acts in opposite directions in haploids and diploids ("ploidally antagonistic selection

20 Therefore, haploids and diploids are both cases of normal euploidy.

21 Compared with haploids and diploids, tetraploids undergo significantly

22 The terms 'haploid' and 'diploid' that describe single (n) and doub

23 Our results apply to a general class of haploid, asexually reproducing, spatially structured pop

24 luated a collection of new de novo long-read haploid assemblies and conclude that although the new as

25 been shown to have similar effects, inducing haploids at high frequencies.

26 e it allows associations to build up between haploid-beneficial alleles and the sex that experiences

27 Using a newly established haploid biofilm model of C. albicans, we found that S. m

28 Cotransfecting dozens of vectors into the haploid blood stages creates complex pools of barcoded m

29 site requires activation of Cdc42 by Bud3 in haploid budding yeast.

30 albicans, we found that S. mutans augmented haploid C. albicans accumulation in mixed-species biofil

31 In Arabidopsis thaliana, haploids can be generated through seeds by crossing a wi

32 We also show that haploids can be used as a tool to accelerate a variety o

33 ferent ratios (3:1, 1:1 and 1:3) to assemble haploid capsids and study both their transduction effici

34 h increasing levels of complexity, including haploid cDNA, haploid genomic DNA and diploid genomic DN

35 The recent development of haploid cell lines has facilitated forward genetic scree

36 and the development of genetic tools such as haploid cell lines, allowing high-throughput screening t

37 tures, telomerase activity becomes limiting: haploid cell populations senesce and generate aneuploid

38 e simulations, colonies initiated by an aged haploid cell show declined mating probability at an earl

39 te asexually within their mammalian hosts as haploid cells and are subject to DNA damage from the imm

40 ormed a genome-wide knockout screen in human haploid cells and identified the calcium pump SPCA1.

41 rting approach also enables the isolation of haploid cells at low percentages, as well as the mainten

42 ferently in haploid and diploid yeast cells: haploid cells bud in an axial manner, while diploid cell

43 Because haploid cells can spontaneously become diploid, their en

44 on is due to a proliferative disadvantage of haploid cells compared with diploid cells.

45 membranes (PSMs), the structures that engulf haploid cells during meiosis II (MII).

46 ious environmental conditions, revealed that haploid cells experienced higher rates of silencing loss

47 We generated loss-of-function human haploid cells for FA complementation group C (FANCC), a

48 ssion of DLGAP4 mRNAs and non-coding RNAs in haploid cells having the translocation.

49 amental genetic feature in mammals, in which haploid cells normally arise only as post-meiotic germ c

50 Meiosis generates haploid cells or spores for sexual reproduction.

51 nstable state, so that cultures of mammalian haploid cells rapidly become enriched in diploids.

52 ase is similar in haploid and diploid cells, haploid cells spend longer in mitosis, indicative of pro

53 In haploid cells, each mutant results in rapid mtDNA deplet

54 plex system for switching the mating type of haploid cells, requiring the genome to have three mating

55 wide insertional mutagenesis screen in human haploid cells.

56 uring fission yeast karyogamy upon mating of haploid cells.

57 the use of forward genetic screens in human haploid cells.

58 establish the physiological role of Ime4 in haploid cells.

59 -activated cell sorting (FACS) enrichment of haploid cells.

60 ) biology that are invisible to contemporary haploid-centric cell biological, proteomic, and function

61 a has been well conserved, yet the number of haploid chromosomes varies widely from 5 to 223.

62 are citrus genomes--a high-quality reference haploid clementine genome and mandarin, pummelo, sweet-o

63 nvestigate whether sex-specific selection on haploids could drive the evolution of recombination supp

64 Haploid cultures of S. rosetta became diploid in respons

65 to correctly handle X-chromosome (and other haploid) data from both males and females.

66 ation, we screened the S. pombe nonessential haploid deletion collection and identified 27 gene delet

67 of 13 meiotic tetrads along with 10 doubled haploids derived from Arabidopsis thaliana hybrids.

68 etes are subsequently regenerated as doubled-haploid (DH) offspring.

69 t with this, a substantial proportion of the haploids die at or shortly after the last mitosis throug

70 Our haploid-diploid eQTL analysis in spruce revealed that co

71 Physcomitrella patens, a haploid dominant plant, is fast becoming a useful molecu

72 Using mating-competent C. albicans haploids, each carrying a different gene drive disabling

73 During female meiosis, haploid eggs are generated from diploid oocytes.

74 nd that the stress treatments used to induce haploid embryo development in culture impinge on this HD

75 abidopsis thaliana, which is recalcitrant to haploid embryo development in culture, also forms embryo

76 Plant breeding and propagation widely use haploid embryo production from in vitro-cultured male ga

77 ature stress that is normally used to induce haploid embryogenesis in B. napus.

78 deacetylases (HDACs) regulate the switch to haploid embryogenesis.

79 Gamete manipulation has yielded haploid embryonic stem (ES) cells from several mammalian

80 Mouse androgenetic haploid embryonic stem cells (AG-haESCs) can support ful

81 Mammalian haploid embryonic stem cells (haESCs) provide new possib

82 ogenous leukemia (KBM7 and HAP1), as well as haploid embryonic stem cells derived from several organi

83 Developmentally incompetent haploid embryos (parthenogenotes) injected with sperm de

84 Decreasing nuclear size in post-MBT haploid embryos caused a further delay in cell cycle len

85 gametophyte is reprogrammed in vitro to form haploid embryos in the absence of exogenous growth regul

86 EPRS-haploid (Eprs(+/-)) mice showed enhanced viremia and inf

87 s well as the maintenance of highly enriched haploid ESC lines throughout passaging.

88 More broadly they exemplify the power of haploid ESCs for genetic interrogation of developmental

89 we develop a forward genetic approach using haploid ESCs.

90 Compared to diploid embryos, haploids exhibited a delay in both zygotic gene expressi

91 y limits imposed on gene expression owing to haploid expression of the X chromosome.

92 tead, the identities of the haploid male and haploid female parents were significant contributors to

93 port that during quiescence, the unicellular haploid fission yeast accumulates mutations as a linear

94 Mice lacking one Pals1 allele (functionally haploid for Pals1) in nephrons developed a fully penetra

95 ly undergoes meiosis to generate recombinant haploid forms.

96 e, the A. thaliana HI can be used to produce haploids from a related species A. suecica and generate

97 ploid states with some organisms existing as haploids (fungi), diploids (most mammals), and polyploid

98 rocess of nuclear fusion is required for two haploid gamete nuclei to form a zygote.

99 mpete for transmission through meiosis, when haploid gametes are created from a diploid parent.

100 res copper to undertake its program in which haploid gametes are produced from diploid precursor cell

101 ganisms manipulate the extent to which their haploid gametes experience selection.

102 rogram by which a diploid cell gives rise to haploid gametes for sexual reproduction.

103 the tsetse vector and involves meiosis, but haploid gametes have not yet been identified.

104 mes to generate daughter cells in mitosis or haploid gametes in meiosis.

105 e and involves the fusion of male and female haploid gametes into a diploid cell.

106 Meiosis produces haploid gametes through a succession of chromosomal even

107 ukaryotic species and involves the fusion of haploid gametes to form a diploid cell that subsequently

108 Meiosis, the mechanism of creating haploid gametes, is a complex cellular process observed

109 enabling the creation of genetically diverse haploid gametes.

110 id cohesion that underlies the production of haploid gametes.

111 ransmission of nonrecombinant chromosomes to haploid gametes.

112 mline, the specialized tissue that generates haploid gametes.

113 n meiosis allow diploid organisms to produce haploid gametes: (1) homologous chromosomes (homologs) p

114 s between two multicellular generations, the haploid gametophyte and the diploid sporophyte [1].

115 ive from the chromosome-doubled cells of the haploid gametophyte.

116 d persist as anther somatic cells mature and haploid gametophytes differentiate into pollen.

117 In bryophytes, haploid gametophytes grow via clonal propagation and pro

118 eiotic chromosome stability and in males for haploid gene transcription during postmeiotic sperm diff

119 Achiasmatic meiosis and haploid generation result in uncommon phenotypes among o

120 represents the hallmark between diploid and haploid generations [1].

121 A genome-wide haploid genetic screen identified the transmembrane prot

122 Using a haploid genetic screen, we identified LDL receptor-relat

123 Haploid genetic screening is a powerful tool to reveal f

124 e CRISPR/Cas9-mediated screens together with haploid genetic screens provide a powerful addition to t

125 Using genome-wide haploid genetic screens, here we identify the lipid-modi

126 To illustrate the power of such haploid genetic screens, we highlight the discovery of t

127 results demonstrate the utility and power of haploid genetics in A. thaliana.

128 e limits of detection (LOD) were less than 5 Haploid Genome Copy (HGC) and the limits of quantificati

129 gnant woman was sequenced to a depth of 270x haploid genome coverage.

130 artificial chromosomes representing 14-fold haploid genome coverage.

131 elegans, occurring in ca. 30 400 copies per haploid genome, averaging ca. 1900 copies per telomere,

132 T oleosin lineage, has one to four genes per haploid genome, only approximately two of which are acti

133 nvestigating false heterozygous calls in the haploid genome, we identified the erroneous realignment

134 In an experiment based on 2184 aligned haploid genomes from the 1000 Genomes Project, our algor

135 Doubled haploid production fixes recombinant haploid genomes in inbred lines, shaving years off the b

136 ression in pollen, with actively transcribed haploid genomes.

137 evels of complexity, including haploid cDNA, haploid genomic DNA and diploid genomic DNA.

138 Using WGS sequences of B73xMo17 doubled haploids, genomic locations showing differential repetit

139 recent evolutionary history of a variety of haploid genotype alignments, as it makes no assumptions

140 proteins and RNA, limiting selection on the haploid genotype.

141 ver, significant selection can also occur on haploid genotypes during less conspicuous life cycle sta

142 mouse spermatogenesis, allowing formation of haploid germ cells that are functional in assisted repro

143 number that deviates from a multiple of the haploid, has been recognized as a common feature of canc

144 Unlike most MA studies that utilize haploid, homozygous, or self-fertilizing lines, D. pulex

145 methylation profiles of three stages of the haploid honey bee genome: unfertilised eggs, the adult d

146 ial for AAV serotype 2 (AAV2) infection in a haploid human cell line.

147 These lines include near-haploid human cell lines isolated from a patient with ch

148 We used extensive mutagenesis in haploid human cells to identify approximately 2000 genes

149 unbiased genome-wide genetic screen in near-haploid human cells to uncover cellular processes that r

150 Deep mutagenesis of haploid human cells was used to identify host factors re

151 and cell sorting, for the identification of haploid human cells within parthenogenetic ESC lines.

152 ed on the use of a forward genetic screen in haploid human cells, followed by a rigorous single and d

153 generated a clonal cell, HAP1-A12, from near-haploid human cells, in which ATP5G1, ATP5G2, and ATP5G3

154 eens versus gene-trap mutagenesis screens in haploid human cells, which represent the existing 'gold

155 c analysis through reporter-based screens in haploid human cells.

156 We have recently derived haploid human embryonic stem cells (ESCs) by parthenogen

157 Haploid human ES cells exhibited typical pluripotent ste

158 S) cells from several mammalian species, but haploid human ES cells have yet to be reported.

159 Although haploid human ES cells resembled their diploid counterpa

160 We expect that haploid human ES cells will provide novel means for stud

161 isolation of essentially pure populations of haploid human ESCs by this protocol requires basic PSC c

162 ic variation, here we sequence and analyse a haploid human genome (CHM1) using single-molecule, real-

163 mappers and five variant callers, both on a haploid human genome and a diploid genome at a similar c

164 Surprisingly, we found that a haploid human genome is compatible not only with the und

165 e, real-time (SMRT) sequencing data from two haploid human genomes.

166 Haploid human pluripotent stem cells (PSCs) integrate ha

167 from whole-genome shotgun reads to model two haploid human satellite arrays on chromosomes X and Y, r

168 We used data generated from a haploid hydatidiform mole genome (CHM1) and a diploid hu

169 ity, we used genomic DNA from an essentially haploid hydatidiform mole, CHM1.

170 o circumvent this process to produce doubled haploid individuals, which derive from the chromosome-do

171 ere we report the development of an improved haploid inducer (HI) strain, SeedGFP-HI, that aids selec

172 lines using intraspecific crosses to in vivo haploid inducer males derived from Stock 6, first report

173 hromosomes are formed from the genome of the haploid inducer, consistent with genomic catastrophes af

174 etic complementation, and gene editing, that haploid induction in maize (Zea mays) is triggered by a

175 , and that novel edits in MTL lead to a 6.7% haploid induction rate (the percentage of haploid progen

176 discovery may enable development of in vivo haploid induction systems to accelerate breeding in crop

177 crops and will have further application for haploid induction to rapidly obtain homozygous lines for

178 f pollen-specific genes overexpressed during haploid induction, some of which may mediate the formati

179 the accelerated tumor onset observed in the haploid-insufficient ErbB2 tumors, deletion of both beta

180 ee major lineages (VNI, VNII, and VNB), some haploid isolates show hybrid ancestry including some tha

181 tenance of human ES cell lines with a normal haploid karyotype.

182 unctional genetic screen in TP53 mutant near-haploid KBM-7 cells using gene-trap insertional mutagene

183 A nonlethal forward genetic screen in near-haploid KBM7 cells identified the HUSH (human silencing

184 d a loss-of-function genetic screen in human haploid KBM7 cells to discover the mechanism of action o

185 ost ERAD components hijacked by US11 in near-haploid KBM7 cells, we identified TMEM129, an uncharacte

186 ltiple times as an adaptation to promote the haploid lifestyle.

187 aining reads in the hydatidiform mole (CHM1, haploid-like) genome.

188 Instead, the identities of the haploid male and haploid female parents were significant

189 ng males with no Y chromosome genes produced haploid male gametes and sired offspring after assisted

190 The haploid male gametophyte, the pollen grain, is a termina

191 Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa,

192 red through hybridization disappear from the haploid males during development from egg to adult as th

193 the building of transgenic colonies via the haploid males.

194 d in diploid females but selected against in haploid males.

195 Accordingly, single-cell-sorted haploid mammalian cells maintain the haploid state for p

196 et was used to enrich and sequence a doubled haploid mapping population of hexaploid wheat derived fr

197 between mating success (number of different haploid mates) and fecundity (number of diploid offsprin

198 d membrane envelopes that package replicated haploid meiotic genomes.

199 s in Saccharomyces cerevisiae, in which four haploid meiotic products become encased by prospore memb

200 Our rich catalogue of haploid methylomes across multiple tissues will allow va

201 rocesses essential to progress from a single haploid microgametocyte to the release of eight flagella

202 Haploid microspores undergo polar nuclear migration and

203 Haploid moss gametophytes harbor distinct stem cell type

204 t HO endonuclease is expressed in late G1 in haploid mother cells to initiate mating-type interconver

205 create a biobank of over 100,000 individual haploid mouse embryonic stem (mES) cell lines targeting

206 stabilizes haploidy in human HAP1 cells and haploid mouse embryonic stem cells.

207 genome provided by sperm coexist as separate haploid nuclei in the zygote.

208 llular stress, polyploid nuclei diminish and haploid nuclei predominate.

209 10), occur at half the diploid level in each haploid nucleus, implying per-chromosome specification o

210 complement that is an exact multiple of the haploid number.

211 Two sections of the genus with haploid numbers >/= 14 have been inferred to be relative

212 induce parthenogenesis and the production of haploid offspring in transgenic sexual pearl millet.

213 rthenogenetic ES cell lines originating from haploid oocytes, leading to the successful isolation and

214 All haploid or diploid eukaryotes studied to date possess a

215 aternal heterozygous SNPs are phased using a haploid PB2 or oocyte as a reference.

216 The occurrence of selection during the haploid phase can have far-reaching consequences for fun

217 tly, with maternal control, selection in the haploid phase either is maximized or reaches an intermed

218 nce of strong purifying selection during the haploid phase of the life cycle and the low level of sex

219 In Ectocarpus, sex is expressed during the haploid phase of the life cycle, and both the female (U)

220 during both the vegetative and reproductive haploid phases along the plant life cycle.

221 asic life cycle with alternating diploid and haploid phases.

222 however, is supported by industrial doubled haploid pipelines using intraspecific crosses to in vivo

223 le triploid populations, because recombinant haploid pollen produced by diploids allows the apomictic

224 sional fertilization of diploid egg cells by haploid pollen, resulting in triploid apomicts that prod

225 that can develop without fertilization, but haploid pollen.

226 y form of epistasis, the total response of a haploid population is proportional to the initial total

227 Doubled haploid production fixes recombinant haploid genomes in

228 7% haploid induction rate (the percentage of haploid progeny versus total progeny).

229 Genetic analysis in haploids provides unconventional yet powerful advantages

230 Si of haploid PV presents cellular infectivity of a single gen

231 NHEJ factor DNA Ligase 4 results in enhanced haploid recovery.

232 obtained a priori to customize the universal haploid reference genome into a personalized diploid ref

233 fies the bioinformatic analysis, as only one haploid reference sample is required to establish phase

234 clear DNA contents showed that PL cells were haploid relative to diploid metacyclics.

235 These results indicate that haploid resolution of long-read sequencing data will sig

236 These translation defects in haploid round spermatids are likely indirect, as neither

237 Using a plasmid-shuffling strategy in haploid Saccharomyces cerevisiae, we observed synthetic

238 We performed a genome-wide haploid screen with the EV-D68 prototype Fermon strain t

239 Iterative haploid screens revealed that the sialyltransferase ST3G

240 , some of which may mediate the formation of haploid seed.

241 ) strain, SeedGFP-HI, that aids selection of haploid seeds prior to germination.

242 s that zygotic sex-ratios become biased when haploid selected loci become linked to the sex-determini

243 Haploid selected loci can favor recombination suppressio

244 tudies needed to refine our understanding of haploid selection among seemingly diploid organisms.

245 aternal-paternal conflict over the extent of haploid selection and describe empirical studies needed

246 atios, we find that a period of sex-specific haploid selection generally favors recombination suppres

247 Although haploid selection is well established in plants, current

248 eficial alleles and the sex that experiences haploid selection most often (e.g., pollen beneficial al

249 In plants, haploid selection should oppose gene loss from Y chromos

250 nistic selection"), mothers evolve to reduce haploid selection to avoid selectively amplifying allele

251 olution of genes that modify the strength of haploid selection to predict when evolution intensifies

252 ld become enriched for genes that experience haploid selection, as is expected for genes that experie

253 These haploid selective processes are typically sex-specific,

254 of gametes instead of traditionally believed haploid selfing in S. sclerotiorum.

255 of the brown alga Ectocarpus sp. that has a haploid sex determination system (UV system) recovering

256 ng event in sexual reproduction, occurs when haploid sperm and egg recognize each other and fuse to f

257 abidopsis (Arabidopsis thaliana) contain two haploid sperm cells enclosed in a haploid vegetative cel

258 iploid mothers to strengthen selection among haploid sperm/pollen, because this reduces the mutation

259 for both male and female fertility, promotes haploid spermatid-specific transcription but has distinc

260 tion is progressively repressed as nuclei of haploid spermatids are compacted through a dramatic chro

261 eby a Lhcgrba-activated signaling cascade in haploid spermatids directs gene expression and the progr

262 These changes are accompanied by a loss of haploid spermatids due to impeded meiosis.

263 h the supporting Sertoli cells, we show that haploid spermatids express the homolog of the tetrapod L

264 mitotic cell division and the production of haploid spermatids from the tetraploid primary spermatoc

265 nal control of mRNA fate in late meiotic and haploid spermatogenic cells.

266 ransformation of stem cells into millions of haploid spermatozoa--is elaborately organized in time an

267 nclude germ-line stem cells, and ending with haploid spermatozoa.

268 l human reproduction is the fusion between a haploid spermatozoon and a metaphase II oocyte.

269 The fusion of the haploid spermatozoon and oocyte is the culminating event

270 aromyces cerevisiae undergo meiosis and form haploid spores, a process collectively referred to as sp

271 them to enter meiosis and differentiate into haploid spores.

272 emical cocktail could maintain haESCs in the haploid state for at least five weeks without fluorescen

273 -sorted haploid mammalian cells maintain the haploid state for prolonged periods, owing to the absenc

274 gression was critical for the maintenance of haploid state.

275 to complete meiosis as demonstrated by their haploid status and the expression of several post-meioti

276 trains is very well studied, but its role in haploid strains has remained unknown.

277 ional validation of plant genes, and propose haploid strategies to reduce the time needed and cost co

278 many crops, while seed-based in vivo doubled haploid systems are rare in nature and difficult to mana

279 ion in a wide range of crop species in which haploid technology is of interest.

280 Here we show that the doubled haploid technology widely used in conventional barley br

281 other, but multiple lineages change from the haploid to the diploid pattern of gene expression.

282 ant, meiosis proceeds efficiently in the two haploid "twin" nuclei, by the same program and timing as

283 r AAV2), with the highest of these being the haploid vector AAV2/8 3:1.

284 ontain two haploid sperm cells enclosed in a haploid vegetative cell.

285 Additionally, the haploid virus AAV2/8 1:3 was able to escape AAV2 neutral

286 To improve the Nab evasion ability of the haploid virus, we produced the triploid vector AAV2/8/9

287 After muscular injection, all of the haploid viruses induced higher transduction than their p

288 ome constructed in silico by merging the two haploids, we find that approximately 59% of the heterozy

289 Haploids were obtained after cenh3 L130F-complemented ce

290 observation that inducer lines do not induce haploids when crossed to themselves.

291 ta=4N e mu for diploids or Theta=2N e mu for haploids (where N e is the effective population size and

292 Unlike land plants, it is a haploid with very few gene duplicates, making it ideal f

293 Interestingly, haploids with increased N/C volume ratios exhibited an i

294 They are haploid, with two alleles present in the population; fre

295 (several versions of the Moran model and the haploid Wright-Fisher model) to examine fixation probabi

296 ns of the Moran process, as well as for the (haploid) Wright Fisher model are presented.

297 Haploid yeast cells secrete mating pheromones that are s

298 Exposure of haploid yeast cells, carrying mating type "a," to "alpha

299 esence of m(6)A-modified FAA1 transcripts in haploid yeast cells.

300 of a reference genome assembly for a double haploid YY male garden asparagus (Asparagus officinalis