ライフサイエンスコーパス: hemicholinium

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1 250 microM) and the choline kinase inhibitor hemicholinium-3 (2 mM) enhanced and inhibited, respectiv

2 lted in a persistent 30-40% reduction of [3H]hemicholinium-3 ([3H]HC-3) binding in the hippocampus th

3 ted with a reduction of ChAT activity (30%), hemicholinium-3 (HC-3) binding (40%), and AChE-positive

4 The hCHT-specific inhibitor hemicholinium-3 (HC-3) blocks choline uptake and choline

5 Hemicholinium-3 (HC-3), an inhibitor of choline kinase,

6 CHT1 is a Na+-dependent, hemicholinium-3 (HC-3)-sensitive choline transporter.

7 CHT1 is a Na(+)- and Cl(-)-dependent, hemicholinium-3 (HC-3)-sensitive, high affinity choline

8 overy was blocked by (-)-vesamicol (VES), by hemicholinium-3 (HC3) and by nicotinic cholinergic agoni

9 take studies were performed with and without hemicholinium-3 (HC3), a selective inhibitor of high aff

10 incipal inhibitors were (-)-vesamicol (VES), hemicholinium-3 (HC3), and NH4+.

11 the reductions of ACh release, vesamicol and hemicholinium-3 also decreased H82 cell proliferation.

12 ited by the structurally similar derivative, hemicholinium-3 and acetylcholine, but not by substrates

13 gated due to inhibition of ACh production by hemicholinium-3 and restored by exogenously added carbac

14 Intracerebroventricular injection of hemicholinium-3 at doses known to deplete the endogenous

15 ne acetyltransferase activity (ChAT) and [3H]hemicholinium-3 binding (HC-3) in the hippocampus, midbr

16 ne acetyltransferase activity (ChAT) and [3H]hemicholinium-3 binding (HC-3) were monitored; ChAT is a

17 sferase, high-affinity choline transport and hemicholinium-3 binding to the choline carrier were redu

18 Hemicholinium-3 binding was reduced in ventral and dorsa

19 activity of the choline transporter and the hemicholinium-3 binding were decreased in all spinal seg

20 nergic markers choline acetyltransferase and hemicholinium-3 binding, a marker for the high-affinity

21 The choline kinase inhibitor hemicholinium-3 inhibits approximately 60% of choline-me

22 s well documented that the sodium dependent, hemicholinium-3 sensitive, high affinity choline co-tran

23 lls, whereas only FCH was inhibited (90%) by hemicholinium-3, a specific inhibitor of choline transpo

24 Vesamicol, an inhibitor of VAChT, and hemicholinium-3, an inhibitor of choline transport, both

25 n preparations stimulated in the presence of hemicholinium-3, an inhibitor of choline uptake, or in N

26 nhibited, when the choline uptake inhibitor, hemicholinium-3, is present or when extracellular Na+ (r

27 y of choline, acquired by a plasma membrane, hemicholinium-3-sensitive (HC-3) choline transporter (CH

28 uptake of choline via the sodium-dependent, hemicholinium-3-sensitive choline transporter (CHT) is b

29 and release is thought to be sustained by a hemicholinium-3-sensitive choline transporter (CHT).

30 Hemicholinium-3-sensitive choline uptake and subsequent

31 dividuals revealed significant reductions in hemicholinium-3-sensitive choline uptake, a finding cons

32 f OCT1 and OCT2 in Xenopus oocytes increased hemicholinium-3-sensitive choline uptake.

33 cultures, we demonstrate that CHO-1 mediates hemicholinium-3-sensitive, high-affinity choline uptake

34 competitive, high-affinity uptake inhibitor, hemicholinium-3.

35 haracterized inhibitor of choline transport, hemicholinium-3.

36 able ACh using inhibitors of choline uptake (hemicholinium-3; 20-50 microM) or vesicular ACh transpor

37 urons, which results in synthesis of ACh, is hemicholinium-sensitive and is referred to as high-affin

38 this procedure, we determined quantitatively hemicholinium-sensitive choline uptake and ChAT enzyme a

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