ライフサイエンスコーパス: hemimethylated

1 ) Compared with the unmethylated duplex, the hemimethylated 20mer specific duplex had a slightly incr

2 poly(dI-dC).poly(dI-dC) and unmethylated and hemimethylated 36- and 75-mer oligonucleotides.

3 SeqA, OxyR and Dam may compete for the same hemimethylated agn43 DNA that is formed after DNA replic

4 Hemimethylated agn43 DNA was also a binding substrate fo

5 OxyR bound hemimethylated agn43 DNA, but the affinity was severalfo

6 f the ON phase, however, Dam must access the hemimethylated agn43 region after DNA replication, and O

7 f the N7 and O6 positions of guanines within hemimethylated and fully methylated CG dinucleotides tow

8 The demethylase targeted both hemimethylated and fully methylated substrates.

9 ak (MB) is a 22-mer oligonucleotide with one hemimethylated and two unmethylated CpG sites, which are

10 ir altered noncognate DNA sequence, and both hemimethylated and unmethylated cognate DNA sequences.

11 Hemimethylated and unmethylated double-stranded DNA show

12 rt crystal structures of MutH complexed with hemimethylated and unmethylated GATC substrates.

13 (Dnmt3a and Dnmt3b) in mouse which methylate hemimethylated and unmethylated templates with equal eff

14 replication fork, the dnaA promoter becomes hemimethylated, and DnaA accumulation drops.

15 n interact differentially with unmethylated, hemimethylated, and homomethylated TIR substrates.

16 thin a CpG context and showed preference for hemimethylated base mismatches.

17 to 5mC-containing DNA in either the fully or hemimethylated CG context or the methylated CHH context

18 n, that shows strong preferential binding to hemimethylated CG sites, the physiological substrate for

19 Furthermore, switching from unmethylated to hemimethylated cognate DNA involves detectable protein c

20 In the hemimethylated complexes, the active site is more compac

21 n IGF2/H19 imprint regulation, including two hemimethylated consensus binding sites for the vertebrat

22 through the ctrA P1 promoter, generating two hemimethylated copies of ctrA.

23 Maintenance methylation of hemimethylated CpG dinucleotides at DNA replication fork

24 ded from the active site to ensure that only hemimethylated CpG dinucleotides undergo methylation.

25 igh specificity the unmethylated strand of a hemimethylated CpG sequence following DNA replication.

26 ssion due to the digestion of SacII when the hemimethylated CpG site is methylated, which inhibits Sa

27 1(731-1602)-DNA complex containing a central hemimethylated CpG site.

28 mouse UHRF1 in complex with DNA containing a hemimethylated CpG site.

29 plication is initiated by the recognition of hemimethylated CpG sites and further flipping of methyla

30 the specificity of DNMT1 for methylation of hemimethylated CpG sites.

31 The DNA is distorted at the hemimethylated CpG step, with side chains from catalytic

32 studies show that UHRF1 binds selectively to hemimethylated CpG via its conserved SRA (SET- and RING

33 the maintenance methyltransferase, and with hemimethylated CpG, the substrate for DNMT1 (refs 1 and

34 s not as high as the rates of methylation of hemimethylated CpGs in otherwise identical oligonucleoti

35 The hemimethylated d(GATC) sequence that directs Escherichia

36 fied M.RSR:I were performed on unmethylated, hemimethylated, dimethylated or non-specific target DNA

37 elucidated by using defined unmethylated or hemimethylated DNA (DNAHM) substrates.

38 NMT1 has been shown to have a preference for hemimethylated DNA and has therefore been termed the mai

39 finity of the enzyme for its two substrates, hemimethylated DNA and S-adenosyl-l-methionine.

40 d upon stoichiometric addition of M.RSR:I to hemimethylated DNA containing the fluorescent analog 2-a

41 the enzyme that is highly active on a 26-bp hemimethylated DNA duplex substrate, the introduction of

42 nd RING finger domains, 1) recruits DNMT1 to hemimethylated DNA during replication and is essential f

43 onsequence of the inefficient replication of hemimethylated DNA in B. anthracis.

44 Since GADD45alpha binds with high avidity to hemimethylated DNA intermediates, it may also provide a

45 ence of its cofactor, and the preference for hemimethylated DNA is increased to 12-fold over unmethyl

46 tosine at CpG sites in fully methylated DNA; hemimethylated DNA is not a significant substrate.

47 The preference of DNMT1 for hemimethylated DNA may be the result of positive coopera

48 suggested that binding cooperativity targets hemimethylated DNA preferentially over unmethylated DNA.

49 li cells lose their ability to interact with hemimethylated DNA sequences of oriC, the chromosomal or

50 ivity; methylase activity can be detected on hemimethylated DNA substrates and residual endonuclease

51 tivity measurements on both unmethylated and hemimethylated DNA substrates.

52 ltransferase and an inactive endonuclease on hemimethylated DNA substrates.

53 ethyltransferase (DNMT1) on unmethylated and hemimethylated DNA templates in order to assess the mech

54 ger affinities of DNA methyltransferases for hemimethylated DNA than for unmethylated or fully methyl

55 -1 (DNMT1) has a higher specific activity on hemimethylated DNA than on unmethylated DNA, but this pr

56 ecular basis for the enzyme's preference for hemimethylated DNA to be the methyl transfer step.

57 suggest that UHRF1 may help recruit DNMT1 to hemimethylated DNA to facilitate faithful maintenance of

58 sence of DNMT1, prolonged binding of NP95 to hemimethylated DNA transiently disrupts SETDB1-dependent

59 In our study, hemimethylated DNA was detected at both origins of V. ch

60 cells these sequences contain high levels of hemimethylated DNA, suggestive of poor maintenance methy

61 In hemimethylated DNA, this motif consists of a 5-methylcyt

62 antly decreased the processivity of DNMT1 on hemimethylated DNA.

63 transferases that recognize newly replicated hemimethylated DNA.

64 ethylated DNA substrates more than 3-fold to hemimethylated DNA.

65 and RING-associated (SRA) domain to bind to hemimethylated DNA.

66 t 501 amino acids retains its preference for hemimethylated DNA.

67 parallel with a higher binding affinity for hemimethylated DNA.

68 n or during homologous recombination between hemimethylated DNA.

69 -1) on unmethylated DNA, and 8.3-49 h(-1) on hemimethylated DNA.

70 The values of k(cat) on hemimethylated DNAs showed a 2-3-fold difference, depend

71 In contrast, hemimethylated double-stranded substrates show burst kin

72 es for single-stranded, double-stranded, and hemimethylated double-stranded substrates.

73 Methylated strands in hemimethylated duplexes were cleaved at a higher rate th

74 ict the relative abundances of unmethylated, hemimethylated, fully methylated, and hydroxymethylated

75 on when it rapidly methylates the adenine in hemimethylated GANTC sequences.

76 mosome (oriC) and the P1 plasmid (P1oriR) at hemimethylated GATC adenine methylation sites.

77 both DNA strands in a discrete patch at each hemimethylated GATC sequence.

78 chia coli SeqA binds clusters of transiently hemimethylated GATC sequences and sequesters the origin

79 ch, the MMR machinery searches for the first hemimethylated GATC site located on its origin-distal si

80 Enzymatic methylation of the hemimethylated GATC site resulted in destabilisation of

81 and extent of mismatch-provoked cleavage at hemimethylated GATC sites by MutH in the presence of Mut

82 eplication origins: different fragments with hemimethylated GATC sites can bind SeqA in vitro when ce

83 esis that PapI-dependent binding of Lrp to a hemimethylated GATC(dist) site generated by DNA replicat

84 ferences in mutation frequency observed when hemimethylated genomes containing PdG on the (-)-strand

85 ce of sinefungin with decreasing affinities: hemimethylated > unmethylated > dimethylated >> non-spec

86 G), incorporated at different positions into hemimethylated (HM) and nonmethylated (NM) DNA duplexes.

87 hing between a methyltransferase function on hemimethylated host DNA and an endonuclease function on

88 DNA, rMcrA-S binds DNA containing a single, hemimethylated HpaII site; however, it does not bind if

89 lly methylated oligonucleotide product and a hemimethylated oligonucleotide substrate using a 13-bp d

90 g poly(dG-dC).poly (dG-dC), unmethylated and hemimethylated oligonucleotides as substrates.

91 DNA methyltransferase were also observed in hemimethylated oligonucleotides, suggesting that this is

92 at methylation spreading can be initiated by hemimethylated or duplex methylated CpGs indicating that

93 lex molecules that were either unmethylated, hemimethylated or fully methylated at CpG sequences and

94 ex deoxyoligonucleotides containing cytosine hemimethylated or fully methylated at N-4 in BslI sites

95 BPDE preferentially modified the guanine in hemimethylated or fully methylated CpG sequences, produc

96 o unmethylated DNA with higher affinity than hemimethylated or methylated DNA.

97 lower affinity for fully methylated than for hemimethylated or unmethylated DNA fragments derived fro

98 ossible because SeqA blocked DnaA binding to hemimethylated oriC only at low-affinity recognition sit

99 The binding of hemimethylated oriC to Escherichia coli membranes has be

100 After initiation, SeqA binds to hemimethylated oriC, sequestering oriC while levels of a

101 re required for the high-affinity binding of hemimethylated oriC.

102 questration by the SeqA protein, which binds hemimethylated origin DNA.

103 Selectivity/preference for hemimethylated over fully methylated DNA may thus reflec

104 Thus, selectivity/preference for hemimethylated over unmethylated DNA appears to result l

105 The preference of the enzyme for hemimethylated, over unmethylated, DNA was 7-21-fold.

106 demonstrated that OxyR is capable of binding hemimethylated P mom , although its affinity is reduced

107 lls can be attributed to its ability to bind hemimethylated P mom DNA, the product of DNA replication

108 te with Dam for assembly on unmethylated and hemimethylated pap DNA.

109 am, Lrp, and the local regulator PapI onto a hemimethylated pap intermediate is a critical step of th

110 lease DpnI, which digests the methylated and hemimethylated parental DNA template.

111 rA gene is preferentially transcribed from a hemimethylated promoter.

112 ith non-palindromic duplex DNA, containing a hemimethylated recognition sequence, and with the cofact

113 In mammals, Dnmt1 is recruited to hemimethylated replication foci by Uhrf1 (Ubiquitin-like

114 DNMT1, either inhibiting the methylation of hemimethylated sites or triggering the inappropriate met

115 The protein appears to recognize individual hemimethylated sites, but must undergo an obligate coope

116 n active demethylase then demethylates these hemimethylated sites.

117 gest that TnpA binds to the postreplicative, hemimethylated Spm sequence and promotes demethylation e

118 ogressively from the fully methylated to the hemimethylated state during DNA replication.

119 n of the ctrA promoter from the fully to the hemimethylated state until late in the cell cycle, inhib

120 ry of a DNA methyltransferase that prefers a hemimethylated substrate, Dnmt1 (4), suggested a mechani

121 mt1 (encoded by Dnmt) shows a preference for hemimethylated substrates in vitro, making the enzyme a

122 Hemimethylated substrates partition toward product forma

123 ibits 3-fold higher catalytic efficiency for hemimethylated substrates.

124 yotes by enzymes that specifically methylate hemimethylated symmetrical sites (e.g., (5')CpGGpC(5') o

125 ne methyltransferase-catalyzed conversion of hemimethylated to fully methylated DNA through a simple,

126 ases, methylated CG sequences, and preferred hemimethylated to unmethylated DNA, as did the full-leng