ライフサイエンスコーパス: hen egg lysozyme

1 odominance and local structural stability in hen egg lysozyme.

2 LPS inhibited processing of bovine RNase and hen egg lysozyme.

3 following exposure of the cells to exogenous hen egg lysozyme.

4 e in model autoantibody systems such as anti-hen egg lysozyme.

5 transgenic CD4 murine cells specific against hen egg lysozyme.

6 o antigen, E/HEL-Tg mice were immunized with hen egg lysozyme.

7 migrated to T cell areas in the response to hen egg lysozyme.

8 xhibit exaggerated tolerance to the model Ag hen-egg lysozyme.

9 In vivo, a low dose of hen egg lysozyme (1 microg) induced significant CD83 but

10 utamic acid decarboxylase 524-543 (self) and hen egg lysozyme 11-23 (foreign) I-A(g7)-binding peptide

11 boxylase (GAD) 206-220 or GAD 524-538 or for hen egg lysozyme 11-25 as a control in NOD, NOD-scid, an

12 In contrast, 3A9 T cells (specific for hen egg lysozyme(46-61)/I-A(k)) are completely eliminate

13 ells, whereas a VLR:Tmu receptor specific to hen egg lysozyme (a self-antigen with respect to chicken

14 The response to hen egg lysozyme, a model antigen with a compact structu

15 ozyme and expressing chronically circulating hen egg lysozyme Ag resulted in induction of high and su

16 pe and CD72-deficient B cells in response to hen egg lysozyme Ag stimulation.

17 mounts of rHEL-C3d3 were more effective than hen egg lysozyme alone in up-regulating c-FLIP levels an

18 ss a chimeric membrane protein consisting of hen egg lysozyme and a hemoglobin epitope tag under the

19 sma VLRB responses of lamprey immunized with hen egg lysozyme and beta-galactosidase (beta-gal), demo

20 Site-specific photocleavage of hen egg lysozyme and bovine serum albumin (BSA) by N-(l-

21 rearranged B cell Ag receptors specific for hen egg lysozyme and expressing chronically circulating

22 protein that binds the acidic active site of hen egg lysozyme and inhibits the enzyme.

23 terminus of beta-catenin, the model proteins hen egg lysozyme and streptavidin, and immunoglobulins f

24 The crystal structure of the complex between hen egg lysozyme and the Fv fragment of a humanized anti

25 were crossed with mice that express soluble hen egg lysozyme and whose B cells bear receptors specif

26 not limited to Smith Ag-specific B cells as hen egg lysozyme- and p-azophenylarsonate-specific B cel

27 mature, respond to antigen, and secrete anti-hen egg lysozyme antibodies.

28 e B10.BR mice transfused with membrane-bound hen egg lysozyme antigen-transgenic RBCs also demonstrat

29 in which naive transgenic cells specific to hen egg lysozyme are adoptively transferred into recipie

30 Equilibrium unfolding of hen egg lysozyme as a function of urea concentration at

31 and timing of exposure to different forms of hen egg lysozyme as a self-Ag.

32 igh and sustained levels of circulating anti-hen egg lysozyme autoantibodies and glomerulonephritis w

33 Anergic hen egg lysozyme autoantigen-binding B cells are also sh

34 by exposing female C57BL/6 wild-type mice or hen egg lysozyme B-cell receptor transgenic mice to desi

35 an anti-ovalbumin T-cell receptor or an anti-hen egg lysozyme B-cell receptor were studied.

36 pansion of Tregs did not occur in BAFF-Tg/Ig hen egg lysozyme BCR chimeras, demonstrating a requireme

37 splenic B lymphocytes from wild-type or anti-hen egg lysozyme BCR transgenic mice, and on a mature mo

38 c models of B cell self-reactivity, the anti-hen egg lysozyme BCR transgenic strain and the AM14 rheu

39 sgenic mice, "edited" B cells that carry non-hen egg lysozyme binding receptors preferentially accumu

40 After immunization with soluble hen egg lysozyme, both MZ and FO B cells captured Ag and

41 vaccinia virus (a smallpox virus surrogate), hen egg lysozyme, cholera toxin, ricin, and staphylococc

42 approached by a bi-specific antibody against hen egg lysozyme consisting of scFv fragments of D1.3 an

43 Finally, by using hen egg lysozyme double transgenic mice, we demonstrated

44 Using the Ig and hen egg lysozyme double transgenic mouse model, we demon

45 Using a hen egg lysozyme double-transgenic model, we further dem

46 s expressed in B cells that are specific for hen egg lysozyme (E/HEL-Tg).

47 3, which flank the immunodominant epitope of hen egg lysozyme (HEL 52-61), were shown to have a profo

48 plex with a naturally processed peptide from hen egg lysozyme (HEL residues 50-62) at 1.9 A resolutio

49 A mouse model utilizing hen egg lysozyme (HEL) "anergic" B cells was studied.

50 Using an mAb (C4H3) specific for the hen egg lysozyme (HEL) 46-61 determinant bound to I-Ak,

51 PFR of the H-2A(k) immunodominant epitope of hen egg lysozyme (HEL) 52-61.

52 active B cells expressing high affinity anti-hen egg lysozyme (HEL) Ag receptors exposed in vivo to m

53 mice that are double-transgenic for soluble hen egg lysozyme (HEL) and an Ig that recognizes HEL wit

54 entation, using a well-characterized antigen hen egg lysozyme (HEL) and comparing it with the closely

55 fic mAb reactive with these structures using hen egg lysozyme (HEL) and I-Ak as a model system.

56 nic (Tg) mice that express a soluble form of hen egg lysozyme (HEL) and in which B cell tolerance to

57 re compared for four antigenic epitopes from hen egg lysozyme (HEL) and RNase.

58 s a transgenic antigen receptor specific for hen egg lysozyme (HEL) and that either lack functional F

59 the association of the 46-61 determinant of hen egg lysozyme (HEL) and the mouse MHC class II molecu

60 in which TCR-transgenic Th cells specific to hen egg lysozyme (HEL) are adoptively transferred to rec

61 4 cells from TCR transgenic mice specific to hen egg lysozyme (HEL) are polarized with IL-6/TGF-beta

62 Cs from transgenic mHEL mice express surface hen egg lysozyme (HEL) as a transmembrane protein.

63 In mice with a diverse B cell repertoire, hen egg lysozyme (HEL) autoantigen-binding B cells are e

64 duction in response to low levels of soluble hen egg lysozyme (HEL) both in vivo and in vitro as dete

65 dy that recognizes peptide residues 48-62 of hen egg lysozyme (HEL) bound to the MHC class II molecul

66 that SIC binds to SLPI and to both human and hen egg lysozyme (HEL) but not to lactoferrin.

67 that B cells, which express BCRs specific to hen egg lysozyme (HEL) display diminished responsiveness

68 shown that fusing complement fragment C3d to hen egg lysozyme (HEL) enhanced the immunogenicity of HE

69 The complex of HyHEL-5 with hen egg lysozyme (HEL) features salt bridges between Fab

70 e observed when crossing STAT5b-CA mice with hen egg lysozyme (HEL) H chain transgenic mice.

71 induced in transgenic (Tg) mice that express hen egg lysozyme (HEL) in their lens, by adoptively tran

72 s genome by inserting the p46-63 sequence of hen egg lysozyme (HEL) into the neuraminidase stalk of t

73 of osmolytes on the association of the anti-hen egg lysozyme (HEL) monoclonal antibody HyHEL-5 with

74 ND CD4+ TCR transgenic mice specific for the hen egg lysozyme (HEL) peptide 48-62:I-Ak and moth cytoc

75 ntitated the major families of peptides from hen egg lysozyme (HEL) presented by MHC class II I-A(k)

76 In vivo experiments in hen egg lysozyme (HEL) T cell receptor transgenic mice s

77 Cs to CpG ODN for 48 h blocked processing of hen egg lysozyme (HEL) to HEL(48-61):I-A(k) complexes.

78 se interactions we imaged B cells (TgB) from hen egg lysozyme (HEL) transgenic mice and DC pulsed wit

79 lysozyme auto-Ag-specific B cells in Ig(Tg) hen egg lysozyme (HEL) transgenic mice inhabit the splee

80 Breeding of the hen egg lysozyme (HEL) transgenic model for B cell anerg

81 o-self-antigen in transgenic mice expressing hen egg lysozyme (HEL) under a retina-specific promoter.

82 the state of tolerance in Tg mice expressing hen egg lysozyme (HEL) under control of the rhodopsin pr

83 ee lines of transgenic (Tg) mice, expressing hen egg lysozyme (HEL) under the control of the alphaA-c

84 express soluble (S-) or membrane-bound (M-) hen egg lysozyme (HEL) under the control of the alphaA-c

85 receptor (TCR) transgenic mice specific for hen egg lysozyme (HEL) were crossed with mice expressing

86 m in which naive CD4 cells, specific against hen egg lysozyme (HEL), are injected into recipient mice

87 immunized with a recombinant model antigen, hen egg lysozyme (HEL), fused to murine C3d.

88 ransgenic (Tg) mice expressing a foreign Ag, hen egg lysozyme (HEL), under control of the alphaA-crys

89 ecipient mice immunized with pDNA encoding a hen egg lysozyme (HEL)-IgFc fusion protein (JwHEL)+JwIL4

90 Persistent cross-linking of hen egg lysozyme (HEL)-specific B cell membrane Ig (mIg)

91 reactive B cells, mice doubly transgenic for hen egg lysozyme (HEL)-specific B cell receptors and sol

92 we compared survival and surface markers of hen egg lysozyme (HEL)-specific B cells in Ig transgenic

93 Using primary B cells that express a hen egg lysozyme (HEL)-specific BCR, we found that oligo

94 isplaying a transgene-encoded BCR that binds hen egg lysozyme (HEL).

95 then challenged intratracheally (i.t.) with hen egg lysozyme (HEL).

96 DN on the differentiation of Th responses to hen egg lysozyme (HEL).

97 s II MHC (MHC-II)-restricted presentation of hen egg lysozyme (HEL).

98 rally processed, I-Ak-restricted peptides of hen egg lysozyme (HEL).

99 rying immunoglobulin transgenes specific for hen egg lysozyme (HEL).

100 the model Ags pigeon cytochrome c (PCC) and hen egg lysozyme (HEL).

101 es to Ags containing disulfide bonds such as hen egg lysozyme (HEL).

102 , which express B cell receptor specific for hen egg lysozyme (HEL).

103 -10 interact with nonoverlapping epitopes on hen egg lysozyme (HEL); the HyHEL-5/HEL interface has tw

104 hibitory gene and an Ig receptor recognizing hen egg lysozyme (HEL-Ig) efficiently escaped developmen

105 proteolytic processing of the model antigen hen-egg lysozyme (HEL).

106 ., exposure of CD4 cells to their target Ag, hen egg lysozyme [HEL] and APCs).

107 ded our findings to another TCR system (anti-hen egg lysozyme [HEL] TCR/HEL mice) where similarly ext

108 usion with RBCs expressing a B-cell epitope (hen egg lysozyme [HEL]) fused to (OVA)(323-339).

109 Allogeneic RBCs expressing the HOD antigen (hen egg lysozyme [HEL]-ovalbumin-human transmembrane Duf

110 ognizing antigens that are (insulin) or not (hen egg lysozyme; HEL) expressed by ss-cells have proven

111 When Clara cell secretory protein-membrane hen egg lysozyme/hemoglobin transgenic mice were crossed

112 Peptide binding studies with variants of the hen egg lysozyme I-Ag7 epitope HEL(11-25) support a comp

113 Here, we demonstrate that anergic B cells in hen egg lysozyme Ig (HEL-Ig)/soluble HEL double transgen

114 Studies in B cell knockout and hen egg lysozyme Ig transgenic mice revealed that B cell

115 since NOD mice immunized with pDNA encoding hen egg lysozyme-IgGFc and IL-4 continued to develop dia

116 nsgenic mice that express a BCR specific for hen egg lysozyme (IgHEL).

117 Hen egg lysozyme immobilized onto polystyrene beads and

118 on (8.4 +/- 1.5 days; n = 18), compared with hen egg lysozyme-immunized C57BL/6 (13.3 +/- 2.2 days; n

119 Using OVA and hen egg lysozyme in crisscross fashion, we confirmed the

120 demonstrated that globular proteins, such as hen egg lysozyme in phosphate buffered saline at room te

121 ely transferred into recipients that express hen egg lysozyme in the lens of the eye.

122 adoptively transferred into mice expressing hen egg lysozyme in their eyes, both Th1 and Th17 induce

123 ar inflammation in recipient mice expressing hen egg lysozyme in their eyes.

124 erance of T cells to the transgenic self Ag, hen egg lysozyme, in a strain with a very low serum lyso

125 CR-transgenic Th1 or Th17 cells specific for hen egg lysozyme induce ocular inflammation in recipient

126 polarized Th1 or Th17 cells specific against hen egg lysozyme induce ocular inflammation in recipient

127 ch during B cell ontogeny, autoreactive anti-hen egg lysozyme MD4 Ig transgene B cells are negatively

128 ion, we have studied the development of anti-hen egg lysozyme MD4 Ig transgene B cells while systemat

129 erated selectively expressing membrane-bound hen egg lysozyme (mHEL) on the thyroid epithelium.

130 deamidation of the HEL(48-62) peptide in the hen egg lysozyme model of autoimmunity.

131 ve was incubated with excess protein ligand (hen egg lysozyme or bovine insulin) at 23, 37, or 50 deg

132 ss of stability was accompanied by a loss in hen egg lysozyme or hsp70 peptide-binding ability.

133 ells stimulated via the BCR with the antigen hen egg lysozyme, or surrogate for antigen anti-IgM, dem

134 on, neonatal injection of BALB/c mice with a hen egg lysozyme peptide 106-116 in putative "tolergenic

135 ope after immunization with several doses of hen egg lysozyme protein.

136 The main peptides examined were hen egg lysozyme residues 48-62 and heat shock protein (

137 expressing either soluble or membrane-bound hen egg lysozyme (sHEL or mHEL, respectively) under cont

138 mice expressing a model autoantigen (soluble hen egg lysozyme, sHEL) and high-affinity HEL-specific I

139 e tandemly arranged copies of C3d and the Ag hen egg lysozyme, shown to be a highly effective immunog

140 To address this question, we used hen egg lysozyme-specific BCR transgenic mice to elucida

141 otoreceptor retinoid-binding promoter, and a hen egg lysozyme-specific CD4(+) TCR transgene.

142 promised neither the formation of functional hen egg lysozyme-specific IgM nor the secretion of free

143 pecific to a different Ag, we tested whether hen egg lysozyme-specific Th1 cells could synergize with

144 at display a cell surface Ag, membrane-bound hen egg lysozyme, strongly activate Ag-specific B cells.

145 e-regulated expression of a secreted form of hen egg lysozyme, tagged with a murine hemoglobin (Hb) e

146 model, we took a segment of polypeptide from hen egg lysozyme that in the native protein forms the bi

147 f principle, we demonstrate this method with hen egg lysozyme that shows at least two kinetic phases

148 Using the hen egg lysozyme transgenic system, we show that IgM(hig

149 ared to 2/26 of saline-treated or to 1/10 of hen egg lysozyme-treated control mice (P < 0.01).

150 generated double-transgenic mice expressing hen egg lysozyme, under the retinal interphotoreceptor r

151 To address these questions, a model Ag, hen egg lysozyme, was targeted to various subcellular co

152 in recipient mice expressing eye-restricted hen egg lysozyme, we found important differences in the

153 trast, two other antigens, phycoerythrin and hen egg lysozyme, were not captured by these cells.

154 he pH-dependent energetics of association of hen egg lysozyme with two closely related monoclonal ant

155 an airway challenge with the soluble antigen hen egg lysozyme yields rapid acquisition of specific an