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2 Cs were performed with cow's milk (n = 633), hen's egg (n = 456), wheat (n = 265) and/or soy (n = 317
4 e for peanut (n = 135), cow's milk (n = 93), hen's egg (n = 53), hazelnut (n = 28), and cashew nut (n
6 ecipient mice immunized with pDNA encoding a hen egg lysozyme (HEL)-IgFc fusion protein (JwHEL)+JwIL4
8 models, one in which T cells specific for a hen-egg white lysozyme (HEL) peptide were injected into
11 transfected with I-Ak genes and the model Ag hen egg white lysozyme targeted to the endoplasmic retic
13 approached by a bi-specific antibody against hen egg lysozyme consisting of scFv fragments of D1.3 an
14 -old infants who were not sensitized against hen's egg, as determined based on specific serum antibod
15 m in which naive CD4 cells, specific against hen egg lysozyme (HEL), are injected into recipient mice
16 polarized Th1 or Th17 cells specific against hen egg lysozyme induce ocular inflammation in recipient
19 tering of solutions of bovine serum albumin, hen egg white ovalbumin, hen egg white ovomucoid, and bi
20 ude of fertilized eggs laid by high altitude hens also restricted fetal growth, but to a lesser exten
21 vel of fertilized eggs laid by high altitude hens not only restored, but enhanced, fetal growth relat
26 ast to family members (human lactoferrin and hen ovotransferrin), both lobes are almost equally open:
32 conserved across lytic transglycosylases and hen egg white lysozyme, and this differentiating asparta
34 serotype Kentucky received 10 exp9 CFU, and hens injected with serotype Enteritidis received 10 exp7
35 eople (>30,000 cases of human paralysis) and hens (the best animal model for this demyelinating disea
37 active B cells expressing high affinity anti-hen egg lysozyme (HEL) Ag receptors exposed in vivo to m
39 ch during B cell ontogeny, autoreactive anti-hen egg lysozyme MD4 Ig transgene B cells are negatively
40 ion, we have studied the development of anti-hen egg lysozyme MD4 Ig transgene B cells while systemat
41 ived from an affinity-matured series of anti-hen egg white lysozyme (HEL) mouse IgG1, were constructe
42 splenic B lymphocytes from wild-type or anti-hen egg lysozyme BCR transgenic mice, and on a mature mo
44 ded our findings to another TCR system (anti-hen egg lysozyme [HEL] TCR/HEL mice) where similarly ext
45 c models of B cell self-reactivity, the anti-hen egg lysozyme BCR transgenic strain and the AM14 rheu
46 characterize the interface between the anti-hen egg white lysozyme (HEL) antibody HyHEL-63 and HEL.
47 ells stimulated via the BCR with the antigen hen egg lysozyme, or surrogate for antigen anti-IgM, dem
48 Allogeneic RBCs expressing the HOD antigen (hen egg lysozyme [HEL]-ovalbumin-human transmembrane Duf
50 not limited to Smith Ag-specific B cells as hen egg lysozyme- and p-azophenylarsonate-specific B cel
52 demonstrated that globular proteins, such as hen egg lysozyme in phosphate buffered saline at room te
55 with hen's egg or cow's milk allergy; baked hen's egg or cow's milk intervention with or without a c
56 roposed that the frequent ingestion of baked hen's egg or cow's milk accelerates the resolution of he
57 determine whether the introduction of baked hen's egg or cow's milk into the diet of children with h
58 s the hypothesis that the ingestion of baked hen's egg or cow's milk results in more patients outgrow
59 genic foods: cow's milk, peanut, hard-boiled hen's egg, sesame, whitefish (cod), and wheat; the stand
61 how that the denaturing temperatures of both hen egg white lysozyme and ribonuclease A are sensitive
62 expressing either soluble or membrane-bound hen egg lysozyme (sHEL or mHEL, respectively) under cont
63 e B10.BR mice transfused with membrane-bound hen egg lysozyme antigen-transgenic RBCs also demonstrat
64 at display a cell surface Ag, membrane-bound hen egg lysozyme, strongly activate Ag-specific B cells.
65 hydrations, the low frequency conformational hen egg white lysozyme dynamics can be described by a di
67 urs after intentional hen's egg consumption, hen's egg protein levels were significantly increased in
68 nd protein and hydration dynamics of crowded hen egg white lysozyme (HEWL) labeled with a metal-carbo
69 t(R) HRT was useful as a means of diagnosing hen's egg allergy and predicting severity of induced sym
70 measured for turkey ovomucoid third domain, hen lysozyme, Bacillus circulans xylanase, and human and
71 ucted a similar experiment in which domestic hens were exposed to the mild distress of unrelated, but
72 is related to the storage stability of dried hen egg white (DEW) and its hydrolysates (HEW) in an IMF
73 nvestigated the efficacy and safety of early hen's egg introduction at age 4 to 6 months to prevent h
74 e target compounds in various types of eggs (hen, quail and duck) and honey samples (flower, forest,
75 uinone-2,6-disulfonate (AQDS(2-)) and either hen egg white lysozyme (HEWL) or bovine serum albumin (B
77 e parental recombinant strain in embryonated hen eggs, in MDCK cells, or in vivo in a mouse model.
81 nsgenic (Tg) strains were created expressing hen egg-white lysozyme (HEL) in a pancreas-specific fash
82 o-self-antigen in transgenic mice expressing hen egg lysozyme (HEL) under a retina-specific promoter.
83 the state of tolerance in Tg mice expressing hen egg lysozyme (HEL) under control of the rhodopsin pr
84 adoptively transferred into mice expressing hen egg lysozyme in their eyes, both Th1 and Th17 induce
86 generated double-transgenic mice expressing hen egg lysozyme, under the retinal interphotoreceptor r
87 vitamin D-enhanced eggs (produced by feeding hens at the maximum concentration of vitamin D3 or serum
88 h the more highly charged surfaces of folded hen egg white lysozyme (HEWL) and bovine serum albumin (
89 or peanut, 1.1 mg for cow's milk, 1.5 mg for hen's egg, 7.4 mg for cashew nut, and 0.29 mg for hazeln
90 boxylase (GAD) 206-220 or GAD 524-538 or for hen egg lysozyme 11-25 as a control in NOD, NOD-scid, an
93 CR-transgenic Th1 or Th17 cells specific for hen egg lysozyme induce ocular inflammation in recipient
95 hemical gradient of presentation of the four hen egg-white lysozyme epitopes observed in cell lines e
96 al characteristics of Cu,Zn-SOD derived from hen egg white and egg yolk were determined, and compared
99 ability to release antioxidant peptides from hen egg white and protease P was selected based on the a
100 presenting MHC class II bound peptides from hen egg white lysozyme (HEL) injected intravenously.
101 We quantitated the amounts of peptides from hen egg-white lysozyme presented by I-A(k) molecules in
102 model, we took a segment of polypeptide from hen egg lysozyme that in the native protein forms the bi
103 ive and recombinant allergenic proteins from hen's egg and cow's milk were spotted on silicon chips c
104 was detected in R. prowazekii purified from hen egg yolk sacs, and G3PDH activity was assayable in R
105 se interactions we imaged B cells (TgB) from hen egg lysozyme (HEL) transgenic mice and DC pulsed wit
106 f shell eggs were processed: one issued from hens fed with a standard diet and another receiving a di
109 promised neither the formation of functional hen egg lysozyme-specific IgM nor the secretion of free
111 4; P = .24), and 2.1% were confirmed to have hen's egg allergy versus 0.6% in the placebo group (rela
112 nheated hen's egg in 71.6% and 69.6%; heated hen's egg in 54.2% and 54.8%; milk in 23.0% and 23.3%; p
115 groups of hens were treated as follows: (i) hens were inoculated orally with Kentucky and injected i
116 pansion of Tregs did not occur in BAFF-Tg/Ig hen egg lysozyme BCR chimeras, demonstrating a requireme
117 cularly 2 weeks later with Enteritidis, (ii) hens were contact infected with Kentucky and then with E
118 th Kentucky and then with Enteritidis, (iii) hens were injected with Enteritidis only, and (iv) hens
120 thodology has been successfully validated in hen eggs, obtaining method detection limits in the range
123 common with OP-induced delayed neuropathy in hens and people but differs in the neuropathological sig
126 cteria from reproductive tissues of infected hens are packaged into the eggs and persist inside the h
131 observed in the bioaerosols of pig and layer hen confinement than the turkey confinement buildings an
135 ncriminated as the causative agent of laying hen peritonitis, Gallibacterium anatis are frequently is
138 otal of 150 18weeks old Lohmann Brown laying hens were housed in cages and fed with basal diet and fo
139 edness of 71 isolates from commercial laying hens in Iowa and 18 international reference isolates.
140 with prevalence data of Salmonella in laying hens (P < .001), broilers (P < .001), and slaughter pigs
141 istration of a SMM/TMP combination in laying hens in doses of 8 g l(-)(1) and 12 g l(-)(1) in drinkin
142 s utilizes to colonize and persist in laying hens, we used selective capture of transcribed sequences
148 nitis is the major disease problem of laying hens in commercial table egg and parent stock operations
149 sca, were supplemented to the diet of laying hens in order to increase the level of omega-3 long-chai
150 olism so we supplemented the diets of laying hens using two biofortified maize varieties with distinc
155 ggs by addition of linseed oil to the laying hens' diet has been evaluated in terms of production par
159 f response to the related homologue of ML-M, hen eggwhite lysozyme, was similar in these two groups o
160 When Clara cell secretory protein-membrane hen egg lysozyme/hemoglobin transgenic mice were crossed
161 tively diagnosed food allergy to cow's milk, hen's egg, and/or wheat were recruited at an outpatient
163 ed out for both the wild-type and the mutant hen lysozyme (TRP62GLY) to study the single mutation eff
164 ognizing antigens that are (insulin) or not (hen egg lysozyme; HEL) expressed by ss-cells have proven
167 empathy-like responses previously, observer hens showed no behavioural or physiological responses to
171 in, first identified as a minor component of hen egg white and found to be antimicrobial against Esch
172 ss a chimeric membrane protein consisting of hen egg lysozyme and a hemoglobin epitope tag under the
173 We found no evidence that consumption of hen's egg starting at 4 to 6 months of age prevents hen'
175 erport(R) HRT is useful for the diagnosis of hen's egg allergy, and may also aid in predicting the se
180 to citrullinated variants of two epitopes of hen egg-white lysozyme, a major and a minor one, bound t
181 d and real art samples for the evaluation of hen egg presence in the extract, i.e. albumen, yolk, or
182 nic (Tg) mice that express a soluble form of hen egg lysozyme (HEL) and in which B cell tolerance to
183 e-regulated expression of a secreted form of hen egg lysozyme, tagged with a murine hemoglobin (Hb) e
185 simulation, for the surprising misfolding of hen egg-white lysozyme caused by a single mutation (W62G
186 emonstrated the electrochemical nitration of hen egg white lysozyme to be at Tyr23 initially, followe
187 o examine MHC class I and II presentation of hen egg-white lysozyme (HEL) in different forms, soluble
194 obes and water on eight static structures of hen egg white lysozyme (HEWL) in various conformational
196 probe covalently attached to the surface of hen egg white lysozyme dissolved in D(2)O/glycerol solut
197 lectrochemical adsorption and voltammetry of hen-egg-white-lysozyme (HEWL) was studied at an array of
198 mpact of additional vitamin D in the diet of hens on the resulting egg vitamin D content, the effect
204 (HH26) are specific for the same epitope on hen egg white lysozyme (HEL), and share >90% sequence ho
206 ffect of the altered level of linseed oil on hens laying performance, fatty acid content and composit
207 by exposing female C57BL/6 wild-type mice or hen egg lysozyme B-cell receptor transgenic mice to desi
208 vine serum albumin, hen egg white ovalbumin, hen egg white ovomucoid, and binary mixtures of these th
214 chemically dominant peptide from the protein hen egg white lysozyme (HEL) generates different conform
215 We show here that processing of the protein hen egg-white lysozyme (HEL) resulted in citrullination
216 slationally modified peptides of the protein hen egg-white lysozyme (HEL), consisting of nitration of
217 on dynamics than a mesophilic model protein, hen egg white lysozyme (HEWL), at all measured temperatu
219 terminus of beta-catenin, the model proteins hen egg lysozyme and streptavidin, and immunoglobulins f
221 shifts of Trp C(gamma) in several proteins, hen egg white lysozyme, horse myoglobin, horse heart cyt
222 f pH and ionic strengths for three proteins: hen egg white lysozyme (HEWL), chymotrypsinogen, and T4
223 in recipient mice expressing eye-restricted hen egg lysozyme, we found important differences in the
224 mon waveguide resonance (PWR) studies showed hen egg phosphatidyl choline (PC) bilayers produce amphi
225 were crossed with mice that express soluble hen egg lysozyme and whose B cells bear receptors specif
226 mice that are double-transgenic for soluble hen egg lysozyme (HEL) and an Ig that recognizes HEL wit
227 duction in response to low levels of soluble hen egg lysozyme (HEL) both in vivo and in vitro as dete
230 chemical and rheological properties of spent hen proteins recovered by acid and alkaline extraction h
231 on on lipid oxidation of refrigerated stored hen eggs enriched with very long-chain n-3 fatty acids,
233 vaccinia virus (a smallpox virus surrogate), hen egg lysozyme, cholera toxin, ricin, and staphylococc
234 I molecules possessing a beta-chain-tethered hen egg lysosome peptide lack the Ia.2 epitope and fail
235 he second-order elastic moduli of tetragonal hen egg-white lysozyme crystals were determined as a fun
236 lysozyme auto-Ag-specific B cells in Ig(Tg) hen egg lysozyme (HEL) transgenic mice inhabit the splee
237 mounts of rHEL-C3d3 were more effective than hen egg lysozyme alone in up-regulating c-FLIP levels an
239 Our previous research has demonstrated that hens show clear behavioural and physiological responses
241 al and physiological response indicates that hens show no basis for emotional empathy in this context
244 generally larger than those observed in the hen egg white lysozyme (HEWL) ortholog, which shares 61%
245 These results support the potential of the hen as a bioreactor for the production of commercially v
246 gence of xenopatients and the utility of the hen model to study OvCa prevention, tumorigenesis, metas
247 without a comparator; and resolution of the hen's egg or cow's milk allergy as determined by food ch
248 These promising findings indicate that the hen is close to becoming a competitive manufacturing pla
261 g-term desensitization in treated animals to hen OVA via a dexamethasone-dependent tolerogenic mechan
263 shown that fusing complement fragment C3d to hen egg lysozyme (HEL) enhanced the immunogenicity of HE
264 children undergoing open food challenges to hen's egg or cow's milk, either fresh or extensively hea
265 established delayed-type hypersensitivity to hen OVA were immunized with an OVA-derived, MHC II-restr
268 The primary outcome was sensitization to hen's egg (increased specific serum IgE levels) by age 1
270 ells, whereas a VLR:Tmu receptor specific to hen egg lysozyme (a self-antigen with respect to chicken
271 in which TCR-transgenic Th cells specific to hen egg lysozyme (HEL) are adoptively transferred to rec
272 4 cells from TCR transgenic mice specific to hen egg lysozyme (HEL) are polarized with IL-6/TGF-beta
273 that B cells, which express BCRs specific to hen egg lysozyme (HEL) display diminished responsiveness
274 in which naive transgenic cells specific to hen egg lysozyme are adoptively transferred into recipie
275 productive tract are phenomena restricted to hens, indicating that such changes are likely to be rela
276 ng range of the silicon microarray for total hen's egg-specific IgE was comparable to the range of 0.
278 other animals, we examined whether treating hens with melatonin would affect eggshell thickness and
279 , selected physicochemical properties of two hen egg white powders (with and without hydrolysis) were
280 13 and 2014, respectively, included unheated hen's egg in 71.6% and 69.6%; heated hen's egg in 54.2%
286 5.6% of the children in the verum group were hen's egg sensitized versus 2.6% in the placebo group (p
287 research is necessary to investigate whether hen's egg allergen in house and bed dust plays a role in
288 ilot study, we wanted to investigate whether hen's egg allergen is detectable in house dust collected
289 or cow's milk into the diet of children with hen's egg or cow's milk allergies respectively leads to
290 on (8.4 +/- 1.5 days; n = 18), compared with hen egg lysozyme-immunized C57BL/6 (13.3 +/- 2.2 days; n
291 Structure determination in complex with hen egg white lysozyme revealed an extended VH binding i
292 of the type I IgNAR V domain in complex with hen egg-white lysozyme (HEL) reveals a minimal antigen-b
293 ructed homodimeric receptors in complex with hen-egg white lysozyme demonstrate how nanomolar affinit
295 sma VLRB responses of lamprey immunized with hen egg lysozyme and beta-galactosidase (beta-gal), demo
297 ty VLRA isolated from lamprey immunized with hen egg white lysozyme (HEL) in unbound and antigen-boun
298 f principle, we demonstrate this method with hen egg lysozyme that shows at least two kinetic phases
300 ohort studies; children aged 0-18 years with hen's egg or cow's milk allergy; baked hen's egg or cow'
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