ライフサイエンスコーパス: heparin-Sepharose

1 peptides encompassing 2 of 5 motifs bound to heparin-Sepharose.

2 raphy on Ni-NTA-agarose, DEAE-Toyopearl, and heparin-Sepharose.

3 mutants lost their affinities for binding to heparin-Sepharose.

4 ombinant protein on spin columns packed with heparin-sepharose.

5 ntration of NaCl required to elute FXIa from heparin-Sepharose.

6 highly virulent in cattle and cannot bind to heparin-Sepharose.

7 , heating, trypsin digestion, and binding to heparin-Sepharose.

8 ts adhesion, and Ang itself binds tightly to heparin-Sepharose.

9 BP-3) into six fragments, four of which bind heparin-Sepharose.

10 PECAM-1 failed to interact specifically with heparin-Sepharose, 3H-labeled heparin, or a heparin-bovi

11 When XO was bound to a prototypical GAG, heparin-Sepharose 6B (HS6B-XO), the rate of inactivation

12 c mice, VLDL from HuCI transgenic mice bound heparin-Sepharose, a model for cell-surface glycosaminog

13 ydrophobic interaction, lectin affinity, and heparin Sepharose affinity chromatography followed by SD

14 By using heparin-Sepharose affinity to discriminate between the m

15 ded its cell culture host range and bound to heparin-Sepharose, although it did not require cell surf

16 Using heparin-Sepharose and DNA-specific columns, we partially

17 tracts using ammonium sulfate fractionation, heparin-Sepharose and Mono Q chromatography, and BTE-aff

18 fied 5000-fold from PE-treated TFG2 cells by heparin-Sepharose and RNA affinity chromatography.

19 well, decrease both the affinity of Ang for heparin-Sepharose and the capacity of Ang to support cel

20 ty by chromatography through DEAE Sepharose, Heparin Sepharose, and phosphocellulose columns.

21 ,000-fold from rat livers by DEAE-Sepharose, heparin-Sepharose, and DNA affinity chromatography.

22 beled active fractions from the Q-Sepharose, heparin-Sepharose, and WGA-agarose also indicated only t

23 by sequential chromatography on Q-Sepharose, heparin-Sepharose, and WGA-agarose.

24 Mutant K45A eluted from heparin-Sepharose at lower NaCl concentrations than wild

25 65 and VEGF121 showed increased retention on heparin-Sepharose at pH 5.5 compared with pH 7.5.

26 nhanced activities, but they all eluted from heparin-Sepharose at significantly higher ionic strength

27 Dose-response curves and heparin-Sepharose binding suggested Ad.hFX has greater a

28 re purified sequentially with DEAE-Sephacel, heparin-Sepharose, ceramic hydroxyapatite, Mono Q, pheny

29 ombin isolated from plasma of the proband by heparin-Sepharose chromatography contained amounts of be

30 Heparin-Sepharose chromatography demonstrated that HL-LP

31 The second peak of activity from the heparin-Sepharose chromatography represented a purificat

32 Further purification by heparin-Sepharose chromatography resulted in separation

33 ted laminin-1 fragments were fractionated by heparin-Sepharose chromatography.

34 , Sephadex G-75, concanavalin A-agarose, and heparin-Sepharose chromatography.

35 ly purified from four edema-fluid samples by heparin-Sepharose chromatography.

36 Chinese hamster ovary cells and purified by heparin-Sepharose chromatography.

37 agarose affinity chromatography followed by heparin-sepharose chromatography.

38 le for complexes IIa and IIb (TeRF II) using heparin-Sepharose chromatography.

39 us ST132 and purified to near homogeneity by heparin Sepharose CL-6B affinity chromatography.

40 quential DEAE-Sephacel, phenyl-Sepharose FF, heparin-Sepharose CL-6B, and Q-Sepharose FF column chrom

41 inoglycan binding, MIP-1beta-A10C binds to a heparin-Sepharose column as tightly as the wild type pro

42 Heparin-Sepharose column chromatography and analytical u

43 ells, was used to monitor purification after heparin-Sepharose column chromatography, Mono-S, and C4

44 the mutants, but not of the wild type, to a heparin-Sepharose column produced binding comparable to

45 n of extracts of ammonium-grown cells with a heparin-Sepharose column resolved them into a fraction e

46 chromatographic steps, including 2 steps of heparin-Sepharose column, is reported.

47 and FGF-2 isoforms were further purified by heparin-Sepharose column.

48 In this study we used heparin-Sepharose columns to demonstrate that PVC-211 Mu

49 reduced virulence in cattle and can bind to heparin-Sepharose columns, and vCRM8, which is highly vi

50 Using heparin-Sepharose-enriched fractions that hybridized to

51 Western blotting of heparin-Sepharose-enriched supernatant mainly detected t

52 Binding experiments using heparin-Sepharose gel demonstrated that the lipid-free 1

53 atography on Ultrogel AcA 34, DEAE-Sephacel, heparin-Sepharose, hydroxylapatite, and Ultrogel AcA 44.

54 duced has been purified using, successively, heparin-Sepharose, Mono Q, and Mono S FPLC (fast protein

55 tations did not alter either HCII binding to heparin-Sepharose or HCII inhibition of thrombin in the

56 ion markedly, but DS displaced thrombin from heparin-Sepharose, suggesting that DS and heparin share

57 ent elution profiles of APC derivatives from Heparin-Sepharose supported this conclusion.

58 TE bound better to heparin-Sepharose than 633, but this difference was not

59 Heparin-Sepharose was used to characterize heparin-GST-L

60 nalysis of GH3 nuclear proteins that bind to heparin-Sepharose, we have shown that Ets-1 and GABP, wh

61 Using heparin-Sepharose, we isolated two of the protein forms

62 the wild type recombinant fragment bound to heparin-Sepharose, where it was eluted at the same NaCl

63 from oocytes and eggs via chromatography on heparin-Sepharose, whereas we isolated chromatinized his

64 Wild-type MIP-1alpha bound to heparin-Sepharose, while three of the mutants, R18A, R46

65 The GST-VEGF-exon 7 fusion protein bound to heparin-Sepharose with a similar affinity as VEGF165 and

66 Moreover, these mutants bound to heparin-Sepharose with lower affinities.

67 ed heparin binding could be dissociated from heparin-Sepharose with much lower NaCl concentrations, i

68 omogeneity by sequential chromatography over heparin-sepharose, xanthine amino congener-agarose, and