ライフサイエンスコーパス: hexahistidine

1 ich expressed one of the ATPases tagged with hexahistidine.

2 aining the photo-tag: 4-benzoylbenzyl-glycyl-hexahistidine.

3 uitin (Ub) monomers N-terminally tagged with hexahistidine.

4 toplasmic proteins expressing the ubiquitous hexahistidine affinity handle can be specifically attach

5 Simultaneous display of a hexahistidine affinity tag, the MAL1 epitope and the gre

6 litated by the inclusion of a tag containing hexahistidine and a short biotinylation sequence.

7 m-affinity purification (TAP) approach using hexahistidine and BirA-specific biotinylation tags for i

8 ith a hexahistidine tag at the N terminus or hexahistidine and c-myc tags at the C terminus was able

9 yk91g4 tagged with a hexahistidine and DLYDDDDK peptide epitope was expressed

10 using a strategy based on two affinity tags (hexahistidine and FLAG) engineered into the N terminus o

11 ing analysis was facilitated by insertion of hexahistidine and short biotinylation sequences on the d

12 eceptors extended on the amino terminus with hexahistidine and the FLAG epitope, DYKDDDDK (H/F-A(2B))

13 te that was chemoselectively modified with a hexahistidine-appended peptide.

14 Hexahistidines are very common tags used in the affinity

15 (YARKARRQARR) at the amino-terminal end and hexahistidine at the carboxy-terminal end to generate CP

16 ) expression of a f310 fusion construct with hexahistidine at the N-terminus of the coding region all

17 Likewise, pro-sigmaK-hexahistidine chimeras demonstrated that the N-terminal

18 ce (SPR) sensor surface of recombinant human hexahistidine cyclophilin A (His-CypA) is described.

19 alkaline phosphatase was not affected by the hexahistidine epitope tag.

20 Hexahistidine epitope tags were used to create aggregati

21 Both enzymes had a hexahistidine extension at the carboxy-terminal end, use

22 gamma2 subunit containing an amino-terminal hexahistidine-FLAG affinity tag (gamma2HF).

23 The hexahistidine-FLAG sequence does not hinder the function

24 immobilized on a FLAG antibody column via a hexahistidine-FLAG-tagged gamma2 subunit, gamma2HF.

25 roduce either the native protein (DapE) or a hexahistidine fusion protein (DapE-His(6)).

26 We purified FabD as a hexahistidine fusion protein (H6-FabD) and ACP in its na

27 hanced the endocytosis of AcGFP by 150-fold (hexahistidine fusion protein) or 600-fold (decahistidine

28 onsible for this interaction, we constructed hexahistidine fusion proteins from different regions of

29 a and IHFbeta were expressed and purified as hexahistidine fusion proteins, and using electrophoretic

30 d in and purified from E. coli as N-terminal hexahistidine fusion proteins.

31 rio parahaemolyticus, tagged with C-terminal hexahistidine, has been purified to apparent homogeneity

32 Toward this end, we have constructed a hexahistidine (hexa-His)-tagged terminase holoenzyme as

33 Zn(2+) ions binds tightly but reversibly to hexahistidine (His(6)) motifs on surface-exposed protein

34 onal screen utilizes the frequently employed hexahistidine (His(6)) tag as a reporter, and measures i

35 The hexahistidine (His(6))/nickel(II)-nitrilotriacetic acid

36 JVZ-007 was initially produced with a c-myc-hexahistidine (his) tag allowing purification and detect

37 over-expressed and purified as an N-terminal hexahistidine ((His)6) tagged fusion containing one zinc

38 its tagged with either hemagglutinin (HA) or hexahistidine (His6) epitopes at the C-terminus.

39 Here we describe a double-hexahistidine (His6) tag sequence, comprising two hexahi

40 (H)H fragment was equipped with a C-terminal hexahistidine (His6) tether to facilitate the assembly o

41 y fusing the protein with one of eight tags: hexahistidine (His6), thioredoxin (Trx), small ubiquitin

42 The purified hexahistidine (His6)-tagged Pseudomonas aeruginosa RhlI

43 mB1 products monitored by anti-MtmB and anti-hexahistidine immunoblotting.

44 ts to the green fluorescent protein (GFP) or hexahistidine in sporulating B. subtilis or in Escherich

45 ell line has been produced that incorporates hexahistidine-labeled p43 into the multisynthetase compl

46 omitans DspB protein engineered to contain a hexahistidine metal-binding site at its C terminus in Es

47 Microbial growth studies indicate that the hexahistidine motif is important for preventing microbia

48 rt that CP binds Zn(II) at this site using a hexahistidine motif, completed by His103 and His105 of t

49 Either a hexahistidine or a FLAG octapeptide tag was incorporated

50 the green fluorescent protein AcGFP fused to hexahistidine or decahistidine peptides (3 microM) and N

51 Hexahistidine peptides on the engineered MBD1 function a

52 The TetA(L)-hexahistidine proteoliposomes exhibit high activities of

53 istidine (His6) tag sequence, comprising two hexahistidines separated by an 11-amino acid spacer, whi

54 nding protein and a polypeptide containing a hexahistidine sequence as the N- and C-exteins, respecti

55 Inclusion of a hexahistidine sequence permits rapid purification and se

56 binantly engineered to express an N-terminal hexahistidine sequence was expressed from a eukaryotic p

57 des an influenza hemagglutinin epitope and a hexahistidine sequence, permitting sensitive detection a

58 establish that coordination of Ni(II) at the hexahistidine site is thermodynamically preferred over Z

59 II) affinity at a biologically unprecedented hexahistidine site that forms at the S100A8/S100A9 inter

60 -length A. thaliana OEP80 with an N-terminal hexahistidine tag (alphaOEP80(1-732)).

61 h serine and the C terminus was fused with a hexahistidine tag and (ii) this protein was allowed to f

62 hypothesis, TetA(L) has been purified with a hexahistidine tag at its C terminus and reconstituted in

63 The recombinant PauR protein with a hexahistidine tag at its N terminus was purified, and sp

64 dition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of e

65 Using a soluble form of BAK with a hexahistidine tag at the C terminus and a liposomal syst

66 g in a fusion of vector sequences encoding a hexahistidine tag at the carboxyl terminus.

67 Recombinant gp65 with a hexahistidine tag at the N terminus or hexahistidine and

68 e expressed proHP14 with a carboxyl-terminal hexahistidine tag in a baculovirus/insect cell system an

69 the full-length BVDV NS5B with a C-terminal hexahistidine tag in Escherichia coli failed due to the

70 imilarly, full-length PatS with a C-terminal hexahistidine tag inhibited heterocyst formation.

71 hat recombinant, purified ICP47 containing a hexahistidine tag inhibits peptide import into microsome

72 lished using a plasmid carrying abgAB with a hexahistidine tag on the carboxyl terminus of AbgB and s

73 hip, or noncovalently bound via a C-terminal hexahistidine tag on the protein to Ni(2+)nitrilotriacet

74 e factor ectodomain via the engineering of a hexahistidine tag onto its C-terminus and its use in com

75 gs show that the catalytic properties of the hexahistidine tag should be taken into consideration whe

76 A hexahistidine tag was included at the amino terminus of

77 MsbA modified with an N-terminal hexahistidine tag was overexpressed, solubilized with a

78 combinant PRG B protein (r-PRG B) fused to a hexahistidine tag was purified and analyzed for structur

79 d 277 (following the COOH-terminal 271 and a hexahistidine tag).

80 s a recombinant protein (r-NTP) containing a hexahistidine tag, and refolded to the native structure

81 e human enzyme was cloned with an N-terminal hexahistidine tag, expressed, and purified from a bacter

82 mutants with C-terminal deletions fused to a hexahistidine tag, we determined that the processing sit

83 t human ACAT-1 protein bearing an N-terminal hexahistidine tag.

84 and possesses Mn2+-dependent activity with a hexahistidine tag.

85 nity chromatography using a carboxy-terminal hexahistidine tag.

86 38 MAPKalpha is facilitated by an N-terminal hexahistidine tag.

87 gth recombinant hTF containing an N-terminal hexahistidine tag.

88 A C-terminal hexahistidine-tag (C-His) was ligated to the alpha-isofo

89 In this study, the lipA gene and a hexahistidine tagged lipA construct (LipA-His) were over

90 Recombinant hexahistidine tagged MmDjC7 (25 kDa) was efficiently exp

91 us solution by electrostatic coupling of the hexahistidine tagged OPH (His6-OPH) and poly(ethylene gl

92 bacterial expression system for a C-terminal hexahistidine tagged version of the native enzyme, which

93 The hexahistidine-tagged AcrD protein was purified and recon

94 Hexahistidine-tagged alpha and beta apo-subunits were ex

95 garose column was dependent on expression of hexahistidine-tagged alpha(i) and resulted in purificati

96 eterotrimeric G proteins were expressed with hexahistidine-tagged alpha(i) in insect cells, a heterot

97 to the medium can block lysis by a T protein hexahistidine-tagged at its C-terminus, suggesting that

98 Recombinant hexahistidine-tagged bovine A1 adenosine receptors were

99 Ni(2+)-NTA modified lipid capable of binding hexahistidine-tagged Cdc42.

100 displacement from inside-out vesicles by the hexahistidine-tagged cytoplasmic domain of band 3, cdb3-

101 an plasminogen was expressed in E. coli as a hexahistidine-tagged fusion protein and chromatographica

102 ssed and purified from Escherichia coli as a hexahistidine-tagged fusion protein.

103 ytic site, have been expressed as individual hexahistidine-tagged fusion proteins in the baculovirus

104 ds displaying chelated nickel bound purified hexahistidine-tagged G protein heterotrimers and, subseq

105 e-tagged green fluorescent protein (GFP) and hexahistidine-tagged G proteins.

106 r could be assembled with partially purified hexahistidine-tagged gamma(2) in vitro to form a functio

107 derivatized to carry chelated nickel to bind hexahistidine-tagged green fluorescent protein (GFP) and

108 In this study, a C-terminal hexahistidine-tagged helicase domain of the HCV NS3 prot

109 Recombinant N-hexahistidine-tagged HepK (H6HepK), the cytoplasmic port

110 in, we purified and characterized C-terminal hexahistidine-tagged human CLN2p/tripeptidyl-peptidase I

111 ted the suitability of applying C-terminally hexahistidine-tagged interleukin-12 (IL-12) alpha- and b

112 ic acid (NTA)-coordinated metals avidly bind hexahistidine-tagged macromolecules, including peptides

113 A hexahistidine-tagged Methanosarcina barkeri mtmB1 gene,

114 The crystal structure of the hexahistidine-tagged mouse recombinant catalytic subunit

115 An N-terminal hexahistidine-tagged MutL was constructed which was acti

116 es were isolated from PS120 cells expressing hexahistidine-tagged NHERF-1 and NHE3 using nickel-NTA-a

117 Gel shift analysis with purified hexahistidine-tagged or native NanR detected three retar

118 Purification of the hexahistidine-tagged PrnB yields homogeneous protein tha

119 ophic yeast Pichia pastoris and purified the hexahistidine-tagged protein by immobilized metal affini

120 TviC, purified as C-terminal hexahistidine-tagged protein, shows UDP-GlcNAcA 4-epimer

121 l describes a method for capture of secreted hexahistidine-tagged proteins using expanded-bed adsorpt

122 ilotriacetic acid lipid analog that can bind hexahistidine-tagged proteins, BAK oligomers were formed

123 -five site-specific mutants were produced as hexahistidine-tagged proteins, purified, and examined fo

124 that could be used as a general protocol for hexahistidine-tagged proteins.

125 cetate ligand was immobilized for binding of hexahistidine-tagged red-fluorescing TagRFP, while an ap

126 Purified hexahistidine-tagged RpoD migrates at approximately 70 k

127 y plates were used for purifying recombinant hexahistidine-tagged Triticum aestivum (wheat) chlorophy

128 nditions to capture proteins conjugated with hexahistidine-tagged ubiquitin.

129 A hexahistidine-tagged variant of dsc19CfaE formed soluble

130 (called aasS) encodes a protein of 60 kDa, a hexahistidine-tagged version of which was readily expres

131 The hexahistidine-tagged-packaging proteins were purified to

132 Using hexahistidine tags placed at the respective ends of the

133 ides a simple approach to exposing concealed hexahistidine tags while retaining native noncovalent pr

134 both with and without sequences encoding 3'-hexahistidine tags.

135 e was fused to the amino terminus, and (3) a hexahistidine tail was added to the carboxyl terminus.

136 The FLAG epitope and hexahistidine tail were added to facilitate purification

137 inding protein (PBP) tagged by an N-terminal hexahistidine tail which was immobilized onto Co(2+)-tet