ライフサイエンスコーパス: high-content

1 ipants); moderate, 12.0% (307 participants); high content, 1.8% (45 participants).

2 icipants]; moderate, 0.8% [20 participants]; high content, 2.0% [50 participants]).

3 ging has rapidly become an emerging tool for high content analyses of cell and tissue systems.

4 High content analysis accurately monitored changes in ce

5 cluding quantification of stress granules by high content analysis and fluorescence recovery after ph

6 pogenesis at single cell resolution, we used high content analysis to quantify links between PPARgamm

7 Here, we report the development of a high content analysis-based assay to describe ER activit

8 ntrol of mitotic progression, we developed a high-content analysis (HCA) approach that combines autom

9 ods for image-derived data sets generated by high-content analysis in which complex cellular phenotyp

10 High-content analysis of primary cell cultures revealed

11 High-content analysis revealed dose-dependent accumulati

12 zed by immunofluorescence, real-time RT-PCR, high-content analysis, or Western blot analysis in the f

13 ell microarrays allowing high-throughput and high-content analysis.

14 developed a low-volume, high-throughput, and high-content analytical platform for at-line product qua

15 mple, criteria for clinical care should have high content and construct validity, timeliness, and low

16 (ssp. sinensis) from Sichuan had remarkably high contents and unique profiles of flavonol glycosides

17 Thus we suggest high-content and automated image analysis can be used fo

18 High-content and high-throughput analysis technologies,

19 phores, have considerable potential for both high-content and high-throughput testing of chemicals fo

20 With the goal of versatile, high-content, and scalable molecular phenotyping of inta

21 est this approach as a powerful extension of high content applications to study mechanisms of neurite

22 A high content approach was used to measure injury in a hy

23 Their high contents are typical particularly for inner organs

24 for Ras biological activity, we developed a high content assay for inhibitors of Ras plasma membrane

25 Computational analysis of the high-content behavioral profiles predicted 370 genetic i

26 phoproteome and challenges stemming from its high-content but low-sample-throughput nature.

27 Using a high content cell-based screen, we identified fibroblast

28 crofluidic technology to high-throughput and high-content cell-based assays.

29 r multitarget biosensing and high-throughput/high-content cell-based screening.

30 Identifying phenotypes based on high-content cellular images is challenging.

31 Here we report a high content chemical screen, revealing that H1152, a RO

32 A high-content chemical screen identified a candidate drug

33 We performed a high-content chemical screen using human pluripotent ste

34 sponse modeling of large high-throughput and high-content chemical screening data.

35 Here, we use a high-content confocal image-based short hairpin RNA scre

36 from the application of new high-throughput, high-content cytometry and sequencing technologies.

37 rt cellular biomarker characterization using high-content cytometry and single cell RNA sequencing, a

38 ity to analyze and integrate the plethora of high-content data generated by fluorescence imaging.

39 High-throughput and high-content databases are increasingly important resour

40 customized for the biological semantics and high-content density of the images.

41 We propose a new method to handle high-content discovery phosphoproteomics data on perturb

42 During a high-content drug screening effort, we identified AS6012

43 ght (CyTOF; Fluidigm) cytometry, and complex high-content experimental models including slice culture

44 The rapidly changing landscape of high-content experimental results also presents a challe

45 It entails high-content feature extraction that permits accurate au

46 A high-content flow cytometry assay further demonstrates t

47 In this work, we present a high-content framework in which time-lapse imaging, and

48 c repeats (CRISPR)-Cas9 platform for in situ high-content functional analysis of RREs.

49 ied to myriad biological questions, enabling high-content functional cytomics of primary hematologic

50 regulator of telomerase trafficking using a high-content genome-wide siRNA screen in human cells for

51 In this study, we implemented a high-content, genome-wide RNAi screen to identify pathwa

52 f cord blood (CB)-derived CD34(+) cells in a high-content growth factor screen.

53 ailored for different modalities including a high-content, high-throughput platform for screening lig

54 High-content, high-throughput screening with MCB should

55 ew these cytometric technologies, capable of high-content, high-throughput single-cell assays.

56 We developed a high content image analysis algorithm to quantify change

57 To meet this need, we developed a high content image-based short hairpin RNA screen monito

58 High-content image analysis and parameterization of the

59 High-content image analysis coupled to RNA interference

60 of immunocytochemistry, gene expression, and high-content image analysis.

61 Here we used a high-content image-based screen to identify small-molecu

62 High-content, image-based screens enable the identificat

63 and minimal-biasing methods with cell-based high content imaging and biochemical assays.

64 their cytotoxicity in HeLa cells measured by high content imaging technology.

65 Using high content imaging, we determined the effect of each t

66 live cells by either confocal microscopy or high content imaging.

67 High-content imaging (HCI) allows simultaneous measureme

68 Using a combination of high-content imaging and a mammalian membrane two-hybrid

69 nal tumor components, and is compatible with high-content imaging and high-throughput applications.

70 A genome-wide, high-content imaging approach, identified the E3 ubiquit

71 In a high-content imaging assay on L. major-infected intraper

72 Importantly, quantitative high-content imaging assays indicated that the direction

73 Here, we report the development of automated high-content imaging assays to quantitate the internaliz

74 ol, PhenoPlot, which represents quantitative high-content imaging data as easily interpretable glyphs

75 Here we describe a high-content imaging method in a zebrafish model of cryp

76 Using the multiparametric high-content imaging method, we evaluate cell viability,

77 Thus, high-content imaging of cryptococcal infection in vivo d

78 Using human keratinocytes and a robotic high-content imaging platform, we screened for chemical

79 ame, single glioblastoma cells, we integrate high-content imaging prior to the scWestern assays.

80 t cell lines using in situ hybridization and high-content imaging to screen for potentially useful th

81 me-series reverse-phase protein microarrays, high-content imaging, and flow cytometry, showed a drama

82 h-throughput screening modalities, including high-content imaging, multidimensional, signature-based

83 dress this deficiency, we have adopted a new high-content imaging-based approach capable of defining

84 endogenous vimentin upon induction of EMT by high-content imaging.

85 nce could be monitored by flow cytometry and high-content imaging.

86 Application of high-content immune profiling technologies has enormous

87 We used a high-content immunofluorescence imaging-based kinase inh

88 d Japanese encephalitis virus (JEV), using a high-content immunofluorescence screen.

89 thin cirrhotic nodules were characterized by high content in esters and sugars as well as in phosphor

90 These by-products showed a high content in glucose, citric and linoleic acids, toco

91 o its high economic value in the market, and high content in health-promoting antioxidant compounds.

92 (a) transports oxidized phospholipids with a high content in lysophosphatidylcholine.

93 pids are particularly pertinent due to their high content in melanosomal membranes.

94 Thanks to their high content in phenolic compounds, date by-products con

95 or the onset of Celiac Disease (CD) is their high content in proline residues.

96 They offer a low throughput but high content in vitro experimental platform.

97 High contents in anthocyanins, flavonol-3-O-glycosides,

98 hilic phenols (>600mgkg(-1)) were present in high contents in both VOO, for early ripening stages.

99 Thus, there is an urgent need for high-content in vitro systems that can better predict dr

100 ntileukemia agents we have designed a novel, high-content in vivo screen using genetically engineered

101 g platform (htCIP) that allows us to extract high-content information about individual cells, includi

102 ar samples or compounds per hour, and yields high-content information about the action of a drug over

103 chieve a systems-level understanding of this high-content information.

104 kinome-wide siRNA-phosphoproteomic screen, a high-content kinase inhibitor screen, and a kinase trapp

105 Here, using a high-content live-imaging small interfering RNA screen,

106 we show that OCT is capable of carrying out high-content, longitudinal assays of 3D culture treatmen

107 methods are applicable for high-resolution, high-content mapping and phenotyping of normal and patho

108 By decomposing many high content measurements into the effects of perturbati

109 s automated assay processing with label-free high-content measuring and real-time monitoring of multi

110 We present here a high-content microfluidic real-time platform as a powerf

111 High content microscopic analysis was carried out in the

112 MO chains (smt3(allR)) and subjected them to high-content microscopic screening, synthetic genetic ar

113 ion, segmentation, and feature extraction in high content microscopy experiments.

114 Quantitative high content microscopy revealed concentration-dependent

115 to allow easy navigation for non-experts in high-content microscopy and data are available for downl

116 plate reader, demonstrate its suitability to high-content microscopy in 96-well high-throughput scree

117 In this study, we used high-content microscopy on a genome-wide scale to determ

118 ypic crosstalk (PHOCOS) that is suitable for high-content microscopy or other modalities that capture

119 a Snc1-based reporter and subsequently used high-content microscopy to classify these mutants based

120 that could be incorporated into an automated high-content microscopy-based screen of kinase inhibitor

121 High-throughput/high-content microscopy-based screens are powerful tools

122 y and a wild strain using flow cytometry and high-content microscopy.

123 l interfering RNA (siRNA) screens coupled to high-content microscopy.

124 d decellularised ECM proteins with automated high-content microscopy.

125 integrating brain-wide activity imaging with high-content molecular phenotyping and volume registrati

126 apper allows for the generation of accurate, high-content, molecular specific virion models and detec

127 nduced signalling and response events with a high-content multiplex RNA-mediated interference screen

128 logy is an emerging discipline that combines high-content, multiplexed measurements with informatic a

129 We present a high content multiwell plate cell-based assay approach t

130 We propose the first such use of optical high content nanoscopy of histone epigenetic marks (epi-

131 poptosis and mitotic arrest as measured in a high content nuclear morphology assay.

132 The high content of 3,4-dihydroxyphenylalanine (Dopa) (~30 m

133 ester) nanoparticles, distinguished by their high content of a hydrophobic lactone.

134 r of purple sweet potato (PSP) is due to the high content of acylated anthocyanins.

135 The high content of alpha-helices in Tra1 enabled tracing of

136 ted using polyaluminum granulates (PAG) with high content of aluminum nanoclusters.

137 of processed products like cherry juice with high content of anthocyanins and polyphenols.

138 P40 possesses a high content of anthocyanins up to 14 mg/g dry matter.

139 diseases, has been intensified due to their high content of antimicrobial agents such as polyphenols

140 rticular displayed significant diversity and high content of antimicrobial resistance genes.

141 Red oranges have a high content of antioxidant and antiinflammatory substan

142 for 7min was the best condition to obtain a high content of antioxidant polyphenols and pleasant sen

143 ts could be used as sources of products with high content of antioxidants.

144 erent carotenoids were detected, including a high content of beta-carotene and lycopene.

145 tivars with high viscosity associated with a high content of beta-glucan suggests that they are good

146 rown in the Northeast of Brazil containing a high content of bioactive compounds and typical characte

147 ee-like taste, high antioxidant activity and high content of bioactives such as rutin and 2-O-beta-d-

148 Results showed a high content of Ca in the medicinal plant studied, follo

149 The F. vulgaris showed high content of carvacrol (29.8%) as main component.

150 emical responses at -0.75 V corresponding to high content of Cd(2+) ions loaded in TiP nanoparticles

151 The kernels showed a high content of coumarin (0.87 mgg(-1)) which is the mai

152 A hallmark feature of mast cells is their high content of cytoplasmic secretory granules filled wi

153 ssing effluents are often characterized by a high content of dissolved organic dyes, resulting in lar

154 urthermore, except for proteins containing a high content of disulfide bonds, the majority of protein

155 Hestia', 'RU024 01003' and 'RU004 04095' had high content of dry matter, soluble solids, ascorbic aci

156 Foodstuff with a high content of erucic acid is considered undesirable fo

157 nsidered as nutritionally valuable for their high content of essential fatty acids and amino acids.

158 ntional oilseeds crops because of relatively high content of fat (27.25%).

159 l data suggest that a 'healthy diet', with a high content of flavonoids indicates preventive properti

160 However, SSBs have a high content of fructose, the consumption of which acute

161 Unique characteristic is the high content of gamma-tocopherol (75.4mg/100g) and delta

162 The very high content of GlcN (30%) and low content of GlcNAc (4.

163 m HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased bat

164 em for quantifying a DNA amplicon that had a high content of guanidine and cytidine.

165 kale is a robust, cold tolerant plant with a high content of health-promoting compounds, grown at a r

166 nning process allows to produce pasta with a high content of healthy compounds and minimal effects on

167 -meat control [i.e., nondairy control with a high content of high-fat processed and unprocessed meat

168 ts that circulating MPs in CF disease have a high content of inflammatory cytokines and could increas

169 ortance and biological potential, due to its high content of K and low Na.

170 The Opole province was characterised by a high content of linoleic acid.

171 Commercial emulsifiers have a high content of lipids compared to TM.

172 as a healthy part of the human diet due its high content of long chain n-3 polyunsaturated fatty aci

173 The samples were characterised by high content of marine wax esters (55-67 mol% of lipid c

174 Buttermilk is a dairy by-product with a high content of milk fat globule membranes (MFGMs), whos

175 The high content of monosaccharides promoted the progress of

176 caught in autumn and spring proved to have a high content of n-3 fatty acids and vitamin D, and hence

177 d fatty acids was between 48 and 71% and the high content of n3 fatty acids resulted in a favourable

178 water is scarce and/or of low quality due to high content of NaCl (coastal areas or areas where under

179 vative site-directed mutants, reveals a very high content of native-like structure in the transition

180 m (goji berries) have been ascribed to their high content of nutrients and phenolics.

181 an interesting nutritional value, due to the high content of oleic acid and minor components with ant

182 proteins to water-organic mixtures with the high content of organic solvent.

183 d oils with low content of linoleic acid and high content of palmitic acid behaved exceptionally well

184 tinol provides a rationale for the unusually high content of PE and its long-chain unsaturated docosa

185 lower content of phenolic compounds and the high content of pectin.

186 conditions, the obtained extract exhibited a high content of phenolic compounds (40.782 mg gallic aci

187 A high content of phenolic compounds (8600-15000mg/kg gall

188 at the systems characterized by a relatively high content of phenolics, either natural or by addition

189 r the case of phosphophoryn (PP), due to its high content of phosphate, high affinity for Ca(2+), and

190 removed by methanol containing solvents had high content of phospholipids.

191 e prepared from marine PL of high purity and high content of PL and antioxidant and low TAG content.

192 ols (DAG) and monoacylglycerols (MAG) with a high content of polyunsaturated fatty acids (PUFA).

193 The samples had a high content of potassium and calcium, but the principal

194 Aglianico grapes are known for their high content of proanthocyanidins (PAs), which are respo

195 of a new variety of Stevia rebaudiana with a high content of rebaudioside A were pretreated with etha

196 We note challenges faced owing to its high content of repetitive elements and GC bases, as wel

197 Their large genomes are characterized by a high content of repetitive elements and large pericentro

198 of blood proteins is challenging due to its high content of salts and charged small molecules.

199 This structure contains a substantially high content of single N-N bonds that exceeds the previo

200 formulated with single-chain amphiphiles and high content of sterols have been developed.

201 rcially in the sweetener industry due to the high content of steviol glycosides in its leaves.

202 ve physicochemical properties that predict a high content of structural disorder.

203 This approach provided a high content of surface confined carboxyl groups suitabl

204 Finally, freeze-dried D. hebecarpa showed high content of target phytochemicals (319 mg/100g and 1

205 umption basically due to their traditionally high content of TFA.

206 Frutita showed a very high content of the apo-carotenoid beta-citraurin, and o

207 methoxyphenylacetic acid (MPA) pendants with high content of the cis isomer responsible for their hel

208 urement of milk cobalamin is hampered by the high content of the cobalamin-binding protein haptocorri

209 udy, and it was found that the extracts with high content of total anthocyanidins exhibited significa

210 ntent of anthocyanins, while L06P13 showed a high content of total carotenoids.

211 d the highest values which corresponded to a high content of total flavonoids, total monomeric anthoc

212 Sesbania grandiflora had a very high content of total oxalates, tannins and dietary fibe

213 All powders obtained had a high content of total polyphenols.

214 Potatoes of low as well of high content of total protein, independently on flesh co

215 g DUV microscopy and taking advantage of the high content of tryptophan residues in the sequence of P

216 ranslation, especially for polypeptides with high content of tyrosine codon such as ND4, ND5, ND6 and

217 inine (R) and an intervening sequence with a high content of tyrosines (Y).

218 rved up to 30 days even when formulas had an high content of unsaturated fatty acids and valuable Lon

219 elical hydrophobic protein with an unusually high content of valine residues.

220 Fish meat is characterized by a high content of valuable nutrients.

221 no acid analysis showed that the vicilin had high contents of acidic amino acids and a low lysine/arg

222 more attention in recent years due to their high contents of antioxidant compounds.

223 The best quality of the dried product (high contents of bioactive compounds and high intensity

224 fruits of sweet cherry are characterized by high contents of bioactive compounds.

225 mental osmotic stress by adding and removing high contents of cryoprotectants.

226 s distinct from that of European beers, with high contents of gallic acid (0.5-14.7 mg/L) and low con

227 d in pig iron production, typically contains high contents of iron and various trace metals of enviro

228 regions and are generally characterized with high contents of metal oxides.

229 High contents of phenolic and flavonoid compounds may be

230 ofiles were investigated by GC-FID revealing high contents of PUFAs and a high omega-3/omega-6 ratio.

231 The presence of high contents of quercetin derivatives in commercial sam

232 le needles and berries of J. communis showed high contents of sabinene, alpha-pinene and beta-myrcene

233 ine, phenolics, and scavenging capacity; ii) high contents of spermine, total biogenic amines and tot

234 o potential situations of the concurrence of high contents of taurine and nitrite/nitrate in the diet

235 ults in a number of industrial residues with high contents of these phytochemicals.

236 analysis coupled with LC-ESI/MS/MS detected high contents of total flavonols and anthocyanins in ber

237 reatment to make modified food starches with high contents of type 3 resistant starch (RS3).

238 High-content Opera imaging identified 53 genes whose los

239 varieties may be of particular interest when high contents or special composition of flavonoids are d

240 This high-content phenotype-based assay quantifies sprouts by

241 Here we develop high-content phenotypic screening methods for the system

242 y chip provides a promising high-throughput, high-content platform for rapid, automated, parallel qua

243 ng cannot adequately provide the support for high content platforms, which often sacrifice resolution

244 High-content polysaccharides contained in the aerogel gu

245 To this end, here we developed a high content pro-arrhythmia screening platform consistin

246 l method for constructing gene networks from high-content profiles based on a network context-depende

247 e questions at a systems level, we conducted high-content profiling of Drosophila larval locomotor be

248 High-content quantification of immunocytochemical coloca

249 High content, quantitative phenotyping of larval locomot

250 A novel high-content, quantitative, live-cell imaging assay usin

251 Additionally, using high-content RNA sequencing, we show that p53 is transcr

252 Through a high-content screen (HCS) of >9,000 small molecules, we

253 To test this, we performed a high-content screen for miRNAs that inhibit the replicat

254 tely 367,000 small molecules in an automated high-content screen using an Xi-linked GFP reporter in m

255 Using a high-content screen, we identified the cardiac glycoside

256 glutathione reductase (SmTGR) inhibitors and high content screening (HCS) aiming to discover new anti

257 High content screening (HCS) showed that Silica NP expos

258 Phenotypic high content screening against the intracellular parasit

259 This prompted us to develop a high content screening assay (HCS) and Neurite Image Qua

260 velopment of high-throughput drug profiling (high content screening or HCS) provides a large amount o

261 phagy induction was examined by a cell-based high content screening system using LysoTracker, which w

262 ave developed and optimized a 384-well-based high-content screening (HCS) assay that enables us to sc

263 based assays exist, rapid and cost-efficient high-content screening (HCS) assays within intact organi

264 induced pluripotent stem cells (hiPSCs) and high-content screening (HCS).

265 Multi-parametric high-content screening (mp-HCS) of mitochondrial toxicit

266 Here, we use a combination of high-content screening and mouse genetics to identify th

267 provides a simple and convenient method for high-content screening and pre-clinical testing of poten

268 additional targeting proteins using unbiased high-content screening approaches.

269 to develop a human embryonic stem cell-based high-content screening assay to discover small molecules

270 We therefore devised a high-content screening assay to search for inhibitors of

271 In the last decade, high-content screening based on multivariate single-cell

272 owerful and widely used method for analyzing high-content screening data.

273 genic compound identification and industrial high-content screening demands in the field of safety as

274 tor 4 alpha (HNF4alpha) positive, permitting high-content screening in a 96-well plate format.

275 We applied high-content screening methods to monitor B-cell activat

276 le motorized positioning stage for automated high-content screening microscopy and provide detailed s

277 High-content screening microscopy relies on automation i

278 hput murine embryonic stem cell (mESC)-based high-content screening of 17000 small molecules for card

279 Human embryonic stem cell-based high-content screening of 550 known signal transduction

280 Our data indicate that cell-based high-content screening of small molecule libraries for t

281 High-content screening of this library for antimitotic a

282 This robust platform now enables high-content screening of various C. elegans disease mod

283 ng EphA2 canonical signaling, we developed a high-content screening platform measuring inhibition of

284 a from several imaging modalities, including high-content screening, super-resolution and time-lapse

285 Here, we describe a novel high-content screening-based phenotypic assay that analy

286 plications for improved assay development in high-content screening.

287 ed from diversity-oriented synthesis through high-content screening.

288 y have enabled its use for light-microscopy, high-content-screening, electron-microscopy and even non

289 This study demonstrates how high-content screens can be performed in oocytes, and al

290 High-content screens permit a readout (e.g., fluorescenc

291 asurements but also enable ultraminiaturized high-content screens.

292 The advent of high-throughput/high-content single cell technologies is leading to an e

293 Learning cell identity from high-content single-cell data presently relies on human

294 ng its nontemplating roles is the absence of high content structural information for TER within the t

295 spheroid analysis makes this model ideal for high content studies of gene function in individual cell

296 We present a robot that enables high-content studies of alert adult Drosophila by combin

297 single cell lineage decisions by integrating high content super-resolution nanoscopy and imaging info

298 aditional hypothesis-driven methods but that high content system-wide efforts are required.

299 mode GCSPRI/SPCE assay can provide a rapid, high content T cell screening/characterization tool that

300 r researchers tuning tracking parameters for high-content time-lapse experiments.