ライフサイエンスコーパス: histone H1

1 ort or very long, and are depleted of linker histone (H1).

2 stigated the relationship between P-TEFb and histone H1.

3 several histone H3 marks, H2A.Z, and linker histone H1.

4 evated histone acetylation and a doubling of histone H1.

5 pecific transcription factor Pax5 and linker histone H1.

6 , present at approximately the same level as histone H1.

7 tin protein 1alpha (HP1alpha) and the linker histone H1.

8 ing selective N(6)-formylation of the linker histone H1.

9 uently packaged into chromatin that contains histone H1.

10 a similar reduction in histone H3 and linker histone H1.

11 tures, comparable to those induced by linker histone H1.

12 ition of the error-prone MHEJ include Ku and histone H1.

13 complexes, even at very low levels of input histone H1.

14 creased after compaction of this target with histone H1.

15 en Msx1 and H1b, a specific isoform of mouse histone H1.

16 o so only at near-saturation levels of input histone H1.

17 ve locus, accompanied by depletion of linker histone H1.

18 etylated H3 tail domains, and is depleted of histone H1.

19 spleen extracts displayed no activity toward histone H1.

20 mononucleosomes when free or bound by linker histone H1.

21 ing import of a constitutive nuclear protein histone H1.

22 able to interact with variants of the linker histone H1.

23 species lack the somatic form of the linker histone H1.

24 moters also contain reduced levels of linker histone H1.

25 nk between NPCs and chromatin via Nup133 and histone H1.

26 r DNA arms in complex with vertebrate linker histone H1.

27 d off-dyad binding mode of Drosophila linker histone H1.

28 n and that this preference depends on linker histone H1.

29 d are correlated with the phosphorylation of histone H1.

30 pecific prolyl isomerase, and phosphorylated histone H1.

31 d exhibit kinase activity by phosphorylating histone H1.

32 The binding of histone H1(0) to T4 bacteriophage DNA was investigated u

33 We show further that the intact histone H1-0 CTD and certain synthetic CTD fragments bin

34 series of truncation mutants of recombinant histone H1-0, we demonstrate that the H1 C-terminal doma

35 amic epigenetic states defined by the linker histone H1.0 determine which cells within a tumor can su

36 in complex with the full-length human linker histone H1.0, whose globular domain shares high sequence

37 BAF binds directly and selectively to linker histone H1.1 (among three subtypes tested) and core hist

38 s showed that C-terminal residues 108-215 of histone H1.1 and the N-terminal tail plus helix alphaN i

39 dimers bind double-stranded DNA, histone H3, histone H1.1, lamin A, and transcription regulators, plu

40 High-mobility group-I amino acids 1-90 or histone H1-2 could substitute for EBNA-1 amino acids 1-3

41 ins such as vimentin and annexin, as well as histone H1.2 and H2A, were downregulated in the high pro

42 Here we report the identification of histone H1.2 as a DME-interacting protein in a yeast two

43 We nitrated recombinant and purified Histone H1.2 chemically and subjected it to an on-blot e

44 Linker histone H1.2 has been shown to suppress p53-dependent tr

45 Histone H1.2 has only one tyrosine residue in the entire

46 These findings lead us to the idea that Histone H1.2 might be an intrinsic substrate for denitra

47 resulting substrate from RAW 264.7 cells was Histone H1.2, an isoform protein of linker histone.

48 The activity was shown to be specific for Histone H1.2, to differ from proteasome activity, and to

49 A (siRNA) knockdown of the DNA damage-linked histone, H1.2.

50 whereas the third sequence was homologous to histone H1'(22-42).

51 We identify histone H1.3 as a specific repressor for the noncoding o

52 Furthermore, we demonstrate that histone H1.3 overexpression leads to increased occupancy

53 fied in other chromatin factors (e.g. linker histone H1.4 and lysine methyltransferase G9a).

54 at target histone H3 on lysines 9 and 36 and histone H1.4 on lysine 26.

55 This includes HIST1H1E, encoding histone H1.4, which has not been associated with a devel

56 80% of the nucleosomes in chromatin contain histone H1, a protein family known to affect the structu

57 one shuttle hypothesis, we did not find that histone H1 accumulated on poly(ADP-ribose) (PAR) in vivo

58 We show that histone H1 acts as a potent repressor of ligand- and coa

59 Instead, histone H1 acts by blocking a specific step in the ER al

60 Together, our data indicate that histone H1 acts selectively to reduce the overall level

61 n histone h1 mutant showed that the maternal histone H1 allele is required for DME regulation of MEA,

62 nt of chromatosomal DNA protection by linker histone H1 also depends on the linker DNA sequence.

63 Our work supports the hypothesis that linker histone H1 and chromatin structure are important factors

64 semble the classical zigzag motif induced by histone H1 and considered important for 30-nm-fiber form

65 lished by replacing somatic histones (linker histone H1 and core histones) and the testis-specific li

66 itor staurosporine, shown to dephosphorylate histone H1 and down-regulate MMTV in cultured cells, app

67 al, as they phosphorylate substrates such as histone H1 and GST-Rb, but also their activities are hig

68 in neural progenitors have reduced levels of histone H1 and H2A variants that compromise chromatin fl

69 tin containing the abundant mammalian linker histone H1 and have a unique micrococcal nuclease digest

70 of architectural proteins such as the linker histone H1 and high-mobility-group (HMG) proteins with n

71 n that it forms specific complexes with both histone H1 and histones H3/H4.

72 Additionally, MeCP2 behaves similarly to histone H1 and HMGD1 in creating a higher-order chromati

73 Furthermore, histone H1 and MeCP2 hinder model transcription factor G

74 ic approaches to show that two such factors, histone H1 and poly(ADP-ribose) polymerase-1 (PARP-1), e

75 investigate the relationship between linker histone H1 and protein-protein interactions in the nucle

76 dexamethasone concurrently dephosphorylated histone H1 and rendered the MMTV promoter refractory to

77 and is activated by, basic proteins such as histone H1 and Tau with nm affinity, consistent with a p

78 results confirm that NASP can interact with histone H1 and that this interaction occurs with high af

79 t macroH2A, heterochromatin protein-1 (HP1), histone H1 and the high mobility group protein HMG-I/Y a

80 Histone H1 and the high-mobility group (HMG) proteins ar

81 us- and host cell-encoded factors, including histones H1 and H2A, mitochondrial and cellular single-s

82 lated with their ability to directly bind to histones H1 and H3 in vitro.

83 protein between a conserved domain found in histones H1 and H5 and the PHD zinc fingers, the CH2CH z

84 ous to the single globular domains of linker histones H1 and H5 in higher eukaryotes.

85 ly similar to the winged-helix structures of histones H1 and H5.

86 ive promoters contain TBP and high levels of histone H1, and are present to varying extents in both u

87 osphorylation of CDK2 substrates such as RB, histone H1, and DNA polymerase alpha (p70 subunit) is re

88 DNA, core histones, linker histones such as histone H1, and nonhistone chromatin-associated proteins

89 e chromatin boundary factor CTCF, the linker histone H1, and several histone modifications.

90 tone tails, recruitment and deacetylation of histone H1, and spreading of hypomethylated H3-K79 with

91 Rather, histone H1, and to a lessor extent, histones H2A and H2B

92 Multiple forms of histone H1 are found in most mammalian tissues, and dive

93 Histone deacetylase 3 (HDAC3) and linker histone H1 are involved in both chromatin compaction and

94 d that all four core histones and the linker histone (H1) are associated with the transient template.

95 Linker histones (H1) are required for the proper completion of

96 H1 linker histones (H1s) are key regulators of chromatin structure

97 We identify histone H1 as a novel P-TEFb substrate, and our results

98 We identify histone H1 as a substrate for P-TEFb involved in cellula

99 Here we identify histone H1 as an additional substrate of PAD4, localize

100 activity relative to wild-type McCPK1 using histone H1 as substrate.

101 m was assayed using myelin basic protein and histone H1 as substrates.

102 required for cytosine methylation at linker histone H1-associated heterochromatin, facilitating acce

103 Our results show that P-TEFb phosphorylates histone H1 at a specific C-terminal phosphorylation site

104 Histone H1 at endogenous physiological concentrations in

105 SirT1 interacts with and deacetylates histone H1 at lysine 26.

106 Moreover, mapping of the histone H1 binding domain suggested that it is located i

107 P (nuclear autoantigenic sperm protein) is a histone H1 binding protein expressed in all cells underg

108 g mouse NASP (mNASP), a cell cycle regulated histone H1 binding protein.

109 stone H3/H4 complexes, but did not influence histone H1 binding.

110 Histone H1 binds to linker DNA between nucleosomes, but

111 The linker histone, H1, binds both to the nucleosome, sealing the D

112 uitination of H2A did not prevent binding of histone H1 but it rather enhanced the binding of this hi

113 terminus is required for phosphorylation of histone H1 but not for other substrates.

114 ity is not affected by the binding of linker histone (H1) but is greatly influenced by the dispositio

115 IGF-I-induced phosphorylation of both RB and histone H1 by cyclin A-dependent cyclin-dependent kinase

116 lanogaster in which expression of the linker histone H1 can be down-regulated over a wide range by RN

117 Immunodepletion of histone H1 caused the assembly of aberrant elongated chr

118 We find that depletion of histone H1 changes the epigenetic signature of thousands

119 In mammals, histone H1 consists of a family of related proteins, inc

120 d that a 32-kDa polypeptide with homology to histone H1 constituted the endothelial-specific DNA bind

121 Conversion of the intermediate into histone H1-containing chromatin results in increased res

122 ults showed that uH2A was present in situ in histone H1-containing nucleosomes.

123 the interactions identified here between the histone H1 CTD and DFF40/CAD target and activate linker

124 enzymatic transfer of ADP-ribose from NAD to histone H1 (defined as trans-poly(ADP-ribosylation)) or

125 viously shown that the ability of the linker histone H1 degrees to alter chromatin structure was loca

126 We find that HMGNs counteract linker histone (H1)-dependent stabilization of higher order 'te

127 Histone H1 depletion did not significantly affect the re

128 ivity results in a long-range correlation of histone H1 distribution along the chromatin.

129 potence of SPD to replace with histone H1 in histone H1-DNA complex, which indicates the more potent

130 We find that cells lacking histone H1 do not exhibit a premature-ageing phenotype n

131 ve investigated the role of embryonic linker histone H1 during mitosis in Xenopus laevis egg extracts

132 istone that replaces the somatic-type linker histone H1 during reprogramming mediated by somatic cell

133 at Snf2h controls chromatin organization and histone H1 dynamics for the establishment of gene expres

134 Histone H1 exerts its repressive effect without inhibiti

135 Unlike core histones, the linker histone H1 family is more evolutionarily diverse, and ma

136 methods to describe the preference of linker histone H1 for nucleosomes over DNA, the ionic dependenc

137 ed sequence 1 elements, and displaces linker histone H1 from silent Afp chromatin.

138 ignals), but Ku readily displaces the linker histone, H1, from DNA.

139 Adding linker histone H1 further increased compaction of the A-tract a

140 fied previously uncharacterized members of a histone H1 gene family.

141 2 (TRF2) selectively regulates the TATA-less Histone H1 gene promoter, while TBP/TFIID targets core h

142 All of the histone H1 genes are in HIST1, which is spread over abou

143 enes, consisting of 39 genes, containing two histone H1 genes, 34 genes encoding core histone protein

144 techniques to measure the dynamic binding of histone H1-GFP to unperturbed chromatin in living cells.

145 to the single globular domain of the linker histone H1 (GH1).

146 Previously, we demonstrated that histone H1 greatly stimulates naked DNA cleavage by this

147 on (hcit), including a key lysine residue on histone H1 (H1K34hcit).

148 ociates with the embryonic isoform of linker histone H1 (H1M) in egg extracts.

149 bsence of PAR hydrolysis led to PAR-modified histones H1, H2A, and H2B.

150 SSTK phosphorylated histones H1, H2A, H2AX, and H3 but not H2B or H4 or tran

151 ells were found to covalently bind biotin to histones H1, H2A, H2B, H3, and H4.

152 the binding affinities of sNASP variants to histones H1, H3.3, H4 and H3.3/H4 complexes, sNASP uses

153 Histone H1/H5 binding produces two sites of increased di

154 We have examined the effects of HMG-D and histone H1/H5 on accessibility of nucleosomal DNA.

155 ymes in the presence or absence of HMG-D and histone H1/H5.

156 erved H1 globular domain found in the linker histones H1/H5, and (c) a coiled-coil domain near the C

157 The linker histone H1 has a fundamental role in DNA compaction.

158 Linker histone H1 has been generally viewed as a global repress

159 he rDNA; however, the function of the linker histone H1 has not been well characterized.

160 Importantly, mutants deleted for the linker histone H1 Hho1 or defective in condensin activity or af

161 major core histones (H3, H4, H2A, and H2B), histone H1 (HHO1), H2AZ (HTZ1), and centromeric H3 (CSE4

162 haromyces cerevisiae homologue of the linker histone H1, Hho1p, has two domains that are similar in s

163 We investigated the effects of histone H1, high mobility group D1 (HMGD1), and methyl C

164 which is required for a nucleosome-specific histone H1-high-mobility group B exchange event and for

165 olycations such as polylysine, polyarginine, histone H1, histones H2A-H2B, and protamine were observe

166 Other proteins, including histone H1 (HON1), XRCC1, and PARP1, participate in a 'b

167 ibosomal subunits, elongation factor 1alpha, histone H1, HSP70 and CDC48), as well as candidates whos

168 By contrast, in the presence of histone H1, hSWI/SNF slides octamers to more central pos

169 We also show that histone H1 hyperphosphorylation is dramatically increase

170 nuclear phosphoinositides and downregulating histone H1 hyperphosphorylation.

171 hat simultaneous mutation of DDM1 and linker histone H1 in Arabidopsis reproduces the strong linker-s

172 s with histones in vitro and associates with histone H1 in cells.

173 for the basic C-terminal domain of mammalian histone H1 in chromatin compaction, we speculate that HM

174 ate the impact of reduced cellular levels of histone H1 in embryonic stem cells on chromatin folding

175 showed the impotence of SPD to replace with histone H1 in histone H1-DNA complex, which indicates th

176 the organization of histone tails and linker histone H1 in nucleosomes.

177 To investigate the role of histone H1 in ovarian cancer cells, we characterize indi

178 facilitating the dissociation of the linker histone H1 in response to PRL.

179 o-modification and poly(ADP-ribosyl)ation of histone H1 in the absence of free DNA ends.

180 ysine 4 trimethylation (H3K4me3), and linker histone H1 in the chromatin-dependent control of transcr

181 Depleting the linker histone H1 in vivo leads to strong activation of these e

182 These data suggest that CDK2 phosphorylates histone H1 in vivo, resulting in a more open chromatin s

183 At the same time, the total level of linker histone H1 increased reaching the ratio of 1.3 molecules

184 cally phosphorylated the exogenous substrate histone H1, indicating that it is catalytically active.

185 stem, we demonstrate proper incorporation of histone H1 into chromatin.

186 Linker histone H1 is a core chromatin component that binds to n

187 Linker histone H1 is a protein component of chromatin and has b

188 Linker histone H1 is among the most abundant components of chro

189 Histone H1 is an abundant and essential component of chr

190 The linker histone H1 is believed to be involved in chromatin organ

191 Thus, linker histone H1 is essential in Drosophila and plays a fundam

192 Histone H1 is generally viewed as a repressor of transcr

193 Our results suggest that histone H1 is involved in DME-mediated DNA methylation a

194 The linker histone H1 is involved in maintaining higher-order chrom

195 Histone H1 is one of the most critical chromatin regulat

196 Tetrahymena thermophila macronuclear histone H1 is phosphorylated by a cdc2 kinase, and H1 ph

197 Linker histone H1 is required for mitotic chromosome architectu

198 Here we show that linker histone H1 is required for the underreplication (UR) phe

199 The cleavage stage histone H1 is the orthologue of an oocyte-specific histo

200 Histone H1 is thought to act as a general repressor of t

201 Here, we show that linker histone (H1) is involved in the global regulation of DNA

202 n import receptor for ribosomal proteins and histone H1, is involved in the process.

203 st-translational modifications of individual histone H1 isoforms directly influence the transcription

204 s suggest that dephosphorylation of specific histone H1 isoforms may contribute to the previously obs

205 Vertebrates express multiple linker histone H1 isoforms, which may function redundantly.

206 y, we find that each of the six somatic cell histone H1 isoforms, whose CTDs differ significantly in

207 iated HKMT activity toward histone H3-K27 or histone H1-K26.

208 es the initial slow and progressive phase of histone H1 kinase activation and increases the level of

209 phila Cdk2 homolog, and Roughex inhibits the histone H1 kinase activities of both cyclin A-Cdc2 and c

210 e arrest to allow for DNA synthesis and CDK2 histone H1 kinase activity.

211 cells or bacteria, dramatically upregulates histone H1 kinase activity.

212 Thr-160 within the T-loop and activated its histone H1 kinase activity.

213 B1 and cyclin F form a complex that exhibits histone H1 kinase activity.

214 Histone H1 kinase assays indicated that the delayed entr

215 the patterns and extent of methylation in a histone H1 knockout strain were virtually indistinguisha

216 Our data show that cells require normal histone H1 levels to expose their proper regulatory land

217 We suggest that HP1BP3 is a ubiquitous histone H1 like nuclear protein with distinct and non-re

218 Here we find that HP1BP3, a histone H1-like chromatin protein, specifically associat

219 s that consists of an N-terminal DNA-binding histone H1-like domain and a C-terminal DksA-like domain

220 racterization of a spermatid-specific linker histone H1-like protein (termed HILS1) in the mouse and

221 ondensed chromatin, which is maintained by a histone H1-like protein, Hc1.

222 d the stable templates, we found that linker histone H1 molecules are fewer on the transient template

223 There, the histone H1 molecules stabilize the sulfatide-fimbriae in

224 Histone H1 molecules were visualized on the microvilli o

225 SWIB-4 has a histone H1 motif next to the SWIB domain and was shown t

226 Genetic analysis of the loss-of-function histone h1 mutant showed that the maternal histone H1 al

227 l cycle regulators, including the prototypic histone H1, myocyte enhancer binding factor 2, and p53.

228 Additionally, we found that the linker histone H1 not only enhances the stability but also faci

229 also demonstrated that tyrosine nitration of Histone H1 occurs in vivo.

230 trahymena thermophila, highly phosphorylated histone H1 of growing cells becomes partially dephosphor

231 nscription system, to examine the effects of histone H1 on estrogen receptor alpha (ER alpha)-mediate

232 of chromatin, we investigated the effect of histone H1 on the nucleosome remodeling activity of huma

233 as more efficient after compaction by either histone H1 or a high salt concentration, suggesting that

234 iency of the CDK5 catalytic subunit by using histone H1 or tau as substrates, and that neurotoxicity

235 In a previous article, we demonstrated that histones (H1 or histone octamers) interact with negative

236 ty that targets different lysine residues on histones H1 or H3 in vitro.

237 ymena cells, constitutive phosphorylation of histone H1 phenocopies the loss of H1 from chromatin.

238 Remarkably, we discovered that linker histone H1 phosphorylated at S/T18 decorated the inter-c

239 Histone H1 phosphorylation affects chromatin condensatio

240 e have previously shown a connection between histone H1 phosphorylation and the transcriptional compe

241 In Tetrahymena, histone H1 phosphorylation can regulate transcription an

242 e association of p21/cyclin A/cdk2 decreased histone H1 phosphorylation in vitro, as observed in immu

243 Pin1 recruitment and localized histone H1 phosphorylation were associated with transcri

244 in vivo via chromatin remodeling complexes, histone H1 phosphorylation, and recruitment of diverse c

245 ccumulation of cells in the S/G2/M phase and histone H1 phosphorylation, E1A was relocated to the cyt

246 decondensation that strongly correlates with histone H1 phosphorylation.

247 ctivity and therefore affect replication and histone H1 phosphorylation.

248 Histone H1 plays a crucial role in stabilizing higher or

249 Because histone H1 plays a pivotal role in the structure of chro

250 Linker histone H1 plays an important role in chromatin folding

251 Thus, our results indicate that linker histone H1 plays an important role in the structure and

252 ucidate a novel mechanism whereby the linker histone H1 prevents STAT5 binding at promoter DNA, and t

253 In egg cytoplasm, histone H1 protects sperm nuclei undergoing genome-wide

254 ost-translational modification of the linker histone H1 protein (H1K34hcit), pivotal in altering loca

255 box 1 protein (HMGB1) and nucleosome linker histone H1 protein are necessary components of endotoxin

256 e H1 is the orthologue of an oocyte-specific histone H1 protein found in vertebrates.

257 b6, and immune precipitated MOB1-A exhibited histone H1 protein kinase activity.

258 Porcine histone H1 proteins isolated from piglet intestinal epit

259 n band matched sequences of human and murine histone H1 proteins.

260 he intestinal protein receptors for 987P are histone H1 proteins.

261 TPK2-HA-wt phosphorylated a peptide from Histone H1, proving that TPK2 is a functional kinase.

262 mouse and human HMGN5 proteins interact with histone H1, reduce its chromatin residence time, and can

263 ne's nuclear dynamics, we generated a mutant histone H1, referred to as M1-5, in which the five cycli

264 Tetrahymena cells, phosphorylation of linker histone H1 regulates transcription of specific genes.

265 We thus identify a novel form of histone H1 regulation through phosphorylation-dependent

266 omains that are spatially distinct from both histone H1-repressed domains and actively transcribed re

267 These results suggest that histone H1 represses recombination at the rDNA by a mech

268 Here, we show that S. cerevisiae histone H1 represses recombination at the rDNA without a

269 Furthermore, we show that mutations in histone H1 result in an increase of DNA methylation in t

270 n from B. anthracis (BaSET) methylates human histone H1, resulting in repression of NF-kappaB functio

271 Reducing the levels of histone H1 results in massive epigenetic changes and alt

272 However, histone H1 returns to chromatin and the chromatin recond

273 Histone H1 shifts the conformational landscape of the nu

274 In summary, we have discovered that linker histone H1, similar to core histones, exerts its multipl

275 with native and recombinant mouse and human histone H1 species.

276 Histone H1 stabilizes the higher-order chromatin structu

277 ivity relative to wild-type McCPK1 against a histone H1 substrate.

278 meters of CDK5/p35 and CDK5/p25 towards both histone H1, the best known substrate for both enzymes, a

279 histones, native human histones, the linker histone H1, the non-histone chromosomal protein HMGN2, a

280 The pioneer factor FoxA displaces linker histone H1, thereby keeping enhancer nucleosomes accessi

281 of HMGNs increases the chromatin binding of histone H1, thereby recruiting the histone methyltransfe

282 Determining the binding properties of histone H1 to chromatin in vivo is central to understand

283 rough interfering with the binding of linker histone H1 to the nucleosome as well as a structural bas

284 We show that MeCP2, like the repressive histone H1, traps the nucleosome in a more compact monon

285 et al. present evidence that the Drosophila histone H1 variant dBigH1 prevents premature activation

286 Histone H1 variants play key roles in the regulation of

287 clear proteins, including that of the linker histone H1 variants, the most abundant family of nucleos

288 biased proteomic approach we identify linker histone H1 variants, which are involved in the generatio

289 ifferential transcription kinetics directing histone H1 versus core histone gene expression.

290 ddition, a fraction containing a fragment of histone H1 was also found to be active.

291 Histone H1 was determinant to formation of condensed str

292 The kinetic parameters for phosphorylating histone H1 were similar for mutant and wild-type CDK2, r

293 We found that the linker histone H1, when incorporated into nucleosomes, stimulat

294 We also show that lack of histone H1, which elevates heterochromatic DNA methylati

295 We recently demonstrated that linker histone H1, which is thought to have a fundamental role

296 Histone H1, which typically displaces HMGB1 from nucleos

297 The interaction of linker histone H1 with both linear and superhelical double-stra

298 ich indicates the more potent interaction of histone H1 with DNA.

299 ccumulation of gammaH2AX and the exchange of histone H1 with HMGB1 at AIRE target gene promoters.

300 and destabilizing the association of linker histone H1 with nucleosomes.