ライフサイエンスコーパス: homocitrate

1 hesis prior to the incorporation of Mo and R-homocitrate.

2 e, 9S, 1Mo, one unidentified light atom, and homocitrate.

3 from its simple constituents, Fe, S, Mo, and homocitrate.

4 insertion of molybdenum and coordination by homocitrate.

5 binding before AcCoA and CoA released before homocitrate.

6 e centered radical possibly originating from homocitrate.

7 luster accumulating on VnfX does not contain homocitrate.

8 ster ([Fe(8)S(7)]) and FeMoco ([MoFe(7)S(9)C.homocitrate]).

9 ) and alpha-ketoglutarate (alpha-KG) to give homocitrate and CoA.

10 ion of AcCoA and alpha-ketoglutarate to give homocitrate and CoA.

11 in the hydrolysis of homocitryl-CoA to give homocitrate and CoA.

12 tyl-coenzyme A and 2-oxoglutarate to form 3R-homocitrate and coenzyme A.

13 tive vs AcCoA suggesting binding of CoA to E:homocitrate and E:alpha-ketoglutarate.

14 ain atomic contributions from Mo(4+) and the homocitrate and from the central prismane Fe sites and m

15 num cofactor (FeMo-co) composed of 7Fe-9S-Mo-homocitrate and one not-yet-identified atom, which proba

16 wn that the homologues of cis-homoaconitate, homocitrate, and (-)-threo-isohomocitrate serve as inter

17 omposed of 7 iron, 9 sulfur, 1 molybdenum, 1 homocitrate, and 1 unidentified light atom.

18 -regenerating system, dithionite, molybdate, homocitrate, and at least NifB-co (the metabolic product

19 FeMo-co is composed of 7Fe, 9S, Mo, R-homocitrate, and one unidentified light atom.

20 Here we show that Mo and homocitrate are incorporated into the Fe/S core of the F

21 2 occurs at the FeMo-cofactor, a 7Fe-9S-Mo-C-homocitrate cluster.

22 ontains an [8Fe-7S] cluster and a [7Fe-9S-Mo-homocitrate] cluster, respectively designated the P-clus

23 In the present study, we show the homocitrate-dependent transfer of (49)V label from VnfX

24 tic center of nitrogenase, the [Mo:7Fe:9S:C]:homocitrate FeMo cofactor, is a S=3/2 system with a rhom

25 a Mo/homocitrate insertase that mobilizes Mo/homocitrate for the maturation of FeMoco precursor on Ni

26 ith acetylCoA to form, respectively, the (R)-homocitrate homologues of (R)-2-hydroxy-1,2,5-pentanetri

27 enase contains molybdenum, iron, sulfur, and homocitrate in a ratio of 1:7:9:1.

28 The homocitrate incorporation reaction and the insertion of

29 we show that the Fe protein can act as a Mo/homocitrate insertase that mobilizes Mo/homocitrate for

30 level of nifV expression is induced excrete homocitrate into the growth medium.

31 gest that there is in vitro incorporation of homocitrate into the V-Fe-S cluster associated with VnfX

32 ore prior to the insertion of molybdenum and homocitrate into this core.

33 binds and reduces N2 at the [Fe7, Mo, S9, X, homocitrate] iron-molybdenum cofactor (FeMo-co).

34 The DFT calculations indicate that the homocitrate ligand of the cofactor can become monodentat

35 rate reduction is provided by a [7Fe-9S-Mo-X-homocitrate] metallocluster, where X is proposed to be a

36 We also establish that only molybdate, homocitrate, MgATP, and Fe protein are essential for FeM

37 ynthesis system, NifH, NifNE, NifB-cofactor, homocitrate, MgATP, and reductant, are present.

38 Further, we establish that Mo/homocitrate mobilization by the Fe protein likely involv

39 r atoms, an interstitial light atom, and one homocitrate molecule.

40 Structural analogues of homocitrate prevent the acetylene reduction ability of t

41 ns, together with Fe(2+), S(2-), MoO4(2-), R-homocitrate, S-adenosyl methionine, and Mg-ATP, is suffi

42 and dehydrated to cis-homoaconitate with (S)-homocitrate serving as an intermediate.

43 Homocitrate synthase (acetyl-coenzyme A: 2-ketoglutarate

44 Homocitrate synthase (acetyl-coenzyme A:2-ketoglutarate

45 Homocitrate synthase (HCS) catalyzes one of the regulate

46 Homocitrate synthase (HCS) catalyzes the first and commi

47 The enzyme homocitrate synthase (HCS) catalyzes the first step in l

48 Homocitrate synthase (HCS) catalyzes the first step of l

49 d kinetic mechanism for the histidine-tagged homocitrate synthase (HCS) from Saccharomyces cerevisiae

50 st enzyme in the alpha-aminoadipate pathway, homocitrate synthase (HCS), is the target of the feedbac

51 Lys20 was initially described as homocitrate synthase (HCS), the first enzyme in the lysi

52 The homocitrate synthase from Thermus thermophilus (TtHCS) i

53 us is unexpected given previous reports that homocitrate synthase is present in mitochondria and the

54 open reading frames are predicted to encode homocitrate synthase isozymes of 47 and 49 kDa, respecti

55 The localization of homocitrate synthase to the nucleus is unexpected given

56 For example, the lysine biosynthetic enzyme homocitrate synthase was recently shown to have unexpect

57 Homocitrate synthase was used as an example in terms of

58 t predict the regulatory kinetic behavior of homocitrate synthase were derived, and simulation of the

59 kinetic isotope effect of 1 is measured for homocitrate synthase, while a small pH-independent prima

60 ain information on the chemical mechanism of homocitrate synthase.

61 tallocluster called FeMo-cofactor [7Fe-9S-Mo-homocitrate] that exhibits an S = 3/2 EPR signal in the

62 hown to catalyze both the dehydration of (R)-homocitrate to form cis-homoaconitate, and its hydration

63 te rather than the complete isomerization of homocitrate to homoisocitrate.

64 FeMo-co from Fe(2+), S(2-), MoO4(2-), and R-homocitrate using only purified Nif proteins.

65 n-molybdenum cofactor (FeMoco) ([Mo-7Fe-9S-X-homocitrate]), whereas the other contains a presumed P c

66 MoFe protein, the FeMo-cofactor ([7Fe-9S-Mo-homocitrate-X]; FeMo-co) only after the MoFe protein has