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2 nditions), T7 RNAP slips efficiently in both homopolymeric A and U tracts, and we have found that rep
5 ity assay (SPA) beads, and after addition of homopolymeric A template, NS5B enzyme, and radiolabeled
6 ucleotide steps as well as in varying length homopolymeric A(n).T(n) repeats (A(n)-tracts, where n =
7 ppage of elongating RNA polymerase (RNAP) on homopolymeric A/T tracts in DNA represents a special typ
9 y of errors made during the transcription of homopolymeric adenine sequences, such that the A tract i
13 in protein-coding regions result in extended homopolymeric amino acid tracts, often polyglutamine or
14 ase in the Km for the dNTP substrate on both homopolymeric and heteropolymeric RNA and DNA templates.
19 ication of a specific and highly polymorphic homopolymeric C stretch (D310), located within the displ
20 five (42%) were deletions or insertions in a homopolymeric C-stretch between nucleotides 303-315 (D31
21 eoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) homopolymeric capsular polysaccharide produced by strain
23 es encoding the major sugar component of the homopolymeric capsule were up-expressed > 2.5-fold, but
27 Altered specificity was observed when the homopolymeric dA.rU tract immediately 5' of the PPT was
28 udies have characterized the role of a 16 bp homopolymeric dA:dT DNA structural element in facilitati
30 g experiments demonstrate that the MRE and a homopolymeric (dA x dT) element adjacent to the MRE are
31 d into a positioned nucleosome that exhibits homopolymeric (dA x dT)-dependent localized distortion.
32 f AMT1 autoactivation is greatly enhanced by homopolymeric (dA-dT) element (A16)-mediated nucleosomal
33 provide compelling evidence that nucleosomal homopolymeric (dA-dT) elements provide enhanced DNA acce
34 rs 2, 3, and 6 mediated association with the homopolymeric (dA.dT) COL1A1/MMP DNA consensus element.
37 nduced upon zinc finger association with the homopolymeric (dA.dT) minor groove confer context-specif
38 rdings of DNA polymerase processing multiple homopolymeric DNA templates extended over 600 s and thro
40 leic acid (NA) duplexes, from B-like form of homopolymeric DNA, to mixed sequence DNA, to DNA:RNA hyb
43 teins, however, both in its binding to short homopolymeric dT(n) oligomers (as judged by polyacrylami
44 which crystal structures are available, the homopolymeric duplex sequences poly(dA) and poly(dG), an
46 we demonstrate that four additional, novel, homopolymeric expansion proteins (polyAla, polySer, poly
48 ffer in fibrils composed of single proteins (homopolymeric fibrils) from those generated by co-polyme
49 ible correlation between the ability to form homopolymeric filaments and the accelerated rate of hydr
52 nes from ACV-selected mutants identified two homopolymeric G-C nucleotide stretches as putative hot s
53 ivo analyses, we demonstrate that a variable homopolymeric G-repeat in the leader of the TlpB chemota
54 of the activation domain and insertion of a homopolymeric glutamine stretch was used to increase tra
56 uence of deafness/Crohn's disease-associated homopolymeric glycoproteins alpha-tectorin (TECTA) and g
58 unction topics mainly related to the simpler homopolymeric IF networks composed of vimentin, and spec
59 biquitin-proteasome degradation pathway is a homopolymeric, K48-linked polyubiquitin chain: the chain
64 rase RNA template domain may be analogous to homopolymeric mutational hot spots that lead to similar
65 ation to varying degrees included synthetic, homopolymeric nucleic acids such as poly(A) and poly(dT)
67 se a model in which DNA templates containing homopolymeric nucleotide runs, when bound to DinB, are i
70 rotein is encoded by a gene that possesses a homopolymeric nucleotide tract containing eight adenine
72 regional preferences for env recombination, homopolymeric nucleotide tracts, i.e., sequences known t
73 gher than rates in the WT MA lines, although homopolymeric nucleotide-run (HP) loci composed of A:T b
74 ternating base sequence d(TG)n than with any homopolymeric oligodeoxyribonucleotide; thus, it shows a
79 erase ribozyme (RPR) function, as do derived homopolymeric peptides comprising lysine or the non-prot
83 nt aphthoviruses and cardioviruses have long homopolymeric poly(C) tracts in the 5' untranslated regi
84 nificant variability among genotypes, (ii) a homopolymeric poly(U) tract, (iii) a polypyrimidine stre
85 hier poly(U/UC) sequences, and possibly pure homopolymeric poly(U) tracts, were associated with more
87 r of guanine (G) residues contained within a homopolymeric [poly(G)]tract located upstream of the usp
89 s and increases the driving force, vis-a-vis homopolymeric polyglutamine, for forming insoluble aggre
90 overed that CAG expansion constructs express homopolymeric polyglutamine, polyalanine, and polyserine
91 eduction of interchain entanglements through homopolymeric polyQ and barriers to intermolecular assoc
92 ents a number of unique challenges: the long homopolymeric polyQ tract contains nearly identical resi
93 on of enzyme when radioactive nucleotide and homopolymeric primer/template substrates are employed.
97 ed proteins that assemble predominantly into homopolymeric (rather than heteropolymeric) fibrils, whi
98 were successfully demonstrated to sequence a homopolymeric region of a DNA template, facilitating the
99 ty resided in the core aspartate hexapeptide homopolymeric region, and MBC50 values of aspartate dipe
105 nt derivatives, for +1 and -1 base errors in homopolymeric repeat sequences of three to eight base pa
106 ersible gain and loss of single bases within homopolymeric repeats as short as 6 bases, (ii) deletion
107 tern of transcription, and the presence of G homopolymeric repeats of variable lengths upstream of th
108 -I binding, which was primarily dependent on homopolymeric ribonucleotide composition, linear structu
109 r extension; these products are subjected to homopolymeric ribonucleotide tailing at the 3' termini w
111 de a conceptual advance by defining specific homopolymeric RNA motifs within the genome of HCV and ot
112 ve in an in vitro RNA polymerase assay using homopolymeric RNA or BVDV minigenomic RNA templates.
115 data indicate that rootlets are composed of homopolymeric rootletin protofilaments bundled into vari
116 erein the rate of single-base deletions in a homopolymeric run in the LYS2 gene is 10 000-fold higher
118 s (G8 and G9) or one base deletion (G6) in a homopolymeric run of seven guanines (G string) in the ge
122 , the sequence contains motifs consisting of homopolymeric runs of amino acids found in several other
124 e error rate for one-nucleotide deletions in homopolymeric runs was similar for the polymerase with o
126 known DNA templates, the ability to quantify homopolymeric runs, and a short sequencing example of se
127 fluorescein labels, which impedes readout of homopolymeric runs, is avoided by diluting the labeled d
128 ads single nucleotide deletion mismatches in homopolymeric runs, such that the error rate is 30 singl
133 in many Gram-negative organisms that possess homopolymeric sequence repeats or motifs for site-specif
134 patients contain insertions or deletions in homopolymeric sequences in the thymidine kinase (TK) gen
136 and frequently longer than the corresponding homopolymeric sequences of the respective viral RNA temp
137 t lack TK activity due to mutations on other homopolymeric sequences, reactivation can occur via reve
138 had neither the pentanucleotide sequence nor homopolymeric sequences, yet replacement of the S-segmen
139 ce of several structural features, including homopolymeric serine and threonine residues, a putative
141 halted complexes that can readily carry out homopolymeric slippage synthesis, this study reveals tha
144 The CREB-binding protein (CBP), containing a homopolymeric stretch of 19 glutamines, was likewise fou
145 on was an insertion of an A:T base pair in a homopolymeric stretch of eight A:T base pairs, and readi
148 at the likelihood of occurrence of indels in homopolymeric stretches is strongly related to stretch l
152 eltaC RT) retains DNA polymerase activity on homopolymeric substrates and partial RNase H activity, r
156 ication wherein reiterative transcription of homopolymeric templates ensures the synthesis of long 3'
158 he length of one of these microsatellites, a homopolymeric thymidine [poly(T)] repeat, is measured in
159 In this study, we investigated the role of a homopolymeric thymine [poly(T)] tract -50 to -33 relativ
161 bstitution at the TA step or in the adjacent homopolymeric tract dramatically affected affinity and p
163 ubject to localized hypermutation in a short homopolymeric tract of adenine residues located in the N
166 C. jejuni 81-176 revealed the presence of a homopolymeric tract of G residues within a gene encoding
167 ns dca is prematurely terminated following a homopolymeric tract of G's, the length of which differs
169 d to contain an N-terminal signal peptide, a homopolymeric tract of serine residues, 36 sites of O-li
170 and deletion frameshift mutations in a short homopolymeric tract of thymine residues located in the N
171 also show that the presence of an intragenic homopolymeric tract renders the expression of a function
173 outer membrane proteins and the presence of homopolymeric tracts and dinucleotide repeats in coding
175 aled variation in the sequence and length of homopolymeric tracts found within these genes, providing
176 e apparently high rate of variation of these homopolymeric tracts may be important in the survival st
179 The genes encoding each protein contain homopolymeric tracts, suggestive of phase variation medi
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