ライフサイエンスコーパス: hybridisation

1 roup were validated with comparative genomic hybridisation.

2 ce by quantitative real-time PCR and in situ hybridisation.

3 enes and matrix RNA with fluorescent in-situ hybridisation.

4 ctures, and in assessing the consequences of hybridisation.

5 ar chemokine mRNA expression by differential hybridisation.

6 d less starting material being used for each hybridisation.

7 d plants and was chosen for study by in situ hybridisation.

8 ecies remain distinct in the face of natural hybridisation.

9 age chick embryos, using whole-mount in situ hybridisation.

10 pathways that was also confirmed by in situ hybridisation.

11 a 3H-cDNA probe against intron 1 for in situ hybridisation.

12 d groups in the spacer diminish the yield of hybridisation.

13 nd quantitated after heat shock by slot blot hybridisation.

14 ving up to 150-fold increase in the yield of hybridisation.

15 mouse chromosome 2p by fluorescence in situ hybridisation.

16 nts' HPV status with p16 staining or in-situ hybridisation.

17 tem for investigating the potential for wide hybridisation.

18 ophageal mucosa was determined using in-situ hybridisation.

19 conditions demonstrated via the solid-phase hybridisation, a 144-bp double stranded DNA (dsDNA) was

20 Array comparative genomic hybridisation (aCGH) profiling is currently the gold sta

21 cally referred for array comparative genomic hybridisation (aCGH); cases were compared with 11,277 co

22 ding close relative can result from repeated hybridisation along the invasion front and/or allele sur

23 Such wide hybridisations also challenge accepted taxonomic classif

24 Hybridisation among taxa with different ploidy levels is

25 r understanding on reproductive barriers and hybridisation among this vector's sibling incipient spec

26 polymerase chain reaction (RT-PCR) and blot hybridisation analyses reveal that exon 6.1 is utilised

27 ata, in conjunction with fluorescent in situ hybridisation analyses, suggest that Sulfolobus chromoso

28 In situ hybridisation analysis demonstrates a normal distributio

29 Tissue print hybridisation analysis of the hypocotyl revealed that th

30 DNA hybridisation analysis showed that elements related to T

31 Fluorescence in-situ hybridisation analysis was done with probes specific for

32 ample of the interplay between ploidy level, hybridisation and alien plant invasion.

33 oach presents an alternative to mRNA in situ hybridisation and allows detection of expression in an a

34 Novel targets were validated by in situ hybridisation and binding to AG in vitro and in vivo.

35 ell expression in two novel genes by in situ hybridisation and catalogue dorsal gland promoter elemen

36 cloned by screening suppression subtractive hybridisation and cDNA libraries of cotton genotypes tol

37 We used PCR followed by Southern blot hybridisation and DNA sequence analysis to detect DNAs o

38 nsin genes in Brassica napus, using northern hybridisation and dot blotting.

39 tumour genomes by array comparative genomic hybridisation and gene expression microarray analysis.

40 pocampal gyrus) and cerebellum using in-situ hybridisation and immunoautoradiography.

41 ession of HB-EGF mRNA and protein by in-situ hybridisation and immunohistochemical techniques, respec

42 genomics, mouse transgenic analysis, in situ hybridisation and immunohistochemistry to identify a hig

43 ribution of KIS in human brain using in situ hybridisation and immunohistochemistry, and quantified K

44 mbryonic intestine was determined by in-situ hybridisation and immunohistochemistry.

45 ocated in the plasma membrane, while in situ hybridisation and immunolocalisation demonstrate the pre

46 els demonstrates that for a weak inter-chain hybridisation and intra-channel electron-electron attrac

47 In situ hybridisation and Northern blot analysis have shown that

48 in tumour is controversial, and both in-situ hybridisation and PCR are commonly used; P16 immunohisto

49 was detected in the human genome by Southern hybridisation and polymerase chain reaction.

50 By hybridisation and sequencing we found that most lambda c

51 By hybridisation and sequencing we found that the patterns

52 occurred more than once in association with hybridisation and shifts in ploidy.

53 Analysis with 24-colour fluorescence in situ hybridisation and single nucleotide polymorphism (SNP) a

54 (IDMI), enhancement of the damping, via d-d hybridisation and spin-pumping across the interface, and

55 been conserved following genome duplication, hybridisation and transitions between dioecy and hermaph

56 alisation, gene expression analysis, in situ hybridisation and virus-induced gene silencing, indicate

57 n 150 experiments corresponding to over 3000 hybridisations and supports the Microarray Centre's larg

58 RT-PCR analysis, in situ hybridisation, and cell surface-labelling of neural cres

59 two different monoclonal antibodies, in-situ hybridisation, and PCR with DNA sequencing.

60 d immunohistochemistry, fluorescence in-situ hybridisation, and tissue analysis to look for multiline

61 xamined by PCR, plasmid extraction, Southern hybridisation, and transconjugation.

62 ve long been problematic because of frequent hybridisation, apomixis and presumed rapid radiation, an

63 Hybridisation appears to substantially modify the biosyn

64 a one-step cell lysis, target labelling and hybridisation approach as well as a corresponding passiv

65 until levels below the limit of non-specific hybridisation are reached 11 h after wounding.

66 rformed microarray-based comparative genomic hybridisation (array-CGH) with male and female Duroc gen

67 ith unexplained IS using comparative genomic hybridisation arrays (aCGH) (n = 44) followed by targete

68 Using plasmid Southern blot hybridisation as a secondary screen, we are able to iden

69 PSA modified electrode and then applied in a hybridisation assay to determine the concentration of th

70 Direct detection of PCR product via hybridisation assay, would facilitate the development of

71 than along it, as predicted under recurrent hybridisation at the invasion front.

72 d from Haemophilus influenzae type b using a hybridisation-based strategy.

73 ce steric interference of the support on the hybridisation behaviour of immobilised oligonucleotides.

74 olyploid speciation, which generally involve hybridisation between a native and an alien species.

75 e a widespread triploid taxon resulting from hybridisation between diploid Mimulus guttatus and tetra

76 Hybridisation between S haematobium and the cattle schis

77 se conditions for solid-phase amplification, hybridisation between short 25-mer single stranded DNA (

78 ng light-matter coupling regime rests on the hybridisation between states with different numbers of e

79 Although this is consistent with "hybridisation" between the diverging human and chimp lin

80 Iberia, would be best explained by recurrent hybridisation but this is difficult to prove because the

81 Steric hindrance in hybridisation can also be a problem if the oligonucleoti

82 When hybridisation carries a cost, natural selection is predi

83 chromosomes--comparative expressed sequence hybridisation (CESH)--to establish if any expression pat

84 gh resolution genome-wide comparative genome hybridisation (CGH) arrays were used to compare tumour a

85 nt quantities of DNA for comparative genomic hybridisation (CGH) as well as more than 90 independent

86 amplification (MLPA) or comparative genomic hybridisation (CGH) cause 4q- syndrome.

87 Microarray based comparative genomic hybridisation (CGH) experiments have been used to study

88 polymorphism (SNP) array comparative genomic hybridisation (CGH) showed mutually exclusive endoredupl

89 and we demonstrate their use for subtractive hybridisation cloning of differentially expressed cDNAs.

90 In situ hybridisation confirmed the localisation of Mel1a mRNA t

91 We confirmed that hybridisation could be further enhanced by modifying the

92 as where Bulinus snails are endemic, and how hybridisation could increase the colonisation potential

93 s linear regression models of non-normalised hybridisation data to define methylation status.

94 lisation of markers and fluorescence in situ hybridisation data.

95 l3 and Dll1 expression by double RNA in situ hybridisation demonstrates that these genes have distinc

96 Northern hybridisation did not detect cathepsin D mRNA in either

97 Differential Methylation Hybridisation (DMH) is one technique used for genome-wid

98 Hybridisation, DNA sequencing, targeted mutagenesis, and

99 bryos using whole amount and section in-situ hybridisation do not readily allow appreciation of 3-dim

100 re also frequently involved in interspecific hybridisation events as well as being produced by inters

101 The hybridisation events were monitored by electrochemical i

102 Additional microarray hybridisation experiments allowed the identification of

103 Genetic and in situ hybridisation experiments have determined that this gene

104 However, our Northern blot hybridisation experiments indicate that the main RNA spe

105 Coupled with in situ hybridisation experiments, these analyses showed that th

106 essing tissues, both by fluorescence in situ hybridisation (FISH) and by chromosome conformation capt

107 hat EGFR copy number by fluorescence in-situ hybridisation (FISH) can identify patients most likely t

108 say can be combined with fluorescent in situ hybridisation (FISH) methodology in order to investigate

109 parate validation using fluorescence in situ hybridisation (FISH) shows that the proportions of bifid

110 A fluorescence in-situ hybridisation (FISH) test was used to examine the integr

111 (standard karyotyping), fluorescent in situ hybridisation (FISH), multiplex ligation-dependent probe

112 obed with the library by fluorescent in situ hybridisation (FISH), the predominant sites of labelling

113 s of bone metastases by fluorescence in situ hybridisation (FISH).

114 13.3 [corrected] using fluorescence in situ hybridisation (FISH).

115 over 24h incubations by fluorescence in situ hybridisation (FISH).

116 ohistochemical [IHC] or fluorescence in-situ hybridisation [FISH], or both).

117 ergence was obliterated by means of advanced hybridisation, followed by a multi-generation exposure o

118 ubclonal composition by fluorescence in-situ hybridisation for 17p(TP53) or 11q(ATM) deletion.

119 tological tests such as fluorescence in-situ hybridisation for aneusomy are helpful in the diagnosis.

120 lysis was based on a suppression subtractive hybridisation forward library of B157 (tea clone infeste

121 ass (Lolium multiflorum) and genomic in situ hybridisation (GISH) was used to identify the introgress

122 We identified 72 hybrid systems for which hybridisation has been putatively associated with invasi

123 raphy of D(1) and D(2) receptors and in situ hybridisation histochemistry of D(1) and D(2) mRNA were

124 In situ hybridisation histochemistry revealed no difference in p

125 itative receptor autoradiography and in situ hybridisation histochemistry were used to study both the

126 n the rat forebrain using RT-PCR and in situ hybridisation histochemistry.

127 s were identified by XY fluorescence in-situ hybridisation in marrow-derived mesenchymal stem cells a

128 The role of interspecific hybridisation in the evolution of pest species is poorly

129 an weaken the (Ir Jeff = 1/2)-(O 2p) orbital hybridisation in the in-planar Rh-O-Ir bond networks.

130 ected concurrently with fluorescence in-situ hybridisation in the same cells of a tissue section.

131 Furthermore, using a combination of in situ hybridisation, in vivo ChIP assay and transgenic explant

132 Recent advances in fluorescent in situ hybridisation included the generation of allele-specific

133 of host-based divergence evolved faster and hybridisation-induced linkage disequilibrium decayed slo

134 sequence mismatches can have upon microarray hybridisation intensities even for long oligonucleotide

135 review of studies experimentally testing the hybridisation-invasion (H-I) hypothesis in plants, anima

136 d specificity of multiplex amplifiable probe hybridisation is demonstrated by the simultaneous assess

137 nd possible acquisition of germplasm through hybridisation is fundamental to understanding the evolut

138 Hybridisation is increasingly recognised as an important

139 Therefore, using Ventana in situ hybridisation (ISH), quantitative PCR assays and biomark

140 In situ hybridisation localised Pax-7 to mononuclear cells in th

141 Impacts of introgressive hybridisation may range from genomic erosion and species

142 Recent studies have shown that hybridisation may relax transcriptional regulation and e

143 en printed electrodes the paper demonstrates hybridisation monitoring of mecA in an "on-line" assay f

144 With the hybridisation of a complementary target sequence (BPV ta

145 n of mutated mtDNA is inhibited by selective hybridisation of a nucleic acid derivative to the single

146 avidin-coated glass slides and visualised by hybridisation of fluorescent detector oligonucleotides t

147 Southern blot hybridisation of Fugu genomic DNA confirmed the SART1 ge

148 The sites were detected by in situ hybridisation of labelled rDNA to chromosomal preparatio

149 ormalisation and is less influenced by cross-hybridisation of loci.

150 ctrode in PNA form it was possible to detect hybridisation of mecA PCR product electrochemically at c

151 The sp2 hybridisation of N(10) is small compared to other flavop

152 Hybridisation of NBR2 probes to zoo blots showed that th

153 In situ hybridisation of seven candidate biomineralisation genes

154 trate that this transition occurs due to the hybridisation of states associated with different TI fil

155 azole orange) provides an anchor, which upon hybridisation of the probe to its target becomes fluores

156 Biallelic assay by hybridisation of the RCA products with fluorescence dye-

157 ication was assessed by fluorescence in-situ hybridisation of two cores of breast tumour tissue in a

158 Combined with no negative effects of hybridisation on survival or reproductive characters in

159 Whole-mount in situ hybridisation on tailbud stage embryo reveals strong exp

160 We performed digital hybridisation on the NanoString platform to assess the r

161 the syndrome with FISH (fluorescence in-situ hybridisation) or PCR as rapid diagnostic tests.

162 ot analysis with cucumber DNA shows a simple hybridisation pattern indicating one or very few genes.

163 ired synchrony, as manifested in the in situ hybridisation patterns of the single mutants.

164 roducts that can be used directly as in situ hybridisation probes.

165 The hypothesis that interspecific hybridisation promotes invasiveness has received much re

166 candidates for the semiochemical barrier to hybridisation, providing an opportunity to characterise

167 opheles gambiae in Guinea-Bissau, where high hybridisation rates appear to be stable at least since t

168 A net inhibition of the hybridisation reaction was observed after incubation wit

169 detection of naphtol was used to detect the hybridisation reaction.

170 e used locked nucleic acid probes in in situ hybridisation reactions to study the distribution of mic

171 ptical spectroscopy, these results suggest a hybridisation-related mechanism, in which Rh doping can

172 Subsequent subtractive hybridisation removes sequences common to both, leaving

173 leptin in vitro autoradiography and in situ hybridisation, respectively.

174 gel electrophoresis and array based genomic hybridisation revealed a large-scale genomic deletion co

175 In situ mRNA hybridisation revealed accumulation of mRNA encoding eac

176 Dual in situ hybridisation revealed GPCR101 and oxytocin mRNA co-expr

177 In situ hybridisation revealed that HS 6-O-sulfotransferase is r

178 In the supraoptic nucleus, in situ hybridisation revealed that the temporal regulation of o

179 In situ hybridisation reveals overlapping expression of MAST1 an

180 We used immunohistochemistry, in-situ hybridisation, reverse transcriptase-PCR (RT-PCR), and f

181 se confirmed by central fluorescence in-situ hybridisation review suggested marginal benefit with lap

182 We have used this system in a subtractive hybridisation screen that resulted in the cloning of Xen

183 UDMAP includes data from large-scale in situ hybridisation screens (wholemount and section) and micro

184 A DNA hybridisation sensor was also assembled using a thiolate

185 Transcriptional profiling and in situ hybridisation show that JNK signalling is upregulated in

186 Fluorescent in situ hybridisation showed that in ahp2-1 male meiocytes, chro

187 Whole-mount in situ hybridisation showed that Lhx6 and Lhx7 were expressed d

188 sessed by its presence in polysomes; in situ hybridisation showed that the mRNA was localised around

189 In situ hybridisation showed that TrkB was expressed primarily i

190 e identified that, when analysed by northern hybridisation, showed different patterns of expression d

191 Analysis of BMP4 expression by in situ hybridisation shows that this growth factor is produced

192 Additional fluorescence in situ hybridisation signals indicate the presence of several h

193 sought by sequence-specific oligonucleotide hybridisation, site-directed sequencing in Caucasian and

194 describe how array-based comparative genomic hybridisation, SNP arrays, array painting and next-gener

195 The hybridisation specificity experiments further indicated

196 nt, the number and location of p53 and hTERT hybridisation spots was recorded in addition to standard

197 nd +P cultures using suppression subtractive hybridisation (SSH) followed by 454 pyrosequencing, and

198 f both moieties is dependant on target-probe hybridisation straightening the loop.

199 In situ hybridisation studies demonstrated high levels of Nav1.2

200 Early models based upon RNA hybridisation studies suggest bursting dynamics arise fr

201 d a single message of 1.5 kb, while Southern hybridisation suggests a small multigene family of relat

202 In the presence of target miRNA-21, hybridisation takes place resulting in the "activation"

203 Using in situ hybridisation TASK-1 mRNA was found to be expressed in t

204 A subtractive hybridisation technique was developed to clone cDNAs rep

205 eotide array/DNA chip technology when higher hybridisation temperatures are required, for example, to

206 ally a mecA specific probe was selected from hybridisation tests with a 3' and 5' version of a previo

207 y such genes and show by whole-mount in situ hybridisation that their expression pattern is that expe

208 per we demonstrate, using RT-PCR and in situ hybridisation, that Pax3 expression can serve as a marke

209 By in situ hybridisation, the histological distribution of high lev

210 minating seedling was carried out by in situ hybridisation; the strongest signals were observed from

211 on transport, sizeable band gaps and ease of hybridisation, they are set to become a versatile tool t

212 formation of stable double-stranded DNA, by hybridisation to a complementary sequence.

213 e used per locus and each tag is detected by hybridisation to a concatameric DNA probe labelled with

214 es, adult liver and kidney, were compared by hybridisation to a set of cDNA microarrays containing 65

215 is tailed amplicon facilitates detection via hybridisation to a surface immobilised oligonucleotide c

216 trodes of a genosensor array, and subsequent hybridisation to an enzyme labelled reporter probe.

217 tigate this possibility, we utilised in situ hybridisation to determine the effect of energetic chall

218 No hybridisation to DNA from other species was observed, su

219 We used fluid percussion injury and in situ hybridisation to evaluate the expression of NGF mRNA in

220 vered and amplified quantitatively following hybridisation to genomic DNA.

221 tokeratin 13 along with fluorescence in-situ hybridisation to identify Y-chromosome positive buccal e

222 ave used orthotopic quail grafts and in situ hybridisation to investigate the long-term fate of rhomb

223 We used fluorescence in-situ hybridisation to label X and Y chromosomes.

224 ean telomere length was calculated by in-gel hybridisation to leucocyte DNA from 56 normal individual

225 We have used the technique of in situ hybridisation to localise this receptor subunit to the l

226 omics, mouse transgenic analysis and in-situ hybridisation to predict and validate the existence of a

227 e nature of mRFP transcripts, we use in situ hybridisation to reveal the dynamic spatio-temporal patt

228 n less than 5 min at 37 degrees C via direct hybridisation to short probes immobilised on individual

229 ied the emerging technique of tissue in situ hybridisation to the analysis of the Drosophila segmenta

230 ocatalytic PtNPs were then used for covalent hybridisation to the PSA modified electrode and then app

231 Using in situ hybridisation to tissue sections, we have shown that the

232 Using in situ hybridisation to visualise sites of active her1 transcri

233 be a large-scale investigation of microarray hybridisations to murine probes with known sequence mism

234 and mating success, resulting in asymmetric hybridisation upon secondary contact.

235 Oxytocin mRNA was also measured by in situ hybridisation using a 3H- or 35S-labelled oligonucleotid

236 human eye development, we performed in situ hybridisation utilising human embryonic tissue, and obse

237 belled oligonucleotide probe against exon C: hybridisation was seen over the cytoplasm of supraoptic

238 Using mRNA in situ hybridisation, we show here that ecdysone triggers the u

239 conjunction with double-fluorescent in situ hybridisation, we show that, at the beginning of neurula

240 nd cytogenetic analysis with genomic in situ hybridisation were applied to identify alien chromatin i

241 lation on the kinetics and thermodynamics of hybridisation were measured by comparing a fully methyla

242 of the duplex, which can be used for direct hybridisation with a surface immobilised probe and an en

243 orld have revealed the presence of extensive hybridisation with both native and other introduced taxa

244 e lower level of secondary structure allowed hybridisation with complementary probes made with natura

245 Fluorescence in situ hybridisation with these cosmids was used to refine the

246 entiate there without fusion, we did in-situ hybridisation with Y and X chromosome probes labelled wi

247 Hybridisations with a set of three independent blackfly

248 Polyploidy and interspecific hybridisation (with which it is often associated) have l

249 t artificial chromosome-fluorescence in situ hybridisation (YAC-FISH) mapped the WNT7A gene to chromo

250 vable and stable linkers, giving the highest hybridisation yields for surfaces containing approximate