ライフサイエンスコーパス: hybridoma

1 ltered the duration of signaling in a T cell hybridoma.

2 ant H(V)1 channels in LK35.2 cells, a B cell hybridoma.

3 albumin to a SIINFEKL-specific CD8(+) T-cell hybridoma.

4 riable fragment (scFv) cloned from the HMFG2 hybridoma.

5 ous IgG switch mutants from an anti-Gal IgG1 hybridoma.

6 extent than the parental Ab produced by the hybridoma.

7 hologically at day 21 after injection of the hybridoma.

8 in-specific and DR4 allele-restricted T cell hybridoma.

9 se splenocytes and a CD1d-positive mouse NKT hybridoma.

10 ation of insulin to a Qa-1-restricted T cell hybridoma.

11 imed IL-2 release from Ag85B-specific T cell hybridoma.

12 cell lines, did not activate mouse iNKT cell hybridomas.

13 d in 2 different TCR alpha(-)/beta(-) T cell hybridomas.

14 or murine class II MHC-restricted CD4 T cell hybridomas.

15 II to antigen-specific MHC-restricted T cell hybridomas.

16 omplexes that could directly activate T cell hybridomas.

17 ters) of mAb-producing cell lines, including hybridomas.

18 specific B cell lines prior to generation of hybridomas.

19 hnologies of fluorescence flow cytometry and hybridomas.

20 that stimulated various dNKT, but not iNKT, hybridomas.

21 nimals without the need to make conventional hybridomas.

22 dues 62-70) to murine, DQ2-restricted T cell hybridomas.

23 B-cell response, an antibody-forming B-cell hybridoma (1H6) expressing a conserved germ line VH gene

24 One of these hybridomas, 260.8, produced a monoclonal antibody that r

25 A total of 73 anti-Dsg hybridomas (47 IgM and 26 IgG) were isolated.

26 ain complementary DNA (cDNA) of anti-GD2 IgM hybridoma 5F11 and ligated to full-length streptavidin c

27 d binding of the tetramer to a number of the hybridomas, a significant percentage remained unstainabl

28 study, we report that certain murine B cell hybridomas accumulate intracellular IgM and release larg

29 T cell hybridoma activation was measured by enzyme-linked immun

30 T cell hybridomas against an immunogenic B. pseudomallei FliC e

31 Screening of these T cell hybridomas against IKEPLUS and ribosomes enriched from I

32 Hybridoma (AGS-22M6E) and CHO (ASG-22CE) versions of enf

33 reover, after transduction into the 2 T cell hybridomas, all 4 were functional as evidenced by their

34 Strikingly, pre-FS hybridomas also exhibit evidence of antigen selection, i

35 g of the F240 mRNAs produced in the parental hybridoma and CHO cells revealed identical sequences, su

36 educing and enhancing mutants using a T cell hybridoma and found similar reducing and enhancing effec

37 es Fas death receptor expression in a T cell hybridoma and human PBL.

38 chains were isolated from the corresponding hybridoma and inserted into a replication-defective sero

39 rotein-free media that now routinely support hybridoma and mammalian cell growth, fetal bovine serum

40 we present a platform process that combines hybridoma and morphogenics technologies for the generati

41 6R (B6.56R) resulted in a loss of tolerance; hybridoma and single-cell analysis indicated an FcgammaR

42 al cells to present Ag to a T cell clone and hybridoma and the ability of highly enriched LCs to pres

43 gondii-specific, lacZ-inducible, CD4 T cell hybridoma and used it as a probe to screen a T. gondii c

44 anized control rAbs derived from anti-myelin hybridomas and anti-myelin monoclonal antibodies readily

45 Hybridomas and cDNA clones derived from the immunized mi

46 roduced a series of antigalactose (anti-Gal) hybridomas and characterized their heavy chain gene usag

47 antibody sequences from retrovirus-specific hybridomas and GC B cells from infected mice revealed Ig

48 e used functional screening of anti-human AM hybridomas and isolated a mAb, PLK-1, which inhibits AM

49 1d levels more efficiently activate NKT cell hybridomas and primary NKT cells independently of whethe

50 different purposes, including generation of hybridomas and reprogramming of somatic cells.

51 onses using T. gondii-specific CD8(+) T cell hybridomas and splenic CD8(+) immune T cells from chroni

52 lls, we generated spontaneous splenic B cell hybridomas and used a novel microscopy screen to detect

53 uces protective antibody, the IgG3-producing hybridoma, and a nonprotective IgG1-producing hybridoma

54 g target specific antibodies from bacterial, hybridoma, and B cell libraries.

55 le of activating an OVA-specific CD8+ T-cell hybridoma, and that this phenomenon is dependent on the

56 identified, characterized with cloned T-cell hybridomas, and confirmed in tetramer and ELISpot assays

57 ificity of VSG-specific T-cell lines, T-cell hybridomas, and Th cells activated during infection.

58 in the ability of the tetramer to stain the hybridomas, and there was a strong correlation between t

59 e seems to be biased, particularly among IgG hybridomas, and third, most hybridomas are mutants and e

60 Also found among 56R/kdel hybridomas are clones that have inactivated the H chain

61 ularly among IgG hybridomas, and third, most hybridomas are mutants and exhibit a bias in favor of CD

62 studies demonstrate that in a murine T cell hybridoma as well as in primary murine thymocytes, a fra

63 les of Jbeta1(DJbeta/omega) alphabeta T cell hybridomas, as compared with on the Jbeta1(omega) allele

64 C class I and MHC class II-restricted T cell hybridomas, as well as enhanced a Th2-like response by i

65 could cause some anti-HCV-antibody-producing hybridoma B cells to make less-protective antibodies.

66 In this study, a hybridoma based biosensor was developed for rapid, sensi

67 efficiently stimulated virus-specific T cell hybridomas but failed to induce alloreactive stimulation

68 pecific for I-E(d) blocked IL-2 secretion by hybridomas, but I-A(d)-specific antiserum did not.

69 When expressed in a T-cell hybridoma by retroviral vector, MS4a4B protein constitut

70 The time required to isolate hybridomas by a limiting serial-dilution, however, has r

71 Stimulation of dNKT hybridomas by microbial PG was independent of Toll-like

72 Finally, hybridoma CD4(+) T clones derived from DBA/2 recipients

73 tional screening of up to 300,000 individual hybridoma cell clones within less than a day.

74 munosorbent assay (ELISA) for rat IgG from a hybridoma cell culture.

75 The application of whole hybridoma cell directly as a sensing element in biosenso

76 xamined by using an I-A(b)-restricted T-cell hybridoma cell line (BB7) that recognizes an epitope der

77 Recombinant antibodies were produced from a hybridoma cell line secreting a monoclonal antibody spec

78 yc antibodies harvested from a cultured 9E10 hybridoma cell line without the need for further purific

79 ion technology, we generated 6 stable hetero-hybridoma cell lines from EBV-transformed B-CLL cells, a

80 Hybridoma cell lines were generated and compared for bin

81 d mononuclear cells] and epithelial and iNKT-hybridoma cell lines) have been used to determine the im

82 ese antibodies were spiked into an unrelated hybridoma cell population in a ratio of 1:10,000 we obse

83 e expression of the CD4 gene in CD4-positive hybridoma cells and double-positive thymocytes.

84 ted cells to both M. tuberculosis-specific T hybridoma cells and naive P25 M. tuberculosis T-cell rec

85 ion: in order to detect antigen specificity, hybridoma cells are incubated with a hapten-horseradish

86 ak stimulation to M. tuberculosis-specific T hybridoma cells but not naive P25 T cells.

87 he first time in present study suggests that hybridoma cells could provide a valuable tool for future

88 ogenics, could be used to improve suboptimal hybridoma cells generated by means of ex vivo immunizati

89 njected with viable MHC-incompatible 3F7.A10 hybridoma cells grown in serum-free medium mounted stron

90 Moreover, these hybridoma cells have proven suitable for genetic optimiz

91 Here we describe a system in cultured hybridoma cells in which transcription of the endogenous

92 ransduced into either TCRalphabeta-deficient hybridoma cells or Rag1-/- bone marrow progenitor cells.

93 inemia was induced by i.p. injection of 6-19 hybridoma cells producing an IgG3 cryoglobulin with rheu

94 Hundreds of labeled hybridoma cells producing monoclonal antibodies (mAbs) s

95 tuberculosis-specific CD4+ T cells or F9A6 T hybridoma cells specific for M. tuberculosis antigen (Ag

96 the folded MR1 conformer activated 2/2 MAIT hybridoma cells tested, 3) the pattern of MAIT cell acti

97 MS-1-secreting hybridoma cells were then transferred into the peritoneu

98 sor was constructed by transfecting specific hybridoma cells with aequorin reporter gene and the biol

99 15 min by co-compartmentalizing single mouse hybridoma cells, a fluorescent probe and single beads co

100 reens require the generation of immortalized hybridoma cells, as well as clonal expansion in microtit

101 detection of antibody secretions from single hybridoma cells, the enhanced time resolution revealed t

102 5HT2c serotonin receptor and derived clonal hybridoma cells, which we tested for specific antigen re

103 to present exogenous antigenic peptide to T hybridoma cells.

104 ogenic T cells to Ag paralleled those of the hybridoma cells.

105 (IS) formation by CD3-specific Ab expressing hybridoma cells.

106 exogenous lipids to CD1d-restricted Valpha14 hybridoma cells.

107 ion in CD3zeta-deficient MA5.8 murine T-cell hybridoma cells.

108 milar efficiencies for presentation to BB7 T hybridoma cells.

109 ng and MHC-II presentation, as detected by T hybridoma cells.

110 ecting antibodies secreted from single mouse hybridoma cells.

111 novel hapten-specific labeling technique of hybridoma cells.

112 microarray expression profiling of 3B4.15 T-hybridoma cells.

113 y HIV-1-infected individuals and produced by hybridoma cells.

114 First, clonally related sets of anti-DSG1 hybridomas characterize the response in individual FS pa

115 y genetic structure of hp-B6.1, the original hybridoma clone (ori-B6.1) stored frozen since 1995, a s

116 rtilage proteoglycan (aggrecan)-specific Th1 hybridoma clone 5/4E8 induced joint lesions similar to t

117 as completely abrogated by anti-alpha4beta7 (hybridoma clone DATK32) or anti-alpha4 integrins (PS/2).

118 y used to block VLA-4 function in the mouse (hybridoma clone PS/2) is not specific to VLA-4 but inhib

119 After generation of hybridoma clones and assessment of their binding and fun

120 A substantial proportion of hybridoma clones generated from L2pB1 cells reacted to d

121 To test this hypothesis, we infected human hybridoma clones producing either neutralizing or non-ne

122 asurements of the supernatants of the sorted hybridoma clones revealed that all hapten-specific hybri

123 oma clones revealed that all hapten-specific hybridoma clones secrete antibodies against the target.

124 T-selection and cloning, we established nine hybridoma clones secreting anti-apoA-I mAbs in which fou

125 luding increased yield of antibody-producing hybridoma clones, ensured monoclonality of sorted cells,

126 All of the hybridoma clones, except for a neutralizing antibody-pro

127 Purified IgEs from those hybridomas could activate a basophilic cell line to unde

128 except for a neutralizing antibody-producing hybridoma, could be infected with HCV and support virus

129 e used in vitro IEC binding assays to screen hybridomas created from B cells of unmanipulated wild-ty

130 The use of FBS in hybridoma culture media is examined here, with regards t

131 The work on analysis of rat IgG from hybridoma culture showed that the microchip-based ELISA

132 ols were developed to screen antibodies from hybridoma culture supernatants using Biacore surface pla

133 1E1 is produced in hybridoma cultures as a mixture of monomers, dimers, and

134 Using a unique panel of human hybridomas derived from memory B cells after pneumococca

135 hich is expressed by almost all IgG anti-DNA hybridomas derived from the glD42H mouse.

136 A panel of hybridoma-derived anti-EpCAM mAbs was generated and scre

137 Vaccination with hybridoma-derived autologous tumor immunoglobulin (Ig) i

138 Vaccination with patient-specific hybridoma-derived Id vaccine after chemotherapy-induced

139 kinetic profile of either transfectoma- or a hybridoma-derived ipilimumab.

140 In this work, the crystal structures of the hybridoma-derived PFA1 Fab in complex with pyro-Glu3-Abe

141 clone of anti-ganglioside antibody-secreting hybridoma developed a patchy, predominantly axonal neuro

142 Importantly, nearly 30% of the hybridomas did not stain with tetramer, and these cells

143 -/- mice with and without subcutaneous 260.8 hybridomas disclosed that this IgA did not affect B. the

144 e performed with use of the murine iNKT cell hybridoma DN32.D3.

145 Using collagen-specific T cell hybridomas engineered to overexpress either Syk, Zap-70,

146 In contrast, the hybridomas expressed a diverse third complementarity-det

147 is of TCR expression showed that >90% of the hybridomas expressed the same TCR beta-chain variable re

148 nse, we analyzed 22 MOG35-55-specific T cell hybridomas expressing distinct TCR.

149 Analysis of a panel of transfected T cell hybridomas expressing Ob1A12.TCR and CD4 indicated that

150 ited anti-70k T cell proliferation and bound hybridomas expressing the conserved CDR3 motifs.

151 antibody requires retrieval of an individual hybridoma from polyclonal mixtures of cells producing an

152 t repertoire of V(H) usage in IgM anti-dsDNA hybridomas from AID-deficient mice suggests that there i

153 We have previously shown that anti-DNA hybridomas from diseased New Zealand Black/New Zealand W

154 usage was also found in CII-reactive T cell hybridomas from DR4-transgenic mice.

155 ity complex (MHC) class II-restricted T cell hybridomas from IKEPLUS-immunized mice.

156 i-PA) and IQNLF (anti-LF), were developed as hybridomas from individuals immunized with licensed anth

157 The majority of hybridomas from lungs, but not from spleens, responded t

158 ain anti-dsDNA B cells that are recovered as hybridomas from the spleens of anti-dsDNA H chain transg

159 The analysis of hybridomas from these mice revealed that the only avenue

160 BALB/c-derived T-cell hybridomas generated against this region were CD3(+), CD

161 Four-way primary screening of 763 hybridomas generated from mice immunized with guanosine

162 Most hybridomas generated from the proliferating cells are po

163 ABX-CBL is a hybridoma-generated murine IgM monoclonal antibody again

164 In the subset of patients who received hybridoma-generated vaccines, we found that anti-idiotyp

165 After immunization and hybridoma generation, a collection of 20 mouse monoclona

166 es (cDbs) derived from the parental antibody hybridomas GK1.5 and 2.43, respectively, for (89)Zr-immu

167 studies indicate that animals implanted with hybridoma had leaky blood-nerve barrier compared to mice

168 The V(H) sequences in the infected hybridomas had a significantly higher mutation frequency

169 have since found that a highly passaged B6.1 hybridoma (hp-B6.1) resulted in antibody that has little

170 stimulate the sulfatide-reactive type II NKT hybridoma Hy19.3 in a CD1d-dependent manner.

171 Comparison of the hybridomas, identical except for TCR sequence, revealed

172 transfer these antibodies by intraperitoneal hybridoma implantation and by systemic administration of

173 In contrast to hybridoma implantation, passive transfer with systemical

174 s I MHC ligand were transduced into a T cell hybridoma in the absence or presence of coreceptors.

175 SF to both the HC gp-39 and human CII T cell hybridomas in an IFNgamma-dependent and MHC-restricted m

176 control rAbs were generated from anti-myelin hybridomas in which murine variable region sequences wer

177 late-bound PG/CD1d complexes stimulated dNKT hybridomas, indicating direct recognition by the dNKT ce

178 V(H) gene segment use by IgM hybridomas is diverse, but is restricted among IgG hybri

179 f Valpha3/Valpha8+ CD1d-restricted NK T cell hybridomas is unable to recognize sulfatide in the prese

180 eported that the Ab F240, when produced in a hybridoma, is nonneutralizing as assessed by standard ne

181 Individual hybridomas isolated from the spleen of a 10-month-old BX

182 Valpha14Jalpha18 T-cell receptor-expressing hybridoma line by dendritic cells that is CD1d-dependent

183 els at the single-cell level within a single hybridoma line was observed and high expressors could be

184 ein endothelial cells and their immortalized hybridoma line, EA.hy926.

185 We report the generation of six murine hybridoma lines producing two IgM and four IgG1 MAbs to

186 We were able to generate hybridoma lines secreting mAbs with high binding specifi

187 od and hepatic mononuclear cells, mouse MAIT hybridoma lines, HLA-DR4-transgenic mice, MAIThighHLA-DR

188 ponses to BCR ligation and, when captured as hybridoma mAb lines, maintain their dual (gp41/lipid) af

189 uch as conditioned cell culture supernatant, hybridoma media, and mouse ascites fluid.

190 diotype proteins were produced either by the hybridoma method or by expression of recombinant idiotyp

191 mory B cells of a healthy individual using a hybridoma method.

192 ross-present exogenous male Ag to the T cell hybridoma, MHH, specific for HYUty plus D(b).

193 In a mouse T cell hybridoma model, TNF attenuated T cell receptor (TCR) si

194 r from commercial sources or during a rabbit hybridoma monoclonal screening and selection process usi

195 urface protein A (PspA) to a PspA-specific T hybridoma more efficiently than macrophages.

196 We showed previously that in a CD8(+) T cell hybridoma, nonstimulatory endogenous peptides enhance T

197 f whether it has been generated via standard hybridoma or display methods.

198 with more labor-intensive and time-consuming hybridoma or recombinant DNA methods.

199 n recognized as agonists by Treg-derived TCR hybridomas or CD4+CD25+ thymocytes, containing both natu

200 Antibody discovery typically uses hybridoma- or display-based selection approaches, which

201 In vitro treatment of mouse hybridoma (PA2.1) cultures with antiYIL-6R decreased IgG

202 ns undergo receptor editing, we have studied hybridoma panels from 56R/kappa-deleted (kdel) mice.

203 and rapid assay was developed using a murine hybridoma Ped-2E9 cell model.

204 After investigation on hybridoma performance, the biosensor was constructed by

205 ibodies were isolated from phage display and hybridoma platforms by functional screening for opsonoph

206 These hybridomas predominantly used the TCR V(beta)14 and V(be

207 M, we have screened a panel of IgM-producing hybridomas prepared from peritoneal cells enriched in B-

208 T cell hybridomas produced from these joint-derived cells revea

209 BV-transformed B-CLL cells, and these hetero-hybridomas produced immunoglobulin.

210 The majority (92%) of CD8(+) T cell hybridomas produced large amounts of IFN-gamma only when

211 under these conditions, most rescued B cell hybridomas produced mAbs that lacked HIV-1 Envelope (Env

212 y and binding affinity to Stx2 as the parent hybridoma-produced 5C12.

213 tumor mass in a syngeneic host compared to a hybridoma producing a monoclonal antibody to the high mo

214 variable region genes isolated from a B cell hybridoma producing alphaGal-specific IgM Ab that make i

215 We also showed that a hybridoma producing monoclonal antibody C11C1 injected i

216 he cellular level, only 2 of 45 mouse T-cell hybridomas raised against either M. leprae or M. tubercu

217 monoclonal antibody line RS6, selected from hybridomas raised against sieve elements isolated from C

218 We generated a set of hybridomas reactive with filarial E/S products and scree

219 molecule I-EalphaAbeta(d) were used as APCs, hybridomas recognized peptide only when presented by the

220 ts from 3 high-affinity human anti-dsDNA IgG hybridomas (RH14, B3, and DIL-6) and 7 human IgM anti-DN

221 Using a hybridoma sampling approach, we found that SNF1 T cells

222 The conventional hybridoma screening and subcloning process is generally

223 In this study, mice implanted with hybridoma secreting 6-19 IgA anti-IgG2a rheumatoid facto

224 A subcutaneous hybridoma secreting IgE antibodies developed.

225 Generation of hybridomas secreting human mAbs has been previously repo

226 y human hybridoma technology, we isolated 37 hybridomas secreting human MAbs to dengue viruses from 1

227 From hybridomas secreting IgG Abs reactive with apoptotic cel

228 Three hybridomas secreting mAbs with anti-LukAB activity (desi

229 the epitopes recognized by Dob1, which is a hybridoma-secreting human immunoglobulin G2 antibody to

230 dNKT hybridomas showed distinct reactivities for diverse anti

231 mice present exogenous protein Ags to T cell hybridomas similarly well, but H2-O(-/-) DCs induce stro

232 cted macrophages were overlaid with a T cell hybridoma specific for an Ag85B epitope complexed with M

233 peptide epitopes recognized by mouse T-cell hybridomas specific for each protein did not cross-react

234 tigen-loaded FLS were cocultured with T cell hybridomas specific for immunodominant portions of human

235 ity complex-deficient background, and T cell hybridomas specific for the glycosylated and nonglycosyl

236 e points from 10 min to 4 h, in a 1B6 T cell hybridoma stimulated by a set of three myelin proteolipi

237 L-2 secretion from peptide 2-reactive T cell hybridomas stimulated with alanine-substituted peptides

238 gene segment use was diverse even among IgG hybridomas suggesting that the V(L) is less critical to

239 hared multiple R mutations with anti-DSG1 FS hybridomas, suggesting selection by the same or a simila

240 PG, lipids from both sources stimulated dNKT hybridomas, suggesting that presentation of microbial li

241 lly selected by a rapid cell-based screen of hybridoma supernatants to identify antibodies that bind

242 Hybridoma supernatants were screened for binding to a si

243 nii Five anti-OmpA MAbs were developed using hybridoma technology and showed strong binding to strain

244 active human monoclonal antibodies (MAbs) by hybridoma technology from the peripheral blood of health

245 We utilized hybridoma technology to produce fully human immunoglobul

246 Hybridoma technology was the breakthrough that enabled t

247 Since the inception of hybridoma technology, efforts to improve efficiency and

248 Using a high-efficiency human hybridoma technology, we isolated 37 hybridomas secretin

249 Using hybridoma technology, we isolated a panel of H10- and N8

250 tics from murine mAbs produced from standard hybridoma technology.

251 bs against ETN (MA-ETN) were generated using hybridoma technology.

252 myeloma fusion partner allowed us to recover hybridomas that captured naturally primed, antigen-speci

253 from 2C-TCR transgenic mice and from T cell hybridomas that expressed the 2C TCR or a high-affinity

254 zed by surface plasmon resonance, and T cell hybridomas that expressed these TCR, with or without CD8

255 monoclonal antibody (MAb) from a library of hybridomas that inhibited the binding of T. vaginalis or

256 e mice yielded splenic B cells for preparing hybridomas that secrete chimeric human IgE specific for

257 To study the etiology of FS, hybridomas that secrete either IgM or IgG (predominantly

258 tion assays with Valpha14i-positive NKT cell hybridomas that the Sphingomonas glycolipid alpha-galact

259 TCRs of 23 clonally distinct T cell hybridomas that were derived from DR1-transgenic mice an

260 alysis in mouse primary T cells and a T cell hybridoma to define the regulatory enhancers responsible

261 We have developed an IgE hybridoma to LABD97 antigen.

262 An IgE mouse hybridoma to trinitrophenyl was used as a control.

263 es how to use microengraving to screen mouse hybridomas to establish new cell lines producing unique

264 Finally, we engineer mouse hybridomas to secrete Fab' fragments instead of the whol

265 the intestines of mice bearing subcutaneous hybridoma tumors.

266 were well expressed on primary T cells or T hybridomas using a tricistronic (alpha, beta, green fluo

267 This hybridoma was injected subcutaneously in SCID mice with

268 Inhibition of presentation to the hybridoma was observed with an antigenic peptide that do

269 H14, B3, and DIL-6) and 7 human IgM anti-DNA hybridomas was also investigated.

270 -39 peptide, and a set of peptide-responsive hybridomas was derived.

271 n GAD (hGAD65)-specific CD4 T-cell lines and hybridomas was generated to serve as detection reagents

272 he antibodies secreted from the HCV-infected hybridomas was impaired.

273 , but not all, exogenous Ags to T cells or T hybridomas was significantly inhibited by DO.

274 g cells and a CRM(197)-specific mouse T-cell hybridoma, we found that the serotype of conjugated PnPS

275 Comparing different T cell hybridomas, we identified key residues on the MHCII alph

276 - or lenti-virus to IgM(+) mouse B cells and hybridomas, we induce class-switch recombination (CSR) o

277 Using this assay on engineered T-cell hybridomas, we observed approximately 85% accurate pairi

278 ybridoma, and a nonprotective IgG1-producing hybridoma were compared.

279 T cell hybridomas were also used for arthritis transfer into SC

280 Twenty-six stable hybridomas were derived from BALB/c mice immunized with

281 T cell hybridomas were established from DR1- and DR4-transgenic

282 Additionally, the IgG hybridomas were extensively mutated and the distribution

283 Seven hybridomas were found to cross-react with NF-M15-35.

284 the etiology of this disease, CD4(+) T cell hybridomas were generated from inflamed tissue-derived C

285 rigger their proliferation, V beta 4+ T cell hybridomas were generated from the lungs and spleens of

286 T cell hybridomas were generated to investigate cross-reactivit

287 T-cell hybridomas were only stimulated by peptides that encompa

288 Hybridomas were produced and clones selected for their r

289 Hybridomas were produced by fusing splenocytes with mous

290 A majority of T cell hybridomas were specific for myelin protein 0 (P0), whic

291 Hybridomas were stained with the DR4-gp-39 tetramer and

292 Human hybridomas were then generated and characterized.

293 ma (P815) cells, supported IL-2 secretion of hybridomas when substituted for syngeneic splenocytes as

294 omas is diverse, but is restricted among IgG hybridomas, where the majority uses one of two V(H) gene

295 e deaminase levels were greatest in the B6.1 hybridomas, which may explain the instability.

296 the source of B cells for the preparation of hybridomas, which secreted monoclonal human IgE antibodi

297 We isolated hybridomas with anti-GA specificity from MRL/lpr mouse k

298 by interrogating a panel of dNKT mouse cell hybridomas with lipid extracts from the pathogen Listeri

299 ation frequency than those in the uninfected hybridomas, with mutations concentrating in complementar

300 ase (Lck)-associated CD4 molecules in T-cell hybridomas would allow for the detection of subthreshold