ライフサイエンスコーパス: hydratase

1 quence that spans four exons of the fumarate hydratase.

2 ially larger degree of covalency for nitrile hydratase.

3 y redox behavior at the iron site in nitrile hydratase.

4 rtant to the catalytic activity of enoyl-CoA hydratase.

5 ntaining protein and mitochondrial aconitate hydratase.

6 required for the high kcat of the enoyl-CoA hydratase.

7 tified as the catalytic residue of enoyl-CoA hydratase.

8 , of the Mycobacterium tuberculosis fumarate hydratase.

9 te determination of the activity of fumarate hydratase.

10 and RpfF shows some relatedness to enoyl CoA hydratases.

11 gy, respectively, to other sequenced nitrile hydratases.

12 that exceeding nutrients suppress Enoyl-CoA hydratase-1 (ECHS1) activity by inducing its acetylation

13 Herein, we report that enoyl-CoA hydratase-1 (ECHS1), the enzyme involved in the oxidatio

14 Aconitase-2 and enoyl-CoA-hydratase-1 expression levels were decreased in L-NMMA-t

15 Depletion of aconitase-2 and enoyl-CoA-hydratase-1 resulted in the inhibition of the Krebs cycl

16 aldehyde dehydrogenase-1; D204 in enoyl CoA hydratase-1), as well as residues of unknown function (e

17 depletion of both aconitase-2 and enoyl-CoA-hydratase-1.

18 ntribute to renal function include enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (EHHADH) and a

19 es: fatty acyl-CoA oxidase (ACOX), enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (HD), and thio

20 l-CoA thiolase (THIO), peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (HD), peroxiso

21 noate hydrolase, 2-hydroxypenta-2,4-dienoate hydratase, 4-hydroxy-2-oxovalerate aldolase, and acetald

22 Fumarate hydratase, a component of the tricarboxylic acid cycle,

23 the iron(III) site of Fe-containing nitrile hydratase, a designed ligand PyPSH(4) with two carboxami

24 2 (formerly Brevibacterium sp. R312) nitrile hydratase, a novel non-heme iron enzyme, have a large nu

25 tion of the nitrile substrate to the nitrile hydratase active site low spin Fe(3+) center.

26 ng with mechanisms for the decarboxylase and hydratase activities.

27 The results provide evidence for a hydratase activity and set the stage to use the 3-halopr

28 on to the decarboxylase activity, MSAD has a hydratase activity as demonstrated by the MSAD-catalyzed

29 E111Q4-OD/VPH complexes, which retained full hydratase activity but had little decarboxylase activity

30 led to a 28-fold reduction in 2-butenoyl-CoA hydratase activity in a preparation of organelles.

31 results provide additional evidence for the hydratase activity of MSAD and further support for the h

32 and Arg-75 have also been implicated in the hydratase activity of MSAD in which 2-oxo-3-pentynoate i

33 a mechanism-based inhibitor activated by the hydratase activity of MSAD, have been determined.

34 singly, ECH exhibiting less than 2% residual hydratase activity retains essentially 100% beta-elimina

35 nd in Bacillus subtilis, exhibit a low level hydratase activity that converts trans-3-haloacrylates t

36 A weak enoyl-CoA hydratase activity was detected for both DpgB and DpgD.

37 sing" homologue of Glu144 fails to introduce hydratase activity with the substrate analogues.

38 A dehydrogenase also has intrinsic enoyl-CoA hydratase activity, a property of other members of the a

39 exhibit beta-methylcrotonyl-coenzyme A (CoA) hydratase activity, as we predicted.

40 H-CoA effects rapid and irreversible loss of hydratase activity.

41 ype complex, but is devoid of any detectable hydratase activity.

42 y with a trans-o-hydroxybenzylidene-pyruvate hydratase-aldolase gene (pbhC).

43 from pharmacological inhibition of fumarate hydratase (also known as fumarase).

44 transcripts, that this gene encodes fumarate hydratase, an enzyme of the tricarboxylic acid cycle.

45 been obtained for HD-CoA bound to enoyl-CoA hydratase, an enzyme system that has also previously bee

46 Fumarate hydratase, an essential enzyme in the tricarboxylic acid

47 ultifunctional enzyme that minimally encodes hydratase and dehydrogenase activities.

48 their sequence, OdaA and OdaI have predicted hydratase and dioxygenase reductase activities, respecti

49 another stable complex, PaaFG, an enoyl-CoA hydratase and enoyl-Coa isomerase, both belonging to the

50 These results imply that loss of fumarate hydratase and fumarate accumulation contribute to the ag

51 l-CoA (HD-CoA), bound to wild-type enoyl-CoA hydratase and G141P, a mutant in which a hydrogen bond t

52 en bound as an enolate to MCAD and enoyl-CoA hydratase and is used to rationalize the observation tha

53 e reactions, two of which (2-trans enoyl-CoA hydratase and L-3-hydroxyacyl-CoA dehydrogenase) are cor

54 the active site of long-chain 2,3-enoyl-CoA hydratase and long-chain 3-ketoacyl-CoA thiolase.

55 DNA-encoded mitochondrial genes for fumarate hydratase and succinate dehydrogenase have been linked t

56 des a linker domain between the NH2-terminal hydratase and the COOH-terminal 3-hydroxyacyl-CoA dehydr

57 Here we show that loss of fumarate hydratase and the subsequent accumulation of fumarate in

58 erties of 3-ketoacyl-CoA thiolase, enoyl-CoA hydratase, and delta 3-cis-delta 2-trans-enoyl-CoA isome

59 l carcinoma cells with mutations in fumarate hydratase, and in cells with normal mitochondria subject

60 as NADH peroxidase and NADH oxidase, nitrile hydratase, and the hORF6 and AhpC peroxiredoxins.

61 the metal ion in the iron-containing nitrile hydratases are conserved in this enzyme, suggesting that

62 Nitrile hydratases are enzymes involved in the conversion of nit

63 finity labeling strategy identified fumarate hydratase as the principal pharmacological target.

64 a significant change in the Km value of the hydratase as well as a 5.9- and 62-fold increase, respec

65 f the mechanism of action of other acetylene hydratases, as well as in the design of antiinfectives t

66 ichia coli enzyme 2-hydroxypentadienoic acid hydratase assembles to form a 20-nm-diameter particle co

67 yl group by 3-vinyl bacteriochlorophyllide a hydratase (BchF) followed by 3-hydroxyethyl bacteriochlo

68 phosphoenolpyruvate carboxykinase, aconitate hydratase, branched-chain alpha-keto acid dehydrogenase

69 A divergent fumarate hydratase C (fumC) gene lies further upstream.

70 protic residues is directly involved in the hydratase catalysis, the multienzyme complexes with eith

71 Enoyl-CoA hydratase catalyzes the hydration of trans-2-crotonyl-Co

72 the tricarboxylic acid cycle enzyme fumarate hydratase cause hereditary leiomyomatosis and renal cell

73 Significantly, binding to enoyl-CoA hydratase causes the chemical shifts of the C1 and C3 HD

74 d a plant resolve in the prokaryotic nitrile hydratase clade.

75 the active site of cobalt containing nitrile hydratase (Co NHase) was prepared.

76 of the crotonase family including enoyl-CoA hydratase (crotonase) and methylmalonyl-CoA decarboxylas

77 Members of the enoyl-CoA hydratase (crotonase) superfamily catalyze different ove

78 r genes encoded homologs of a 1,2-carotenoid hydratase (CrtC), an O-methyltransferase (CrtF), and two

79 e desaturase (crtU/CT0323), carotenoid 1',2'-hydratase (crtC/CT0301), and carotenoid cis-trans isomer

80 Many children with fumarate hydratase deficiency do not survive infancy or childhood

81 ittent porphyria; delta amino-levulinic acid hydratase deficiency porphyria; hereditary coproporphyri

82 so occur in the recessive condition fumarate hydratase deficiency, and some parents of people with th

83 cule metabolite that accumulates in fumarate hydratase-deficient cells, plays a key role in cell tran

84 Fumarate hydratase-deficient renal cancers are highly aggressive

85 ibute to the aggressive features of fumarate hydratase-deficient tumours.

86 tified a petunia gene encoding cinnamoyl-CoA hydratase-dehydrogenase (PhCHD), a bifunctional peroxiso

87 SD IV contains' a region homologous to yeast hydratase-dehydrogenase-epimerases and to sterol carrier

88 letion analyses confirmed that the enoyl-CoA hydratase/dehydrogenase Fox2p, the putative 3-hydroxypro

89 es show that kidney cancers lacking fumarate hydratase display increased sensitivity to agents that i

90 Isocyanide (formerly isonitrile) hydratase (EC 4.2.1.103) is an enzyme of the DJ-1 superf

91 deficiency of the enzyme fumarase (fumarate hydratase, EC 4.2.1.2) which result in autosomal recessi

92 he crystal structure of the enzyme enoyl-CoA hydratase (ECH) from rat liver with the bound substrate

93 opyl)glycine, against bovine liver enoyl-CoA hydratase (ECH) were characterized.

94 an be directly bioactivated by the enoyl-CoA hydratase (ECH) with the release of 1,2-dichloro-3,3,3-t

95 D 3q1t) has been reported to be an enoyl-CoA hydratase (ECH), but SALSA analysis shows a poor match b

96 rected inactivator of bovine liver enoyl-CoA hydratase (ECH).

97 mologous to mammalian enoyl-coenzyme A (CoA) hydratases, EchA6 is non-catalytic yet essential and bin

98 the gene encoding the 2-methyl-cis-aconitate hydratase enzyme is encoded outside the prpBCDE operon.

99 coding the tricarboxylic acid cycle fumarate hydratase enzyme.

100 ferentially conserved between hydrolases and hydratases established that this position is relevant to

101 of the active site of Fe-containing nitrile hydratase (Fe-NHase), a model complex of the NO-bound ac

102 has extensive homology to the human fumarate hydratase (FH) and encodes a 288-amino acid protein (Mw

103 enes succinate dehydrogenase (SDH), fumarate hydratase (FH) and isocitrate dehydrogenase (IDH), advan

104 uclear-encoded Krebs cycle enzymes, fumarate hydratase (FH) and succinate dehydrogenase (SDHB, -C and

105 icarboxylic acid (TCA) cycle enzyme fumarate hydratase (FH) are associated with a highly malignant fo

106 Succinate dehydrogenase (SDH) and fumarate hydratase (FH) are components of the tricarboxylic acid

107 t succinate dehydrogenase (SDH) and fumarate hydratase (FH) are tumour suppressors and which associat

108 Mutation of fumarate hydratase (FH) at 1q43 is known to cause the Mendelian s

109 icarboxylic acid (TCA) cycle enzyme fumarate hydratase (FH) cause a hereditary cancer syndrome known

110 uccinate dehydrogenase A (SDHA) and fumarate hydratase (FH) expression.

111 cation of germline mutations in the fumarate hydratase (FH) gene in European families supports it as

112 The tricarboxylic acid cycle enzyme fumarate hydratase (FH) has been identified as a tumor suppressor

113 the tricarboxylic acid cycle enzyme fumarate hydratase (FH) have been linked to an aggressive variant

114 intracellular fumarate, a result of fumarate hydratase (FH) inactivation, but it is not clear how NRF

115 Fumarate hydratase (FH) is an enzyme of the tricarboxylic acid cy

116 Patients with germline fumarate hydratase (FH) mutation are predisposed to develop aggre

117 ene encoding the Krebs cycle enzyme fumarate hydratase (FH) predispose to hereditary leiomyomatosis a

118 succinate dehydrogenase (SDH), and fumarate hydratase (FH) that produce oncometabolites that competi

119 utations, biallelic inactivation of fumarate hydratase (FH), and collagen, type IV, alpha 5 and colla

120 Expression of fumarate hydratase (FH), which regulates urine fumarate accumulat

121 l and mutation of the gene encoding fumarate hydratase (FH).

122 the tricarboxylic acid cycle enzyme fumarate hydratase (FH).

123 Germline mutations of the fumarate hydratase (FH, fumarase) gene are found in the recessive

124 study, we investigated the role of fumarate hydratase (Fh1), a key component of the mitochondrial tr

125 Fumarate hydratases (FHs) are essential metabolic enzymes grouped

126 Monofunctional 2-hydroxypentadienoic acid hydratase from Escherichia coli has been purified 3800-f

127 r crystallization of two proteins, enoyl-CoA hydratase from Mycobacterium tuberculosis and dihydrofol

128 r crystallization of two proteins, enoyl-CoA hydratase from Mycobacterium tuberculosis and dihydrofol

129 cond and third shells of the Co-type nitrile hydratase from Pseudomonas putida (ppNHase) that may be

130 Nitrile hydratase from Pseudomonas putida NRRL-18668 has been pu

131 data were obtained for the iron-type nitrile hydratase from Rhodococcus equi TG328-2 (ReNHase) using

132 The alphabeta dimer of active nitrile hydratase from Rhodococcus sp. R312 contains one low-spi

133 The absence of nitrile hydratase from several sequenced species indicates that

134 The fungal-specific enzyme homoaconitate hydratase from this pathway is moderately similar to the

135 requires a 4-carboxy-2-hydroxymuconate (CHM) hydratase (GalB), which has a 12% sequence identity to a

136 Germline mutations in the fumarate hydratase gene (FH) predispose to multiple cutaneous and

137 The nitrile hydratase gene in M. brevicollis was believed to have ar

138 nce presented here demonstrates that nitrile hydratase genes are present in multiple eukaryotic super

139 NGS: Here we report the detection of nitrile hydratase genes in five eukaryotic supergroups: opisthok

140 ATP-synthase delta chain and Enoyl-CoenzymeA hydratase, glutathione-s-transferase omega, alpha-1-acid

141 yme A (HD-CoA) bound to the enzyme enoyl-CoA hydratase has been determined using transferred nuclear

142 64 and E144) in the active site of enoyl-CoA hydratase has been probed by site-directed mutagenesis.

143 9 and 9.2, whereas the Glu119 --> Gln mutant hydratase has only a single pKa of 9.5.

144 they are widespread in prokaryotes, nitrile hydratases have only been reported in two eukaryotes: th

145 of the PPARalpha target genes rat enoyl-CoA hydratase (HD) and peroxisomal fatty acyl-CoA oxidase (A

146 derstand the structural basis for isocyanide hydratase (ICH) catalysis, we determined the crystal str

147 2 (formerly Brevibacterium sp. R312) nitrile hydratase in frozen solutions at pH 7 and 9 has been ana

148 The presence of nitrile hydratases in many other eukaryotic groups is unresolved

149 s recover a clade of eukaryotic-type nitrile hydratases in the Opisthokonta, Amoebozoa, SAR and CCTH;

150 family of nonheme iron enzymes, the nitrile hydratases, in which post-translational oxidation of two

151 NO-sensitive [4Fe-4S] (de)hydratases, including the Krebs cycle aconitase and the

152 These data suggest that enoyl-CoA hydratase is an important enzyme in the bioactivation of

153 Loss of fumarate hydratase is associated with suppression of miR-200 and

154 2-Hydroxypentadienoic acid hydratase is found on many bacterial catabolic pathways

155 Activity of fumarate hydratase is reduced in lymphoblastoid cells from indivi

156 showed significant homology to the enoyl-CoA hydratase/isomerase enzyme family.

157 sociated with the members of the 2-enoyl-CoA hydratase/isomerase enzyme superfamily are compared to s

158 active site of one member of the 2-enoyl-CoA hydratase/isomerase family, 4-chlorobenzoyl-CoA dehaloge

159 moderate sequence identity to members of the hydratase/isomerase superfamily of enzymes.

160 arity to FabA, but rather is a member of the hydratase/isomerase superfamily.

161 ication of a putative enoyl-coenzyme A (CoA) hydratase/isomerase that is required for synthesis of th

162 se family and that IBR10 resembles enoyl-CoA hydratases/isomerases.

163 at the level of the second enzyme, enoyl-CoA hydratase/L-3-hydroxyacyl-CoA dehydrogenase (L-PBE) of t

164 In this study, the function of enoyl-CoA hydratase/L-3-hydroxyacyl-CoA dehydrogenase (L-PBE), the

165 d transcription from a peroxisomal enoyl-CoA hydratase/l-3-hydroxyacyl-CoA dehydrogenase bifunctional

166 y of CBP and TRAP150, to the mouse enoyl-CoA hydratase/l-3-hydroxyacyl-CoA dehydrogenase gene promote

167 strates bind to the active site of enoyl-CoA hydratase, large spectral changes can be observed.

168 ence identity to a previously identified CHM hydratase (LigJ) fromSphingomonassp. SYK-6.

169 in this enzyme, suggesting that this nitrile hydratase, like the enzyme from Rhodococcus rhodochrous

170 mponent enzymes of TOC, long-chain enoyl-CoA hydratase, long-chain 3-hydroxyacyl-CoA dehydrogenase, a

171 wn that triosephosphate isomerase, aconitate hydratase, M-protein, nucleoside diphosphate kinase B, a

172 -oxidation mutants showed that the enoyl CoA-hydratase MAOC-1 serves an important role in ascaroside

173 (e.g., isocitrate dehydrogenase and fumarate hydratase) may enhance or mimic the effects of recurrent

174 Nitrile hydratase metalloenzymes are unique and important biocat

175 enzyme assigned as (3S)-methylglutaconyl-CoA hydratase (MGCH), which catalyzes the syn-hydration of (

176 In contrast, growth of a putative enoyl-CoA hydratase mutant (DeltaechA) was abolished on short-chai

177 vels in succinate dehydrogenase and fumarate hydratase-mutant tumors, were identified as potent Tet i

178 ing on nitrile metabolizing enzymes: nitrile hydratase (NHase) and amidase versus nitrilase activity.

179 Nitrile hydratase (NHase) catalyzes the hydration of nitriles to

180 Nitrile hydratase (NHase) is an iron-containing metalloenzyme th

181 Nitrile hydratase (NHase) is one of a growing number of enzymes

182 ive site of the non-heme iron enzyme nitrile hydratase (NHase) is studied using sulfur K-edge XAS and

183 catalytic role in the metalloenzyme nitrile hydratase (NHase), a reactive five-coordinate Co(III) th

184 o the catalytic mechanism of Fe-type nitrile hydratases (NHase), the pH and temperature dependence of

185 oth cysteine dioxygenases (CDOs) and nitrile hydratases (NHases), and yet the mechanisms by which sul

186 e a detailed catalytic mechanism for nitrile hydratases (NHases), the pH and temperature dependence o

187 rboxylase (gamma-RSD), and 4-oxalomesaconate hydratase (OMAH).

188 ism caused by deficient activity of fumarate hydratase, one of the constituent enzymes of the Krebs t

189 riments show that the MFP2 2-trans enoyl-CoA hydratase only exhibits activity against long chain (C18

190 on of the resonance Raman spectra of nitrile hydratase prepared at pH 7.3 and 9.0 shows a shift of in

191 The novel family of eukaryotic nitrile hydratases presented in this paper represents a promisin

192 ydratase (probably PrpD), 2-methylisocitrate hydratase (probably PrpD), and 2-methylisocitrate lyase

193 recapitulated by the incubation of fumarate hydratase-proficient cells with cell-permeable fumarate.

194 fer effect on the mechanism of the enoyl-CoA hydratase reaction is discussed.

195 quantitatively assessed from the K(m) in the hydratase reaction, 3 microM, and the K(i), 1.0 microM,

196 , is inactive in the dehydrogenation and the hydratase reactions.

197 xA (Rv1470), trxB (Rv1471), and an enoyl-coA hydratase (Rv1472), indicating a possible role for CtpD

198 Two nitrile hydratase sequences from an animal and a plant resolve i

199 Furthermore, the wild-type hydratase shows a bell-shaped pH dependence of the kcat/

200 , the acyl-CoA ligase SidI and the enoyl-CoA hydratase SidH, linking biosynthesis of mevalonate and T

201 ed to 3-hydroxypropionyl-CoA by acryloyl-CoA hydratase (SPO0147).

202 logy validates the modeling of the enoyl-CoA hydratase structure with the 4-(chlorobenzoyl)-CoA dehal

203 e, and is a fusion of beta and alpha nitrile hydratase subunits.

204 ncer, including the VHL, MET, FLCN, fumarate hydratase, succinate dehydrogenase, TSC1, TSC2, and TFE3

205 art of a consensus sequence in the enoyl-CoA hydratase superfamily, the results presented here provid

206 seven paralogues of the crotonase (enoyl CoA hydratase) superfamily.

207 th the strong sequence homology to enoyl-CoA hydratase support the intramolecular suprafacial transfe

208 s enzyme is a member of the class of nitrile hydratase that contains cobalt.

209 s, including a missense mutation in fumarate hydratase that controls variation in the mitochondrial u

210 In nitrile hydratase, the active-site Cys-SOH functions in both iro

211 Like the other cobalt-containing nitrile hydratases, this enzyme is relatively stable, maintainin

212 ompounds where ring-cleavage is achieved via hydratases, this lyase might represent a new ring-openin

213 Our assay uses fumarate hydratase to convert fumarate to malate and uses oxaloac

214 We have used the similarity of homoaconitate hydratase to isopropylmalate isomerase (serving in leuci

215 ed glutamate residues of rat liver enoyl-CoA hydratase to which Glu119 and Glu139 of the large alpha-

216 onate decarboxylase (4-OD) and vinylpyruvate hydratase (VPH) from Pseudomonas putida mt-2 form a comp

217 otonate tautomerase (4-OT) and vinylpyruvate hydratase (VPH) from the catechol meta-fission pathway a

218 alytically essential in homologous enoyl-CoA hydratases was also essential in CHY1.

219 a coli enolase (EC 4.2.1.11, phosphopyruvate hydratase), which is a component of the RNA degradosome,

220 nzyme activity of an iron-containing nitrile hydratase, which requires a catalytic alphaCys114-SOH in

221 vity changes caused by mutations in fumarate hydratase, which underlie the familial cancer predisposi

222 udes ACMSD and ACMSD-like decarboxylases and hydratases with diverse substrate specificities, many of

223 ease, respectively, in the kcat of enoyl-CoA hydratase without a significant change in the Km value o

224 y an acyl-CoA dehydrogenase and a subsequent hydratase yielding an intermediate with a tertiary hydro