ライフサイエンスコーパス: hydrogenase

1 dF is a scaffold protein, HydA1 is a natural hydrogenase).

2 ding an actinobacteria-type H2-uptake [NiFe]-hydrogenase.

3 , photosystem II, to the H2 evolving enzyme, hydrogenase.

4 of Desulfovibrio vulgaris Miyazaki F [NiFe]-hydrogenase.

5 te the proton reduction activity of a [NiFe] hydrogenase.

6 HypC residues relevant for the maturation of hydrogenase.

7 component of the catalytic H-cluster of FeFe hydrogenase.

8 ic electron transport chain and a plastidial hydrogenase.

9 hydrogen evolution activity of this class of hydrogenase.

10 n between flavodoxin/ferredoxin and the NiFe-hydrogenase.

11 itting electron flow from water oxidation to hydrogenase.

12 assembly of the catalytic H cluster of FeFe hydrogenase.

13 g the assembly line toward functional [NiFe]-hydrogenase.

14 O, thereby providing the carbonyl ligand for hydrogenase.

15 of nickel-containing enzymes such as [NiFe]-hydrogenase.

16 f glycine ends up in the CO ligand of [NiFe]-hydrogenase.

17 it elusive, catalytic intermediate of [NiFe]-hydrogenases.

18 ession but decreased expression of all other hydrogenases.

19 the well-studied dinuclear [FeFe] and [NiFe] hydrogenases.

20 ion emulate components of the active site of hydrogenases.

21 E. coli hydrogenase 1 (Hyd-1) is adsorbed on a high surface-area

22 es, that the Ni-C to Ni-L interconversion in Hydrogenase-1 (Hyd-1) from Escherichia coli is a pH-depe

23 Hydrogenase-1 (Hyd1) (MSMEG_2262-2263) is well-adapted t

24 ide reacts rapidly with [NiFe]-hydrogenases (hydrogenase-1 and hydrogenase-2 from Escherichia coli) u

25 86% of the H2O produced by Escherichia coli hydrogenase-1 exposed to a mixture of 90% H2 and 10% O2

26 electron entry/exit site in Escherichia coli hydrogenase-1 is shown to play a vital role in tuning bi

27 Escherichia coli uptake hydrogenase 2 (Hyd-2) catalyzes the reversible oxidation

28 with [NiFe]-hydrogenases (hydrogenase-1 and hydrogenase-2 from Escherichia coli) under mild oxidizin

29 y that allows synthesizing functional [NiFe]-hydrogenase-2 of Escherichia coli from purified componen

30 he biosynthesis of the active site of [NiFe]-hydrogenases, a family of H2-activating enzymes.

31 Hydrogenase accessory proteins from bacteria-synthesizin

32 t the organometallic component of the [FeFe]-hydrogenase active site (the H-cluster).

33 terfly shape of the 2Fe2S core of the [FeFe]-hydrogenase active site but with nitrosyl rather than ca

34 anting Mu(.) into three models of the [FeFe]-hydrogenase active site we have been able to detect key

35 g in electrocatalysts inspired by the [NiFe]-hydrogenase active site.

36 This reflects either that H/D exchange at hydrogenase active sites is rapid compared to the rate o

37 As nominal models of hydrogenase active sites, these bimetallics feature two

38 This highlights the role of hydrogenase activity and PSI-CEF in the ecological succe

39 locality in the chloroplast preserves [FeFe]-hydrogenase activity and supports continuous hydrogen pr

40 The mutant had no uptake hydrogenase activity but had increased bidirectional hyd

41 cellular compartments, including detectable hydrogenase activity in Mastigamoeba cytosol and mitocho

42 This second assay measures the remaining hydrogenase activity in periodic samples taken from the

43 At light onset, hydrogenase activity sustains a linear electron flow fro

44 nases, rather than due to the sensitivity of hydrogenase activity to oxygen.

45 e mutant enzyme displayed severely decreased hydrogenase activity.

46 a a periplasmic formate dehydrogenase and/or hydrogenase, allowing energetic coupling to hydrogenotro

47 s as the corresponding cofactors in standard hydrogenases, although their redox potentials are higher

48 H2 PAD-enabled discovery of hydrogenase and FNR mutants that enhance biological H2 p

49 of S. aciditrophicus and S. wolfei had both hydrogenase and formate dehydrogenase activities.

50 S. aciditrophicus expressed multiple hydrogenase and formate dehydrogenase genes during syntr

51 Both organisms contain multiple hydrogenase and formate dehydrogenase genes, but lack ge

52 A putative monomeric [Fe-Fe] hydrogenase and Hmc (high-molecular-weight-cytochrome c3

53 The instability of [Fe-Fe]-hydrogenase and its synthetic models under aerobic condi

54 MBH is encoded by a 14-gene operon with both hydrogenase and Na(+)/H(+) antiporter modules.

55 ch is required for Ni(II) delivery to [NiFe]-hydrogenase and participates in urease maturation.

56 g postulates a direct link between CrPFO and hydrogenase and provides new opportunities to better stu

57 enes involved in the SurR regulon, including hydrogenase and related S(0) -responsive genes.

58 Transcripts of soluble hydrogenases and ferredoxins from Acetobacterium and hyd

59 I possesses electron transfer flavoproteins, hydrogenases and formate dehydrogenases essential for sy

60 teria, hydrogen evolution is associated with hydrogenases and nitrogenase, making these enzymes inter

61 Enzymes such as the hydrogenases and nitrogenases are also proposed to invol

62 ntext of the function of the active sites of hydrogenases and nitrogenases.

63 for exploring S-oxygenated intermediates in hydrogenases and similar enzymes.

64 ones, aldehydes, imines, and carbon dioxide, hydrogenases and their model complexes, and palladium ca

65 ures resembling those of O2-sensitive [NiFe] hydrogenases and/or oxidatively damaged protein.

66 of hydride complexes found in nature (e.g., hydrogenases) and in industry (e.g., catalysis and hydro

67 ct metabolic interactions (e.g., periplasmic hydrogenases) and the ratio shift in electron carriers u

68 Nitrogenase, [FeFe]-hydrogenase, and [Fe]-hydrogenase enzymes perform cataly

69 oCbl)-dependent methylmalonyl-CoA mutase and hydrogenase, and thus have both medical and biofuel deve

70 ne gene families involved in photosynthesis, hydrogenases, and proteins involved in defense from envi

71 nal data obtained with various NiFe and FeFe hydrogenases, and we illustrate how the presence of an i

72 oxygen tolerance in soluble, group 3 [NiFe]-hydrogenases, and we present a model integrating both el

73 onic properties of the active site of [NiFe]-hydrogenases are crucial for efficient H2 binding and cl

74 [NiFe] hydrogenases are key enzymes for the energy and redox me

75 [NiFe] hydrogenases are metalloenzymes that catalyze the revers

76 Consequently, all hydrogenases are metalloenzymes that contain at least on

77 Oxygen-tolerant [NiFe] hydrogenases are metalloenzymes that represent valuable

78 Hydrogenases are nature's key catalysts involved in both

79 Hydrogenases are oxygen-sensitive enzymes that catalyze

80 [NiFe]-hydrogenases are redox enzymes composed of a large subun

81 generally accepted that cyanobacterial NiFe-hydrogenases are reduced by NAD(P)H.

82 [FeFe]-hydrogenases are the best natural hydrogen-producing enz

83 FeFe hydrogenases are the most efficient H2-producing enzymes

84 Because bidirectional hydrogenases are widespread in aquatic nutrient-rich env

85 active sites of a number of enzymes (such as hydrogenases), are promising therapeutic agents, and hav

86 netic optimization of an artificial transfer hydrogenase (ATHase hereafter), [(eta(5)-Cp*)Ir(pico)Cl]

87 lds an NAD(P)H-dependent artificial transfer hydrogenase (ATHase).

88 n, thus overcoming the O2 sensitivity of the hydrogenase, but its activity is low.

89 cs is recognized for many enzymes, including hydrogenases, but is largely neglected in designing synt

90 he absence of S degrees and have up to seven hydrogenases, but their combined physiological roles are

91 ear active site of an oxygen-tolerant [NiFe] hydrogenase by probing the metal-ligand modes of both th

92 e structure of the active site of the [FeFe] hydrogenases by assembling the active enzyme with a vers

93 Take a breath: An oxygen-tolerant hydrogenase can be employed with a dye in a photocatalyt

94 o study has ever unambiguously proven that a hydrogenase can oxidize this trace gas.

95 Active [FeFe] hydrogenases can be obtained by expressing the unmaturat

96 sid provides stability and protection to the hydrogenase cargo.

97 In FeFe hydrogenases, catalysis occurs at the H cluster, a metal

98 The [FeFe]-hydrogenase catalytic site H cluster is a complex iron s

99 FeFe hydrogenases catalyze H2 oxidation and formation at an i

100 [FeFe] hydrogenases catalyze proton reduction and hydrogen oxid

101 [FeFe] hydrogenases catalyze rapid H2 production but are highly

102 Hydrogenases catalyze the redox interconversion of proto

103 The [FeFe]-hydrogenases catalyze the reversible activation of molec

104 [NiFe] hydrogenases catalyze the reversible cleavage of hydroge

105 [Fe]-Hydrogenase catalyzes the hydrogenation of a biological

106 ins, is responsible for the synthesis of the hydrogenase CO and CN(-) ligands from tyrosine-derived d

107 gands as well as dithiomethylamine; the [Fe]-hydrogenase cofactor has CO and guanylylpyridinol ligand

108 complex is shown to comprise HycE (a [NiFe] hydrogenase component termed Hyd-3), FdhF (the molybdenu

109 viding rate constants insensitive to initial hydrogenase concentration.

110 The catalytic H-cluster of [FeFe]-hydrogenase consists of a [4Fe-4S] subcluster ([4Fe-4S]H

111 [FeFe]-Hydrogenases contain a H2-converting cofactor (H-cluster

112 These results may apply to all [FeFe] hydrogenases containing accessory clusters.

113 tral carbon, whereas the H-cluster of [FeFe]-hydrogenase contains a 2Fe subcluster coordinated by cya

114 The active site of [FeFe] hydrogenase contains a catalytic binuclear iron subsite

115 Hydrogenases control the H2-related metabolism in many m

116 and activation in the active site of [NiFe]-hydrogenases could be exploited in the design of novel b

117 Hydrogenases couple electrochemical potential to the rev

118 ing the Clostridium pasteurianum (Cp) [FeFe] hydrogenase, CpI, we detected significant rates of direc

119 -cluster of Chlamydomonas reinhardtii [FeFe]-hydrogenase (CrHydA1) affects the H-cluster using electr

120 Experiments were carried out on two [FeFe]-hydrogenases, CrHydA1 from the green alga Chlamydomonas

121 In the case of rod-shaped Cpl hydrogenase crystals, only five crystals and about 30 mi

122 ression of HYDA and the specific activity of hydrogenase demonstrate that C. vulgaris YSL01 and YSL16

123 Mono-iron hydrogenase was the third type of hydrogenase discovered.

124 Attached to an electrode, hydrogenases display reversible electrocatalytic behavio

125 urthermore, PfSHI, like other group 3 [NiFe]-hydrogenases, does not possess the proximal [4Fe3S] clus

126 is demonstrated for the characterization of hydrogenase during catalytic turnover.

127 We further reveal that group 2a [NiFe] hydrogenases (e.g., Hyd1) can contribute to this process

128 ious studies that have linked group 5 [NiFe] hydrogenases (e.g., Hyd2) to the oxidation of tropospher

129 microelectrodes were modified with a [NiFe]-hydrogenase embedded in a viologen-modified redox hydrog

130 supporting CO2 fixation, as well as a [NiFe] hydrogenase-encoding gene cluster in H2 oxidation.

131 ional model of the active site of the [FeFe] hydrogenase enzyme.

132 ions, including the oxidation of hydrogen by hydrogenase enzymes and ionic hydrogenation of organic c

133 highly express genes encoding group 1 Ni, Fe hydrogenase enzymes for H(2) oxidation.

134 Hydrogenase enzymes in nature use hydrogen as a fuel, bu

135 Nitrogenase, [FeFe]-hydrogenase, and [Fe]-hydrogenase enzymes perform catalysis at metal cofactors

136 Hydrogenase enzymes use Ni and Fe to oxidize H2 at high

137 how the H-H bond is oxidized or generated in hydrogenase enzymes.

138 tion/oxidation at rates approaching those of hydrogenase enzymes.

139 The immobilized hydrogenase exhibits activity on Si attributable to a bi

140 Hydrogenase expression and activity increases in carbon-

141 Hydrogenases, ferredoxins, and ferredoxin-NADP(+) reduct

142 llent integration of both photosystem II and hydrogenase for performing the anodic and cathodic half-

143 ecause they may negatively impact the use of hydrogenase for the photoproduction of H2.

144 nickel-containing enzymes, urease and [NiFe]-hydrogenase, for efficient colonization of the human gas

145 ases and ferredoxins from Acetobacterium and hydrogenases, formate dehydrogenase, and cytochromes of

146 c methanogenesis: a coenzyme F(420)-reducing hydrogenase (FrcA) and an iron sulfur protein (MvrD).

147 36 A resolution of the 1.2 MDa F420-reducing hydrogenase (Frh) from methanogenic archaea from only 32

148 screened 48 amino acid substitutions of the hydrogenase from A. macleodii "deep ecotype", to underst

149 Using the hydrogenase from Alteromonas macleodii "deep ecotype" as

150 FTIR spectro-electrochemistry on the [FeFe] hydrogenase from Chlamydomonas reinhardtii (CrHydA1) at

151 t FTIR electrochemical studies of the [FeFe] hydrogenase from Chlamydomonas reinhardtii, CrHydA1, mat

152 f a hydride-bound state (Hhyd) of the [FeFe]-hydrogenase from Chlamydomonas reinhardtii.

153 An [FeFe]-hydrogenase from Clostridium pasteurianum, CpI, is a mod

154 The [FeFe] hydrogenase from Desulfovibrio desulfuricans is exceptio

155 The heterodimeric [NiFe] hydrogenase from Desulfovibrio fructosovorans catalyzes

156 2 N(Gly) 2 )2 ](2+) complex with the [NiFe]-hydrogenase from Desulfovibrio vulgaris immobilized on a

157 by comparing the data obtained with the FeFe hydrogenases from Chlamydomonas reinhardtii and Clostrid

158 We show that the two most studied FeFe hydrogenases, from Chlamydomonas reinhardtii and Clostri

159 The results show promise as a hydrogenase functional mimic derived from a biomolecule.

160 uration (100 s), thus indicating that [FeFe]-hydrogenase functions as an immediate sink for surplus e

161 and YSL16) upregulate the expression of the hydrogenase gene (HYDA) and simultaneously produce hydro

162 Two hydrogenase genes, hydA1 and hydA2, were more highly exp

163 The six-iron cofactor of [FeFe]-hydrogenases (H-cluster) is the most efficient H2-formin

164 In FeFe hydrogenases, H2 oxidation occurs at the H-cluster, and

165 cofactors and hydride substrate into [NiFe]-hydrogenase (H2ase) active site models.

166 riven H2 production in water with a [NiFeSe]-hydrogenase (H2ase) and a bioinspired synthetic nickel c

167 tron transfer rates increase with increasing hydrogenase (H2ase) enzyme activity.

168 hydrogenation of fumarate to succinate or a hydrogenase (H2ase) for reduction of protons to H2.

169 The [NiFe] hydrogenase (H2ase) has been characterized in the Ni-R s

170 No strong phenotype of mutants lacking the hydrogenase has been found.

171 In contrast, a second hydrogenase has more abundant transcripts in background

172 The active site of hydrogenases has been a source of inspiration for the de

173 espiratory chains and to subunits of several hydrogenases has raised interest in the evolutionary pat

174 While many synthetic models of [Fe]-hydrogenase have been prepared, none yet are capable of

175 f hydrogen oxidation and evolution by [FeFe]-hydrogenases have been investigated by electrochemical i

176 Certain bacterial enzymes, the diiron hydrogenases, have turnover numbers for hydrogen product

177 [Fe] hydrogenase (Hmd) catalyzes the heterolytic splitting of

178 ase activity but had increased bidirectional hydrogenase (Hox) activity.

179 ous cyanobacterium that expresses the uptake hydrogenase HupSL in heterocysts under nitrogen-fixing c

180 w that DosR tightly regulates the two [NiFe]-hydrogenases: Hyd3 (MSMEG_3931-3928) and Hyd2 (MSMEG_271

181 tobutylicum 2[4Fe-4S]-ferredoxin and [Fe-Fe]-hydrogenase HYDA.

182 The [FeFe] hydrogenase HydA1 from Chlamydomonas reinhardtii has bee

183 s then used for the maturation of the [FeFe] hydrogenase HydA1 from Chlamydomonas reinhardtii, to yie

184 the CO/CN(-) stretching vibrations in [FeFe]-hydrogenase HYDA1 from Chlamydomonas reinhardtii.

185 degradation, the highly O2-sensitive [FeFe]-hydrogenase HydA1 from the green algae Chlamydomonas rei

186 H(2) is generated mostly by the [Fe-Fe]-hydrogenase HYDA1, which uses plant type ferredoxin PETF

187 ained, besides a ferredoxin-dependent [FeFe]-hydrogenase (HydA2), a ferredoxin- and NAD-dependent ele

188 nd NAD-dependent electron-bifurcating [FeFe]-hydrogenase (HydABC) that couples the endergonic formati

189 complexes formate dehydrogenase (FdhABC) and hydrogenase (HydABCD) as well as the transcription of th

190 Cyanide reacts rapidly with [NiFe]-hydrogenases (hydrogenase-1 and hydrogenase-2 from Esche

191 an oxygen-tolerant, group 3, soluble [NiFe]-hydrogenase: hydrogenase I from Pyrococcus furiosus (PfS

192 ystals and Clostridium acetobutylicum [FeFe] hydrogenase I (CaI) enabled light-driven control of elec

193 mplexes of CdS nanorods (CdS NRs) and [FeFe]-hydrogenase I from Clostridium acetobutylicum (CaI).

194 lerant, group 3, soluble [NiFe]-hydrogenase: hydrogenase I from Pyrococcus furiosus (PfSHI), which gr

195 utative proton donor E17 to Q in the soluble hydrogenase I from Pyrococcus furiosus using site direct

196 ccessory proteins from bacteria-synthesizing hydrogenase in the presence of oxygen include HupK, a sc

197 strongly support that the bidirectional NiFe-hydrogenases in cyanobacteria function as electron sinks

198 tonia eutropha, which produces active [NiFe]-hydrogenases in the presence of O2, employs the auxiliar

199 Here, we show which hydrogenases in Thermococcus paralvinellae are affected

200 igate the progression of O2-dependent [FeFe]-hydrogenase inactivation and the process of H cluster de

201 Hydrogenase inactivation is measured during H2 productio

202 ot interfere with measurement of first order hydrogenase inactivation, providing rate constants insen

203 Recent studies on O2-tolerant membrane-bound hydrogenases indicate that the mechanism of O2 tolerance

204 Methanospirillum hungatei were inhibited by hydrogenase inhibitors (cyanide and carbon monoxide), bu

205 Hydrogenases interconvert H2 and protons at high rates a

206 pressures, HydS could be a H2-sensing [FeFe]-hydrogenase involved in the regulation of their biosynth

207 of synthetic model complexes of the [Fe-Fe] hydrogenase is investigated, and a dominant role of the

208 Until recently, it was thought that hydrogenase is not active in air-grown microalgal cells.

209 An oxygen-tolerant respiratory [NiFe]-hydrogenase is proven to be a four-electron hydrogen/oxy

210 allic H-cluster at the active site of [FeFe]-hydrogenases is synthesized by three accessory proteins,

211 The light-induced Ni-L state of [NiFe] hydrogenases is well suited to investigate the identity

212 e inhibitor of hydrogen production by [FeFe]-hydrogenases, is used to identify the point in the catal

213 monas reinhardtii is catalyzed by two [FeFe]-hydrogenase isoforms, HydA1 and HydA2, both irreversibly

214 bly of the complex 6Fe active site of [FeFe]-hydrogenases (known as the H-cluster) from its precursor

215 n with a moderate sequence similarity to the hydrogenase large subunit and proposed to participate as

216 way, including two homologs of fdhF encoding hydrogenase-linked formate dehydrogenases (FDHH ) and al

217 Using the hydrogenase maturase HydE from Thermotoga maritima as a

218 lternative pathways in a double mutant pgrl1 hydrogenase maturation factor G-2 is detrimental for pho

219 onse to reactive oxygen species, and FDX5 in hydrogenase maturation.

220 Soil organisms harboring high-affinity hydrogenases may be especially competitive, given that t

221 The membrane-bound [NiFe] hydrogenase (MBH) supports growth of Ralstonia eutropha

222 ing energy from a respiratory membrane-bound hydrogenase (MBH).

223 in the O2-tolerance of membrane-bound [NiFe]-hydrogenase (MBH).

224 oluble subcomplexes of membrane-bound [NiFe]-hydrogenases (MBH) have been extensively studied for app

225 membrane-associated, oxygen-dependent [NiFe] hydrogenases mediate this process.

226 he adjacent Fe-S centers in this O2-tolerant hydrogenase might also be a contributory factor, impedin

227 ble information for the design of artificial hydrogenase mimics.

228 ld provide important clues for the design of hydrogenase mutants with increased resistance to oxidati

229 his precise, solution phase assay for [FeFe] hydrogenase O2 sensitivity and the insights we provide c

230 doxin directly reduce the bidirectional NiFe-hydrogenase of Synechocystis sp. PCC 6803 in vitro.

231 Herein, we report the integration of a hydrogenase on a TiO2 -coated p-Si photocathode for the

232 state known as Ni-L, observed in other NiFe hydrogenases only under illumination or at cryogenic tem

233 RNA-Seq showed consistent expression of six hydrogenase operons with and without added H2 .

234 ration of the chloroplastic oxygen-sensitive hydrogenases or in Proton-Gradient Regulation-Like1 (PGR

235 e ligands in the assembled cluster of [FeFe] hydrogenase originate from exogenous l-tyrosine.

236 ages, most of the electrons delivered to the hydrogenase originate from water oxidation by PSII, (ii)

237 Metronidazole inhibits the ferredoxin/hydrogenase pathway of fermentative eukaryotic H2 produc

238 nts, or by the progressive activation of the hydrogenase pathway, which provides an electron transfer

239 The p-Si|TiO2 |hydrogenase photocathode displays visible-light driven p

240 e of O2, employs the auxiliary protein HypX (hydrogenase pleiotropic maturation X) for CO ligand form

241 sets the stage for optimizing the design of hydrogenase-polymer films, and for expanding this strate

242 ime-dependent distribution of species in the hydrogenase/polymer film, using measured or estimated va

243 erial by expressing and maturing the EcHyd-1 hydrogenase prior to expression of the P22 coat protein,

244 ntrolled hydration of three different [FeFe]-hydrogenase proteins produced 8 Hox and 16 Hox-CO specie

245 Mediated by a hydrogenase, protons reoxidize the fully reduced flavodo

246 e nor evolution of the gas was detected in a hydrogenase quadruple-mutant strain containing deletions

247 analysis of a series of DFT models of [NiFe]-hydrogenases, ranging from minimal NiFe clusters to very

248 etween ferredoxin-NADP(+) oxidoreductase and hydrogenases, rather than due to the sensitivity of hydr

249 We furthermore found that the hydrogenase receives its electrons via pyruvate:flavodox

250 show that the entire pool of cellular [FeFe]-hydrogenase remains active in air-grown cells due to eff

251 bly of the active site [FeFe] unit of [FeFe]-hydrogenases require at least three maturases.

252 ward the discovery of the O2-tolerant [FeFe] hydrogenases required for photosynthetic, biological H2

253 Alignment of hydrogenase sequences from sequence databases revealed m

254 ne positions found in a broad survey of NiFe hydrogenase sequences.

255 ydrogen-producing and oxygen-tolerant [NiFe]-hydrogenase, sequestered within the capsid of the bacter

256 The soluble NAD(+)-reducing [NiFe] hydrogenase (SH) from Ralstonia eutropha couples the rev

257 acity of the soluble, NAD(+)-reducing [NiFe]-hydrogenase (SH) from Ralstonia eutropha H16.

258 oxygen, and we propose that this new type of hydrogenase should be referred to as oxygen-resilient.

259 mally ligating the three FeS clusters in the hydrogenase small subunit.

260 ecifically with VhuD in the absence of other hydrogenase subunits.

261 um Rhizobium leguminosarum contains a single hydrogenase system that can be expressed under two diffe

262 an accessory protein conserved in all [NiFe] hydrogenase systems and involved in the synthesis and tr

263 Hyd-2 is an unusual heterotetrameric [NiFe]-hydrogenase that lacks a typical cytochrome b membrane a

264 A different hydrogenase, the hydrogen-evolving Hyc enzyme, removes e

265 aches are used to elucidate the mechanism of hydrogenases, the enzymes that oxidize or produce H2.

266 Some of us have recently demonstrated that hydrogenases, the fragile but very efficient biological

267 In [FeFe]-hydrogenases, the H-cluster cofactor includes a diiron s

268 The active site of [FeFe] hydrogenases, the H-cluster, consists of a [4Fe-4S] clus

269 Despite extensive studies on [NiFe]-hydrogenases, the mechanism by which these enzymes produ

270 f H2 activation has been proposed for [FeFe]-hydrogenases, the structural and biophysical properties

271 s from approximately 5.8 for the Hox form of hydrogenase to <2 for the CO-inhibited form.

272 ode with a bioanode that utilizes the enzyme hydrogenase to oxidize molecular hydrogen (H2 ) results

273 The assignment of 156 hydrogenases to 90 different organisms suggests that H2

274 te that it has a unique ability among [NiFe] hydrogenases to catalyze production of H2 even at high p

275 For example, bacteria and archaea use [NiFe]-hydrogenases to catalyze the uptake and release of molec

276 Hydrogenases use complex metal cofactors to catalyze the

277 nthetic approach by which to model mono-iron hydrogenase using an anthracene framework, which support

278 unprecedented functional model for the [Fe] hydrogenase, using a Lewis acidic imidazolinium salt as

279 se (FNR) to transfer electrons from NADPH to hydrogenase via ferredoxins (Fd).

280 Under these conditions the hydrogenase was found to be essential.

281 Mono-iron hydrogenase was the third type of hydrogenase discovered

282 far, biosynthesis of the CO ligand in [NiFe]-hydrogenases was unknown.

283 hemical measurements of the turnover rate of hydrogenase, we could resolve the first steps of the inh

284 cycling by a soluble NAD(+) -reducing [NiFe] hydrogenase, we herein present the first bioinspired het

285 effects of FeS cluster attachment in [NiFe] hydrogenase, we undertook a study to substitute all 12 a

286 omly mutated Clostridium pasteurianum [FeFe] hydrogenases, we found a mutant with nearly 3-fold highe

287 ding for ATP synthase, biosynthesis, and Hym hydrogenase were down-regulated during C2H2 inhibition,

288 f a so far unknown type of NAD(P)H-accepting hydrogenase, which is expressed in the presence, but not

289 functional mimic of the active site of [Fe]-hydrogenase, which was developed based on a mechanistic

290 Hydrogenases, which catalyze the reversible reduction of

291 s populations appears to be linked with NiFe hydrogenases, which combined with high levels of H2 in m

292 roperties of oxygen-tolerant, group 1 [NiFe]-hydrogenases, which form a single state upon reaction wi

293 etabolism of many anaerobes relies on [NiFe]-hydrogenases, whose characterization when bound to subst

294 gene, which is predicted to encode an [FeFe]-hydrogenase with a C-terminal PAS domain.

295 ic characterization of a CO-inhibited [FeFe] hydrogenase with a selectively (57)Fe-labeled binuclear

296 reaction of Chlamydomonas reinhardtii [FeFe]-hydrogenase with formaldehyde using pulsed-EPR technique

297 istic understanding of catalysis in a [NiFe] hydrogenase with implications in enzymatic proton-couple

298 ive electron flow from photosystem II to the hydrogenase with the production of H2 and O2 being in th

299 The mechanism of reaction of FeFe hydrogenases with oxygen has been debated.

300 the H2 production phase, indicating that the hydrogenase withdraws electrons from the plastoquinone p