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1 protein that is monomeric, well-folded, and hyperthermophilic.
2 study demonstrates coenzyme engineering of a hyperthermophilic 6PGDH and its application to high-temp
3 s islandicus rod-shaped virus 2) infects the hyperthermophilic acidophile Sulfolobus islandicus, whic
4 aracterized an archaeal antioxidant from the hyperthermophilic acidophile Sulfolobus solfataricus.
5 l mobilities differ between a mesophilic and hyperthermophilic adenylate kinase, but are strikingly s
11 ogue (TP0823) for neelaredoxin, an enzyme of hyperthermophilic and sulfate-reducing anaerobes shown t
12 nt comparison of ECAK dynamics with those of hyperthermophilic Aquifex aeolicus AK (AAAK), our result
13 ) accumulates as a compatible solute in many hyperthermophilic archaea (e.g., Archaeoglobus fulgidus)
14 riginated in an extreme environment, such as hyperthermophilic archaea (Pyrococcus furiosus), are sig
15 RNA-encoding DNA analysis places many of the hyperthermophilic Archaea (species with an optimum growt
16 f structures and complete genomes of several hyperthermophilic archaea and bacteria revealed that org
17 bilizes tRNAs from thermophilic bacteria and hyperthermophilic archaea and is required for growth at
18 interaction with uracil is not restricted to hyperthermophilic archaea and that the polymerase from m
22 In particular, the approaches employed by hyperthermophilic archaea have been a general source of
23 f the DNA replication-associated proteins of hyperthermophilic archaea have yielded considerable insi
24 Inositol monophosphatase (EC 3.1.3.25) in hyperthermophilic archaea is thought to play a role in t
28 ficity and binding mechanism of MCM from the hyperthermophilic Archaea Sulfolobus solfataricus on var
31 quences (ISs) are abundant and widespread in hyperthermophilic archaea, but few experimental studies
32 e that the intracellular proteins of certain hyperthermophilic archaea, especially the crenarchaea Py
35 d over a contiguous 16 kb region between two hyperthermophilic Archaea, Pyrococcus furiosus and Therm
36 esponses have been of particular interest in hyperthermophilic archaea, since these microbes live und
37 s of recombination involving short ssDNAs in hyperthermophilic archaea, we evaluated oligonucleotide-
43 7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataric
45 Moreover, this latest example of a split hyperthermophilic archaeal DNA polymerase further illust
46 els for dNTP, ddNTP, and acyNTP selection by hyperthermophilic archaeal DNA polymerases to rationaliz
47 the parameters for dNTP incorporation by the hyperthermophilic archaeal Family B Vent DNA polymerase
48 trand RNA viruses that probably replicate in hyperthermophilic archaeal hosts and are highly divergen
50 Analysis of the genome sequence of the small hyperthermophilic archaeal parasite Nanoarchaeum equitan
52 first structure of a catalytic domain from a hyperthermophilic archaeal viral integrase reveals a min
56 a voltage-dependent K+ (K(V)) channel from a hyperthermophilic archaebacterium from an oceanic therma
57 structure of adenylosuccinate lyase from the hyperthermophilic archaebacterium Pyrobaculum aerophilum
58 is work, we characterize the enzyme from the hyperthermophilic archaebacterium Pyrococcus furiosus.
61 m chain alcohol dehydrogenase (ADH) from the hyperthermophilic archaeon Aeropyrum pernix has been sol
62 he Aeropyrum coil-shaped virus (ACV), of the hyperthermophilic archaeon Aeropyrum pernix, with a viri
64 A new carboxyl esterase, AF-Est2, from the hyperthermophilic archaeon Archaeoglobus fulgidus has be
66 structure of the 104 residue SRP19 from the hyperthermophilic archaeon Archaeoglobus fulgidus, desig
69 ctures, to our knowledge, of an ACD from the hyperthermophilic archaeon Candidatus Korachaeum cryptof
71 Sulfolobus acidocaldarius is so far the only hyperthermophilic archaeon in which genetic recombinatio
81 an endonuclease III homolog, PaNth, from the hyperthermophilic archaeon Pyrobaculum aerophilum, whose
82 ation of a putative DNA glycosylase from the hyperthermophilic archaeon Pyrobaculum aerophilum, whose
87 transcarbamoylase (OTCase) from the deep sea hyperthermophilic archaeon Pyrococcus abyssi demonstrate
88 ned the solution structure of RPP21 from the hyperthermophilic archaeon Pyrococcus furiosus ( Pfu) us
89 ngle cubane cluster ferredoxin (Fd) from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) have
90 ngle cubane cluster ferredoxin (Fd) from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) poss
91 es from Escherichia coli (EcMetAP-I) and the hyperthermophilic archaeon Pyrococcus furiosus (PfMetAP-
92 ng for the methionyl aminopeptidase from the hyperthermophilic archaeon Pyrococcus furiosus (PfMetAP-
93 tic studies were conducted on the POP of the hyperthermophilic archaeon Pyrococcus furiosus (Pfu) 85
94 protons of perdeuterated rubredoxin from the hyperthermophilic archaeon Pyrococcus furiosus and the m
96 ylase was identified in cell extracts of the hyperthermophilic archaeon Pyrococcus furiosus by its ab
98 shed membrane preparations from cells of the hyperthermophilic archaeon Pyrococcus furiosus contain h
103 The cytoplasmic hydrogenase (SHI) of the hyperthermophilic archaeon Pyrococcus furiosus is an NAD
107 a tungstopterin-containing protein from the hyperthermophilic archaeon Pyrococcus furiosus, have bee
114 A intein located in the ATPase domain of the hyperthermophilic archaeon Pyrococcus horikoshii is stro
116 domain of life, with the discovery that the hyperthermophilic archaeon Sulfolobus has three replicat
117 II chaperonins known as rosettasomes in the hyperthermophilic archaeon Sulfolobus shibatae, are not
121 ce of a global gene regulatory system in the hyperthermophilic archaeon Sulfolobus solfataricus is de
122 ral modules of the homomultimeric MCM of the hyperthermophilic archaeon Sulfolobus solfataricus.
123 in chromatin structure and regulation in the hyperthermophilic archaeon Sulfolobus solfataricus.
124 noncatalytic subunit, denoted PriX, from the hyperthermophilic archaeon Sulfolobus solfataricus.
125 eavage of mRNA from an invading virus in the hyperthermophilic archaeon Sulfolobus solfataricus.
126 gh mutagenesis of the Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus.
127 gh mutagenesis of the Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus; Sso7
129 structure of TIM from Thermoproteus tenax, a hyperthermophilic archaeon that has an optimum growth te
130 cture of the ribosomal protein L30e from the hyperthermophilic archaeon Thermococcus celer determined
131 that ribonucleotides are incorporated by the hyperthermophilic archaeon Thermococcus kodakarensis bot
135 ted Box C/D RNAs from Pyrococcus furiosus, a hyperthermophilic archaeon, into the nuclei of oocytes f
139 ily DNA replication polymerase (Dpo1) in the hyperthermophilic archaeon, Sulfolobus solfataricus, is
145 aracterized an unusual type IA enzyme from a hyperthermophilic archaeum, Nanoarchaeum equitans, which
148 methanarchaeon, Methanococcus jannaschii, a hyperthermophilic, autotrophic, and strictly hydrogenotr
151 existence of peptide-based quorum sensing in hyperthermophilic bacteria and indicate that cellular co
152 .4 A resolution, of the class I BPL from the hyperthermophilic bacteria Aquifex aeolicus (AaBPL) in i
153 ins taken from mesophilic, thermophilic, and hyperthermophilic bacteria are studied using these metho
155 Our results explain the root location of hyperthermophilic bacteria in the phylogenetic tree for
159 onate-8-phosphate synthase (KDO8PS) from the hyperthermophilic bacterium Aquifex aeolicus differs fro
161 tural features of the NtrC1 protein from the hyperthermophilic bacterium Aquifex aeolicus suggested t
162 An NAD(+)-dependent DNA ligase from the hyperthermophilic bacterium Aquifex aeolicus was cloned,
163 rt the crystal structures of GatCAB from the hyperthermophilic bacterium Aquifex aeolicus, complexed
164 he crystal structure of a homologue from the hyperthermophilic bacterium Aquifex aeolicus, that share
170 unknown type of protein-only RNase P in the hyperthermophilic bacterium Aquifex aeolicus: Without an
171 is of the crystal structure of MurI from the hyperthermophilic bacterium Aquifex pyrophilus, we perfo
172 us ribose-binding protein, isolated from the hyperthermophilic bacterium Thermoanaerobacter tengconge
173 RAP-PBP (open reading frame tm0322) from the hyperthermophilic bacterium Thermotoga maritima (TM0322)
174 hosphogluconate dehydrogenase (6PGDH) from a hyperthermophilic bacterium Thermotoga maritima from its
178 ide expression patterns during growth of the hyperthermophilic bacterium Thermotoga maritima on 14 mo
179 The biochemical behavior of RNase III of the hyperthermophilic bacterium Thermotoga maritima was anal
180 The structure of RNase P protein from the hyperthermophilic bacterium Thermotoga maritima was dete
181 of proteins from one specific organism, the hyperthermophilic bacterium Thermotoga maritima, and tho
188 tandemly repeated GTP-binding domains from a hyperthermophilic bacterium, Thermotoga maritima, was cl
194 nstrate that the Thermoproteales, a clade of hyperthermophilic Crenarchaea, lack a canonical SSB.
197 es or genes that are first in operons in the hyperthermophilic crenarchaeon P. aerophilum proceeds mo
198 tion crystal structure of SurEalpha from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum (P
199 and a PCNA homolog (Pa-PCNA1), both from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum (T
200 ionine beta-synthase domain protein from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum.
201 h10b, a member of the Sac10b family from the hyperthermophilic crenarchaeon Sulfolobus shibatae, bind
202 erial-like DnaG primase contained within the hyperthermophilic crenarchaeon Sulfolobus solfataricus (
204 erase-Like Lactonases (PLLs) family from the hyperthermophilic crenarchaeon Vulcanisaeta moutnovskia
206 ea, and an activity has been observed in the hyperthermophilic crenarchaeote Sulfolobus solfataricus.
208 architecture of this protein is uncommon for hyperthermophilic endoglucanases, and two of the four do
210 or directly assaying the activity of another hyperthermophilic enzyme, 1,4-beta-D-glucan glucohydrola
212 ut the substrate specificity of two specific hyperthermophilic enzymes and the first test of some nat
223 p in CoM biosynthesis, was identified in the hyperthermophilic euryarchaeon Methanococcus jannaschii.
226 nd molecular screens, although abundances of hyperthermophilic heterotrophs were relatively high.
228 essure, which is significantly different for hyperthermophilic (IPPase) and mesophilic (HEWL) protein
229 sitions -40 to +1 of the gdh promoter of the hyperthermophilic marine archaea, Pyrococcus furiosus (P
230 s of this family have been identified in the hyperthermophilic marine archaeon Methanococcus jannasch
231 ha family (family B) DNA polymerase from the hyperthermophilic marine archaeon Thermococcus sp. 9 deg
232 of Thermofilum pendens, a deeply branching, hyperthermophilic member of the order Thermoproteales in
236 To our knowledge, Amt proteins are the first hyperthermophilic membrane transport proteins shown to b
237 d stability from the hot-start toward modern hyperthermophilic, mesophilic, and psychrophilic organis
238 thanocaldococcus jannaschii--a deeply rooted hyperthermophilic methanogen growing only on H2 plus CO2
240 first structures of archaeal G1PDH from the hyperthermophilic methanogen Methanocaldococcus jannasch
242 have been detected in a non-nitrogen-fixing hyperthermophilic methanogen, Methanocaldococcus jannasc
243 ata support our estimated H(2) threshold for hyperthermophilic methanogenesis at vents and highlight
244 pure culture H(2) threshold measurements for hyperthermophilic methanogenesis in low-temperature hydr
246 of genome sequence data from mesophilic and hyperthermophilic micro-organisms has revealed a strong
248 is a large oligomeric protein derived from a hyperthermophilic microorganism that is found near hydro
249 aled that, on a global scale, populations of hyperthermophilic microorganisms are isolated from one a
250 redicted to be particularly energy-rich, and hyperthermophilic microorganisms that broadly reflect su
252 The family 4 uracil-DNA glycosylase from the hyperthermophilic organism Archaeoglobus fulgidus (AFUDG
257 haracterisation of this enzyme isolated from hyperthermophilic organisms has led to its adoption as a
258 ete genomes for mesophilic, thermophilic and hyperthermophilic organisms have been sequenced, vastly
261 roteins have been characterized in extremely hyperthermophilic organisms, and most function as repres
262 ungsten, which substitutes for molybdenum in hyperthermophilic organisms, could also be ligated to mo
266 sing DNA composition bias in genomes of some hyperthermophilic organisms: simply screening for GC-ric
268 used thioredoxin (Trx) folds, belongs to the hyperthermophilic protein disulfide oxidoreductase famil
269 n enzymatically active, soluble variant of a hyperthermophilic protein that is normally insoluble whe
270 utions to the folding free energy of several hyperthermophilic proteins and their mesophilic homologs
271 aken together, our results suggest that many hyperthermophilic proteins enhance electrostatic interac
272 o 100 degrees C emphasizes the importance in hyperthermophilic proteins of the specific location of i
273 tabilizing forces required for extracellular hyperthermophilic proteins to tolerate high-temperature
274 of improving the low-temperature activity of hyperthermophilic proteins, likely by facilitating the i
275 in 1998, the method has been used to create hyperthermophilic proteins, to evolve novel folded domai
280 ys+1, in an intein precursor composed of the hyperthermophilic Pyrococcus abyssi PolII intein and ext
281 We report the solution NMR structures of the hyperthermophilic Pyrococcus abyssi PolII intein, which
282 idium pasteurianum (Topt = 37 degrees C) and hyperthermophilic Pyrococcus furiosus (Topt = 95 degrees
285 g agent, dextran 20, on the folded states of hyperthermophilic (S16Thermo) and mesophilic (S16Meso) h
286 coli and WrbA from Archaeoglobus fulgidus, a hyperthermophilic species from the Archaea domain, shows
288 d refined the structure of the mIPS from the hyperthermophilic sulfate reducer Archaeoglobus fulgidus
289 (diaphorase) activity was isolated from the hyperthermophilic sulfate-reducing anaerobe Archaeoglobu
291 inantly expressed components of SRP from the hyperthermophilic, sulfate-reducing archaeon Archaeoglob
292 onlytic temperate viruses were isolated from hyperthermophilic Sulfolobus hosts, and both viruses sha
293 loned and purified the RadA protein from the hyperthermophilic, sulphate-reducing archaeon Archaeoglo
294 ior previously observed for thermophilic and hyperthermophilic superoxide dismutases but over a lower
295 um, a facultatively aerobic nitrate-reducing hyperthermophilic (T(opt) = 100 degrees C) crenarchaeon.
297 , it has been suggested that thermophilic or hyperthermophilic (Tm) enzymes have lower catalytic powe
299 scuous natural human IgG-binding domain, the hyperthermophilic variant of protein G (HTB1), into a hi
300 , single-point core mutants of a 57-residue, hyperthermophilic variant of the B1 domain of protein G
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