ライフサイエンスコーパス: iberiotoxin

1 +/-1 vs. 10+/-1 and 19+/-1% before and after iberiotoxin).

2 ium chloride, 70 nM charybdotoxin, or 100 nM iberiotoxin).

3 ilation (36+/-1 vs. 14+/-2% before and after iberiotoxin).

4 ontrol and moderate hypoxia before and after iberiotoxin).

5 nd 40-min hypoxic dilations before and after iberiotoxin).

6 d intact muscle strips, an effect blocked by iberiotoxin.

7 are unchanged by the Kca channel antagonist iberiotoxin.

8 ries and by 158 +/- 12 nM in the presence of iberiotoxin.

9 rons isolated from embryos were unchanged by iberiotoxin.

10 and U50488H all were similarly attenuated by iberiotoxin.

11 cAMP analogue, 8-bromo cAMP, was blunted by iberiotoxin.

12 ation was also inhibited by BaCl2 but not by iberiotoxin.

13 ntracellular BaCl2 or by 10 nM extracellular iberiotoxin.

14 loxyphenyl)hexanoic acid, sulfaphenazole, or iberiotoxin.

15 onents sensitive to apamin, clotrimazole and iberiotoxin.

16 calcium-activated potassium channel blocker iberiotoxin.

17 es when exposed to the MaxiK channel blocker iberiotoxin.

18 -type hSlo channels were sensitive to 100 nM iberiotoxin.

19 onductance Ca(2+)-dependent K+ channels with iberiotoxin (0.1 microM) and apamin (1 microM), respecti

20 e pharmacological block of K(Ca) channels by iberiotoxin (0.1 to 100 nmol/L) dose-dependently constri

21 ive BKCa channel blocker paxilline (100 nm), iberiotoxin (10 mum), Ca(2+) free solutions and divalent

22 ed by the Ca2+-dependent K+ channel blockers iberiotoxin (10 nM) and charybdotoxin (10 nM).

23 attenuated by the Kca+2 channel antagonist, iberiotoxin (10(-7) M) (7 +/- 1, 11 +/- 1 and 16 +/- 1 v

24 Iberiotoxin (10(-7) M) attenuated hypoxic dilation but h

25 Iberiotoxin (100 nM) and apamin (50 nM), toxins known to

26 8-pCPT-AM-activated STOCs were sensitive to iberiotoxin (100 nM) and to ryanodine (30 muM).

27 conductance K(+) (BK(Ca)) channel opening as iberiotoxin (100 nM) significantly reduced the ability o

28 channels (TEA (1 mM), paxilline (10 muM) and iberiotoxin (100 nM)).

29 Iberiotoxin (100 nM), a blocker of KCa channels, under t

30 ensitive Ca2+ release (RyR) channel blocker; iberiotoxin (100 nM), a large-conductance Ca2+-activated

31 s also 56% blocked by the BK channel blocker iberiotoxin (100 nM).

32 but, unlike BK channels, are insensitive to iberiotoxin (100 nmol/L).

33 -sensitive potassium channel inhibitor), and iberiotoxin (100 nmol/L, calcium-activated potassium cha

34 two Ca(2+)-activated K(+) channel blockers (iberiotoxin, 100 nm and apamin, 1 mum).

35 and the Ca2+-dependent K+ channel inhibitor iberiotoxin (20 nM) failed to depolarize these cells fur

36 ckers with a combination of apamin 1 microM, iberiotoxin 200 nM, and clotrimazole 500 nM; 3) blocking

37 rs tetraethylammonium ions (TEA(+); 1 mM) or iberiotoxin (200 nM).

38 uring local perifusion with KCa antagonists, iberiotoxin (5 microm) had no effect, but charybdotoxin

39 were minimally affected by apamin (100 nM), iberiotoxin (50 nM), or ketoconazole (10 microM).

40 potassium channel (BK(Ca) channel) inhibitor iberiotoxin (50 nM).

41 0(-6) M) was attenuated by glibenclamide and iberiotoxin (8+/-1 and 17+/-1 vs. 4+/-1 and 9+/-1% befor

42 The dilation was largely abolished by iberiotoxin, a BK(Ca) channel blocker.

43 gly, by charybdotoxin (ChTX; 100 nM) but not iberiotoxin, a charybdotoxin analogue, which blocks the

44 Iberiotoxin, a KCa channel blocker, reduced H(2)S-induce

45 Iberiotoxin, a MaxiK blocker, antagonizes the relaxation

46 contractions had a decreased sensitivity to iberiotoxin, a selective BK channel inhibitor, in DSM st

47 umor capillaries, and could be attenuated by iberiotoxin, a selective inhibitor for calcium-dependent

48 ellular K+ with Cs+ or by the application of iberiotoxin, a selective inhibitor of large-conductance,

49 Per1::GFP neurons under voltage clamp using iberiotoxin, a specific BK channel blocker.

50 Both were blocked by iberiotoxin, a specific blocker of large-conductance K(C

51 Coadministration of L-NAME and iberiotoxin almost abolished the vasodilation induced by

52 Because iberiotoxin also inhibited abn-cbd-induced relaxation of

53 V that was abolished by KT5823, or by 100 nM iberiotoxin (an inhibitor of BKCa channels).

54 Iberiotoxin, an inhibitor of BK channels, increased the

55 In contrast, iberiotoxin and apamin did not block the effects of apic

56 lammonium; similar results were evident with iberiotoxin and charybdotoxin block.

57 In oocytes, iberiotoxin and charybdotoxin, peptidyl scorpion toxins,

58 and inhibited by the BK channel inhibitors, iberiotoxin and charybdotoxin.

59 Specific inhibitors of these channels, iberiotoxin and paxilline, blocked oxidase-induced 86Rb+

60 In contrast, the BK(Ca) channel blockers iberiotoxin and paxilline, the phosphoinositide 3-kinase

61 ic K(Ca) current of E13 LMNs is inhibited by iberiotoxin and resistant to apamin.

62 Iberiotoxin and ryanodine, a ryanodine receptor channel

63 by the BK Ca2+-activated K+ channel blocker iberiotoxin and unaffected by apamin, indicating selecti

64 r pressures, and reduced vasoconstriction to iberiotoxin and vasodilation to NS1619, BK channel inhib

65 ce calcium-activated potassium channels with iberiotoxin, and is abolished by blocking small conducta

66 t human KSper is inhibited by charybdotoxin, iberiotoxin, and paxilline, while mouse KSper is insensi

67 of endothelial integrity, prevented by 55 nM iberiotoxin, and unmodified by 0.8 mM 4-aminopyridine, i

68 were mediated by inhibition of Ca-activated iberiotoxin- and apamin-sensitive K channels, but only i

69 ed by external TEA but not by charybdotoxin, iberiotoxin, apamin, or 4-aminopyridine.

70 um as a LRET donor and a fluorophore-labeled iberiotoxin as the LRET acceptor for measurements of dis

71 BKCa channel blockers paxilline and iberiotoxin, as well as Ca(2+) free solutions and divale

72 rosoyasaponin-I-induced activation, and (iv) iberiotoxin blockade.

73 Iberiotoxin broadened action potentials (APs), indicatin

74 dependent and sensitive to charybdotoxin and iberiotoxin but not to apamin, suggesting that they were

75 educed by 1 mM 4-aminopyridine and/or 100 nM iberiotoxin but unaffected by 10 nM dendrotoxin-K.

76 Iberiotoxin, but not apamin, inhibited STOC activity in

77 served in the presence of the BK antagonist, iberiotoxin, but persisted in the presence of KATP and S

78 Iberiotoxin did not affect the resting potential but inh

79 Inhibition of BK channels with iberiotoxin did not alter the fEPSPs in inflamed tissue,

80 Analysis of iberiotoxin difference currents also demonstrated that B

81 Iberiotoxin enhanced myogenic tone in both groups but mo

82 Iberiotoxin extended the duration of ventral root bursts

83 Coadministration of L-NNA with iberiotoxin further decremented hypoxic pial dilation an

84 by >= 0.1 mM barium (Ba2+) and unaffected by iberiotoxin, glibenclamide, apamin, 3,4-DAP and ouabain.

85 n of calcium-activated potassium channels by iberiotoxin had no effect on this dilation.

86 Iberiotoxin had no further effect on whole-cell K+ curre

87 The K(ca) channel antagonist iberiotoxin had no influence on pial dilation during 5 m

88 Iberiotoxin (IbTX or alpha-KTx 1.3), a selective, high-a

89 Substituting iberiotoxin (IbTX) for CbTX greatly diminished inhibitio

90 mino acids comprising the alpha/beta turn in iberiotoxin (IbTX) replaced the corresponding seven amin

91 that generates NO, were reduced by > 50% by iberiotoxin (IBTX), an inhibitor of Ca(2+)-dependent K+

92 The pore blockers charybdotoxin (CTx) and iberiotoxin (IbTx), at nanomolar concentrations, have be

93 rostone isopropyl and M1 activated sustained iberiotoxin (IbTX)-sensitive, AL-8810 (FP receptor antag

94 ted in a 30-50% increase in the amplitude of iberiotoxin (IBTX)-sensitive, whole-cell K(+) current.

95 ve Ca(2+)-dependent K+ channel (KCa) blocker iberiotoxin (IbTx, 100 nm) reduced IK in glomus cells fr

96 presence and absence of the BKCa inhibitor, iberiotoxin (IBTX; 0.1 microM).

97 hetized rats: (I) KCa channel-inhibited (via iberiotoxin); (II) KATP channel-inhibited (via glibencla

98 re inhibited by 4-AP, TEA, charybdotoxin and iberiotoxin implicating functional K(v) and BK(Ca) chann

99 Slo-/- mice and by blocking BK channels with iberiotoxin in the Slo+/+ strips.

100 Iberiotoxin increased the amplitude and frequency of pha

101 Blockade of BK channels (using iberiotoxin) increased action potential amplitude and en

102 > old coronary arteries (explained by larger iberiotoxin-induced contraction and decreased dynamic ra

103 tivated a K+-selective, apamin-sensitive and iberiotoxin-insensitive current, detected as a tail curr

104 Our findings suggest that apamin- and iberiotoxin-insensitive K(Ca) channels are subject to di

105 Glibenclamide and iberiotoxin, K(ATP) and K(ca) channel antagonists, atten

106 387 +/- 34 nM), but is resistant to apamin, iberiotoxin, kaliotoxin, scyllatoxin (Kd > 1 microM), an

107 Glibenclamide and iberiotoxin, KATP and Kca channel antagonists, attenuate

108 % of the outward current was also blocked by iberiotoxin (Kd = 36 nM).

109 response to 8-pCPT-AM that was sensitive to iberiotoxin (n = 5).

110 harybdotoxin (NPo, 37% of control), or 10 nM iberiotoxin (NPo, 5% of control), whereas neurohypophysi

111 n contractions were similar to the effect of iberiotoxin on control mice.

112 Iberiotoxin or 1 mM tetraethylammonium (TEA+) constricte

113 ng outward currents, which were abolished by iberiotoxin or by chelation of intracellular calcium.

114 t with Ca2(+)-activated K+ channel blockers, iberiotoxin or charybdotoxin, did not prevent potentiati

115 in VSMC that was prevented by coinfusion of iberiotoxin or of AG-1478.

116 Application of iberiotoxin or paxilline, blockers of Maxi-K channels, m

117 nnel antagonist tetraethylammonium chloride, iberiotoxin, or 4-aminopyridine.

118 vessels elicited K(+) channel opening in an iberiotoxin- or PEG-CAT-sensitive fashion in cell-attach

119 When these channels were blocked by iberiotoxin outward current was significantly reduced in

120 H arteries, and this dilation was blocked by iberiotoxin, paxilline, and KCl preconstriction but not

121 Tetraethylammonium and iberiotoxin, preferential KCa-channel inhibitors, attenu

122 e (5-HT) contractile efficacy was reduced by iberiotoxin pretreatment in young > old coronary arterie

123 )-activated K(+) (BK(Ca)) channel inhibitor, iberiotoxin, produced identical inhibition of resveratro

124 a(2+)-activated K(+) channel (charybdotoxin, iberiotoxin, quinine, and Ba(2+)) nor inhibitors of the

125 tetraethylammonium-sensitive (IC50 = 9 mM), iberiotoxin-resistant, delayed rectifier K+ current and

126 -8), 10(-6) M in the absence and presence of iberiotoxin, respectively).

127 sion of gBK in Xenopus oocytes gives rise to iberiotoxin-sensitive (IbTX) currents, with an IC(50) fo

128 (Ca)) subtypes are decreased by axotomy, but iberiotoxin-sensitive and clotrimazole-sensitive current

129 lly, inhibition of CYP-epoxygenase abolished iberiotoxin-sensitive and flow-stimulated but not basal

130 of outward K(+) current detected a prominent iberiotoxin-sensitive BK(Ca) current in SMCs that was ab

131 annels, including apamin-sensitive channels, iberiotoxin-sensitive channels, and channels that are in

132 A or PP2, indicating that c-Src inhibits the Iberiotoxin-sensitive component, likely MaxiK channels.

133 all-sized control DRG neurons also expressed iberiotoxin-sensitive currents, which are reduced in bot

134 iments revealed a 4.7-fold higher density of iberiotoxin-sensitive K(Ca) channel current at physiolog

135 nctional studies demonstrated MYOCD-induced, iberiotoxin-sensitive potassium currents in porcine coro

136 Iberiotoxin-sensitive voltage- and [Ca2+]-activated K+ c

137 patch clamp recordings could not detect BK (iberiotoxin-sensitive) currents in cultured BAECs under

138 mV) cells, H(2)S increased the frequency of iberiotoxin-sensitive, Ca(2)(+) spark-induced transient

139 zation (7.9 +/- 3.9 mV) due to activation of iberiotoxin-sensitive, maxi-K(Ca) channels.

140 Inhibition by tetraethylammonium and iberiotoxin suggested that these currents represented ac

141 s (riluzole) and several potassium channels (iberiotoxin, TEA, 4-amino-pyridine), but blockers of cal

142 tes were more sensitive to the peptide toxin iberiotoxin than were hSlo + hSlobeta currents, and the

143 n/function in older myocytes, the ability of iberiotoxin to contract coronary rings was reduced appro

144 y effective blockers of BK current, although iberiotoxin was significantly more potent than charybdot

145 tion of outward current by MCD pretreatment, iberiotoxin was unable to produce any additional decreas

146 ge conductance channel was sensitive to TEA, iberiotoxin, was activated in excised inside-out patches

147 The effects of ryanodine and iberiotoxin were not additive and were blocked by inhibi

148 tifying K+ currents, reversibly inhibited by iberiotoxin, were demonstrated in neutrophils and eosino

149 bout 53 % of IKCa was selectively blocked by iberiotoxin which had no effect on the delayed rectifier