ライフサイエンスコーパス: imino proton

1 One of these involves a rapidly exchanging G imino proton.

2 lacking due to the rapid exchange of the U23 imino proton.

3 Imino proton and phosphorus NMR spectra suggest that som

4 intensities of the Watson-Crick base-paired imino protons and a reduction by 20 degrees C in the upp

5 Upfield chemical shifts of duplex imino protons and the disruption of the NOE base-sugar c

6 Psi has two hydrogen donors (N1 and N3 imino protons) and two hydrogen bond acceptors because t

7 rbance melting curves, NMR titrations of the imino protons, and analysis of NMR spectra in the presen

8 The exchange times for Watson-Crick imino protons are approximately 1 h for both triplexes.

9 The exchange kinetics of the imino protons are measured from selective longitudinal r

10 Using NOESY, the imino protons as well as the cytosine amino protons of a

11 All imino protons associated with the iG:C, G:iC, and A:T (e

12 the selectively enhanced exchange rates for imino protons associated with these base pairs.

13 ls due to protons on carbon 2 of adenine and imino protons at the central five A-T pairs of the 11 ba

14 sequences, the solvent exchange rates of the imino protons at the junction of the helical stems in th

15 , as well as the upfield shifts observed for imino protons at this step, serve to define the central

16 o duplex as evident from the presence of the imino proton (at 13.70 ppm) of an A:T base pair.

17 d the U8-H6 and U8-H5, thus orienting the U8 imino proton away from U5.

18 Increasing the pK(a) value of the imino proton by reduction of its 5,6-double bond results

19 d of Mg2+to P5 were determined by monitoring imino proton chemical shifts during titration of the RNA

20 the dramatic change that IHF imposes on the imino proton chemical shifts is indicative of a severe d

21 Analysis of imino proton chemical shifts show that structural pertur

22 rbations at the binding-site as reflected in imino proton complexation shifts and specific altered su

23 ructure models that are indistinguishable in imino proton connectivities.

24 e calculated values of the exchange rates of imino protons, especially those obtained from measuremen

25 r in the two structures are characterized by imino proton exchange and nuclear magnetic resonance spe

26 The kinetics of imino proton exchange as a function of basic pH or added

27 opening rates in RNA are consistent with the imino proton exchange experiments for AU base pairs, alt

28 shows that nuclear magnetic resonance-based imino proton exchange is a good probe for detection of w

29 Imino proton exchange measurements also yielded the diss

30 -terminal bases were determined by measuring imino proton exchange rates as a function of exchange ca

31 Measurements of ligand amide and DNA imino proton exchange rates indicate very slow dissociat

32 xternal catalysis exchange mechanisms on the imino proton exchange rates is analyzed.

33 characterizing the temperature dependence of imino proton exchange rates of individual basepairs.

34 perature dependence of the UV absorption and imino proton exchange rates provides insight into the st

35 base pair opening parameters estimated from imino proton exchange rates suggest that the stability o

36 ches derived from 1H NMR measurements of the imino proton exchange rates.

37 spectroscopy and by measuring base-catalyzed imino proton exchange rates.

38 appear to close more slowly than the rate of imino proton exchange with bulk water, since their excha

39 to characterize the effects of a catalyst of imino proton exchange, namely, ammonia upon the structur

40 This is surprising, given that imino-proton exchange rates show that basepairs in a DNA

41 ntration, the exchange times of the mismatch imino protons extrapolate to much shorter lifetimes than

42 TAR allowed observation of the C23 amino and imino protons for the first time, providing direct evide

43 pening at X(6).T(17) and protected the T(17) imino proton from solvent exchange.

44 The imino protons from the different mismatched, as well as

45 lly greater than that of the corresponding T imino proton; however, this difference is not attributed

46 The exchange characteristics of the imino protons identify the existence of four additional

47 We have measured the exchange rates of imino protons in each triad of the three triplexes using

48 The results indicate that the exchange of imino protons in protonated cytosines is most likely lim

49 as the solvent accessibility of the unpaired imino protons in the complex.

50 nge of individual Watson-Crick and Hoogsteen imino protons in the DNA triple helix were measured in t

51 parative studies of hydrogen exchange of the imino protons in the free RNA aptamer and the AMP-RNA ap

52 ic reduction in exchange is observed for the imino protons in the IHF bound DNA.

53 aracteristics of the extremely well resolved imino protons in the NMR spectrum of the complex.

54 e pair opening were measured for most of the imino protons in the P1 duplex using the base catalysts

55 exchange cross peaks for several base-paired imino protons in the RNA yielded an apparent k(on) of 60

56 that Mg2+ lowers the exchange rates of most imino protons in the structure by stabilizing the corres

57 The exchange rates of imino protons in this triple helix have been measured by

58 the triplex structure, the exchange rates of imino protons in Watson-Crick base pairs are up to 5000-

59 The downfield shift of the 3'-G imino protons indicates the complex makes hydrogen bond

60 n NMR spectrum shows resonances expected for imino protons involved in guanine quartet base-pairing.

61 nternal Psi-A, Psi-G and Psi-U pairs, the N3 imino proton is hydrogen bonded to the opposite strand n

62 NMR studies of imino proton lifetime, solvent accessibility, and NOE co

63 to the opposite strand nucleotide and the N1 imino proton may also be hydrogen bonded.

64 One-dimensional imino proton NMR and circular dichroism spectra of the m

65 Imino proton NMR data provided evidence that elements of

66 Ribonuclease mapping of T4-32 and imino proton NMR experiments of T4-35 show that both seq

67 Imino proton NMR experiments provide evidence for possib

68 Imino proton NMR indicates that this is partly because t

69 For selected sequences, imino proton NMR provides evidence that the desired dupl

70 Imino proton NMR spectra were also measured for two syst

71 UV and imino proton NMR spectral observations indicated pronoun

72 The temperature-dependent imino proton NMR spectrum of oxoG modified DNA confirms

73 lts from UV experiments were corroborated by imino proton NMR studies that show proton exchange rates

74 studied by optical melting, one-dimensional imino proton NMR, and one-dimensional phosphorus NMR.

75 oresis, equilibrium ultracentrifugation, and imino proton NMR, we are able to show that these modific

76 nternal loops were studied by UV melting and imino proton NMR.

77 m mismatches, were studied by UV melting and imino proton NMR.

78 e versus temperature melting experiments and imino proton nuclear magnetic resonance (NMR).

79 The observed imino proton nuclear magnetic resonance resonances and F

80 nd carbonyl oxygen of G, another between the imino proton of G and carbonyl oxygen O2 of U.

81 largest chemical shift change occurred at an imino proton of one of the G.A base-pairs, no nuclear Ov

82 ed in 95% H(2)O, 5% D(2)O indicated that the imino proton of the base opposite N14, G5, or T5, formed

83 The line width of the imino proton of the ClU residue is substantially greater

84 eased level of chemical exchange for the ClU imino proton of the ClU-A base pair, the ClU residue is

85 The imino proton of the lesion-containing base pair resonate

86 ective changes to the chemical shifts of the imino protons of a GCGA tetraloop in the 75mer, that is

87 imensional NOE data show no NOEs between the imino protons of U5 and U8, but NOEs are observed betwee

88 Functional group substitution shows that the imino proton on the N1 is critical, suggesting a possibl

89 from the dependence of the exchange rates of imino protons on ammonia concentration.

90 The imino proton region of 1-D NMR spectra shows that G.G an

91 One-dimensional (1)H NMR spectra of the imino proton region of I in the presence of this series

92 Observation of an imino proton resonance at 15.6 p.p.m. provides evidence

93 he 1.13 ppm upfield shift of the thioguanine imino proton resonance, and the large increase in the ex

94 Analysis of imino proton resonances and NOEs indicates additional en

95 ding affinity was measured by monitoring RNA imino proton resonances for some of the compounds that s

96 by pronounced upfield shifts of the G-tetrad imino proton resonances in the NMR, which is similar to

97 Imino proton resonances indicate the hydrogen bonding pa

98 addition, the temperature dependence of the imino proton resonances is also consistent with the diff

99 clear NMR experiments aided in assigning the imino proton resonances of the DNA alone and in complex

100 The assignment of the imino proton resonances with characteristic chemical shi

101 The temperature dependence of the imino proton resonances, as well as UV absorption data,

102 ive imaging agents, since their exchangeable imino protons resonate at 5-6 ppm from the water proton

103 rees C in the upper temperature at which the imino proton signals are detectable, consistent with des

104 The N3-nitrogen and imino proton signals of an (15)N-labeled thymine dimer-c

105 nding interface was obtained from changes in imino proton signals of uniformly 15N-labeled Tar with i

106 Thermal denaturation and imino proton solvent exchange experiments reveal that th

107 and the exchange rates of the corresponding imino protons, suggest that these two basepairs open in

108 The downfield shift of the G8 imino proton supports the conclusion that the pendant gu

109 Chemical shift changes of the imino protons upon titration of the RNA hairpin with Mg(

110 d from measurements of the exchange rates of imino protons using nuclear magnetic resonance spectrosc

111 However, the expected U23 imino proton was not observed, preventing unambiguous id

112 The resonance arising from the T(17) imino proton was not observed.

113 rates of exchange for the slowest exchanging imino protons were approximately 20 times faster in unmo

114 NOE connectivities between imino protons were disrupted at T16 and T17.

115 ar set of slowly exchanging (t(1/2) > 3 min) imino protons were observed in both tRNAs, but the rates

116 OESY WATERGATE spectra show an NOE between U imino protons, which suggests that U4 and U9 form a hydr

117 ease in the exchange rate of the thioguanine imino proton with water.

118 The rates of exchange of imino protons with solvent in the two structures have be

119 erized by measuring the rates of exchange of imino protons with solvent protons as a function of the

120 The exchange of imino protons with solvent protons was measured for each

121 The rates of exchange of imino protons with solvent protons were measured by NMR

122 complex with IHF, the exchange rates of the imino protons with the solvent have been measured for H1

123 pon measurements of the rates of exchange of imino protons with water protons at high concentrations