ライフサイエンスコーパス: immobilized enzyme

1 lide hydrochloride (BApNA) were made for the immobilized enzyme.

2 cancer therapy and chemoprevention), by the immobilized enzyme.

3 % of the activity obtained in buffer for the immobilized enzyme.

4 spectra before and after incubation with the immobilized enzyme.

5 higher than the values for systems based on immobilized enzymes.

6 l analytical microsystems containing surface-immobilized enzymes.

7 er physically adsorbed enzymes or covalently immobilized enzymes according to the usual immobilizatio

8 hydration and exposure to denaturants on the immobilized enzyme activity are presented.

9 ans for pesticide detoxification in place of immobilized enzymes, affording a reduced diffusional bar

10 The immobilized enzyme also exhibited good operational stabi

11 trices, non-toxicity, increased stability of immobilized enzyme and effective hydrolysis of RFOs incr

12 to facilitate electron transfer between the immobilized enzymes and electrode surfaces, better confo

13 tre-sized beads, tunable activity of surface-immobilized enzymes and reversible encapsulation of adhe

14 s that chitosan-grafted hydrogels containing immobilized enzymes are useful for the production of lac

15 ZnONR imparted significant stability to the immobilized enzyme as against physisorbed enzyme.

16 ingly wide range of devices that use surface-immobilized enzymes as integral components with improved

17 Most often, the immobilized enzyme based quantification is an attractive

18 to fluorescent species, and (ii) a novel non-immobilized enzyme-based fluorescence assay strategy, fe

19 may also have utility for the production of immobilized enzyme bioreactors.

20 her, several schemes on porous silicon-based immobilized enzyme biosensors for the detection of chrom

21 etects binding of 3H-labeled DNA to the bead-immobilized enzyme by scintillation counting.

22 atively, uncertainties in the activity of an immobilized enzyme can be accounted for using standard a

23 In addition, the immobilized enzyme can be cleaned easily, enabling the m

24 It is further shown that the immobilized enzyme can be recycled and reused for contin

25 The immobilized enzyme can be reused for multiple cycles of

26 The immobilized enzyme can be used effectively for the hydro

27 ing previously published mutations, and this immobilized enzyme can be used for the modification of c

28 l that chitosan-grafted hydrogels containing immobilized enzymes can be reused several times without

29 bes an exciting technology that makes use of immobilized enzyme-catalysts for condensation polyester

30 locity, V'(max), is linearly proportional to immobilized enzyme concentration, but the magnitude is a

31 This new vehicle for immobilized enzymes, created entirely from biomolecules,

32 ization method, geometry, enzyme loading) of immobilized-enzyme devices.

33 ensure the electrocatalytic activity of the immobilized enzyme, didodecyldimethylammonium bromide (D

34 Immobilized enzyme digestion resulted in more partial cl

35 significant decrease in the activity of the immobilized enzyme during reutilization studies.

36 d T4 proteins was passed through a column of immobilized enzyme (either dTMP synthase or dCMP deamina

37 iently integrated in the process, and use of immobilized enzymes entails an economical advantage.

38 We have termed this method "immobilized enzyme ESR," abbreviated IE-ESR.

39 (top) membrane contains an electrostatically immobilized enzyme for the catalytic production of one o

40 was applied to determine the kinetics of the immobilized enzymes for CO2 absorption.

41 solution is approximately 4x higher than the immobilized enzyme, for which we measured an apparent K'

42 Flowing substrate solution over the immobilized enzyme generates in situ, transient radicals

43 The immobilized enzyme had higher thermal stability than the

44 characterize the interfacial orientation of immobilized enzymes has been developed using a combinati

45 ications, relatively few processes employing immobilized enzymes have been successfully commercialize

46 The activity of the immobilized enzyme in air approaches 25% of the activity

47 The immobilized enzymes in anode and cathode are very stable

48 n of Michaelis-Menten kinetic parameters for immobilized enzymes in microfluidic reactors are address

49 review the focus is set on the evaluation of immobilized enzymes in respect to mass transport limitat

50 Since the large-scale application of immobilized enzymes in the 1960s, substantial research e

51 hed and emerging industrial applications for immobilized enzymes, including high-fructose corn syrup

52 The immobilized enzyme/inhibitor mixture is centrifuged, and

53 l modification can be detected as long as an immobilized enzyme is available that reacts with the mod

54 ith potential substrates, albeit only if the immobilized enzyme is electroactive.

55 The catalytic activity of the immobilized enzymes is monitored in real time by reflect

56 product, coupled with the efficiency of the immobilized enzyme, is the determining factor for the se

57 aelis constant KM of (122 +/- 4) muM for the immobilized enzyme layer.

58 odel protein to study how the orientation of immobilized enzyme molecules on surfaces affects their c

59 strate high enzymatic activity levels of the immobilized enzymes (more than 80%), while maintaining t

60 Here, the use of the immobilized enzyme Novozym 435 to efficiently catalyze t

61 e library is subsequently incubated with the immobilized enzyme of interest under the correct conditi

62 application of soluble enzymes in industry, immobilized enzymes often offer advantages in view of st

63 requency (371.1 Hz) of norepinephrine to the immobilized enzyme on a gold disk electrode.

64 The amount of immobilized enzyme on Fe3O4@SiO2@p(GMA) and Fe3O4@SiO2@p

65 to systematically investigate the covalently immobilized enzyme penicillinase under different experim

66 The immobilized enzyme possessed a better heat-resistance th

67 The immobilized enzyme presented higher thermal stability th

68 tes (WPC) were hydrolyzed with both free and immobilized enzyme, reaching a similar degree of hydroly

69 ed trypsin digestion using a continuous flow immobilized enzyme reactor (cf-IMER).

70 The first step uses an immobilized enzyme reactor (IMER) column interfaced to a

71 igestion efficiency of the, thiol-ene based, immobilized enzyme reactor (IMER) is compared to that of

72 ncreasing trypsin concentration by use of an immobilized enzyme reactor (IMER).

73 Applications ranging from immobilized enzyme reactors to fast media for the separa

74 The immobilized enzyme retained catalytic property even afte

75 The immobilized enzyme retained its bioactivity, exhibited a

76 The immobilized enzymes retained 56-88% of their original ac

77 The immobilized enzymes retained up to 75% of residual activ

78 ard electropolymerized films of PPB with the immobilized enzyme retaining virtually all of its enzyma

79 The immobilized enzyme showed 70% activity after 30days of s

80 The immobilized enzyme showed higher Km 2.08mg/ml than the f

81 After ten successive runs, the immobilized enzyme still retained approximately 60% acti

82 was specifically purified by binding to the immobilized enzyme substrate and the cDNA was cloned in

83 mploying biocompatible material gallic acid, immobilized enzyme system was synthesized along with imp

84 Immobilized enzyme systems are important in a broad rang

85 e with similar kinetic restrictions as other immobilized enzyme systems.

86 Inhibitor libraries are screened by using immobilized enzyme technologies and electrospray ionizat

87 he first example of a biosensor that uses an immobilized enzyme that possesses the tryptophan tryptop

88 Here we show that surface-immobilized enzymes that are independent of adenosine tr

89 This enzymatic fuel cell is based on non-immobilized enzymes that exhibit a maximum power output

90 Immobilized enzymes that most likely undergo single-enzy

91 Immobilized enzymes typically have greater thermal and o

92 Measuring the catalytic activity of immobilized enzymes underpins development of biosensing,

93 onstant (K(m)) of 0.12 mM, indicate that the immobilized enzyme (uricase) has enhanced affinity towar

94 The Vmax of immobilized enzyme was about 2.92+/-.02mg/ml/min and tha

95 The turnover number of the immobilized enzyme was estimated to be 65s(-1) which is

96 The immobilized enzyme was evaluated and found to have an ap

97 The immobilized enzyme was stable and not inhibited by other

98 A newly developed ESR technique that uses immobilized enzyme was used to perform the ESR experimen

99 ity and reusability for starch hydrolysis of immobilized enzyme were obtained.

100 These hydrogels containing immobilized enzymes were employed to simulate the produc

101 In addition, the immobilized enzymes were more stable than the free enzym

102 best reaction yields were obtained when the immobilized enzymes were treated with LPG.

103 Immobilized enzymes will be dispersed on the support sur

104 Electrochemical characterization of the immobilized enzyme with GO and DDAB on glassy carbon ele