ライフサイエンスコーパス: immobilized protein

1 l as charged amino acids associated with the immobilized protein.

2 developed using magnetic beads containing an immobilized protein.

3 robing the homogeneity of the populations of immobilized protein.

4 arfarin is small compared to the size of the immobilized protein.

5 he visible range around 425nm, caused by the immobilized proteins.

6 robust single-molecule detection of surface-immobilized proteins.

7 flowed through the capillary to bind to the immobilized proteins.

8 gel matrix; and (v) in-gel immunoprobing of immobilized proteins.

9 can influence the binding activities of the immobilized proteins.

10 anic complexes shuttle electrons between the immobilized proteins.

11 nstant (Ka) for human IgG binding to surface-immobilized protein A is determined to be (3.0 +/- 0.5)

12 ques measuring biotinylated gC1qR binding to immobilized protein A revealed specific binding that was

13 o that in NHS preabsorbed by the yeast or by immobilized protein A/G to remove serum immunoglobulin.

14 In addition, immobilized protein activity levels were high, typically

15 of the formation of a bound complex between immobilized protein and immobilized ligand as a function

16 ays are based on the interaction between the immobilized protein and the sampled analyte directly on

17 The activity of the immobilized protein and the surface density were determi

18 ecifically designed for staining NC membrane-immobilized proteins and is faster and more sensitive th

19 on of resolved proteins, antibody probing of immobilized proteins, and all interim wash steps.

20 s used for investigating the conformation of immobilized proteins, and then examines how protein load

21 Because no stripping is involved, immobilized proteins are not lost from the membrane, thu

22 This research describes how to prepare immobilized protein arrays and how to assay the binding

23 RNA pull-down and immobilized protein assays showed that hrp48 protein bin

24 scaffold for subsequent antibody probing of immobilized protein bands.

25 sed on analysis of protein conformation, the immobilized proteins bind with partner antibody after SD

26 Moreover, using an immobilized protein-binding assay, we found that phospho

27 ndent fashion, Kd (app) = 29 nM and bound to immobilized protein C in a Ca2+-dependent fashion.

28 of a complex between immobilized ligand and immobilized protein close to that for the formation of c

29 iles has been developed, based on the use of immobilized protein columns and HPLC.

30 st the epsilon subunit was passed over these immobilized protein columns, and the purified antibodies

31 residual activity of 40% for the covalently immobilized protein compared to 20% of residual activity

32 igated the binding of fluid-phase CRP to six immobilized proteins: complement factor H, oxidized low-

33 double resonance (ELDOR) spectroscopy on an immobilized protein containing RX all reveal a highly co

34 In this experiment, the immobilized protein content and LDH activity on each mod

35 t 3 logs was observed for protein stains and immobilized protein content, with a lower limit of detec

36 obilized proteins, it was necessary that the immobilized proteins, except factor H, were also exposed

37 of high-capacity silica supports containing immobilized protein G.

38 C4BP bound to immobilized protein H or AP1 bacteria retained its cofac

39 Arrays of immobilized proteins have been developed for the discove

40 dividual protein kinases on a complex mix of immobilized proteins, (ii). the fractionation of the pho

41 ely for isolating aptamers against a surface-immobilized protein (immunoglobulin E) and a solution-ph

42 d thioredoxin showed the presentation of the immobilized protein in a sterically accessible orientati

43 americ H2O2-treated CRP bound to a number of immobilized proteins including oxidized LDL, IgG, amyloi

44 d support, and because the orientation of an immobilized protein is important for its function.

45 Understanding the properties of immobilized proteins is critical to the optimal design o

46 ase interactions between small molecules and immobilized proteins is of intense interest, especially

47 fficient binding of acidic pH-treated CRP to immobilized proteins, it was necessary that the immobili

48 s developed, in which both the deposition of immobilized protein layers and the electrophoretic deliv

49 e quantify relates linearly to the number of immobilized protein molecules (R(2) = 0.98), and our pre

50 Immobilized protein molecules on the surface of an alumi

51 us, our procedure allows for single, surface-immobilized protein molecules to be detected with high s

52 rd compact disc (CD) as a biochip containing immobilized protein molecules.

53 of multistep reaction procedures leading to immobilized proteins on solid surfaces.

54 ction screening against a dilution series of immobilized proteins on the microarray enabling the obse

55 teins to acidic pH alter the conformation of immobilized proteins, our findings suggest that conforma

56 proach resulted in random orientation of the immobilized proteins over the surface.

57 on of low molecular weight ligand binding to immobilized protein receptors.

58 The immobilized proteins remain bioactive, as evidenced by e

59 gions and conditions studied for the surface immobilized proteins showed restricted motion, with indi

60 l inhibitory factor (NIF), C3bi, and certain immobilized protein substrates, represented by denatured

61 superficially inserted state; and 3), fully immobilized proteins, suggesting a fully inserted state.

62 ing between small molecules and a variety of immobilized protein targets, pairs that have binding aff

63 " packed with the same resin but without the immobilized protein to evaluate the dead volume, but thi

64 roteins on microtiter plates and exposure of immobilized proteins to acidic pH alter the conformation

65 Subsequent incubation of the immobilized protein using a fluorescently labeled or PEG

66 on of substantial conformational changes for immobilized proteins using SPR has been reported.

67 scence from single molecules attached to the immobilized proteins was detected with a high signal/noi

68 rase and lysozyme--the highest quantities of immobilized proteins were obtained when using a low ioni

69 and binding constants for the association of immobilized protein with free and immobilized ligand.