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1 n 70 (HSP70) IgG was isolated from plasma by immunoaffinity.
2 GFR protein (CD271, p75NTR) were isolated by immunoaffinity adsorption, and cultured as a monolayer f
7 f patients with pancreatic cancer, including immunoaffinity and label-free physical attribute-based c
8 combines the selectivity and sensitivity of immunoaffinity and mass spectrometric based techniques f
10 f >10 mg of monomeric hOR17-4 receptor after immunoaffinity and size exclusion chromatography, with e
16 observations demonstrate the utility of CD45 immunoaffinity-based approaches for producing highly pur
17 rochannel and also a technique for improving immunoaffinity-based circulating tumor cell (CTC) captur
23 as isolated through binding of either tag to immunoaffinity beads and then decorated with antibodies
25 cow's milk allergy has been performed using immunoaffinity capillary electrophoresis (IACE) coupled
28 say that uses sequential protein and peptide immunoaffinity capture for protein target quantitation.
30 nd AhR knockdown H358 cells was validated by immunoaffinity capture stable isotope dilution ([(15)N(5
32 pressed in E. coli, and purified by a single immunoaffinity chromatographic step using a monoclonal a
36 fied ISGylated proteins from human thymus by immunoaffinity chromatography and analyzed ISG15 conjuga
37 ctrometry (MS/MS) assay that utilizes online immunoaffinity chromatography and column switching was d
38 , we isolated apo(a)-containing particles by immunoaffinity chromatography and determined the concent
39 ted complexes containing PLTP from plasma by immunoaffinity chromatography and determined their compo
41 he intervention measured by using sequential immunoaffinity chromatography and liquid chromatography-
43 f serpin-6 and PAP-3 in induced hemolymph by immunoaffinity chromatography and mass spectrometry.
45 300 gel filtration chromatography, anti-FLAG immunoaffinity chromatography and SDS/PAGE permitted pur
47 zed receptor was easily purified by one-step immunoaffinity chromatography and the purified receptor
49 as separated by anti-apoC-III and anti-apoE immunoaffinity chromatography and ultracentrifugation in
50 d several mutants, from transfected cells by immunoaffinity chromatography and visualized individual
51 say methods were developed for detection and immunoaffinity chromatography capture was developed for
53 C heterocomplex was isolated and purified by immunoaffinity chromatography from insect cells co-expre
54 is mAb, isolation of the troponin complex by immunoaffinity chromatography from muscle homogenate and
55 hain, cross-linked peptides were isolated by immunoaffinity chromatography from proteolytic digests o
56 on, Inc., Sunnyvale, Calif.) was purified by immunoaffinity chromatography from supernatant fluids of
57 from rod outer segments of bovine retinae by immunoaffinity chromatography in octyl glucoside was rec
60 s of the protein to facilitate separation by immunoaffinity chromatography of overproduced mutant enz
61 e (EEF) tripepetide to allow purification by immunoaffinity chromatography on an immobilized monoclon
64 extracts by a combination of traditional and immunoaffinity chromatography steps and found that the p
66 olution mass spectrometry (MS) combined with immunoaffinity chromatography to determine quantitativel
67 nt signaling, we used cell fractionation and immunoaffinity chromatography to examine the physical en
69 o identify new vaccine targets, we have used immunoaffinity chromatography to isolate class I HLA-A*0
70 pump-generated flow to deliver reagents and immunoaffinity chromatography to isolate the antigen fro
72 nds the powerful technique of gentle-release immunoaffinity chromatography to many expressed proteins
74 control children (n = 54), we used recycling immunoaffinity chromatography to measure the neuropeptid
75 f conventional chromatography and anti-SRCAP immunoaffinity chromatography to purify a native human S
76 and HBeAg, we used these Fabs in microscale immunoaffinity chromatography to purify HBeAg from indiv
77 urified from transformed Escherichia coli by immunoaffinity chromatography using a monoclonal antibod
78 bilized platelet membranes were subjected to immunoaffinity chromatography using an antibody raised a
84 ern blot analyses, immunohistochemistry, and immunoaffinity chromatography were used to detect and pu
85 compartments were seen following pulldown by immunoaffinity chromatography with Rab-specific antibodi
86 roteases inhibited by these serpins, we used immunoaffinity chromatography with serpin antibodies to
87 ication procedure (size, heparin, anion, and immunoaffinity chromatography): two heat shock proteins
88 h the receptor protein using both ligand and immunoaffinity chromatography, 2) TXA2 receptor activati
91 e unlabeled apoB:1000 particles, isolated by immunoaffinity chromatography, contained 56 PL, 8 TAG, a
92 oisolated with PINCH from mammalian cells by immunoaffinity chromatography, indicating that PINCH and
93 usly pure mSR-BI-t1, prepared by single-step immunoaffinity chromatography, mediated high affinity HD
96 n of endogenous levels of (FLAG)betabeta' by immunoaffinity chromatography, which was found to have 0
115 treatment and centrifugation, followed by an immunoaffinity column (IAC) clean-up step before mass sp
117 liquid-liquid extraction, clean-up using an immunoaffinity column (IAC), and identification by rever
118 the types of antibodies that are used in the immunoaffinity column and by using an appropriate detect
119 n in bronchoalveolar lavage and an anti-SNIP immunoaffinity column binds a 70-kDa protein in U937 cel
122 fects of human Abs affinity-purified (AF) by immunoaffinity column chromotography on excitation-contr
124 munoadsorption (EIA) of anti-Gal Ab using an immunoaffinity column of a Gal type 6 oligosaccharide de
125 ter two-dimensional gel electrophoresis, and immunoaffinity column purification, N-terminal amino-aci
131 S method against sample preparation using an immunoaffinity column; the recovery and specificity were
132 Ochratoxin A analyses were performed with immunoaffinity columns and detection by high performance
133 gel filtration and associated with RNAPII on immunoaffinity columns prepared with anti-CTD antibodies
134 C instrumentation after pre-separation using immunoaffinity columns that work through a mechanism of
135 native cleanup procedures (MultiSep and IAC--immunoaffinity columns) was performed, being the advanta
140 observation, we have developed a CD45-based immunoaffinity depletion method for removing CD45-contai
143 ll reproducibility of the process, including immunoaffinity depletion, is high, with a process replic
147 inase ubiquitination using ubiquitin remnant immunoaffinity enrichment and quantitative mass spectrom
151 e methods to characterize the performance of immunoaffinity enrichment of peptides up to multiplex le
152 DAMTS-13 substrate (vWFh) was performed with immunoaffinity enrichment of the reaction substrate and
153 The assay combines magnetic bead-based NGF immunoaffinity enrichment using a non-neutralizing polyc
155 gh-resolution mass spectrometry coupled with immunoaffinity enrichment, we identified 47 lysine-acety
156 alytes and solutions to carry out integrated immunoaffinity extraction and electrophoretic separation
161 whole spot was dissolved prior to cleanup by immunoaffinity extraction, tryptic digest, and preconcen
164 mated using the VICAM AflaTest and OchraTest immunoaffinity fluorometric method in a total of 50 meat
167 s strain behaved as a typical BoNT/B, having immunoaffinity for anti-B monoclonal antibodies and clea
168 from the liver was measured with a sensitive immunoaffinity/gas chromatography/high-resolution mass s
169 is presented, which involves coextraction by immunoaffinity (IA) beads and codetermination by selecte
170 pectrometry (LC/MS/MS) typically utilizes an immunoaffinity (IA) enrichment step such as immunoprecip
172 chromatography/mass spectrometry analysis of immunoaffinity isolates of hippocampal neurons, that RAR
175 ontaining each protein could be separated by immunoaffinity isolation; lectin binding showed that the
176 ion of a pronase digestion step prior to the immunoaffinity LC-MS/MS allowed for measuring not only f
180 tadiene for 90 days, using a newly developed immunoaffinity liquid chromatography tandem mass spectro
190 of 180 ms between the sample and an anti-BSA immunoaffinity microcolumn and provided a signal within
194 we demonstrate using a combination of online immunoaffinity-postconcentration-mass spectrometry in co
195 ic target for HSV-1 infection, and a protein immunoaffinity procedure, we enriched fully glycosylated
197 , we used a discovery platform consisting of immunoaffinity profiling coupled to mass spectrometry th
198 tive phosphorylation of STAT5 and applied an immunoaffinity profiling strategy to identify tyrosine-p
199 can be recognized by specific antibodies for immunoaffinity purification (IAP) and subsequent identif
200 elles containing TrkB isolated from brain by immunoaffinity purification also contain dynein with IC-
201 eract with Kir2 channels, as demonstrated by immunoaffinity purification and affinity chromatography
207 loyed proteomic screening by phosphotyrosine immunoaffinity purification and tandem mass spectrometry
208 the A17 membrane protein was demonstrated by immunoaffinity purification and Western blot analysis.
211 um freudenreichii subsp. shermanii and after immunoaffinity purification in extracts of cereal matric
212 pecific recognition of PEGylated species, an immunoaffinity purification method (IAP) using anti-PEG
217 rategy that incorporates rapid and efficient immunoaffinity purification of Complex I followed by dif
218 tity of proteins analyzed by LC-MS following immunoaffinity purification of IsoLG-modified proteins.
219 -denaturing conditions has made possible the immunoaffinity purification of labile, multisubunit enzy
222 dividual serpin-1 isoforms in plasma we used immunoaffinity purification of serpin-1 isoforms from M.
224 we established that tcTPC allowed stringent immunoaffinity purification of the gamma-secretase compl
225 ed from the infected cells were subjected to immunoaffinity purification of the tagged proteins by ad
227 at represent 3 Bcr-Abl fusion types by using immunoaffinity purification of tyrosine phosphopeptides
228 d signaling intermediates were identified by immunoaffinity purification of tyrosine-phosphorylated p
229 undertaken by two independent approaches: 1) immunoaffinity purification on a mAb raised to condition
230 of 8-oxo-dGuo observed were confirmed by an immunoaffinity purification stable isotope dilution ([(1
232 ibody-induced hearing loss, we used antibody immunoaffinity purification to isolate the IESCA, which
235 cted human embryonal kidney (HEK) 293 cells, immunoaffinity purification, and mass spectrometry combi
236 d characterized by rate-zonal sedimentation, immunoaffinity purification, electron microscopy, and th
238 n tested for immunofluorescence staining and immunoaffinity purification, leading to putative identif
243 To investigate its function further, we immunoaffinity purified a bestrophin complex from RPE ly
244 LA-A*11:01 harvested from infected cells was immunoaffinity purified and acid boiled to release heavy
247 ble complex containing MLL1 and MOF has been immunoaffinity purified from a human cell line that stab
249 UDP-N-acetylglucosamine pyrophosphorylase immunoaffinity purified from encysting and non-encysting
252 d from six independent Mediator preparations immunoaffinity purified through their Nut2 (MED10), Med2
258 cDNA corresponding to the core protein of an immunoaffinity-purified arabinogalactan protein (AGP) se
259 rone assay after immunoprecipitation or with immunoaffinity-purified argpyrimidine-alpha-crystallin i
260 total OCP sera, IgG fractions from OCP sera, immunoaffinity-purified autoantibodies from sera of pati
262 s of these properties and the sufficiency of immunoaffinity-purified epitope-tagged AtPCS1 polypeptid
263 associated with Gbeta5 in the brain, it was immunoaffinity-purified from a nonionic detergent extrac
266 was used to analyze proteins associated with immunoaffinity-purified HDL from plasma obtained from 2
268 es were exposed to various concentrations of immunoaffinity-purified leukotoxin and the cytotoxicity
271 dicate that OCP sera, OCP IgG fractions, and immunoaffinity-purified OCP autoantibodies react with th
273 m a keratinocyte expression library by using immunoaffinity-purified OCP autoantibody, encoded the cy
274 and IgG fractions from 10 patients with OCP; immunoaffinity-purified OCP autoantibody; antibodies to
276 e sequence analysis of a tryptic digest from immunoaffinity-purified Pa showed 100% identity to human
279 from the brain and lung extracts, and in the immunoaffinity-purified sample, but not in the negative
286 The method involved analyte enrichment by immunoaffinity separation using anti-rhEPO antibody link
287 mprised analyte extraction and enrichment by immunoaffinity separation with anti-rhEPO antibodies, du
289 Liquid-solid extraction, clean-up using immunoaffinity solid phase extraction chromatography, an
290 roduced in culture by first incorporating an immunoaffinity step using monoclonal antibodies to purif
295 purified the putative ATPDase from liver by immunoaffinity techniques and obtained a heavily glycosy
296 by density gradient ultracentrifugation and immunoaffinity techniques specific to apolipoprotein B-1
297 boratory to use LC/MS/MS in conjunction with immunoaffinity techniques to evaluate candidate biomarke
300 loped a novel workflow integrating DNA-based immunoaffinity with mass spectrometry to analytically va
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