ライフサイエンスコーパス: immunoblot

1 es against laminin gamma1 were determined by immunoblot.

2 cle protein (PLVAP) were detected by Western immunoblot.

3 the SAN protein was confirmed by Connexin 43 immunoblot.

4 CR, only ABHD12 and ABHD16A were detected by immunoblot.

5 and analyzed in proliferation assays and by immunoblots.

6 inding proteins show reactivity in serum IgE immunoblots.

7 es were analyzed by immunohistochemistry and immunoblots.

8 nal transduction proteins were measured with immunoblots.

9 diseases by ELISA, immunohistochemistry and immunoblots.

10 pha complexes in cells with blue native PAGE immunoblotting.

11 rology consisting of two-dimensional Western-immunoblotting.

12 ation as determined by mass spectrometry and immunoblotting.

13 lected to assess MMP and RANKL production by immunoblotting.

14 and signaling pathways were determined using immunoblotting.

15 y available BACE1 inhibitor, was verified by immunoblotting.

16 Conversion of LC3 was further validated by immunoblotting.

17 ive or equivocal, is followed by second-tier immunoblotting.

18 cryptosporidiosis through immunostaining and immunoblotting.

19 were studied by immunohistochemistry and by immunoblotting.

20 s (SPTs), specific IgEs (sIgEs) and SDS-PAGE immunoblotting.

21 B, and Erk1,2 phosphorylation detectable by immunoblotting.

22 lasts from patients, which were confirmed by immunoblotting.

23 racted from the seeds when examined with IgE immunoblotting.

24 downstream signalling events with ELISA and immunoblotting.

25 ns by qRT-PCR, and protein concentrations by immunoblotting.

26 ression was confirmed through microscopy and immunoblotting.

27 e and polyacrylamide gel electrophoresis and immunoblotting.

28 olecules and to S. aureus and E. coli by IgE immunoblotting.

29 er tissues were analyzed by histology and by immunoblotting.

30 ppocampi and spinal cords were collected for immunoblotting.

31 the downstream targets of HDAC4 knockdown by immunoblotting.

32 ied by enzyme-linked immunosorbent assay and immunoblotting.

33 regulation of RPE phagocytosis, confirmed by immunoblot analyses and in vitro phagocytosis assays.

34 Immunohistochemical, immunocytochemical, and immunoblot analyses and transfection, infection, DNA syn

35 Immunohistochemical and immunoblot analyses demonstrated that the HIPPO central

36 rase chain reaction, immunofluorescence, and immunoblot analyses in Caco-2, HT-29, and T-84 cells hum

37 We performed proteomic and immunoblot analyses of attenuated L. major strains defic

38 epatoma cells and confirmed its depletion by immunoblot analyses of purified viral particles.

39 x in melon fruit, as shown by carotenoid and immunoblot analyses of selected melon genotypes and by u

40 Immunoblot analyses of soluble protein extracts revealed

41 Electron microscopy and immunoblot analyses showed significantly higher levels o

42 Immunoblot analyses showed these four proteins had highe

43 n polymerase chain reaction, sequencing, and immunoblot analyses.

44 emical, immunofluorescence, biochemical, and immunoblot analyses.

45 quantitative polymerase chain reaction, and immunoblot analyses.

46 ins were localized by immunofluorescence and immunoblot analyses.

47 clear extracts followed by mass spectrometry-immunoblotting analyses revealed that, upon TSEC treatme

48 Using real-time RT-PCR and immunoblotting analyses, we measured mRNA expressions an

49 ll roasted peanuts was increased as shown by immunoblot analysis and BAT.

50 Immunoblot analysis and chromatin immunoprecipitation of

51 se cardiomyocytes compared with wild type by immunoblot analysis and confocal immunofluorescence micr

52 His-tagged CesA5 from Physcomitrella patens Immunoblot analysis and mass spectrometry confirmed enri

53 In vivo, immunoblot analysis demonstrated that nephrin expression

54 Immunoblot analysis of fresh frozen brain tissues reveal

55 METHODS AND Immunoblot analysis of myocardium from end-stage HF pati

56 scence associated with autophagic bodies and immunoblot analysis of the ATG8 protein to show that sul

57 Confocal microscopy and immunoblot analysis of the hippocampus showed progressiv

58 o, whilst qPCR, Taqman low-density array and immunoblot analysis of these tissues further revealed in

59 the Nr1h4 promoter in wild-type kidneys, and immunoblot analysis revealed downregulated expression of

60 Immunoblot analysis showed that the elevation of PSS act

61 By qPCR and immunoblot analysis we verified that the nuclear PSA-car

62 Gel electrophoresis and immunoblot analysis were used to determine protein stabi

63 -transcriptase polymerase chain reaction and immunoblot analysis were used to measure messenger RNA a

64 Immunoblot analysis with a panel of amyloid-beta antibod

65 On immunoblot analysis, fibroblasts grown from shagreen pat

66 e in EC were confirmed by immunostaining and immunoblot analysis, respectively.

67 alyzed by SDS-PAGE, native PAGE, and Western immunoblot analysis.

68 ot activated when evaluated by multiplex and immunoblot analysis.

69 concentration using confocal microscopy and immunoblot analysis.

70 ut advanced liver disease were determined by immunoblot analysis.

71 measured by flow cytometry and confirmed by immunoblot analysis.

72 Our qRT-PCR and immunoblotting analysis revealed that reduced levels of

73 Immunoblotting analysis revealed that sirtinol significa

74 Immunoblotting analysis showed that Kif2a was gradually

75 lenge to argan powder, skin prick tests, and immunoblotting analysis.

76 were collected for quantitative RT-PCR, and immunoblot and hydroxyproline release assays.

77 oms were screened for alpha-gal epitopes via immunoblot and in comparison with cetuximab and pork kid

78 Immunoblot and MALDI-MS/MS analyses revealed that IgE fr

79 d control cohorts using gel electrophoresis, immunoblot and mass spectrometry.

80 serum EVs were isolated and characterized by immunoblot and nanoparticle-tracking analyses.

81 We used immunoblot and quantitative polymerase chain reaction to

82 We then used immunoprecipitation followed by immunoblot and the proximity ligation assay to confirm t

83 ering RNA (siRNA), immunoprecipitation (IP), immunoblots and bioinformatics.

84 on 1 (STAT1) phosphorylation was measured by immunoblots and confocal imaging.

85 Immunoblots and electron microscopy immunolocalization r

86 lactofuranose-containing glycans as shown by immunoblots and immuno-electronmicroscopy employing a ga

87 li or control agents; cells were analyzed by immunoblots and quantitative polymerase chain reaction.

88 Interestingly, by immunoblotting and confocal fluorescence microscopy, we

89 Immunoblotting and confocal microscopy results showed th

90 .1 cells (P < 0.05, n = 3), as determined by immunoblotting and densitometry.

91 bovine gamma globulin (BGG) was assessed by immunoblotting and ELISA, respectively.

92 Immunoblotting and enzyme-linked immunosorbent assay stu

93 phages was investigated by quantitative PCR, immunoblotting and flow cytometry.

94 f 1, Der p 2 and Der f 2 was carried out by immunoblotting and fluorescent multiplex.

95 K8-null, and K18-null mice were analyzed by immunoblotting and immunofluorescence staining of cell a

96 tative polymerase chain reaction followed by immunoblotting and immunofluorescence.

97 protein, in activated macrophages using both immunoblotting and immunofluorescent microscopy.

98 The GluN2D subunit was detectable by immunoblotting and immunohistochemistry in all subfields

99 rformed in transfection experiments by using immunoblotting and immunoprecipitation in STAT1-deficien

100 Our immunoblotting and immunostaining analyses revealed that

101 Using real-time RT-PCR, immunoblotting and immunostaining analyses, we measured

102 In addition, immunoblotting and immunostaining revealed an upregulati

103 We conducted immunoblotting and in vivo microdialysis procedures in M

104 we assessed NEMO linear ubiquitination using immunoblotting and investigated the formation of NEMO-co

105 the Luminex Multiplex assay, ELISA, PCR, and immunoblotting and linked to the presence of EETs.

106 Using imaging, quantitative immunoblotting and mass spectrometry, we show that hundr

107 s of DNA damage and cell-cycle regulators by immunoblotting and performed siRNA-mediated gene silenci

108 expression of TSHR in thymocytes by protein immunoblotting and quantitative PCR, and show that expre

109 Using Western immunoblotting and quantitative polymerase chain reactio

110 podocytes was confirmed in cultured cells by immunoblotting and quantitative real-time PCR and in mou

111 Immunoblotting and selective reaction monitoring were us

112 1 and phosphoglycerate mutase were used with immunoblotting and sequencing IgG variable domains to sc

113 EGFR and RELA was validated by both qPCR and immunoblotting and they were both shown to be under dire

114 e impact of the mutation was investigated by immunoblotting and transcriptome sequencing.

115 two EIA strategies were followed by Western immunoblotting and when used in an MTTT, respectively.

116 and quantitative polymerase chain reaction, immunoblot, and chromatin immunoprecipitation assays.

117 ic procedures (basophil activation test, IgE immunoblot, and experimental ImmunoCAP).

118 is, flow cytometry, and immunohistochemical, immunoblot, and functional assays.

119 es, including immunofluorescence microscopy, immunoblot, and microplate immunoassay are essentially n

120 sted at 0 and 1 month by microscopy, PCR and immunoblot, and serology at 6 and 9 months.

121 ononuclear cells were analyzed by histology, immunoblots, and confocal microscopy.

122 RT-PCR, protein immunoblots, and in vitro plasmablast differentiation as

123 were analyzed by histologic immunostaining, immunoblots, and proliferation assays.

124 We also used immunoprecipitation assays, immunoblotting, and an in situ proximity ligation assay

125 t and feed varieties using quantitative PCR, immunoblotting, and enzyme-linked immunosorbent assay (E

126 s by using flow cytometry, confocal imaging, immunoblotting, and fluorimetric assays.

127 We show by immunofluorescence, immunoblotting, and glycosylation analysis that the vari

128 rter gene expression level was confirmed via immunoblotting, and histological staining was used to id

129 rse transcriptase-polymerase chain reaction, immunoblotting, and immunofluorescence.

130 Protein differences were validated by immunoblotting, and proteins were mapped for biological

131 gel-based and shotgun proteomics, 1D and 2D immunoblotting, and quantitative ELISA were applied.

132 positive or equivocal is reflexed to Western immunoblotting as the second tier.

133 ease or accompanied by a positive IgG or IgM immunoblot assay for Borrelia burgdorferi--to receive a

134 icity of the autoantibodies was confirmed by immunoblot assay, binding to contactin-1-transfected hum

135 lot technique and a semiquantitative Western immunoblot assay.

136 -N-acetylglucosamine (PNAG)-like material by immunoblot assay.

137 in hippocampus and cortex were detected with immunoblotting assay.

138 Immunoblot assays for platelet-derived phosphorylated-eN

139 Immunoblot assays further verified that the virulence ge

140 Immunoblot assays with rabbit anti-ATCV-1 antibody detec

141 Allergenicity of rChiA was verified by immunoblot assays with sera from allergic subjects.

142 S), which were analyzed in proliferation and immunoblot assays, or injected subcutaneously into nude

143 Using IP followed by immunoblot assays, we have developed a validated reposit

144 croscopy, thioflavin-T binding, seeding, and immunoblot assays.

145 in reaction, protein levels were analyzed by immunoblot assays.

146 ription polymerase chain reaction (qPCR), or immunoblot assays; fluorescence-activated cell sorting w

147 Immunoblots assessed G-protein-coupled receptor recyclin

148 recombinant prion protein substrates and/or immunoblot band profiles of proteinase K-treated RT-QuIC

149 In immunoblots, BGG was the most frequently recognized meat

150 eas all antibodies recognized Siglec-E-Fc on immunoblots, binding was dependent on intact disulfide b

151 n addition, the signal-to-noise ratio of the immunoblotting by TDCS can be markedly increased.

152 By immunoblotting, CD36 but not cartilage intermediate laye

153 Studies by using dot blotting, immunoblotting, circular dichroism spectroscopy, and tra

154 Immunoblotting confirmed higher abundances of the select

155 Luciferase-expressing plasmid constructs and immunoblotting confirmed several predicted miRNA targets

156 Western immunoblotting confirmed the production of: LFN-GAL4, LF

157 The prevalence of immunoblot-confirmed B burgdorferi IgG seropositivity in

158 sing a combination of patch clamp recording, immunoblotting, confocal imaging and structural modellin

159 itochondrial matrix volume is determined via immunoblotting, confocal microscopy of intact cells, and

160 Western immunoblots demonstrated decreased free ubiquitin in air

161 Instead, immunoblotting demonstrated proteoforms of ADAM8 that la

162 Immunoblotting demonstrated that SLT11 (pCZ1) and SLT12

163 RNA sequencing and immunoblotting demonstrated that the risk allele locally

164 ll among the three fractions tested, with 1D immunoblots demonstrating a slight predominance of IgE r

165 allergy diagnostics and a newly established immunoblot diagnostic system.

166 oxidation and cell proliferation analysed by immunoblotting did not show differences between BT and n

167 Genome-wide mRNA sequencing, immunoblot, electron microscopy, together with immunoflu

168 analyzed by histology, immunohistochemical, immunoblot, enzyme-linked immunosorbent assay, and quant

169 tive reverse transcriptase-PCR (qRT-PCR) and immunoblot experiments demonstrated direct interaction o

170 In quantitative immunoblotting experiments of STAT proteins, STAT1alpha,

171 Immunoblotting findings of mitochondrial and synaptic pr

172 hese antibodies for the detection of IRF5 by immunoblot, flow cytometry, and immunofluorescence or im

173 tural proteins (E1, E2, and C), reflected by immunoblot fluorescent signals.

174 th specific IgE to alpha-Gal was screened by immunoblot for IgE-reactive proteins in pork kidney.

175 mRNA (RT-PCR) and protein (immunoblotting) for OAT1, OAT3, NaDC3, and MRP4 were det

176 M Glu, fractions 1-10 was reused as the PKM2 immunoblot from 1 mM Glu, fractions 1-10.

177 The PKM2 immunoblot from 5 mM Glu, fractions 1-10 was reused as t

178 The actin immunoblot from A549 cells from Fig 5A was reused as the

179 Immunoblotting from fibroblasts and myoblasts of an affe

180 of anabolic signalling (e.g. Akt/mTORC1) by immunoblotting from muscle biopsies.

181 Parallel use of flow cytometry and immunoblotting further enabled us to estimate the densit

182 ucture of recombinant CLEC3A by SDS-PAGE and immunoblot, glycan analysis, matrix-assisted laser desor

183 on of complementary DNA ends, Southern blot, immunoblot, histologic, immunohistochemical, immunofluor

184 itative real-time polymerase chain reaction, immunoblotting, histological and immunological staining,

185 s panel was tested with two reference tests, immunoblotting (IB) and neutralization (Nt), and with 8

186 Western immunoblots identified shifts in ubiquitin pools.

187 lected specimens, followed by a supplemental immunoblot if the C6 EIA result was positive but the who

188 Using immunoblotting, imaging and mass spectrometry, we use ou

189 Immunoblotting, immunocytochemistry (ICC), and functiona

190 phingolipids and cholesterol and analyzed by immunoblot, immunofluorescence, and reverse transcriptio

191 sed for studies including toxicant exposure, immunoblotting, immunofluorescence analysis, tight junct

192 NA complexes, but not free topo I or DNA, by immunoblotting, immunofluorescence or flow cytometry.

193 nvolved mitochondrial function; results from immunoblotting, immunofluorescence, and functional assay

194 oids in both cohorts were compared by ELISA, immunoblot, immunohistochemistry and real-time PCR.

195 rse-transcription polymerase chain reaction, immunoblot, immunohistochemistry, enzyme-linked immunoso

196 rial function and autophagy were assessed by immunoblotting, immunohistochemistry, and qPCR.

197 on and livers were collected and analyzed by immunoblotting, immunohistochemistry, histology, and rea

198 and mRNA abundance was analyzed by means of immunoblotting, immunohistochemistry, immunofluorescence

199 cells and transfected HEK293T cells through immunoblotting, immunohistochemistry, luciferase activit

200 Addition of a supplemental immunoblot improved the specificity of the C6 EIA to 98.

201 recombinant laminin gamma1 were detected by immunoblot in 8 of 12 patients.

202 The PKM2 immunoblot in Fig 2E was reused as part of the Caspase-3

203 n Fig 2E was reused as part of the Caspase-3 immunoblot in Fig 9C.

204 ted repression of GMPS could be validated by immunoblotting in Sk-Hep1, HepG2, and HuH6 cells.

205 Validations were by immunoblotting in systemic and intracoronary blood from

206 Using immunoblotting, in situ zymography, and immunofluorescen

207 igitoxin followed by immunoprecipitation and immunoblotting indicated that DARPP-32 has an important

208 clusters of differentiation 9, 63, and 81 by immunoblot) indicated that most serum EV were exosomes.

209 e IgE-reactive antigens were detected by IgE immunoblot inhibition experiments.

210 nce that a 2-EIA-based MTTT algorithm, where immunoblotting is replaced by the C6 EIA, performs as we

211 By immunoblotting, kidney cortex homogenate from patients t

212 We applied biatrial optical mapping and immunoblot mapping of various atrial regions to reveal t

213 Analysis by immunoblotting, metabolic labeling, and mass spectrometr

214 Using biochemical and immunoblotting methods, mitochondrial function and phosp

215 ardiomyocytes (CMNCs) and mouse hearts using immunoblotting, molecular imaging, and [(35)S]methionine

216 sing confocal immunofluorescence microscopy, immunoblotting, myeloperoxidase-DNA complex ELISA, and f

217 tive RT-PCR; protein content was measured by immunoblotting; NOS activity was evaluated with high-per

218 d caspase-3, and extravascular albumin), and immunoblotting (nuclear factor-kappaB, hypoxia-inducible

219 DCS in vitro or tDCS in vivo were tested by immunoblot of protein extracted from stimulated slices o

220 significantly altered mobility in denatured immunoblots of CaValpha2delta1 G1060I and CaValpha2delta

221 rmore, the strong IgE reactivity detected in immunoblots of plant-food extracts indicated that Pru p

222 Comprehensive quantitative immunoblotting of protein extracts from human embryonic

223 Alpha-gal epitopes were detected by immunoblotting on antivenoms.

224 Immunoreactivity was evaluated by means of immunoblot or ELISA inhibition assay.

225 for choline/methyl metabolite measurements, immunoblotting or gene expression of relevant enzymes.

226 rse-transcription polymerase chain reaction, immunoblot, or immunofluorescence analyses.

227 analyzed by histology, immunohistochemistry, immunoblots, or ELISAs (to measure cytokines).

228 as analyzed by using phospho flow cytometry, immunoblotting, or co-immunoprecipitation in CD19-defici

229 xpression of signaling molecules by means of immunoblotting, PGD2 and macrophage inflammatory protein

230 ids were cultured and analyzed by histology, immunoblots, phalloidin staining, immunohistochemistry,

231 mug/mL), or both (CSE+EtOH), and analyzed by immunoblotting, quantitative reverse-transcription polym

232 aphy and LC-MS/MS) and immunological (ELISA, immunoblot, RBL-assays, animal model) analysis showed a

233 h quantitative polymerase chain reaction and immunoblotting, respectively.

234 tative polymerase chain reaction and Western immunoblotting, respectively.

235 Immunoblots revealed increased levels of proNGF in AD su

236 Immunoblots revealed that the expression level of Nogo-A

237 Immunoblotting revealed downregulation of the medullary

238 In the XLH cultures, immunoblotting revealed more abundant osteopontin (OPN),

239 Immunoblotting revealed rab17 immunoreactive species at

240 icroarray analysis, quantitative RT-PCR, and immunoblotting revealed that IRF2 regulated the basal le

241 Immunoblotting revealed that this was associated with ac

242 cal decision making, although a supplemental immunoblot should still be performed.

243 In genipin-treated TM cells, Western immunoblotting showed a reduction of active MMP2 and MMP

244 fate poly acrylamide gel electrophoresis and immunoblotting showed acid-soluble collagen fraction fro

245 Western immunoblotting showed increases of the iron storage prot

246 N-beta than was the wild-type S. aureus, and immunoblotting showed that IFN-beta interacts with the b

247 Immunoblotting showed that the superior/middle lateral R

248 The diagnosis was confirmed by immunoblot showing autoantibodies against 200-kDa protei

249 The immunoblot signal strength to C and the ratio of the C s

250 signaling 3 protein levels were measured by immunoblot, STAT3-TGFB1 DNA-binding activity by chromati

251 Here, with the help of an immunoblotting strategy and Ime4-GFP protein localizatio

252 Targeted LC-MS/MS and immunoblotting studies further confirmed the down regula

253 Moreover, immunoblotting studies of selectively bred and transgeni

254 Immunoblotting suggested that SerpinB2 (cross-linked int

255 sphorylation was detected by two-dimensional immunoblotting, suggesting that loss of SMA-5 function l

256 Immunoblotting suggests that two classes of phosphorylat

257 chromatography, and nondenaturing/denaturing immunoblotting techniques.

258 reeze-dried in order to perform SDS-PAGE and immunoblotting tests.

259 gy reacted more frequently to tropomyosin in immunoblots than did patients without it (93% vs 35%, re

260 icroscopy, immunohistochemistry, and Western immunoblotting to examine the morphology and microstruct

261 We applied quantitative immunoblotting to generate profiles of transporters, cha

262 ss-linking followed by mass spectrometry and immunoblotting to isolate and characterize proteins comp

263 human serum IgG4 were identified by means of immunoblotting to screen for potential bacterial allerge

264 We employed affinity purification and immunoblotting to validate the interaction between STAT5

265 Da) were detected in pork kidney extracts by immunoblot using patient sera and an anti-alpha-Gal anti

266 Immunoblotting using an Ser-235 phosphorylation-specific

267 tin modifications were determined by Western immunoblotting using specific antibodies.

268 he same samples and therefore the beta-actin immunoblot was reused.

269 IgE-immunoblotting was also conducted using sera from childr

270 Immunoblotting was employed on tissue from subregions of

271 IgE immunoblotting was performed with pooled sera from food-

272 transcripts by qPCR and protein analysis via immunoblotting was performed.

273 -linked immunosorbent assay and confirmed by immunoblot) was performed were retrospectively reviewed.

274 telets followed by validation via RT-PCR and immunoblot, we discovered that granzyme A (GrmA), a seri

275 ipitation coupled with mass spectrometry and immunoblots, we discovered that inactive, unphosphorylat

276 Using (32)P-labeled NAD(+) and immunoblotting, we also demonstrate that both subunits o

277 rm of TBCD and using non-denaturing gels and immunoblotting, we analyzed lysates from a number of mou

278 In addition, through immunoblotting, we found that AHAs overexpressed the NMD

279 confirmation by pulldown assays followed by immunoblotting, we identified P-selectin and multimerin-

280 uantitative RT-PCR, immunohistochemistry and immunoblots were carried out to confirm that changes in

281 2D immunoblots were made in parallel to elucidate IgE and I

282 Western immunoblots were performed with specific antibodies to A

283 To assess additional GLUTs, immunoblots were performed.

284 Immunoblots were used to assess the underlying mechanism

285 Some lanes in the immunoblots were used to represent different experimenta

286 extraction, the MNase sensitivity assay, and immunoblotting were used to assess global histone acetyl

287 lectron microscopy, histologic analyses, and immunoblotting were used to determine the effects of ATP

288 Immunofluorescence and immunoblotting were utilized to detect surface-localized

289 hm and modified criteria for second-tier IgG immunoblots, were also evaluated.

290 easured via direct kinase activity assay and immunoblotting, whereas genes related to mitochondrial b

291 rmed and diagnosis of anti-p200 confirmed by immunoblot with dermal extract.

292 mpact of thermal processing were analyzed by immunoblot with sera from 52 peanut-allergic individuals

293 ayed decreased phospho-signal intensities in immunoblotting with anti-phosphoserine/phosphothreonine

294 caspase-9 (CC-9) expression were assayed by immunoblotting with or without SIRT3 enforced expression

295 -polyacrylamide gel electrophoresis SDS-PAGE-immunoblotting with patient sera and rabbit serum anti-P

296 IgE-immunoblotting with peach leaf extract revealed in six p

297 Immunocapture of F0F1-ATPase and immunoblotting with phospho(Ser) PKC substrate antibody

298 Proteins were studied by using SDS-PAGE and immunoblotting with sera from patients with peanut aller

299 s by iTRAQ/LC-MS/MS with TiO2 enrichment and immunoblotting with specific anti-phospho-NHE3 antibodie

300 A alone and as a first-tier test followed by immunoblot, with that of standard 2-tiered serology for