ライフサイエンスコーパス: immunoblotting

1 Conversion of LC3 was further validated by immunoblotting.

2 ive or equivocal, is followed by second-tier immunoblotting.

3 cryptosporidiosis through immunostaining and immunoblotting.

4 were studied by immunohistochemistry and by immunoblotting.

5 s (SPTs), specific IgEs (sIgEs) and SDS-PAGE immunoblotting.

6 B, and Erk1,2 phosphorylation detectable by immunoblotting.

7 lasts from patients, which were confirmed by immunoblotting.

8 racted from the seeds when examined with IgE immunoblotting.

9 downstream signalling events with ELISA and immunoblotting.

10 ression was confirmed through microscopy and immunoblotting.

11 ns by qRT-PCR, and protein concentrations by immunoblotting.

12 CXCL2 and CXCR2 were confirmed by ELISA and immunoblotting.

13 l and conventional fluorescence imaging, and immunoblotting.

14 of gene expression, quantitative RT-PCR, and immunoblotting.

15 against the same bacteria using checkerboard immunoblotting.

16 quantitative polymerase chain reaction, and immunoblotting.

17 ically developed/validated ELISA test and by immunoblotting.

18 e and polyacrylamide gel electrophoresis and immunoblotting.

19 the natural CPSA by (1)H NMR, (31)P NMR, and immunoblotting.

20 GFAP, ZO-1, and occludin were determined by immunoblotting.

21 oproteinases and aggrecanases as revealed by immunoblotting.

22 ic antibodies against NIH-CQV were sought by immunoblotting.

23 xes whose constituents were characterized by immunoblotting.

24 demonstrated in degenerate disc tissues via immunoblotting.

25 ived synapses, 22 of which were validated by immunoblotting.

26 ermined by quantitative RT-PCR (qRT-PCR) and immunoblotting.

27 inase expression were determined by means of immunoblotting.

28 , and antioxidant enzymes were determined by immunoblotting.

29 roteins and phosphorylation of AMPKalpha2 by immunoblotting.

30 harvested for Brn3b immunostaining and BDNF immunoblotting.

31 ays, confocal microscopy, real-time PCR, and immunoblotting.

32 0) were examined by immunohistochemistry and immunoblotting.

33 GST+Alr1105 fusion protein was confirmed by immunoblotting.

34 olecules and to S. aureus and E. coli by IgE immunoblotting.

35 er tissues were analyzed by histology and by immunoblotting.

36 ppocampi and spinal cords were collected for immunoblotting.

37 the downstream targets of HDAC4 knockdown by immunoblotting.

38 ied by enzyme-linked immunosorbent assay and immunoblotting.

39 pha complexes in cells with blue native PAGE immunoblotting.

40 rology consisting of two-dimensional Western-immunoblotting.

41 ation as determined by mass spectrometry and immunoblotting.

42 lected to assess MMP and RANKL production by immunoblotting.

43 and signaling pathways were determined using immunoblotting.

44 y available BACE1 inhibitor, was verified by immunoblotting.

45 lysosomotropic agents, proliferation assays, immunoblotting, a Pgp-ATPase activity assay, radiolabele

46 2D electrophoresis and immunoblotting also provided evidence for the presence o

47 As revealed by immunoblotting, anaerobic conditions that lead to the in

48 clear extracts followed by mass spectrometry-immunoblotting analyses revealed that, upon TSEC treatme

49 Immunoblotting analyses were conducted to evaluate expre

50 Using real-time RT-PCR and immunoblotting analyses, we measured mRNA expressions an

51 Immunoblotting analysis demonstrated that non-degenerate

52 Our qRT-PCR and immunoblotting analysis revealed that reduced levels of

53 Immunoblotting analysis revealed that sirtinol significa

54 Immunoblotting analysis showed that Kif2a was gradually

55 identify sphingomyelin species coupled with immunoblotting analysis to comprehensively map differenc

56 lenge to argan powder, skin prick tests, and immunoblotting analysis.

57 orm of C5aR (cC5aR) was detected in serum by immunoblotting and a flow-based capture assay, suggestiv

58 Microarray data were further validated by immunoblotting and by cholesterol and protein-thiol oxid

59 Interestingly, by immunoblotting and confocal fluorescence microscopy, we

60 Immunoblotting and confocal microscopy results showed th

61 .1 cells (P < 0.05, n = 3), as determined by immunoblotting and densitometry.

62 bovine gamma globulin (BGG) was assessed by immunoblotting and ELISA, respectively.

63 Contemporary techniques, such as immunoblotting and enzyme immunoassays, require extensiv

64 Immunoblotting and enzyme-linked immunosorbent assay stu

65 phages was investigated by quantitative PCR, immunoblotting and flow cytometry.

66 f 1, Der p 2 and Der f 2 was carried out by immunoblotting and fluorescent multiplex.

67 Immunoblotting and histological staining confirmed the e

68 IgE-binding proteins were detected by immunoblotting and identified by mass spectrometry.

69 l/soybean/hazelnut extracts were analyzed by immunoblotting and immunoblot-inhibition studies.

70 Proteins were detected and identified by immunoblotting and immunocytochemistry.

71 K8-null, and K18-null mice were analyzed by immunoblotting and immunofluorescence staining of cell a

72 Cdc42 expression was analyzed by immunoblotting and immunofluorescence.

73 tative polymerase chain reaction followed by immunoblotting and immunofluorescence.

74 zed a new REEP1 monoclonal antibody for both immunoblotting and immunofluorescent microscopic analysi

75 protein, in activated macrophages using both immunoblotting and immunofluorescent microscopy.

76 Immunoblotting and immunohistochemical analyses of HIV-1

77 ospho-Ser-381 melanopsin, we demonstrated by immunoblotting and immunohistochemical staining in HEK-2

78 The antibody specificity was verified by immunoblotting and immunohistochemical staining of HEK-2

79 The GluN2D subunit was detectable by immunoblotting and immunohistochemistry in all subfields

80 tional resistivity and quantitative connexin immunoblotting and immunohistochemistry.

81 rformed in transfection experiments by using immunoblotting and immunoprecipitation in STAT1-deficien

82 Our immunoblotting and immunostaining analyses revealed that

83 Using real-time RT-PCR, immunoblotting and immunostaining analyses, we measured

84 In addition, immunoblotting and immunostaining revealed an upregulati

85 tween quercetin and hnRNPA1 was validated by immunoblotting and in vitro binding experiments.

86 itro for immunocytometry, histopathology and immunoblotting and in vivo for image-guided surgery.

87 We conducted immunoblotting and in vivo microdialysis procedures in M

88 ntibodies detected by ELISA and confirmed by immunoblotting and indirect immunofluorescence.

89 additional techniques consisting of Western immunoblotting and infrared spectroscopy, and 4 distinct

90 we assessed NEMO linear ubiquitination using immunoblotting and investigated the formation of NEMO-co

91 the Luminex Multiplex assay, ELISA, PCR, and immunoblotting and linked to the presence of EETs.

92 is of key proteins from these pathways using immunoblotting and live cell microscopy.

93 Immunoblotting and luciferase reporter assays confirmed

94 Using imaging, quantitative immunoblotting and mass spectrometry, we show that hundr

95 calization was conclusively shown by Western immunoblotting and MS of nuclear lysate proteins.

96 Immunoblotting and N-terminal amino acid sequence analys

97 s of DNA damage and cell-cycle regulators by immunoblotting and performed siRNA-mediated gene silenci

98 re, we further characterize this complex via immunoblotting and proteomic analysis of tandem affinity

99 expression of TSHR in thymocytes by protein immunoblotting and quantitative PCR, and show that expre

100 Using Western immunoblotting and quantitative polymerase chain reactio

101 E3 ligases, and autophagy were measured with immunoblotting and quantitative polymerase chain reactio

102 ological medium in vitro as measured by both immunoblotting and quantitative proteomics were not rest

103 podocytes was confirmed in cultured cells by immunoblotting and quantitative real-time PCR and in mou

104 In vitro viral infection was confirmed by immunoblotting and radioiodine uptake assays.

105 d the allergenic property by an IgE-specific immunoblotting and rat basophil leukemia cell (RBL-SX38)

106 ith up-regulation of Bnip3, as determined by immunoblotting and real-time polymerase chain reaction (

107 Immunoblotting and selective reaction monitoring were us

108 1 and phosphoglycerate mutase were used with immunoblotting and sequencing IgG variable domains to sc

109 Immunoblotting and siRNA studies confirmed the NFkappaB-

110 sian men, by employing immunohistochemistry, immunoblotting and Slot-blotting.

111 T47-D epithelial breast cancer cells by both immunoblotting and the direct determination of total glu

112 EGFR and RELA was validated by both qPCR and immunoblotting and they were both shown to be under dire

113 Immunoblotting and transcriptional studies revealed that

114 e impact of the mutation was investigated by immunoblotting and transcriptome sequencing.

115 two EIA strategies were followed by Western immunoblotting and when used in an MTTT, respectively.

116 otein 1 expression (about 3-fold increase on immunoblotting), and promoted lipid accumulation in huma

117 We also used immunoprecipitation assays, immunoblotting, and an in situ proximity ligation assay

118 recombinant protein were tested by ELISA and immunoblotting, and biological activity was tested accor

119 t and feed varieties using quantitative PCR, immunoblotting, and enzyme-linked immunosorbent assay (E

120 s by using flow cytometry, confocal imaging, immunoblotting, and fluorimetric assays.

121 We show by immunofluorescence, immunoblotting, and glycosylation analysis that the vari

122 rter gene expression level was confirmed via immunoblotting, and histological staining was used to id

123 le) of these individuals via flow cytometry, immunoblotting, and immunocytochemistry.

124 H) outflow pathway was determined by RT-PCR, immunoblotting, and immunofluorescence.

125 rse transcriptase-polymerase chain reaction, immunoblotting, and immunofluorescence.

126 Quantitative RT-PCR, immunoblotting, and immunohistochemistry experiments sho

127 cription polymerase chain reaction (RT-PCR), immunoblotting, and immunohistochemistry were used to ex

128 xicity were determined using flow cytometry, immunoblotting, and immunohistochemistry.

129 Protein differences were validated by immunoblotting, and proteins were mapped for biological

130 gel-based and shotgun proteomics, 1D and 2D immunoblotting, and quantitative ELISA were applied.

131 Immunohistochemistry, immunoblotting, and reporter assays all show a significa

132 red by real-time quantitative RT-PCR, ELISA, immunoblotting, and zymography, respectively.

133 ophoresis, isoelectric focusing (IEF)-double immunoblotting, aptamer/antibody-based methods, and late

134 positive or equivocal is reflexed to Western immunoblotting as the second tier.

135 in hippocampus and cortex were detected with immunoblotting assay.

136 products were typically 50-90%, as judged by immunoblotting, but in no case did the activity of Compl

137 n addition, the signal-to-noise ratio of the immunoblotting by TDCS can be markedly increased.

138 By immunoblotting, CD36 but not cartilage intermediate laye

139 Immunoblotting, cell counting, and neutral lipid stainin

140 Studies by using dot blotting, immunoblotting, circular dichroism spectroscopy, and tra

141 Immunoblotting confirmed higher abundances of the select

142 Luciferase-expressing plasmid constructs and immunoblotting confirmed several predicted miRNA targets

143 Western immunoblotting confirmed the production of: LFN-GAL4, LF

144 sing a combination of patch clamp recording, immunoblotting, confocal imaging and structural modellin

145 itochondrial matrix volume is determined via immunoblotting, confocal microscopy of intact cells, and

146 -transcriptase polymerase chain reaction and immunoblotting data showed that in the absence of CHOP,

147 Instead, immunoblotting demonstrated proteoforms of ADAM8 that la

148 Immunoblotting demonstrated that SLT11 (pCZ1) and SLT12

149 RNA sequencing and immunoblotting demonstrated that the risk allele locally

150 oxidation and cell proliferation analysed by immunoblotting did not show differences between BT and n

151 Immunoblotting, ELISAs, and ELISA inhibition assays were

152 mass spectrometry, concanavalin A detection, immunoblotting, enzyme-linked immunosorbent assays, baso

153 Immunoblotting, established that glycosylation is not re

154 rization status by cross-linking followed by immunoblotting, evaluated the ability of the Puma BH3 do

155 In quantitative immunoblotting experiments of STAT proteins, STAT1alpha,

156 High-resolution IgE immunoblotting experiments revealed that 50 (54%) of 92

157 Immunoblotting findings of mitochondrial and synaptic pr

158 Immunoblotting findings of mitochondrial and synaptic pr

159 nd Gli2) induced autophagy, as determined by immunoblotting for microtubule-associated protein light

160 platelets or their releasates, as judged by immunoblotting for phospho-amino acids and PS.

161 mRNA (RT-PCR) and protein (immunoblotting) for OAT1, OAT3, NaDC3, and MRP4 were det

162 Immunoblotting from fibroblasts and myoblasts of an affe

163 of anabolic signalling (e.g. Akt/mTORC1) by immunoblotting from muscle biopsies.

164 Parallel use of flow cytometry and immunoblotting further enabled us to estimate the densit

165 sections, and by additional methods in rats (immunoblotting, histochemistry, and electron microscopy)

166 itative real-time polymerase chain reaction, immunoblotting, histological and immunological staining,

167 s panel was tested with two reference tests, immunoblotting (IB) and neutralization (Nt), and with 8

168 uorescence and low-density membrane fraction immunoblotting); iii) increased claudin 2 expression at

169 Using immunoblotting, imaging and mass spectrometry, we use ou

170 Immunoblotting, immunocytochemistry (ICC), and functiona

171 erized probe specificity and bioactivity via immunoblotting, immunocytochemistry, and flow cytometric

172 We found (using TaqMan RT-PCR, immunoblotting, immunocytochemistry, and proteome analys

173 sed for studies including toxicant exposure, immunoblotting, immunofluorescence analysis, tight junct

174 NA complexes, but not free topo I or DNA, by immunoblotting, immunofluorescence or flow cytometry.

175 nvolved mitochondrial function; results from immunoblotting, immunofluorescence, and functional assay

176 polymorphism arrays, whole-exome sequencing, immunoblotting, immunofluorescence, electron microscopy,

177 gical and molecular changes were assessed by immunoblotting, immunofluorescence, flow cytometry, real

178 Enzyme assays, immunoblotting, immunohistochemical testing, flow cytome

179 rial function and autophagy were assessed by immunoblotting, immunohistochemistry, and qPCR.

180 on and livers were collected and analyzed by immunoblotting, immunohistochemistry, histology, and rea

181 and mRNA abundance was analyzed by means of immunoblotting, immunohistochemistry, immunofluorescence

182 cells and transfected HEK293T cells through immunoblotting, immunohistochemistry, luciferase activit

183 The effect of infection was examined by immunoblotting, immunoprecipitation, chromatin immunopre

184 out mouse embryonic fibroblasts, we found by immunoblotting, immunoprecipitation, immunostaining, and

185 d leukocytes and by immunohistochemistry and immunoblotting in colon biopsies of patients with active

186 ole of PAK in AD, we first quantified PAK by immunoblotting in homogenates from the parietal neocorte

187 ted repression of GMPS could be validated by immunoblotting in Sk-Hep1, HepG2, and HuH6 cells.

188 Validations were by immunoblotting in systemic and intracoronary blood from

189 Using immunoblotting, in situ zymography, and immunofluorescen

190 Immunoblotting indicated cleavage of caspase-1 and IL-1b

191 igitoxin followed by immunoprecipitation and immunoblotting indicated that DARPP-32 has an important

192 nce that a 2-EIA-based MTTT algorithm, where immunoblotting is replaced by the C6 EIA, performs as we

193 By immunoblotting, kidney cortex homogenate from patients t

194 Immunoblotting measured the size and amount of RPE65 pro

195 Analysis by immunoblotting, metabolic labeling, and mass spectrometr

196 ow cytometry, reverse transcription-PCR, and immunoblotting methodologies do not allow quantitative i

197 Using biochemical and immunoblotting methods, mitochondrial function and phosp

198 ardiomyocytes (CMNCs) and mouse hearts using immunoblotting, molecular imaging, and [(35)S]methionine

199 1 monocytes, protein levels were analyzed by immunoblotting, mRNA levels by real-time polymerase chai

200 sing confocal immunofluorescence microscopy, immunoblotting, myeloperoxidase-DNA complex ELISA, and f

201 tive RT-PCR; protein content was measured by immunoblotting; NOS activity was evaluated with high-per

202 d caspase-3, and extravascular albumin), and immunoblotting (nuclear factor-kappaB, hypoxia-inducible

203 ral lines of evidence from cancer databases, immunoblotting of cancer cells, proliferation, and xenog

204 l evidence in support of dATI is provided by immunoblotting of endogenous truncated proteins enriched

205 microscopy (ESEM) of microstructure, and the immunoblotting of epidermal growth factor (EGF) in EBN.

206 Immunoblotting of isolated glomerular lysates revealed m

207 Immunoblotting of isolated glomerular lysates revealed t

208 Comprehensive quantitative immunoblotting of protein extracts from human embryonic

209 Alpha-gal epitopes were detected by immunoblotting on antivenoms.

210 ckout cardiac myocytes did not show P2X4R by immunoblotting or by any response to the P2X4R-specific

211 for choline/methyl metabolite measurements, immunoblotting or gene expression of relevant enzymes.

212 sphate-ribose) polymerase were quantified by immunoblotting or immunohistochemistry.

213 as analyzed by using phospho flow cytometry, immunoblotting, or co-immunoprecipitation in CD19-defici

214 tected by enzyme-linked immunosorbent assay, immunoblotting, or immunofluorescence.

215 xpression of signaling molecules by means of immunoblotting, PGD2 and macrophage inflammatory protein

216 ce difference gel electrophoresis (2D-DIGE), immunoblotting, quantitative enzyme-linked immunosorbent

217 Tissues were collected for immunoblotting, quantitative polymerase chain reaction,

218 mug/mL), or both (CSE+EtOH), and analyzed by immunoblotting, quantitative reverse-transcription polym

219 Immunoblotting, quantitative RT-PCR, enzymatic assays, n

220 Immunoblotting, radioenzymatic- and fluorescence resonan

221 h quantitative polymerase chain reaction and immunoblotting, respectively.

222 tative polymerase chain reaction and Western immunoblotting, respectively.

223 In all 10 plasma samples immunoblotting revealed activation of factor XII, plasma

224 Immunoblotting revealed downregulation of the medullary

225 In the XLH cultures, immunoblotting revealed more abundant osteopontin (OPN),

226 Immunoblotting revealed rab17 immunoreactive species at

227 icroarray analysis, quantitative RT-PCR, and immunoblotting revealed that IRF2 regulated the basal le

228 DNA image cytometry and immunoblotting revealed that lithium initiated prolifera

229 Deep-sequencing analyses of mRNA and immunoblotting revealed that MYPT1 depletion reduced the

230 Immunoblotting revealed that p38 mitogen-activated prote

231 Immunoblotting revealed that this was associated with ac

232 In genipin-treated TM cells, Western immunoblotting showed a reduction of active MMP2 and MMP

233 fate poly acrylamide gel electrophoresis and immunoblotting showed acid-soluble collagen fraction fro

234 Western immunoblotting showed increases of the iron storage prot

235 N-beta than was the wild-type S. aureus, and immunoblotting showed that IFN-beta interacts with the b

236 Immunoblotting showed that nonsurvivors had increased to

237 Immunoblotting showed that the superior/middle lateral R

238 Western immunoblotting shows enrichment of both isoforms in PSD

239 pathways (showing a dose-dependent effect on immunoblotting), stimulated cellular proliferation (abou

240 Here, with the help of an immunoblotting strategy and Ime4-GFP protein localizatio

241 Targeted LC-MS/MS and immunoblotting studies further confirmed the down regula

242 Moreover, immunoblotting studies of selectively bred and transgeni

243 Immunoblotting suggested that SerpinB2 (cross-linked int

244 sphorylation was detected by two-dimensional immunoblotting, suggesting that loss of SMA-5 function l

245 Immunoblotting suggests that two classes of phosphorylat

246 Further, using semiquantitative immunoblotting techniques, we found that the levels of G

247 6 and LOX-1), was measured using QRT-PCR and immunoblotting techniques.

248 chromatography, and nondenaturing/denaturing immunoblotting techniques.

249 reeze-dried in order to perform SDS-PAGE and immunoblotting tests.

250 First, we confirmed by immunoblotting that BDNF levels are elevated after this

251 e show here by immunoelectron microscopy and immunoblotting that SynCAM 1 is expressed on mouse rod p

252 BUN, creatinine, albuminuria, genotyping and immunoblotting, this APOL1 null individual does not have

253 icroscopy, immunohistochemistry, and Western immunoblotting to examine the morphology and microstruct

254 We applied quantitative immunoblotting to generate profiles of transporters, cha

255 ss-linking followed by mass spectrometry and immunoblotting to isolate and characterize proteins comp

256 human serum IgG4 were identified by means of immunoblotting to screen for potential bacterial allerge

257 We employed affinity purification and immunoblotting to validate the interaction between STAT5

258 Cat r 1 was identified by immunoblotting using allergic patients' sera followed by

259 Immunoblotting using an Ser-235 phosphorylation-specific

260 The presence of ADAM8 in GCF was shown by immunoblotting using anti-human ADAM8 polyclonal antibod

261 n in tobacco (Nicotiana tabacum) leaves, and immunoblotting using Arabidopsis showed localization of

262 th ELISA, size-exclusion chromatography, and immunoblotting using both recombinant proteins and serum

263 a simple but elegant method of quantitative immunoblotting using isoform-specific antibodies and (35

264 Enzyme-linked immunosorbent assays, immunoblotting using monoclonal antibodies and Escherich

265 nd/Sypro-Ruby staining, autoradiography, and immunoblotting using phosphoserine-specific titin-antibo

266 tin modifications were determined by Western immunoblotting using specific antibodies.

267 on SDS-PAGE gels, by direct staining and/or immunoblotting, using antibodies against the tag or the

268 IgE-immunoblotting was also conducted using sera from childr

269 Immunoblotting was employed on tissue from subregions of

270 IgE immunoblotting was performed with pooled sera from food-

271 transcripts by qPCR and protein analysis via immunoblotting was performed.

272 Western immunoblotting was used to detect Fas, FasL, sFasL, and

273 Immunoblotting was used to evaluate SIRT1 expression and

274 the dentate gyrus of anesthetized rats, and immunoblotting was used to measure levels of memory-rela

275 ected to quantitative PCR (qPCR) and Western immunoblotting (WB) for BMP1.

276 Using (32)P-labeled NAD(+) and immunoblotting, we also demonstrate that both subunits o

277 rm of TBCD and using non-denaturing gels and immunoblotting, we analyzed lysates from a number of mou

278 copy in combination with glycosylation shift immunoblotting, we demonstrate that GOAT contains 11 tra

279 andem mass spectrometry analysis followed by immunoblotting, we detected few alterations in levels of

280 y electron microscopy, density gradient, and immunoblotting, we determined that the alphavbeta6 integ

281 In addition, through immunoblotting, we found that AHAs overexpressed the NMD

282 confirmation by pulldown assays followed by immunoblotting, we identified P-selectin and multimerin-

283 Here, using RT-quantitative PCR and/or immunoblotting, we show that expression of mouse Saa3 an

284 says, cell cycle and apoptosis analysis, and immunoblotting were carried out with cell lines or prima

285 extraction, the MNase sensitivity assay, and immunoblotting were used to assess global histone acetyl

286 lectron microscopy, histologic analyses, and immunoblotting were used to determine the effects of ATP

287 Immunofluorescence and immunoblotting were utilized to detect surface-localized

288 easured via direct kinase activity assay and immunoblotting, whereas genes related to mitochondrial b

289 The approach advances microfluidic protein immunoblotting, which is directly relevant to the widely

290 ayed decreased phospho-signal intensities in immunoblotting with anti-phosphoserine/phosphothreonine

291 Accumulation of tagged proteins, detected by immunoblotting with commercial tag antibodies, inversely

292 Sucrose density gradient separation and immunoblotting with known compartment markers were used

293 caspase-9 (CC-9) expression were assayed by immunoblotting with or without SIRT3 enforced expression

294 -polyacrylamide gel electrophoresis SDS-PAGE-immunoblotting with patient sera and rabbit serum anti-P

295 IgE-immunoblotting with peach leaf extract revealed in six p

296 Immunocapture of F0F1-ATPase and immunoblotting with phospho(Ser) PKC substrate antibody

297 ergens were determined and identified by IgE-immunoblotting with pooled sera from prawn-allergic pati

298 Proteins were studied by using SDS-PAGE and immunoblotting with sera from patients with peanut aller

299 s by iTRAQ/LC-MS/MS with TiO2 enrichment and immunoblotting with specific anti-phospho-NHE3 antibodie

300 Immunoblotting with specific antibodies revealed the pre