ライフサイエンスコーパス: importin

1 on of Notch1 (N1IC) through interaction with importins.

2 BP via interactions with 14-3-3 proteins and importins.

3 -3 proteins, CRM-1 (exportin-1 or XPO-1), or importins.

4 ) substantially decreased binding to several Importins.

5 stone tails are recognized by the individual Importins.

6 nzyme UBE2E3 and its nuclear import receptor importin 11 (Imp-11) regulate Nrf2 distribution and acti

7 In this study, we show that Importin-11 (Ipo11) is a transport receptor for PTEN tha

8 nt mice develop lung tumors, also implicates Importin-11 as a novel tumor suppressor.

9 In this issue, Chen et al. show that Importin-11 traffics the tumor suppressor PTEN into the

10 DCD5 at Ser-119 to enhance its stability and importin 13-mediated nuclear translocation of PDCD5.

11 importin-beta, transportin-1, transportin-3, importin-13) or through adaptor proteins (e.g., importin

12 onclassical nuclear import pathway via IPO3 (importin 3, transportin 2) mediates stress-induced NEMO

13 of H3/H4 with the histone chaperone ASF1 and importin 4 is disrupted and the translocation of green f

14 .1-enriched genes and reduced association of Importin 4 with H3.1, but not H3.3.

15 We identified a novel role for importin-4 in governing the nuclear transport of HE4.

16 ermectin, an importin inhibitor, blocked HE4/importin-4 nuclear accumulation and sensitized HE4-overe

17 Here, we identified importin-5 (IPO5), a member of the importin family of nu

18 A myristoylated peptide that blocks importin 7-mediated ERK nuclear translocation induced ro

19 H2O2 plus IL-1beta had no direct effect on importin-7 but caused a significant loss (61.2+/-12.6% c

20 Knockdown of importin-7 by 38.4 +/- 11.5% (compared with control siRN

21 The importin-7 complex is essential for glucocorticoid funct

22 roxide (H2O2) plus IL-1beta costimulation on importin-7 expression, function, and glucocorticoid resp

23 We hypothesized that importin-7 is central to GR nuclear translocation and gl

24 snRNPs also fail to bind Ketel; however, the importin-7 orthologue Moleskin (Msk) physically associat

25 We investigated the effects of importin-7 siRNA on fluticasone propionate (FP)-induced

26 of Skp2, disruption of Skp2 interaction with importin-7, and decreased levels of p27/p57 in mouse len

27 of nuclear RanGTP, an essential cofactor for importin-7-mediated nuclear import of cargo proteins.

28 dentified TATA-Box Binding Protein (TBP) and Importin 8 (IPO8) to be stable in non-small cell lung ca

29 We have identified importin 8 as a factor that directly imports eIF4E into

30 We found that importin 8 is highly elevated in untreated patients with

31 Importin 8 only imports cap-free eIF4E.

32 ical step in its regulation and position the importin 8-eIF4E complex as a novel therapeutic target.

33 Importantly, we identified importin 9 as the nuclear import factor for actin.

34 ctive maintenance of nuclear actin levels by importin 9 is required for maximal transcriptional activ

35 r Formin-2 or actin's nuclear import factor, importin-9, increases the number of DNA double-strand br

36 RNAi screening of both importin alpha and beta family members, as well as co-im

37 only the open-form is capable of binding to importin alpha and that, upon binding, the 627 domain sa

38 ition with DHBc for the host kinase, whereas importin alpha binding by CTD may contribute to inhibiti

39 Atomic structures of importin alpha bound to two variants of NLS2 revealed NL

40 Sequestration of importin alpha by GM130 liberates the spindle assembly f

41 tion of ChREBP.14-3-3 and inhibit the ChREBP/importin alpha interaction, resulting in cytosolic local

42 importin alpha1 and discuss the evolution of importin alpha isoforms.

43 he nuclear import factor Srp1 (also known as importin alpha or karyopherin alpha) is required for ubi

44 mportin alpha, and activated in stage 8 when importin alpha partitions to a membrane pool.

45 on between AtREM1.3 and four isoforms of the importin alpha protein family in a yeast two-hybrid syst

46 rained proteomimetics to target the HNF1beta-importin alpha protein-protein interaction were designed

47 decreases the interaction strength with the importin alpha proteins.

48 The human genome encodes six isoforms of importin alpha that show greater than 60% sequence simil

49 al nuclear localization signal that recruits importin alpha to the Golgi membranes.

50 ironment that facilitates bringing Nup50 and importin alpha together, as well as other soluble factor

51 he Golgi matrix protein GM130 interacts with importin alpha via a classical nuclear localization sign

52 ethylated H3 lysine 9 [H3K9me3]), and DIM-3 (importin alpha), which is involved in DIM-5 localization

53 n stage 3 spindles by the transport receptor importin alpha, and activated in stage 8 when importin a

54 try and repressor function, independently of importin alpha, its classical partner.

55 However, Nup50 dynamics are independent of importin alpha, Nup153, and Nup98, even though the latte

56 orresponding to NLS1 and NLS2 bind weakly to importin alpha, the two NLSs synergize in the context of

57 rimarily binds the major-NLS binding site of importin alpha, unlike NLS1 that associates with the min

58 red for efficient association between NP and importin alpha, which is crucial for IAV RNP nuclear tra

59 terminal half of the protein, which contains importin alpha- and Nup153-binding domains.

60 itotic NPC segregation is independent of its importin alpha- and Ran-binding domains but relies on a

61 facilitated by the nucleoporin Nup2 via its importin alpha- and Ran-binding domains.

62 phages, NLS peptide specifically blocked the importin alpha-mediated nuclear import of NF-kappaB and

63 librium in the vicinity of the C-terminus of importin alpha.

64 he distal tail of Nup153 and is dependent on importin alpha.

65 XCKT2 MT crosslinking activity by releasing importin alpha/beta from a bipartite nuclear localizatio

66 Although a classical NLS and importin alpha/beta mediated nuclear import pathway has

67 e actively trafficked to the nucleus via the importin alpha/beta pathway.

68 ed with fluorescent cargos (mCherry) for the importin alpha/beta, transportin 1, and transportin 3 im

69 type ICP27 protein inhibited both classical, importin alpha/beta-dependent and transportin-dependent

70 not with a mutant XCTK2 that cannot bind to importin alpha/beta.

71 Coexpression of importin-alpha and inhibition of nuclear export by lepto

72 0 binds to PKCdelta with similar kinetics as importin-alpha and is required for the interaction of im

73 imatinib both blocked binding of PKCdelta to importin-alpha and nuclear import, demonstrating that ty

74 Cargo proteins interact with the importin-alpha armadillo repeat domain via nuclear local

75 mRPs and identified a repeat pair from yeast importin-alpha as having the optimal curvature geometry

76 Comparison of the importin-alpha binding affinities of HaRxL106 and other

77 s of ChREBP with alanine resulted in loss of importin-alpha binding, glucose-stimulated transcription

78 d dimers adopted a conformation incapable of importin-alpha binding.

79 tent with yeast and mammalian proteins, rice importin-alpha binds the prototypical NLS from simian vi

80 nuclear transport receptors belonging to the importin-alpha family in nucleolar accumulation of the P

81 Importin-alpha has two separate NLS binding sites (the m

82 binding assays, we show that it is bound by importin-alpha in almost the same manner and with simila

83 PMTV TGB1 interacted with importin-alpha in N. benthamiana, which was detected by

84 t and the amino-terminal domain required for importin-alpha interaction in plants, nucleolar targetin

85 siRNA knockdown of the human importin-alpha isoform KPNA2, corresponding to the murin

86 a isoform KPNA2, corresponding to the murine importin-alpha isoform previously shown to bind to DENV-

87 on studies using DENV-2 and -4 NS5 and human importin-alpha isoforms failed to identify an interactio

88 t mice, to understand the role of individual importin-alpha isoforms in adaptation.

89 n (IFN) response by hijacking select nuclear importin-alpha isoforms.

90 os are preferentially imported by a distinct importin-alpha it remains unknown how this specificity i

91 However, owing to the lack of importin-alpha knockout animal models in the past, their

92 portin-alpha proteins but interact with rice importin-alpha more strongly.

93 Importin-alpha mutants that impair interactions during n

94 the characteristic nuclear import defect of importin-alpha mutants, whereas srp1-49 shows a defect i

95 or importin-alpha, sequence variation at the importin-alpha NLS-binding sites and tissue-specific exp

96 iated by the importin-beta binding domain of importin-alpha operates in plants, with NLS-mimicking se

97 ural armadillo repeat proteins (nArmRP) like importin-alpha or beta-catenin bind their target peptide

98 etermine redundant and specific functions of importin-alpha paralogs from Arabidopsis thaliana.

99 Virus-induced gene silencing of two importin-alpha paralogs in Nicotiana benthamiana resulte

100 Plant genomes typically encode several importin-alpha paralogs that can have both specific and

101 functional with plant, mammalian, and yeast importin-alpha proteins but interact with rice importin-

102 of their complexes with mouse (Mus musculus) importin-alpha show preferential binding to the major NL

103 stal structures of their complexes with rice importin-alpha show that they bind to the minor NLS bind

104 However, the part of the tryptophan array of importin-alpha that is essential for the recognition of

105 importin-alphas determine formation of cargo/importin-alpha transport complexes in plant cells.

106 alpha and is required for the interaction of importin-alpha with the NLS.

107 Srp1 (importin-alpha) can translocate proteins that contain a

108 The C-terminal region (which binds importin-alpha) inhibits mitotic spindle pole formation.

109 alization signals, recognized by the adaptor importin-alpha, and the PY nuclear localization signals,

110 Our results suggest that cargo affinity for importin-alpha, sequence variation at the importin-alpha

111 ortin-13) or through adaptor proteins (e.g., importin-alpha, snurportin-1, symportin-1), as well as u

112 iruses, in importin-alpha-silenced cells and importin-alpha-knockout mice, to understand the role of

113 raction is needed for cell-to-cell movement, importin-alpha-mediated nucleolar targeting of TGB1 is a

114 of avian and mammalian influenza viruses, in importin-alpha-silenced cells and importin-alpha-knockou

115 oteins are recognized by the import receptor importin-alpha.

116 interaction with the nuclear import protein importin-alpha.

117 in a conformation that precludes binding of importin-alpha.

118 Here, we delineate the choreography of importin-alpha/CAS complex assembly and disassembly in p

119 nation of protein import, and disassembly of importin-alpha/CAS complexes after export occurs in the

120 the coordinated assembly and disassembly of importin-alpha/CAS complexes for generating productive t

121 TPase-activating factors to demonstrate that importin-alpha/CAS complexes form in the nuclear basket

122 lysine/arginine residues, unlike other known importin-alpha:beta-dependent NLSs.

123 311/321: EEPPAKRQCIE) that is recognized by importin-alpha:beta.

124 Binding of this domain to Importin alpha1 (Impalpha1) was confirmed by gel filtrat

125 romolar Kd, that is selective for the murine Importin alpha1 (mImpalpha1) minor site, with the Kd str

126 y of RCC1 for importin alpha3 vs the generic importin alpha1 and discuss the evolution of importin al

127 Importin alpha1 can bind classical nuclear localization

128 f magnitude higher affinity than the generic importin alpha1, although the two isoforms share an iden

129 CAS, exportin-2) and its transport substrate importin-alpha1 (imp-alpha1) among significantly up-regu

130 d we also analyzed transcriptional levels of importins-alpha1, -alpha2, and -beta2 and different expo

131 the crystal structure of rice (Oryza sativa) importin-alpha1a at 2-A resolution.

132 c1 GTPase activity and is associated with an importin-alpha2 redistribution.

133 nd Rel-A, are translocated to the nucleus by importin alpha3 and importin alpha4.

134 We propose importin alpha3 evolved to recognize topologically compl

135 mains or are masked by quaternary structures.Importin alpha3 facilitates the nuclear transport of the

136 Here we identify the mechanisms of importin alpha3 selectivity for RCC1.

137 Importin alpha3 uses its greater conformational flexibil

138 ecular basis for the selectivity of RCC1 for importin alpha3 vs the generic importin alpha1 and discu

139 and beta-propeller, disrupts specificity for importin alpha3, demonstrating the structural context ra

140 t of Ran exchange factor RCC1 is mediated by importin alpha3.

141 The expression of importin-alpha3 and Rel-A in the lung of OVA-sensitized

142 reduced blood Tregs, increased expression of importin-alpha3 and Rel-A in the lung tissue.

143 Overexpression of miR-181b inhibited importin-alpha3 expression and an enriched set of NF-kap

144 ing in the vascular endothelium by targeting importin-alpha3, a protein that is required for nuclear

145 ole in controlling inflammation by targeting importin-alpha3, a regulator of NF-kappaB nuclear import

146 A crystal structure of the importin-alpha3/MOS6 armadillo repeat domain suggests th

147 ocated to the nucleus by importin alpha3 and importin alpha4.

148 Further investigation indicated that, importin-alpha4 and importin-alpha7 directly interacted

149 ggest that upon DNA damage transport adaptor importin-alpha4 imports XPA into the nucleus in an ATR-d

150 he nuclear localization signal (NLS) of XPA, importin-alpha4 or/and importin-alpha7 are required for

151 Interestingly, the binding of importin-alpha4 to XPA was dependent on UV-irradiation,

152 R reduced the amount of XPA interacting with importin-alpha4.

153 in Kd obtains in binding studies with human Importin alpha5 (hImpalpha5), which in some cases has be

154 miR181b-3p modulates expression of importin alpha5 and sensitivity of TLR4-mediated signali

155 This study identifies a miR181b-3p-importin alpha5 axis in regulating inflammatory signalin

156 the nuclear localization signal of ACSS2 for importin alpha5 binding and nuclear translocation.

157 ortin alpha5 or to a preassembled complex of importin alpha5 bound to the C-terminal domain of the im

158 e to actively displace the import cargo from importin alpha5 but rather to prevent cargo rebinding in

159 The isoform importin alpha5 can bind phosphorylated cargos such as S

160 Overexpression of miR181b-3p decreased importin alpha5 expression and normalized lipopolysaccha

161 181b-3p in liver and increased expression of importin alpha5 in nonparenchymal cells.

162 ast, PB2 can only bind with high affinity to importin alpha5 in the absence of Nup50.

163 residues of Nup50 bind to the C terminus of importin alpha5 like a "clip," stabilizing the closed co

164 p50 binds with high affinity either to empty importin alpha5 or to a preassembled complex of importin

165 s, was identified as a target of miR181b-3p; importin alpha5 protein was increased in Kupffer cells f

166 Importin alpha5, a protein involved in p65 translocation

167 he interaction of the nucleoporin Nup50 with importin alpha5.

168 transactivator of the nuclear import factor importin-alpha5 (Impalpha5), and interfering with this m

169 on of karyopherin-alpha1 (KPNA1, also called importin-alpha5), which is known to mediate the nuclear

170 NLS in trans, which ensures high avidity for importin alpha7 while preventing non-specific binding to

171 he full length NP to confer high avidity for importin alpha7, explaining why the virus efficiently re

172 signal (NLS) of XPA, importin-alpha4 or/and importin-alpha7 are required for the XPA nuclear import.

173 tigation indicated that, importin-alpha4 and importin-alpha7 directly interacted with XPA in cells.

174 portin-alpha7 was not, suggesting a role for importin-alpha7 in nuclear translocation of XPA in the a

175 Here, we demonstrate that importin-alpha7 is involved in the formation of EBOV inc

176 However, deletion of the gene encoding importin-alpha7 was not sufficient to increase survival

177 dent on UV-irradiation, while the binding of importin-alpha7 was not, suggesting a role for importin-

178 Importin-alphas are essential adapter proteins that recr

179 Importin-alphas behaved similarly to 5S rRNA.

180 tes and tissue-specific expression levels of importin-alphas determine formation of cargo/importin-al

181 in suggests that five of the six Arabidopsis importin-alphas expressed in rosette leaves have an almo

182 nto the nucleus by a pathway that depends on importin and chromatin-modifying protein 1.

183 f macromolecules through the nuclear pore by importins and exportins plays a critical role in the spa

184 ins of the karyopherin superfamily including importins and exportins represent an essential part of t

185 rosophila and discovered a critical role for importins and exportins, Ran-GTP cycle regulators, nucle

186 s of the karyopherin-beta superfamily termed importins and exportins.

187 om the ER membrane, making it accessible for importins and nuclear import.

188 ion of the Hxk2 NLS1 motif by Kap60 and both importins are essential for Hxk2 nuclear import.

189 place Klf6 at the nexus of a novel gp130-Klf-importin axis, which promotes differentiation and viabil

190 We conclude that the cell contains importin beta and transportin "global positioning system

191 The nuclear import receptors importin beta and transportin play a different role in m

192 Here, we report that KPNB1, an importin beta component of the ncRNA repressor of nuclea

193 e a mechanism of EGFR regulation through the importin beta family member RAN-binding protein 6 (RanBP

194 ation analyses, revealed that a nonclassical importin beta family member, IPO3, was the only importin

195 GTP produced on chromatin frees factors from importin beta for localized assembly.

196 cible inhibition of the conserved Drosophila importin beta in lateral neurons abolishes behavioral rh

197 vitro reconstitution assays demonstrate that importin beta is critically required for ubiquitination

198 Importin beta is known to act by repressing assembly fac

199 monstrate that the Sel1L, Hrd1, p97/VCP, and importin beta proteins are required for the dislocation

200 -regulating release of assembly factors from importin beta, and 2) direct action by transportin bindi

201 nuclear transport receptors (NTRs), NTF2 and Importin beta, together with the concomitant film thickn

202 nd exportin 1 and involved the activation of importin beta-dependent nuclear import of 53BP1, a large

203 of JMY requires both the WH2/NLS region and importin beta.

204 Soluble karyopherins of the importin-beta (impbeta) family use RanGTP to transport c

205 ta-arrestins (Arrbs), the small GTPase Rab5, importin-beta (Kpnb1), and transportin-1 (Tnpo1).

206 ur results suggest that rAAV2 interacts with importin-beta alone or in complex with other karyopherin

207 signal (NLS) at its C-terminus that binds to importin-beta and is required for cortical polarity and

208 psid proteins from rAAV2 could interact with importin-beta and that this interaction was sensitive to

209 Of interest, the importin-beta binding (IBB) domain of SPN1, which is ess

210 the autoinhibitory mechanism mediated by the importin-beta binding domain of importin-alpha operates

211 5 nuclear localization, whereas knockdown of importin-beta did.

212 These data indicate that Ketel/importin-beta does not play a significant role in Drosop

213 extended our studies to other members of the importin-beta family and found that all tested karyopher

214 The importin-beta family members (karyopherins) mediate the

215 Msn5 and Los1, members of the importin-beta family, function in tRNA nuclear export.

216 Whereas full-length AR requires Hsp90 and importin-beta for active nuclear translocation, basal nu

217 of MKL1, which affects its interaction with Importin-beta for efficient nuclear import.

218 tazole, a small-molecule inhibitor of RanGTP/importin-beta function, to study the role of Ran in spin

219 These results reveal previously unrecognized importin-beta functions at kinetochores exerted via RANB

220 3, two integral nuclear pore components, and importin-beta IMB-1 provides strong evidence that this r

221 mics simulations using crystal structures of Importin-beta in its free form or in complex with nuclea

222 other serotypes and found that the extent of importin-beta interaction varied, suggesting that differ

223 Importin-beta interacts through this region with NUP358/

224 Importin-beta is the main vector for interphase nuclear

225 , small interfering RNA (siRNA) knockdown of importin-beta partially inhibited rAAV2 nuclear transloc

226 Here we show that importin-beta regulates multiple aspects of mitosis via

227 describe how microtubules and the RAN GTPase/importin-beta system collaborate to control timing of HU

228 Overexpression of importin-beta, or of the nucleoporin-binding region, inh

229 icated that vertebrate snRNP import requires importin-beta, the transport receptor that binds directl

230 family, which can bind cargo directly (e.g., importin-beta, transportin-1, transportin-3, importin-13

231 d particles revealed that rAAV2 localized to importin-beta-dense regions of cells in late trafficking

232 restored RANGAP1 to kinetochores and rescued importin-beta-dependent mitotic dynamic defects.

233 Co-expressing either importin-beta-interacting RANBP2 fragments, or CRM1, res

234 r experiments using dual-fluorophore-labeled Importin-beta.

235 capsid severely inhibited interactions with importin-beta.

236 la SNUP (dSNUP) does not interact with Ketel/importin-beta.

237 he complex between the Ras homologue Ran and Importin-beta.

238 eta-catenin to a greater extent than that of importin-beta.

239 ormations can be simultaneously regulated by Importin beta1 (a major transport receptor) and RanGTP (

240 ulators of Epac1 activity, we show here that importin beta1 (impbeta1) is an Epac1 binding partner th

241 an interaction of the C9-isoforms with both Importin beta1 and Ran-GTPase, components of the nuclear

242 ta-actin, GAP-43, Neuritin, Reg3a, Hamp, and Importin beta1 mRNAs.

243 formed cells, and the translocation required importin beta1, nucleolin, and Smad2/3.

244 Nuclear import of ErbB3 occurs via importin beta1, which drives the receptor through the nu

245 ta-catenin cytoplasmic stabilization and the importin beta1/Ran-mediated transport system.

246 We show that although importin beta2 binding does not regulate anillin's funct

247 w that anillin is targeted to the nucleus by importin beta2 in a Ran-dependent manner through an atyp

248 Depletion of endogenous importin-beta5 associated with IQGAP1 also reduced expre

249 be a regulator of beta-catenin function via importin-beta5.

250 Importin binding assays indicate that nuclear access occ

251 large-scale conformational changes prior to importin binding.

252 dle self-organization through the release of importin-bound spindle assembly factors (SAFs), which st

253 y of nuclear import pathways are mediated by importin-cargo interactions.

254 cellular dynamics, we developed in vivo beta-importin complex coimmunoprecipitation (co-IP) assays us

255 e mediated by direct binding of TRIM3 to the Importin complex.

256 calisation signal)-cargo release from RanGTP-importin complexes.

257 In particular, we provide evidence for importin-dependent long-distance transport from synapto-

258 he perinuclear area and at the spindle in an Importin-dependent manner during cell cycle progression.

259 s revealed that Tsr2 efficiently dissociates importin:eS26 complexes via an atypical RanGTP-independe

260 lso used in fluorescence assays to find that importins facilitate the transport of signal-tagged albu

261 The evolutionarily conserved beta-importin family member Los1 (Exportin-t) has been the on

262 t in vivo biochemical evidence that two beta-importin family members, Los1 (exportin-t) and Msn5 (exp

263 dentified importin-5 (IPO5), a member of the importin family of nuclear transport proteins, as an int

264 he histone chaperone Asf1b are important for Importin-histone recognition.

265 ition of VEEV capsid protein (C) by the host importin (IMP) alpha/beta1 nuclear transport proteins.

266 oteomics approach to identify members of the importin (IMP) family of nuclear transporters as interac

267 d nucleoporins Nup153 or Nup214 or some beta importins (Imp7 or Impbeta), it mediates the association

268 studies of H3 and H4 tails binding to seven Importins, Impbeta, Kapbeta2, Imp4, Imp5, Imp7, Imp9, an

269 s indicate that nuclear access occurs via an importin-independent mechanism.

270 Here, we identify a code that determines importin-independent nuclear import of ankyrin repeats (

271 DAR (RanGDP/AR) pathway represents a general importin-independent nuclear import pathway and is frequ

272 ecessitating the identification of a general importin-independent nuclear import pathway.

273 Treatment with ivermectin, an importin inhibitor, blocked HE4/importin-4 nuclear accum

274 The nuclear importin IPO5 was identified as a novel interacting prot

275 is an import substrate of the carriers alpha-importin (Kap60 in yeast) and beta-importin (Kap95 in ye

276 ers alpha-importin (Kap60 in yeast) and beta-importin (Kap95 in yeast).

277 hosphorylation, and its interaction with the importin karyopherin alpha2.

278 PY-NLS is also capable of import through the importin/karyopherin beta1 pathway but was not functiona

279 expression levels of an NF-kappaB-associated importin (KPNA4: one of the proteins responsible for the

280 which is transported to the cell body in an importin-mediated manner.

281 7 nm, respectively, and binds the other five Importins more weakly.

282 In contrast, beta-importin Msn5 preferentially assembles with RanGTP and s

283 Yet not all nuclear proteins interact with importins, necessitating the identification of a general

284 ng elements overlap with the binding site of importins on TPX2.

285 nopore by nuclear transport receptors (e.g., importins) owing to their interactions with barrier-form

286 overs Hxk2 as a new cargo for the alpha/beta-importin pathway of S. cerevisiae.

287 capacity to interact with the same subset of importin proteins.

288 eins were chaperones, cytoskeletal proteins, importins, proteins involved in ubiquitination, kinases

289 Importin receptors are associated with nuclear transloca

290 lecular basis of the affinity of 627-NLS for importins remained unclear from these structures, appare

291 r localization sequence is a determinant for importin specificity.

292 ment of cells with 6 and 22 also upregulated importin subunit alpha-2 levels and repressed translatio

293 ortin beta family member, IPO3, was the only importin that was able to associate with NEMO and whose

294 ed transport receptors, called exportins and importins, that interact with cargo proteins in a RanGTP

295 lexibility therefore enables 627-NLS to bind importin through conformational selection from a tempera

296 3 and H4 are known to bind several different Importins to import the histones into the cell nucleus.

297 rs bound to the WH2 domains block binding of importins to the NLS and prevent nuclear import of JMY.

298 nding protein (CREB), and by an inhibitor of importin, which is required for activated CREB to get in

299 Our model is that during anaphase, "free" importins, whose gradient inversely correlates with acti

300 r two basic segments to bind the same set of Importins with a similar trend of relative affinities as