ライフサイエンスコーパス: importin-alpha

1 res dephosphorylated ChREBP to interact with importin alpha.

2 classical NLS (cNLS), which is recognized by importin alpha.

3 0 NLS is predicted to remain free to bind to importin alpha.

4 MUC1-C associates with importin beta and not importin alpha.

5 IE2 as well as the interaction of UL84 with importin alpha.

6 n of VeA to the nucleus was dependent on the importin alpha.

7 with the classic nuclear transport receptor, importin alpha.

8 ecreases the binding affinity of the NLS for importin alpha.

9 ng proteins is mediated by the cNLS receptor importin alpha.

10 phorylation-dependent change in affinity for importin alpha.

11 -157 interrupted the association of p27 with importin alpha.

12 and binds directly and with high affinity to importin alpha.

13 and the armadillo repeat core of vertebrate importin alpha.

14 hat presents a distinct motif for binding to importin alpha.

15 ine phosphatase rescued its association with importin alpha.

16 S-cargo binding to the NLS binding pocket on importin alpha.

17 beta, which binds to cargoes via the adaptor importin alpha.

18 , the importin beta-like export receptor for importin alpha.

19 librium in the vicinity of the C-terminus of importin alpha.

20 he distal tail of Nup153 and is dependent on importin alpha.

21 yopherin beta2, importin 4, importin 11, and importin alpha.

22 and exclusively to the minor binding site of importin alpha.

23 at allows exposure of the NLS and binding of importin-alpha.

24 pha transport receptor and directly binds to importin-alpha.

25 and in response to interactions with Ran and importin-alpha.

26 al-3, but not mutant Gal-3 (R224A), binds to importin-alpha.

27 n autoregulatory function similar to that of importin-alpha.

28 mplex shows that Nup50 binds at two sites on importin-alpha.

29 o interact with the nuclear adaptor protein, importin-alpha.

30 s well as efficient nuclear export of Kap60p/importin-alpha.

31 oteins are recognized by the import receptor importin-alpha.

32 interaction with the nuclear import protein importin-alpha.

33 in a conformation that precludes binding of importin-alpha.

34 entify an in vivo ADAR3 interaction partner, importin alpha 1 (KPNA2) that specifically recognizes an

35 embrane integration and remain proximal with importin-alpha-16 after integration into the endoplasmic

36 Thus, the association of importin-alpha-16 and INM-directed proteins appears to r

37 pathway of INM-directed proteins mediated by importin-alpha-16 are highly conserved, and mammalian KP

38 Both LBR and nurim cross-link with Sf9 importin-alpha-16 during cotranslational membrane integr

39 Spodoptera frugiperda (Sf9) importin-alpha-16 is a translocon-associated protein tha

40 K, E26, and the cellular INM-sorting protein importin-alpha-16 is not static; rather, these sorting p

41 n analysis using an ODV envelope protein and importin-alpha-16 shows that during viral infection, imp

42 -alpha-16 shows that during viral infection, importin-alpha-16 translocates across the pore membrane

43 lian INM proteins, cross-linked complexes of importin-alpha-16 with human lamin B receptor (LBR) and

44 Whereas wild type 12 S E1A binds importin-alpha 3, binding affinity was markedly reduced

45 sites within its RRMs, PABPC interacts with importin alpha, a component of the classical import path

46 ct an interaction between HTNV N protein and importin alpha, a nuclear import molecule responsible fo

47 quires adaptation of the viral polymerase to importin-alpha, a cellular protein that mediates transpo

48 binds cytoplasmic cargo, most often via the importin-alpha adaptor, and then transports it through n

49 Importin alpha also contributes to nuclear size changes

50 These results suggest that interactions with importin alpha and 14-3-3 regulate movement of ChREBP in

51 this binding is inhibited in the presence of importin alpha and beta (alpha/beta) and restored by add

52 RNAi screening of both importin alpha and beta family members, as well as co-im

53 f RCC1 is required to prevent the binding of importin alpha and beta to RCC1, thereby allowing RCC1 t

54 nuclear import despite its interaction with importin alpha and beta.

55 We propose that the N-terminal IBB domain of importin alpha and Cse1p function together in NLS-cargo

56 ligand-induced association of CRABP-II with importin alpha and is critical for nuclear localization

57 pha in brain lysates, and the interaction of importin alpha and NR1 in neurons is modulated by PKC ac

58 the concentrations of two transport factors, importin alpha and Ntf2, was sufficient to account for n

59 overlap between the N-terminal IBB domain of importin alpha and other factors implicated in NLS-cargo

60 a-induced nitrosylation and association with importin alpha and Ran and ablates CLIC4 nuclear translo

61 on of CLIC4 with the nuclear import proteins importin alpha and Ran.

62 Adaptors such as importin alpha and snurportin associate with importin be

63 ransported into the nucleus by more than one importin alpha and suggest that E1 phosphorylation by ho

64 only the open-form is capable of binding to importin alpha and that, upon binding, the 627 domain sa

65 to act through its nuclear import receptors importin alpha and/or importin beta to control the seque

66 ults suggest an important mechanism by which importin-alpha and 14-3-3 control movement of ChREBP in

67 Importin-alpha and 14-3-3 were found to bind competitive

68 ) required for the in vitro interaction with importin-alpha and for nuclear import of full-length Sgk

69 Coexpression of importin-alpha and inhibition of nuclear export by lepto

70 0 binds to PKCdelta with similar kinetics as importin-alpha and is required for the interaction of im

71 imatinib both blocked binding of PKCdelta to importin-alpha and nuclear import, demonstrating that ty

72 components: the known CBC subunits CBP20 and importin-alpha and three novel proteins that are only pr

73 the interaction between Gal-3 and importins (importins alpha and beta) that carry the NLS harboring n

74 necessary for nuclear entry, but not Kap60 (importin-alpha), and exportin Msn5 was required for nucl

75 n stage 3 spindles by the transport receptor importin alpha, and activated in stage 8 when importin a

76 omplex containing the major egg lamin, XLB3, importin alpha, and importin beta.

77 finity of an NLS cargo for the NLS receptor, importin alpha, and the import rate for this cargo.

78 affinity of an NLS for the import receptor, importin alpha, and the steady-state accumulation of the

79 lo repeat with high structural similarity to importin-alpha, and the Cdc48-binding site could be mapp

80 alization signals, recognized by the adaptor importin-alpha, and the PY nuclear localization signals,

81 terminal half of the protein, which contains importin alpha- and Nup153-binding domains.

82 itotic NPC segregation is independent of its importin alpha- and Ran-binding domains but relies on a

83 facilitated by the nucleoporin Nup2 via its importin alpha- and Ran-binding domains.

84 Importin alphas are import receptors for nuclear localiz

85 Importin-alphas are essential adapter proteins that recr

86 Cargo proteins interact with the importin-alpha armadillo repeat domain via nuclear local

87 mRPs and identified a repeat pair from yeast importin-alpha as having the optimal curvature geometry

88 ires the NLS-dependent recognition of Sgk by importin-alpha as well as the PI3-kinase-dependent phosp

89 Most animal importin alphas assort into alpha1, alpha2, and alpha3 g

90 Importin-alphas behaved similarly to 5S rRNA.

91 This interaction is activity dependent, with importin alpha being released following NMDA receptor ac

92 response to pro-oxidant stimuli and that the importin alpha-beta heterodimer nuclear import receptor

93 Previously, importin alpha/beta and RanGTP were shown to act as duel

94 Our data show that importin alpha/beta directly regulates the activity of X

95 XCKT2 MT crosslinking activity by releasing importin alpha/beta from a bipartite nuclear localizatio

96 ifically transported into the nucleus by the importin alpha/beta import pathway, and binds directly a

97 to uncover additional downstream targets of importin alpha/beta in mitosis.

98 spindle assembly factors from inhibition by importin alpha/beta in the vicinity of the chromosomes.

99 Although a classical NLS and importin alpha/beta mediated nuclear import pathway has

100 s between Npa3 and proteins in the classical importin alpha/beta pathway for nuclear import.

101 e actively trafficked to the nucleus via the importin alpha/beta pathway.

102 egulates the association of Kinesin-14s with importin alpha/beta to prevent aberrant cross-linking an

103 ed with fluorescent cargos (mCherry) for the importin alpha/beta, transportin 1, and transportin 3 im

104 of a bipartite NLS in the tail of XCTK2 with importin alpha/beta, which regulates its ability to cros

105 type ICP27 protein inhibited both classical, importin alpha/beta-dependent and transportin-dependent

106 hexon to the nuclear import machinery via an importin alpha/beta-dependent mechanism.

107 ocess, because mutation of the NLS abolishes importin alpha/beta-mediated regulation of XCTK2 microtu

108 eceptor CRM1 and the nuclear import receptor importin alpha/beta.

109 associates with the nuclear import receptor importin alpha/beta.

110 endent process that requires the heterodimer importin alpha/beta.

111 not with a mutant XCTK2 that cannot bind to importin alpha/beta.

112 e nuclear entry of NC involves the classical importin-alpha/beta (Kap60p/95p) pathway.

113 ranslocated to the nucleus, in part, via the importin-alpha/beta route and that Arg(224) amino acid r

114 lthough LKB1 is imported into the nucleus by importin-alpha/beta, STRADalpha and MO25 passively diffu

115 t nuclear import of LEDGF/p75 is GTP-, Ran-, importin-alpha/beta-, and energy-dependent and that the

116 Ran-GTPase, and the dimeric import receptor, importin-alpha/beta.

117 he nucleus through the classic karyopherins (importins-alpha/beta) pathway.

118 ocalization signals (NLS) is mediated by the importin-alpha:beta complex that binds cargo in the cyto

119 hrough the nuclear pore complex (NPC) by the importin-alpha:beta receptor.

120 lysine/arginine residues, unlike other known importin-alpha:beta-dependent NLSs.

121 is a Ran binding protein and a cofactor for importin-alpha:beta-mediated import.

122 pore complexes and is required for efficient importin-alpha:beta-mediated nuclear protein import as w

123 311/321: EEPPAKRQCIE) that is recognized by importin-alpha:beta.

124 Importin alpha binding and nuclear import of myopodin ar

125 We show that importin alpha binding and the subsequent nuclear import

126 ition with DHBc for the host kinase, whereas importin alpha binding by CTD may contribute to inhibiti

127 erminal nuclear localization signal abrogate importin alpha binding.

128 Comparison of the importin-alpha binding affinities of HaRxL106 and other

129 In this work, we recharacterized the importin-alpha binding nuclear localization signal (NLS)

130 the conserved prolines to alanines enhanced importin-alpha binding to PKCdelta and induced its nucle

131 s of ChREBP with alanine resulted in loss of importin-alpha binding, glucose-stimulated transcription

132 d dimers adopted a conformation incapable of importin-alpha binding.

133 We show that importin alpha binds to a nuclear localization signal (N

134 tent with yeast and mammalian proteins, rice importin-alpha binds the prototypical NLS from simian vi

135 Interestingly, importin 7, importin 8, and importin alpha bound GR even in the absence of hormone;

136 Atomic structures of importin alpha bound to two variants of NLS2 revealed NL

137 PLSCR1 NLS binds to armadillo repeats 1-4 of importin alpha, but its interaction partially overlaps t

138 Sequestration of importin alpha by GM130 liberates the spindle assembly f

139 of the importin beta-binding (IBB) domain of importin alpha by importin beta is critical for the nucl

140 g site, whereas the second extends along the importin-alpha C-terminus.

141 Srp1 (importin-alpha) can translocate proteins that contain a

142 Here, we delineate the choreography of importin-alpha/CAS complex assembly and disassembly in p

143 nation of protein import, and disassembly of importin-alpha/CAS complexes after export occurs in the

144 the coordinated assembly and disassembly of importin-alpha/CAS complexes for generating productive t

145 TPase-activating factors to demonstrate that importin-alpha/CAS complexes form in the nuclear basket

146 This decrease in cargo affinity for importin alpha correlates with a decrease in nuclear acc

147 , indicating that lamin assembly is Ran- and importin alpha-dependent in the egg extract.

148 In this manner, we demonstrate importin-alpha-dependent nuclear import of Nuk6 and Nuk7

149 ells, we have developed a method that allows importin-alpha-dependent nuclear import to be assayed in

150 tes and tissue-specific expression levels of importin-alphas determine formation of cargo/importin-al

151 Nup50 is then displaced on formation of the importin-alpha export complex.

152 in suggests that five of the six Arabidopsis importin-alphas expressed in rosette leaves have an almo

153 the nine members of the Arabidopsis thaliana importin alpha family in Agrobacterium transformation.

154 nuclear transport receptors belonging to the importin-alpha family in nucleolar accumulation of the P

155 Like other importin-alpha family members, Imp1p supports nuclear pr

156 Notably, 14-3-3 appears to compete with importin alpha for ChREBP binding.

157 ha inhibits import of LKB1 by competing with importin-alpha for binding to LKB1.

158 y sequence for the NLS binding pocket impact importin alpha function in vivo.

159 s elegans, and mouse suggest that the animal importin alpha gene family evolved from ancestral plant-

160 ious path for the evolution of the mammalian importin alpha gene family from an ancestral alpha1-like

161 The conventional importin alpha gene family in metazoan animals is compos

162 n alphas provide insight into how the animal importin alpha gene family may have evolved from the mos

163 we extended the phylogenetic analysis of the importin alpha gene family to nonbilateral animals and i

164 onal basis for a unified nomenclature of the importin alpha gene family.

165 In budding yeast there is a single importin-alpha gene and in higher eukaryotes there are m

166 We cloned the importin-alpha gene from T. gondii (TgIMPalpha), which e

167 l expansion and specialization of the animal importin alpha-gene family may have been driven by the s

168 rk for studies of the more complex mammalian importin alpha-gene family.

169 ikelihood analysis suggests that animal-like importin alpha genes occur in the Choanoflaggelate Monos

170 Importin-alpha has two separate NLS binding sites (the m

171 Functional homologs of importin-alpha have been characterized in a wide range o

172 the differential cargo selectivity of plant importin-alpha homologs has not been established.

173 g (VIGS) to knock down the expression of two importin-alpha homologs, NbImpalpha1 and NbImpalpha2, wh

174 The P protein did not bind to the importin alpha homologues, suggesting that the N and P p

175 that the N protein binds to yeast and plant importin alpha homologues, whereas mutations in the carb

176 25-65 and includes the previously identified importin alpha IBB (alphaIBB) region of homology.

177 y requirement as compared with the classical importin alpha IBB.

178 eukaryotic nuclear import receptor subunit, Importin alpha (Imp alpha), and variant nuclear localiza

179 irect interactions of the Gag NC domain with importin-alpha (imp-alpha) and the MA domain with import

180 cargo directly or through an adaptor such as Importin-alpha (Impalpha).

181 The results indicate that freely diffusing importin alpha, importin beta, Ran and NTF2 are in dynam

182 We analyzed dynamic properties of importin alpha, importin beta, Ran and NTF2 in nucleus,

183 uclear import assay, both importin 7 and the importin alpha-importin beta heterodimer could import a

184 STAT6 seems to be mediated by the classical importin-alpha-importin-beta1 system.

185 of GST-NR1-1a abolishes its ability to bind importin alpha in brain lysates, and the interaction of

186 These results suggest a role for importin alpha in regulating lamin assembly and possibly

187 fied a role for the nuclear transport factor importin alpha in the regulation of lamin assembly.

188 binding assays, we show that it is bound by importin-alpha in almost the same manner and with simila

189 PMTV TGB1 interacted with importin-alpha in N. benthamiana, which was detected by

190 Our results define a new role importin-alpha in the stimulus-dependent control of sign

191 Both factors modulated lamin B3 import, with importin alpha increasing overall import rates and Ntf2

192 Exogenous importin alpha inhibited nuclear lamin assembly in Xenop

193 The C-terminal region (which binds importin-alpha) inhibits mitotic spindle pole formation.

194 tion of ChREBP.14-3-3 and inhibit the ChREBP/importin alpha interaction, resulting in cytosolic local

195 t and the amino-terminal domain required for importin-alpha interaction in plants, nucleolar targetin

196 te that the direct association of PABPC with importin alpha is antagonized by the presence of poly(A)

197 Binding of CREB2 to importin alpha is required for its transport from distal

198 Together, our results indicate that importin alpha is tethered at the postsynaptic density b

199 ignal (NLS) to the NLS receptor, karyopherin/importin alpha, is the most well studied nuclear transpo

200 siRNA knockdown of the human importin-alpha isoform KPNA2, corresponding to the murin

201 a isoform KPNA2, corresponding to the murine importin-alpha isoform previously shown to bind to DENV-

202 sensory neurons (SNs) and binds to specific importin alpha isoforms.

203 importin alpha1 and discuss the evolution of importin alpha isoforms.

204 on studies using DENV-2 and -4 NS5 and human importin-alpha isoforms failed to identify an interactio

205 t mice, to understand the role of individual importin-alpha isoforms in adaptation.

206 n (IFN) response by hijacking select nuclear importin-alpha isoforms.

207 os are preferentially imported by a distinct importin-alpha it remains unknown how this specificity i

208 try and repressor function, independently of importin alpha, its classical partner.

209 However, owing to the lack of importin-alpha knockout animal models in the past, their

210 iruses, in importin-alpha-silenced cells and importin-alpha-knockout mice, to understand the role of

211 and in higher eukaryotes there are multiple importin-alpha-like genes, but in fission yeast there ar

212 phages, NLS peptide specifically blocked the importin alpha-mediated nuclear import of NF-kappaB and

213 raction is needed for cell-to-cell movement, importin-alpha-mediated nucleolar targeting of TGB1 is a

214 eted to the nucleus by a Ran-independent and importin-alpha-mediated system.

215 The receptor importin-alpha mediates the nuclear import of functional

216 ays demonstrated that all tested Arabidopsis importin alpha members can interact with VirD2 and VirE2

217 Overexpression of six importin alpha members, including IMPa-4, rescued the ra

218 nteraction of Rb/p130 with importin beta and importin alpha, members of the nuclear transport machine

219 portin-alpha proteins but interact with rice importin-alpha more strongly.

220 Importin-alpha mutants that impair interactions during n

221 the characteristic nuclear import defect of importin-alpha mutants, whereas srp1-49 shows a defect i

222 not efficiently localized to the nucleus in importin-alpha mutants.

223 or importin-alpha, sequence variation at the importin-alpha NLS-binding sites and tissue-specific exp

224 CLIC4 associates with the Ran, NTF2, and Importin-alpha nuclear import complexes in immunoprecipi

225 d a specific interaction between Sgk and the importin-alpha nuclear import receptor.

226 ulting in disruption of its interaction with importin-alpha, nuclear exclusion, and subsequent degrad

227 However, Nup50 dynamics are independent of importin alpha, Nup153, and Nup98, even though the latte

228 The crystal structure of the importin-alpha:Nup50 complex shows that Nup50 binds at t

229 iated by the importin-beta binding domain of importin-alpha operates in plants, with NLS-mimicking se

230 he nuclear import factor Srp1 (also known as importin alpha or karyopherin alpha) is required for ubi

231 ural armadillo repeat proteins (nArmRP) like importin-alpha or beta-catenin bind their target peptide

232 ks Kap95p in a conformation that cannot bind importin-alpha or cargo.

233 etermine redundant and specific functions of importin-alpha paralogs from Arabidopsis thaliana.

234 Virus-induced gene silencing of two importin-alpha paralogs in Nicotiana benthamiana resulte

235 Plant genomes typically encode several importin-alpha paralogs that can have both specific and

236 mportin alpha, and activated in stage 8 when importin alpha partitions to a membrane pool.

237 Lamin assembly assays show that importin alpha prevents the self-association of lamins r

238 on between AtREM1.3 and four isoforms of the importin alpha protein family in a yeast two-hybrid syst

239 rained proteomimetics to target the HNF1beta-importin alpha protein-protein interaction were designed

240 s of mutants in the Saccharomyces cerevisiae importin alpha protein.

241 erned by the coordinated local expression of importin alpha proteins and ADAR protein variants.

242 calization signal sequences that may recruit importin alpha proteins of the plant for nuclear import.

243 decreases the interaction strength with the importin alpha proteins.

244 functional with plant, mammalian, and yeast importin-alpha proteins but interact with rice importin-

245 The gene structures of the importin alphas provide insight into how the animal impo

246 Importin beta then binds to the importin alpha-Rb2/p130 complex, leading to the transloc

247 It is known that importin alpha recognizes a NLS on a target protein and

248 , whereas Nup2p contributes to cargo release/importin alpha recycling through a distinct mechanism.

249 rminal importin beta binding (IBB) domain of importin alpha regulates NLS-cargo binding to the NLS bi

250 evidence that this auto-inhibitory-defective importin alpha remains bound to NLS-cargo within the nuc

251 esults are consistent with the view that the importin alpha's have both overlapping and distinct func

252 Importin alpha's mediate nuclear transport by linking nu

253 mal genomes encode three conserved groups of importin alpha's, alpha1's, alpha2's, and alpha3's, each

254 is stage for an activity shared by all three importin alpha's.

255 importin 8, bound both NL1 and NL2, whereas importin alpha selectively bound NL1.

256 Our results suggest that cargo affinity for importin-alpha, sequence variation at the importin-alpha

257 of their complexes with mouse (Mus musculus) importin-alpha show preferential binding to the major NL

258 stal structures of their complexes with rice importin-alpha show that they bind to the minor NLS bind

259 of avian and mammalian influenza viruses, in importin-alpha-silenced cells and importin-alpha-knockou

260 Similar to importin alpha, SNP1 uses an N-terminal importin beta bi

261 ortin-13) or through adaptor proteins (e.g., importin-alpha, snurportin-1, symportin-1), as well as u

262 Thus, differences in importin-alpha specificity are determinants of host rang

263 Here, we utilize two engineered mutants of importin-alpha/Srp1 with specific molecular defects to e

264 nuclear localization signal (cNLS) receptor, importin-alpha/Srp1, to the G(2)/M transition of the cel

265 S binding pocket of Saccharomyces cerevisiae importin alpha, Srp1p.

266 The importin alpha subunit recognizes classical NLS sequence

267 similar to those in the nuclear pore protein importin-alpha, suggesting that these E3s identify their

268 nt protein exhibited higher association with importin alpha than wild-type-p27.

269 The human genome encodes six isoforms of importin alpha that show greater than 60% sequence simil

270 ings indicate that CREB2 is a novel cargo of importin alpha that translocates from distal synaptic si

271 However, the part of the tryptophan array of importin-alpha that is essential for the recognition of

272 orresponding to NLS1 and NLS2 bind weakly to importin alpha, the two NLSs synergize in the context of

273 esized that TgGCN5 physically interacts with importin-alpha, the main transport receptor in the impor

274 the association of the NS1A RBD domain with importin-alpha, the protein that mediates nuclear import

275 interaction with the nuclear import receptor importin-alpha, thereby promoting cytoplasmic compartmen

276 n response to high glucose, ChREBP must bind importin-alpha; this heterodimer then forms a complex wi

277 Smad4 displays specific binding to importin alpha through its MH1 domain, which was abrogat

278 sequence (NLS) is prevented from binding to importin-alpha through intramolecular contacts between t

279 ylation inhibits the association of p27 with importin alpha thus preventing its re-entry into the nuc

280 eover, it has been shown that the binding of importin alpha to NLS significantly decreases with phosp

281 We show that the addition of importin alpha to purified lamin B3 prevents the assembl

282 al nuclear localization signal that recruits importin alpha to the Golgi membranes.

283 titively inhibited the ability of endogenous importin-alpha to import Sgk into nuclei in vitro.

284 Human cells encode several isoforms of importin-alpha; to determine whether any of these isofor

285 ironment that facilitates bringing Nup50 and importin alpha together, as well as other soluble factor

286 importin-alphas determine formation of cargo/importin-alpha transport complexes in plant cells.

287 that integrase coimmunoprecipitates with the importin-alpha transport receptor and directly binds to

288 zation signal-containing proteins depends on importin-alpha transport receptors.

289 rimarily binds the major-NLS binding site of importin alpha, unlike NLS1 that associates with the min

290 he Golgi matrix protein GM130 interacts with importin alpha via a classical nuclear localization sign

291 By inhibiting the Ran pathway with excess importin-alpha, we establish a role for chromatin-derive

292 ethylated H3 lysine 9 [H3K9me3]), and DIM-3 (importin alpha), which is involved in DIM-5 localization

293 uclear pore complex, and the adaptor protein importin alpha, which directly binds the classical NLS.

294 red for efficient association between NP and importin alpha, which is crucial for IAV RNP nuclear tra

295 107 amino acid carboxy-terminal fragment of importin-alpha, which contains the Sgk binding region, c

296 ltransferase is mediated by interaction with importin-alpha, which facilitates its transport and sele

297 terminal domain actively displaces NLSs from importin-alpha, which would be more consistent with Nup5

298 LS of Rb2/p130 leading to the interaction of importin alpha with Rb2/p130.

299 onstrated a strong and direct association of importin-alpha with endogenous Sgk and exogenously expre

300 alpha and is required for the interaction of importin-alpha with the NLS.