ライフサイエンスコーパス: initial velocity

1 ains both substrate and pH dependency of the initial velocity.

2 ranes (Michaelis constant, 20-40 nm; maximum initial velocity, 26-29 nmol g-1 fresh weight h-1), wher

3 The initial velocity (after 1-s microwave heating) of the bi

4 absence of the second Mg(2+) as evidenced by initial velocity analysis and substrate analogue and pro

5 m, random kinetic mechanism, as evidenced by initial velocity analysis indicating sequential addition

6 11 +/- 0.06 obtained by direct comparison of initial velocities and 1.2 obtained by single-wavelength

7 ere was a sigmoidal relationship between the initial velocity and bulk substrate concentration.

8 At low pH (5.7) the initial velocity and dead-end inhibition patterns are co

9 Initial velocity and dead-end inhibition studies support

10 Initial velocity and dead-end inhibitor studies with the

11 Initial velocity and inhibition kinetic studies support

12 trate binding to the enzyme, as evidenced by initial velocity and inhibition mechanism studies with n

13 Initial velocity and inhibition studies show that the st

14 Initial velocity and inhibition studies support a random

15 method for measurement of calcium influx at initial velocity and obviate concern for "unstirred laye

16 The results of initial velocity and product and dead-end inhibition exp

17 Initial velocity and product inhibition kinetic data are

18 Steady-state initial velocity and product inhibition kinetic studies

19 The steady-state initial velocity and product inhibition patterns are con

20 Initial velocity and product inhibition studies indicate

21 heterologously expressed and purified GmHSD, initial velocity and product inhibition studies support

22 Kinetic characterization, utilizing initial velocity and product inhibition studies, found t

23 licated profiles in the relationship between initial velocity and substrate concentration, making it

24 dispersion can be more important than large initial velocity and turbulent transport with dilute sus

25 NADPH is only 30% (initial velocities) as effective as NADH.

26 Under initial velocity assay condition of low product formatio

27 phorylation, were followed by monitoring the initial velocity at which the enzyme catalyzes the phosp

28 The IFE distortion of apparent initial velocities can be corrected without doing fluoro

29 Initial velocity data indicate the MgHIc complex is the

30 Global fitting analysis of initial velocity data indicates that a random terreactan

31 Comparison of the initial velocity data using AdoMet versus [2H3-methyl]Ad

32 Initial velocity data with DNA and S-adenosylmethionine

33 Initial velocity data with peptide and S-adenosyl-L-meth

34 H effects demonstrated in progress-curve and initial-velocity data sets from rat, human, Escherichia

35 Initial velocity determinations were conducted with huma

36 t of the desorbed ions, thereby reducing the initial velocity distribution and improving resolution.

37 cs we must include a sub-cycle change of the initial velocity distribution of the electron and its ex

38 Initial velocity experiments demonstrate that dimerizati

39 Equilibrium binding and initial velocity experiments revealed a less than 2-fold

40 From initial velocity experiments, catalytic constants for su

41 From initial velocity experiments, the Michaelis constant for

42 Kia value for this substrate determined from initial velocity experiments.

43 3D(pol) to obtain a reliable estimate of the initial velocity in as little as 4 min.

44 retained over a broad pH range, and apparent initial velocities indicate that maximal activities are

45 than those values determined from reciprocal initial velocity-initial substrate concentration plots f

46 inner filtering effect (IFE) alters apparent initial velocities, K(m), and k(cat).

47 Initial velocity kinetic studies indicated that Disperse

48 soybean root nodules has been determined by initial velocity kinetic studies monitoring oxygen uptak

49 for the synthetase from Escherichia coli by initial velocity kinetics and patterns of linear inhibit

50 ons per substrate molecule with very similar initial velocity kinetics and pH dependencies.

51 The initial velocity kinetics of these activities exhibited

52 On the basis of initial velocity kinetics, Tl+ enhances catalysis by 20%

53 spectroscopy, fluorescence spectroscopy, and initial velocity kinetics.

54 Initial velocity line patterns were also determined when

55 nstants for DXP synthase calculated from the initial velocities measured at varying concentrations of

56 Initial velocity measurements and inhibitor studies were

57 Initial velocity measurements have been used to measure

58 Initial velocity measurements indicated that the rate of

59 Yet, initial velocity measurements over the same temperature

60 ical approach using reaction timecourses and initial velocity measurements to determine the actual co

61 ERK2 was examined, with excess magnesium, by initial velocity measurements, both in the absence and p

62 ard and reverse reactions were determined by initial velocity measurements.

63 re substrates, as demonstrated by comparable initial velocity measurements.

64 o iminoribitol alone (28 pM vs 2.9 mM), from initial velocity measurements.

65 tic mechanism of AtGS was investigated using initial velocity methods and product inhibition studies.

66 Initial velocity methods were used to probe the kinetic

67 Initial velocities of [3H]mannose uptake were two- to th

68 Double reciprocal analysis of initial velocities of AcpS at various concentrations of

69 S) for the quantitative determination of the initial velocities of four heparin-modifying enzymes.

70 onventional steady-state kinetics as well as initial velocities of mixed substrate cleavages under th

71 Initial velocities of PC activation at different concent

72 of activation were assessed by measuring the initial velocities of the phosphorylation of the tyrosin

73 As predicted by the model, the initial velocity of 12-AS arrival at the acceptor membra

74 mbrane vesicles, translocase activity had an initial velocity of 3.3 nmol phosphatidylserine (PS) tra

75 The traditional method follows the initial velocity of [32P]PPi incorporation into ATP by c

76 that propagated along the arteriole with an initial velocity of approximately 116 microm/s (n=28) an

77 of unbound analyte to be determined from the initial velocity of binding.

78 distinct functional consequences: increased initial velocity of fibrin clot formation, altered fibri

79 reactions of selenoxide 8 with H(2)O(2).The initial velocity of oxidation of PhSH by H(2)O(2) with s

80 ral correlate of these neural responses: the initial velocity of pursuit eye movements deviates in a

81 EhUba1 exhibits a greater maximal initial velocity of pyrophosphate:ATP exchange than its

82 We show that while the initial velocity of the assembly depends on Tim9 concent

83 g phosphatidylcholine/cholesterol, 1:1), the initial velocity of the mutant is reduced 3000-fold.

84 product scatters forward with respect to the initial velocity of the O atom, and the H atom scatters

85 d under the same conditions by following the initial velocity of the phosphorylation of peptides cont

86 xamination of transporter kinetics utilizing initial velocity of uptake revealed that METH treatment

87 The initial velocity of VPA uptake varied in proportion with

88 DYNAFIT was developed for fitting either the initial velocities or the time course of enzyme reaction

89 The initial velocity pattern in which the ratio between the

90 A parallel initial velocity pattern that displays competitive subst

91 Intersecting initial velocity patterns for both glucose and ATP indic

92 Data from initial velocity patterns in the presence and absence of

93 mechanism for JNK3alpha1 was determined via initial velocity patterns in the presence and absence of

94 and fixed concentrations of oxamate revealed initial velocity patterns inconsistent with a simple pin

95 Initial velocity patterns indicate that both domains fol

96 Initial velocity patterns indicate that both domains fol

97 Analysis of the initial velocity patterns indicated a sequential mechani

98 Parallel initial velocity patterns indicated that both substrates

99 The initial velocity patterns indicated that the kinetic mec

100 Bi-substrate kinetic analysis gave initial velocity patterns indicating a sequential mechan

101 Analysis of initial velocity patterns suggests a random sequential k

102 Initial velocity patterns were consistent with a sequent

103 ocal plots with UTP produced parallel lines, initial velocity plots with other phosphate donors and p

104 nd PPi is the last product released based on initial velocity, product and dead-end inhibition studie

105 substrate were used to perform steady-state initial velocity, product inhibition, and dead end inhib

106 Initial velocity, product inhibition, and dead-end analo

107 Initial velocity, product inhibition, and dead-end inhib

108 Using a combination of initial velocity, product inhibition, and dead-end inhib

109 lucokinase reactions were investigated using initial velocity, product inhibition, and dead-end inhib

110 Initial velocity, product inhibition, and dead-end inhib

111 Initial velocity, product inhibition, and deuterium kine

112 Initial velocity, product inhibition, dead-end inhibitio

113 Thus, initial velocity, product, and dead-end inhibition data

114 Finally, the results of initial velocity, product, and dead-end inhibition studi

115 The results of initial velocity, product, and dead-end inhibition studi

116 Initial velocity, product, and dead-end inhibition studi

117 Initial velocity profiles were consistent with an ordere

118 The initial velocity results were equally consistent with in

119 o the starting thiol ester was detected with initial velocity solvent isotope exchange experiments.

120 ed friction has complex evolution, featuring initial velocity strengthening followed by substantial v

121 Initial velocity studies confirm the change in mechanism

122 Initial velocity studies demonstrate that APH(2'')-Ib op

123 bserved in both isotope exchange studies and initial velocity studies has not yet been identified.

124 Isotope partitioning and initial velocity studies have been used to study the kin

125 carboxylation of isocitrate, on the basis of initial velocity studies in the absence and presence of

126 accharomyces cerevisiae was determined using initial velocity studies in the absence and presence of

127 analysis of PAP was conducted which included initial velocity studies of the forward and reverse reac

128 Initial velocity studies reveal that Rv2747 proceeds thr

129 Initial velocity studies revealed that all AKT isozymes

130 Initial velocity studies show that EntE proceeds via a b

131 Initial velocity studies show that IIAGlc is an uncompet

132 Initial velocity studies show the labeling does not alte

133 dD33 prefers long chain saturated lipids and initial velocity studies showed that FadD33 proceeds via

134 Initial velocity studies support an ordered kinetic mech

135 Initial velocity studies were consistent with a sequenti

136 ctivator, was present at a saturating level, initial velocity studies were consistent with a Theorell

137 Initial velocity studies with L-glutamate showed that ev

138 Site-directed mutagenesis, initial velocity studies, pH-rate studies, and substrate

139 Initial velocity studies, product inhibition, and dead-e

140 On the basis of initial velocity studies, the selectivity of the activat

141 An initial velocity study indicated an ordered bi-bi cataly

142 Initial velocity techniques were used to define the stea

143 40 microM are biphasic and characterized by initial velocities that decay by a first-order process t

144 isulfide formation in a redox buffer with an initial velocity that is 30-fold faster than PDI.

145 Comparing the simulated activity (ratio of initial velocity to the enzyme concentration) under phys

146 iaryltellurides 3 and 4 as determined by the initial velocities, v(0), for the rate of appearance of

147 Double-reciprocal plots of initial velocities versus the varying concentrations of

148 Plots of initial velocity versus free magnesium concentration are

149 their values determined from the reciprocal initial velocity versus initial substrate concentration

150 Plots of initial velocity versus the concentration of the varied

151 A set of initial velocities was analyzed to see if a tight-bindin

152 The temperature dependence of initial velocities was used to estimate activation param

153 Although the measured initial velocity was higher when either fragment 2 or fr

154 the kinase domain of v-fps (GST-kin) and the initial velocities were determined by a coupled enzyme a

155 ved that the extent of hybridization and the initial velocities were directly dependent on the length

156 However, the observed initial velocities were too low to accurately determine