ライフサイエンスコーパス: insertion

1 sible phase transition upon K extraction and insertion.

2 fin cyclopropanation and heteroatom-hydrogen insertion.

3 , we have uncoupled satellite growth from NE insertion.

4 sues by selective distal swelling after skin insertion.

5 of CIA2A, CIA2B, and MMS19 for Fe/S cluster insertion.

6 ells that serve as a blueprint of TA protein insertion.

7 ly the inefficiency of targeted DNA cassette insertion.

8 were studied prior to and 1-hour after TIPSS insertion.

9 All complexes are well poised for dCTP insertion.

10 to -1.56 diopters) was also noted after ICRS insertion.

11 ates an energy barrier that impedes membrane insertion.

12 ansthoracic echocardiography during catheter insertion.

13 pies prevents reliable quantification of new insertions.

14 ersity of chromosomal sites harboring new RT insertions.

15 shares features with reported oncogenic DNA insertions.

16 fission regardless of the hydrophobicity of insertions.

17 arge proportion of which are retrotransposon insertions.

18 matches, abasic sites, and single nucleotide insertions.

19 shape, structure, stability, slit diaphragm insertion, adhesion, plasticity, and dynamic response to

20 generates correctly targeted conditional and insertion alleles in 8.5-100% of the resulting live offs

21 le, generating both conditional and targeted insertion alleles.

22 structural features that facilitate membrane insertion and channel formation, now aligning ion channe

23 theta pathway, Poltheta carries out both the insertion and extension steps of TLS opposite 3-dMeA, an

24 We show that controlling the insertion and extraction of oxygen and hydrogen ions ind

25 xcellent electrochemical performance for the insertion and extraction of potassium ions.

26 machineries at the time of initial membrane insertion and folding.

27 As) serve as very efficient donors, both for insertion and for gene replacement.

28 Slippage directionality, base insertion and omission, is sensitive to the relative con

29 teins facilitating oligomerization, membrane insertion and pore formation.

30 e unstrained C-C bond, followed by migratory insertion and reductive elimination.

31 The resulting rapid membrane insertion and sensitizing ability provide PTTP with a hi

32 e alleles of AvrSr50 have arisen through DNA insertion and sequence divergence, and our data provide

33 study provides the basis for TIRAP membrane insertion and the mechanism by which it is removed from

34 the aryl ring control the rate of the alkene insertion and the regioselectivity of the catalytic reac

35 at are recognized and decoded by the protein insertion and translocation machineries at the time of i

36 id increase with age in the number of random insertions and a dramatic increase in diversity.

37 ncreases the frequency of both smaller-scale insertions and deletions (indels) and of single-nucleoti

38 Small insertions and deletions (indels) have a significant inf

39 iants (SNVs), with the contribution of small insertions and deletions (indels) less well characterise

40 erns of modified bases, including miscoding, insertions and deletions (indels), and truncations, to u

41 ds to accumulation of large numbers of small insertions and deletions (indels).

42 frame, such as stop-lost variants and small insertions and deletions (InDels).

43 AP in that it was more likely to codon align insertions and deletions and to facilitate the placement

44 n be modified to facilitate the placement of insertions and deletions at specific positions.

45 (IDAA) determines the size and frequency of insertions and deletions elicited by nucleases in cells,

46 single nucleotide variants and 12.6 de novo insertions and deletions or copy number variations per A

47 Surprisingly, indel (insertions and deletions) percentages averaged nearly 70

48 nst the wild-type sequence revealed multiple insertions and deletions.

49 nucleotide polymorphisms, and short in-frame insertions and deletions.

50 rated great sensitivity in identifying short insertions and deletions.

51 d to disrupt gene function by inducing small insertions and deletions.

52 INOM also detected an association between L1 insertions and gene bodies marked by a facultative heter

53 significant pairwise association between L1 insertions and heterochromatic marks.

54 sposon mutant library of >430,000 unique Tn5 insertions and measured mutant depletion upon exposure t

55 oceeding via fluoride abstraction, migratory insertion, and C-F reductive elimination to achieve a ne

56 totally, including 20,328 substitutions, 88 insertions, and 154 deletions, compared to the EBV refer

57 out-of-plane distances of 1 to 15 mm, and at insertion angles of 33 to 42 degrees relative to the pro

58 Transgenic mice generated by random genomic insertion approaches pose problems of unreliable express

59 Among these non-coding variants, somatic insertions are among the least well characterized due to

60 New LINE-1 insertions are thought to accumulate mostly during human

61 helical scaffolds, and hydrophobic membrane insertions are thought to be the primary drivers of fiss

62 nful experiences (e.g., skin punctures, tube insertions) are associated with reduced brain responses

63 ment with the damage avoidance hypothesis of insertion, as they presumably limit the overall net push

64 including: (1) efficient targeted transgene insertion at safe harbour loci (Rosa26 and AAVS1); (2) b

65 t of an important drug-resistance associated insertion at the position at which most laboratories exp

66 stematic quantitative analysis of glial cell insertions at central nodes of Ranvier.

67 ctural design principle for this divergence: Insertions at key positions in the minimal scaffold that

68 The (H2O)3Ne + H2O ring insertion barrier is sufficiently large, such that evapo

69 INOM to explore L1 retrotransposable element insertion bias in lung cancer and found a significant pa

70 New L1 insertions bore structural hallmarks of target-site prim

71 of pathway components to facilitate membrane insertion, but the physiological significance in multice

72 hway, Polzeta extends synthesis following nt insertion by an as yet unidentified Pol.

73 AV) donor vectors enables site-specific gene insertion by homology-directed genome editing.

74 Beyond membrane insertion by the C2 domains, other requirements for Syna

75 s and discovered that RAG1/2 causes aberrant insertions by releasing cleaved antibody gene fragments

76 t prevalent RP-causing mutations are the Alu insertion (c.1297_8ins353, p.K433Rins31*) in the male ge

77 in, it was found that the pH switch sequence insertion caused minimal change at pH 8 but a ca. 50 deg

78 several unintegrated OmpLA mutants remained insertion-competent, they were slowly degraded by the pe

79 all defect at the base of the umbilical cord insertion containing the herniated abdominal organs incl

80 This paper discusses progress in the area of insertion-deletion models, in view of recent work by Eza

81 esults were obtained for 10,641,224 SNPs and insertion-deletion variants with minor allele frequencie

82 landscape of quiescence is characterized by insertion/deletion (indels) accumulating as fast as sing

83 Each gene has a coding sequence insertion/deletion mutation within the SC species that p

84 rising multiple nearby base substitutions or insertion-deletions.

85 , single-nucleotide polymorphisms (SNPs) and insertions/deletions (indels) can result in altered on-t

86 ased with increasing irrigant flow rates and insertion depths of a 30-gauge side-venting needle.

87 cribe a novel method based on intronic MiMIC insertions described in Nagarkar-Jaiswal et al. (2015) t

88 We suggest that this insertion determines the topology of the K2 FERM domain,

89 GeV electron beam through a two-stage plasma insertion device.

90 These insertions did not negatively impact the sequence stabil

91 s, introduced by increased deletions/reduced insertions during VDJ rearrangement.

92 This insertion encodes a predicted protease with 54 to 68% am

93 smaller promoter-enhancer loops, whereas PB insertion encompasses larger chromatin loops termed topo

94 pair have left a mutational footprint on the insertion environment of mobile introns or nonrandom pat

95 rimer terminus (3-end) during the succeeding insertion event.

96 ow that the material is associated with both insertion/extraction and conversion reaction mechanisms

97 change optical properties by electric charge insertion/extraction.

98 The frequency of targeted insertion for these Cpf1 nucleases, up to 8%, is higher

99 nd repeatable way with quantification of the insertion force and velocity.

100 However, AnkG did alter ENaC insertion from constitutive recycling pathways.

101 y; recombination events that delete the URA3 insertion from the CUP1 array occur at a rate of >10(-3)

102 This insertion generated, through somatic mutations, broadly

103 n study, we identified a naturally occurring insertion in a regulatory DNA element in ss that lowers

104 ting signal sequence and transmembrane helix insertion in a substrate-dependent manner.

105 ied a complex homozygous 4-kb deletion/20-bp insertion in DSTYK (dual serine-threonine and tyrosine p

106 The HAs of H15 viruses contain an insertion in the 150-loop (loop beginning at position 15

107 ng site common to this subgroup and a unique insertion in the 260-loop compared to any other subtype.

108 e variation from 10 to 110 cm(3)/g by cobalt insertion in the MoS2 crystallographic lattice, which in

109 III predominance, and lack of collagen fiber insertion in the necrotic bone were associated with impa

110 To determine if IVC filter insertion in trauma patients affects overall mortality.

111 prospective multicentre cohort study of EVD insertions in 21 neurosurgical units was performed over

112 rint that can create in-frame and frameshift insertions in coding sequences.

113 An ordered Tn library in strain AB5075 with insertions in every nonessential gene was subjected to s

114 the possible biological impact of somatic L1 insertions in neurons, the existence of donor L1s that a

115 mammary tumors, most of which had transposon insertions in one of two RASGAP genes, neurofibromin1 (N

116 AS-OCT is valuable in identifying EOM insertions in primary strabismus surgeries, but the accu

117 ten cause exon skipping, short deletions, or insertions in the mature mRNA, underlying one common mol

118 Cellular allele frequencies of examined L1 insertions indicated heterozygosity in genomes of DLPFC

119 hat changes in base composition and backbone insertions influence the translocation rates, with incre

120 1, which readily performs an intramolecular insertion into a C horizontal lineC bond of its aromatic

121 to a Ni(0) catalyst and proceeds via alkene insertion into a Ni(II)-acyl bond.

122 distinguished from soluble proteins by their insertion into biological membranes.

123 spacer-sized fragments and channels them for insertion into CRISPR array.

124 wild type opsin which dimerizes en route to insertion into phospholipid vesicles, QUAD opsin reconst

125 ckland metabolism and found that a TargeTron insertion into selD, encoding the selenophosphate synthe

126 Similarly, reactive center loop insertion into sheet A decreased heparin binding because

127 2, the alpha-C-C coupling of 1a, and the CO2 insertion into the alpha-C-H sigma-bond of amine 1a.

128 boronic esters with formal 1,1'-benzylidene insertion into the C-B bond.

129 including conjugation to the apolipoprotein, insertion into the phospholipid layer, and loading into

130 and caveolae-associated vesicles prior to re-insertion into the plasma membrane.

131 -Src-specific microexon leads to a 5 or 6 aa insertion into the SH3 domain of Src.

132 Seeksv can detect DEL, insertion, inversion and inter-chromosome transfer at si

133 External ventricular drain (EVD) insertion is a common neurosurgical procedure.

134 Second, it combines insertion-level evidence for the corresponding gene.

135 gh construction of complex random transposon insertion libraries and quantification of each mutant's

136 Using a highly saturated transposon insertion library combined with next-generation sequenci

137 s hypothesis, we used a bar-coded transposon insertion library in tandem with cell sorting to assess

138 The single transfer DNA insertion lines CRK28 and CRK29, two CRKs induced in res

139 re than 3700 confirmed homozygous SALK T-DNA insertion lines for visible defects under prolonged grow

140 arried out in independent L. japonicus LORE1 insertion lines indicates a positive role of LjNPF8.6 on

141 Thermotolerance assays of PME gene T-DNA insertion lines revealed two null mutant alleles of PME3

142 f 96% at 0.25 THz carrier frequency with low insertion loss and device length as small as 100 microns

143 xtinction ratios as high as 42 dB along with insertion losses as low as 0.18 dB.

144 eloped inexpensive methods for INTACT, T-DNA insertion mapping, and profiling of the complete nuclear

145 icroscopy to directly visualize the membrane-insertion mechanism.

146 However, the activation and membrane-insertion mechanisms have remained elusive.

147 The MEF8 T-DNA insertion (mef8) line exhibited reduced editing at 38 mi

148 Mobile element insertions (MEIs) represent approximately 25% of all str

149 After SPB insertion, membrane-associated proteins including the co

150 Our results suggest that ancestral TE insertions might have brought in cis-regulatory modules

151 ssion, and thus there is a need for targeted-insertion models.

152 te the extremely large progenitor transposon insertion mutant collections needed to achieve saturatin

153 A chlamydial transposon insertion mutant in the Cdu1-encoding gene exhibited inc

154 sis (Arabidopsis thaliana) CI mutants: a new insertion mutant interrupted in both ndufs8.1 and ndufs8

155 Exposing a saturating transposon insertion mutant library of S Typhimurium to immune seru

156 Analyses of T-DNA insertion mutants for each of these candidate PSS1 genes

157 functionally complementing Arabidopsis T-DNA insertion mutants in this gene.

158 Functional studies using T-DNA insertion mutants reveal that they can act as antisense

159 Insertion mutations in EGFR and HER2 both occur at analo

160 also shows an example of how a TRIM element insertion near a gene can contribute to genetic novelty

161 MbR contains a unique 29-amino acid insertion near the C terminus, which folds as a separate

162 Insertion of a 9-residue hemagglutinin epitope in IS1S2,

163 IRP1 RNA binding by iron primarily involves insertion of a [4Fe-4S] cluster by the cytosolic iron-su

164 would limit efficient chain elongation after insertion of a fluorinated monomer.

165 These results demonstrate that insertion of a full-length protein into non-CDR loops of

166 Moreover, insertion of a GL promoter downstream of the CTCF insula

167 functionalization of the AvrSr35 gene by the insertion of a mobile element.

168 BIM)(CNAr(Dipp2) )] can effect the oxidative insertion of a range of unsaturated organic substrates,

169 was investigated in cynomolgus monkeys after insertion of a roughened catheter into either the vena c

170 1 and four-membered cyclic silylene (4) via insertion of a silicon(I) atom of 2 into the Cphenyl-N b

171 was investigated by monitoring the membrane insertion of a tail-anchored protein, SYP72, a syntaxin.

172 en modified by DCCD interferes with the deep insertion of a Tat signal peptide into the TatBC recepto

173 ated whether a mutation in the CX3C motif by insertion of an alanine, A(186), within the CX3C motif,

174 ents support a mechanism involving selective insertion of an alkynylboronate to a Co-B bond of a coba

175 d a disrupted DNA repair mutY gene caused by insertion of an integrative and conjugative element.

176 We go on to show that insertion of an ultrathin CTE buffer layer mitigates thi

177 ion of trajectories for the co-translational insertion of arbitrary polypeptide sequences into the Se

178 omplexes that participate in rapid migratory insertion of carbenes derived from silyl- or carbonyl-st

179 er new synthetic opportunities for migratory insertion of carbon-based species into gold-carbon bonds

180 Migratory insertion of carbon-based species into transition-metal-

181 Indeed, insertion of CD22 ligands into the RBC cell surface stro

182 SiMe3)(L3) (L = MeC(N(i)Pr)2) (2), undergoes insertion of chalcogen atoms resulting in a series of th

183 y in mammalian cells frequently requires the insertion of disulfide bonds.

184 functionally interchangeable with Alx1, but insertion of Domain 2 conferred robust skeletogenic func

185 ency for creating models that require either insertion of exogenous DNA (knock-in) or replacement of

186 Here, the pathways diverge with the insertion of Fe(2+) or Mg(2+) into protoporphyrin by fer

187 The treatments involve the insertion of fine needles at acupoints along specific me

188 d intermediate with fluoromalonyl-CoA allows insertion of fluorinated extender units at 43% of the yi

189 Insertion of foreign molecules such as functionalized fl

190 evoked currents and impaired NMDAR-dependent insertion of GluA1 at stimulated synapses.

191 Activity at synapses triggers insertion of GLUT4 into the axonal plasma membrane drive

192 Intercalation, the insertion of guest atoms, ions or molecules between the

193 The insertion of helium gas into CaZrF6 both elastically sti

194 rone HemW from bacteria, is required for the insertion of heme b into respiratory chain enzymes.

195 emW/RSAD1 is a heme chaperone catalyzing the insertion of heme into hemoproteins.

196 We provide evidence of cooperative insertion of homo-oligomeric complexes and demonstrate t

197 secretion across the cytoplasmic membrane or insertion of integral membrane proteins into the phospho

198 emonstrate that COX20 stabilizes COX2 during insertion of its N-proximal transmembrane domain, and su

199 ied EMC in synthetic liposomes catalyzed the insertion of its substrates in a reconstituted system.

200 Cotranslational insertion of membrane proteins into defined nanoparticle

201 oteins, whereas the Oxa1 is required for the insertion of mitochondria-encoded membrane proteins.

202 alent lithium or sodium ions, the reversible insertion of multivalent ions such as Mg(2+) and Al(3+)

203 ll molecules across the nucleic envelope via insertion of nanopores into the bilayers.

204 res hydrolases to cleave the cross-links for insertion of nascent PG material.

205 us, FtsZ treadmilling guides the progressive insertion of new cell wall by building increasingly smal

206 Amber codon suppression for the insertion of non-natural amino acids (nnAAs) is limited

207 al heteroligomer that catalyzes the membrane insertion of OMPs.

208 Our approach may be adapted to direct the insertion of other membrane proteins into vesicles.

209 We have revealed the first aspects of the insertion of proteins into nanodiscs by combining cell-f

210 Their chemical structure allows the insertion of reporter molecules and surface-binding agen

211 When intelligibility was restored with the insertion of silence gaps, LRTC in the delta, theta, and

212 suggest that channel activation may involve insertion of STIM1 into the PM.

213 ainst infected erythrocytes and suggest that insertion of templated DNA represents an additional mech

214 ntaining, zwitterionic silylene ring (3) via insertion of the "Si(I)2" unit of 2 into the olefinic C-

215 for hydroboration of the internal alkene is insertion of the alkene into a copper(I) hydride formed

216 reactive dichlorocarbene, due to competitive insertion of the carbene into the N-N bond.

217 onserved amino acids, but can be restored by insertion of the corresponding motif of Arabidopsis CENP

218 actors and represents a new paradigm for the insertion of the Fe/S cofactor into a radical SAM protei

219 alculating the charge transfer upon membrane insertion of the HIV gp41 fusion peptide, and validate t

220 we introduce a simple approach to orient the insertion of the light-driven proton pump proteorhodopsi

221 loaded activation mechanism through rational insertion of the light-sensitive LOV2 domain that expand

222 Following insertion of the MNA into the skin, individual microneed

223 brane proteins play an essential role in the insertion of the new SPB into the NE.

224 from within the nucleus is important for NE insertion of the new SPB.

225 se reactions occur with chemoselectivity for insertion of the nitrene units into C-H bonds over reduc

226 somal biogenesis factor 5 (PEX5) followed by insertion of the PEX5-cargo complex into the peroxisomal

227 HC reduces insertion of the stress-regulated exon (STREX) in the KC

228 The insertion of thiol-oligonucleotides into p-ATP monolayer

229 Genetic insertion of this tag resulted in viable replicons and s

230 Insertion of two glycines into the Rieske domain and sub

231 e intasome nucleoprotein complex to catalyze insertion of viral DNA into cellular chromatin.

232 genome editing has enabled the mutation and insertion of virtually any DNA sequence, particularly in

233 V vaccine in vitro and in vivo We found that insertions of foreign genes, such as the RSV F and G gen

234 to the Ae. tauschii Gli-2 region, including insertions of high numbers of non-syntenic genes and a h

235 The origins and insertions of the OPL were relatively similar, but its o

236 itial insight into the biological effects of insertions on candidate cancer genes.

237 PDGFRA mutations and KIT exon 11 insertion or duplication mutations were associated with

238 xhibit high rates of mutation in the form of insertions or deletions (indels) of the repeated motif.

239 h temocillin MIC was </=512 mg/L (nucleotide insertions or deletions, premature termination, tandem r

240 e edited sequences contain various imprecise insertions or deletions.

241 10% nucleotide somatic hypermutation and no insertions or deletions.

242 ure (blunt-ended junctions or junctions with insertions or microhomology).

243 lly, our data suggest that many heritable L1 insertions originate from individuals with partially com

244 usly thought to drive fission by hydrophobic insertion, our results show that membrane coverage corre

245 Our findings reveal a novel RAG1/2-mediated insertion pathway distinct from DNA transposition and tr

246 y mechanistic study favors an Ir-C migratory insertion pathway.

247 Here we found that known membrane insertion pathways fail to effectively engage tail-ancho

248 y of membrane proteins necessitates multiple insertion pathways that remain incompletely defined.

249 defects, suggesting presence of alternative insertion pathways while highlighting an intricate invol

250 The pH-low insertion peptide (pHLIP) is a leading peptide technolog

251 oenvironment, PNAs were conjugated to pH-low insertion peptide (pHLIP).

252 may have increased numbers of deleterious L1 insertions, perhaps occurring de novo, in brain-expresse

253 include recognition that the FGF4 retrogene insertion, previously associated with appendicular chond

254 ion by undergoing a coupled folding/membrane insertion process.

255 The conventional O-H insertion product is essentially suppressed by judicious

256 ne K2Pentacene and K2Picene by a solid-solid insertion protocol that uses potassium hydride as a redo

257 ees of C57BL/6J animals, and uncovered an L1 insertion rate of >/=1 event per eight births.

258 e, alpha-MnO2 can be applied to a cathode by insertion reaction and to an anode by conversion reactio

259 An efficient copper-catalyzed carbenoid insertion reaction of alpha-diazo carbonyl compounds int

260 fashion through photochemically induced C,H insertion reactions and consists of a protective layer,

261 enhance selectivity and capacity via sodium insertion reactions, carbon aerogel electrodes were modi

262 ormed and undergoes selective CH, NH, and OH insertion reactions, cyclopropanation, cyclopropenation,

263 TIPSS insertion renders this neutrophil functional defect syst

264 The insertion sequence (IS) elements are the smallest but mo

265 a 3 untranslated region (UTR) selenocysteine insertion sequence (SECIS) and the SECIS-binding protein

266 Nearly 40% of the prophages harbored insertion sequences (IS) previously described in H. pylo

267 g genomic deletions of 3' UTR selenocysteine-insertion-sequences (SECIS1 and SECIS2).

268 also report that the TL and the TL sequence insertion (SI3) increase overall termination efficiency

269 Selection of central venous catheter insertion site in ICU patients could help reduce cathete

270 cing allows affordable ultra-deep transposon insertion site recovery in high-throughput formats withi

271 ar magnetic resonance at the right ventricle insertion site.

272 nem or ciprofloxacin) by Transposon Directed Insertion-site Sequencing (TraDIS).

273 m that detects both new transposable element insertion sites and their methylation states from a sing

274 In the analysis of these screens, transposon insertion sites are typically identified by targeted DNA

275 Thirty-seven per cent (9754) of these insertion sites are within genes (including untranslated

276 This increased the number of unique insertion sites in the T-DNA collection by 21 078, bring

277 6 synonymous nucleotide changes, and a 12-bp insertion strongly associated with the E7 T20I/G63S vari

278 results reveal different modalities of LAIR1 insertion that lead to public and dominant antibodies ag

279 tereochemistry followed by reversible olefin insertion to form a (cyclopropylcarbinyl)nickel complex,

280 4 (Ar=2,6-(i) Pr2 C6 H3 ) or undergo nitrile insertion to give diazametallocyclobutene 8 (Ar=4-Ph-2,6

281 ing the distance of extraocular muscle (EOM) insertion to the limbus to improve preoperative assessme

282 ltrasound can reduce central venous catheter insertion to use time, exposure to radiation, and improv

283 We traced heritable L1 insertions to pluripotent embryonic cells and, strikingl

284 lost their ability to replicate, those whose insertion took place more recently, such as the HML-2 gr

285 nd restricts the regulatory influence of new insertions toward neighboring chromosomal DNA.

286 which could explain the observed bias for L1 insertions towards cancer-associated genes.

287 such as chromosomal rearrangements, deletion/insertion, transposon-mediated dispersion, or possibly b

288 repair (MMR), suppresses telomeric sequence insertion (TSI) at intra-chromosomal regions.

289 Prolonged dwell time and catheter insertion under emergent conditions increased risk of PV

290 d here to identify enhancer-associated small insertion variants provides a foundation for further stu

291 ne if there are instances of polymorphic Alu insertion variants that function in a common variant, co

292 guided, right-sided, central venous catheter insertion verifies proper placement and shortens time to

293 ikely accommodate the more numerous sites of insertion versus deletion editing as editosomes collabor

294 alleles in zebrafish by generating intronic insertions via in vivo homologous recombination.

295 Insertion was achieved by balancing the thermodynamic dr

296 of potential polar effects of the TargeTron insertion, we developed a CRISPR-Cas9 mutagenesis system

297 CML38-knockout mutants generated via T-DNA insertion were insensitive to AtRALF1, and simultaneous

298 Consistently, Tol2 insertions were associated with bivalent histone modific

299 atenated sulfur atoms with or without oxygen insertion, which accumulate in the absence of the His(64

300 We found over-representation of L1 insertions within the gene ontologies 'cell projection'