ライフサイエンスコーパス: insertion site

1 ar magnetic resonance at the right ventricle insertion site.

2 disruption was observed at the iStent inject insertion site.

3 ce, 23-25 nucleotides upstream of the intron insertion site.

4 position immediately adjacent to the intron insertion site.

5 ) and adjacent chromosomal DNA at the SCCmec insertion site.

6 FP reporter was inserted 5 kb from the bw(D) insertion site.

7 on of the hydrophobic core of Bn near the Ub insertion site.

8 pears to be a base preference (TTCT) for the insertion site.

9 ns for GC content and nucleotide bias at the insertion site.

10 of a sponge species, which also has the same insertion site.

11 of two conserved tyrosine residues into the insertion site.

12 ons should be targeted to the chosen genomic insertion site.

13 rotein binds DNA sequences downstream of the insertion site.

14 DNA sequences upstream and downstream of the insertion site.

15 DNA polymerase genes surrounding the intron insertion site.

16 lecule with 50 bp of homology specifying its insertion site.

17 keep track of sequences, mappings and common insertion sites.

18 irs and higher orders of co-occurring common insertion sites.

19 ted host carries between 500 and 5000 unique insertion sites.

20 monin mechanically reinforces tip link upper insertion sites.

21 c regions that coincided with preferential P-insertion sites.

22 er the chromodomain contributed to selecting insertion sites.

23 and robust system for identifying transposon insertion sites.

24 tion of cis-acting elements at these several insertion sites.

25 activation of genes flanking the retroviral insertion sites.

26 irrespective of the chromosomal loci of the insertion sites.

27 of the hyper-variable surface next to splice insertion sites.

28 s, and multiple strategies exist for mapping insertion sites.

29 asked region near the microdialysis catheter insertion sites.

30 characterized an unprecedented number of hsr insertion sites.

31 q by in-depth characterization of individual insertion sites.

32 exogenous sequences that sometimes surround insertion sites.

33 cause it contributes to the selection of new insertion sites.

34 could be distinguished due to differences in insertion sites.

35 occlusion onto the skin surrounding catheter insertion sites.

36 The friend leukemia insertion site 1 (Fli-1) transcription factor, an Ets fa

37 or vascular complications at the 18-F sheath insertion site (2.1% vs. 11.3%; p = 0.003), and of all t

38 retroviral insertion at the ecotropic viral insertion site 32 locus leads to increased expression of

39 selected mutants and found that 33 out of 44 insertion sites (75%) could be confirmed by PCR, and 17

40 a uniform distribution of new peptidoglycan insertion sites, a necessary condition to maintain rod s

41 be used to determine gene involvement at the insertion site after FISH has identified the presence of

42 provides a graphical overview of the mapped insertion sites against a karyotype.

43 f echocardiographic scar at papillary muscle insertion sites (all P<0.05) and, when combined, were ad

44 e the diversity of genomic islands and their insertion sites among Gram-negative bacteria and discuss

45 differences in the methylation status of the insertion sites among MITE families.

46 stion-based approaches for ligation-mediated insertion site amplification.

47 Insertion site analyses of recently reported trials on a

48 Insertion site analysis demonstrated that more than 80%

49 Vector insertion site analysis of granulocytes demonstrated sus

50 Insertion site analysis of the library repeatedly identi

51 e timing of methylation is influenced by the insertion site and by additional genetic information.

52 he sole variables were transgene chromosomal insertion site and copy number.

53 lineage demonstrated a preferred chromosomal insertion site and different complements of non-LEE enco

54 pport the preferential use of the subclavian insertion site and enhanced efforts to reduce dressing d

55 is approximately 4.7 kb upstream of the Mu1 insertion site and may be proximal to an adjacent repeat

56 Identification of the activation tag insertion site and microarray analysis revealed that ATH

57 n evolutionarily conserved fragment near the insertion site and observed enhancer activity of this el

58 QRS expression dependent on the ventricular insertion site and often coexisted with other tachycardi

59 Linkage between the insertion site and phenotype was confirmed, and we show

60 cing (INSeq) is a method for determining the insertion site and relative abundance of large numbers o

61 fered in several aspects, such as the intron insertion site and sequence length.

62 he tla2 phenotype, was cloned by mapping the insertion site and upon complementation with each of the

63 Varying the HPIV3 antigen insertion site and vector dose allowed fine-tuning of th

64 s mapped to millions of potential transposon insertion sites and a large portion of insertion sites h

65 Determination of insertion sites and adjacent viral sequences identify th

66 e, we describe a method to recover transgene insertion sites and assess structural rearrangements of

67 lengths observed in each of a collection of insertion sites and is maximized with a hybrid expectati

68 This allowed us to identify the insertion sites and phenotypes of negatively selected mu

69 We present the full genomic sequences, insertion sites and phylogenetic analysis of 28 prophage

70 by cryogenic storage, (2) identify mutagenic insertion sites and physical coordinates in these collec

71 tant mice also had improper ureteral bladder insertion sites and shortened intravesicular tunnel leng

72 Distances between the rotator cuff insertion sites and the glenoid decreased in the loaded

73 m distances were computed between the tendon insertion sites and the glenoid, acromion, and coracoid

74 m that detects both new transposable element insertion sites and their methylation states from a sing

75 asis genes were identified by sequencing the insertion sites and then mapping these sequences back to

76 ning, collection method, needle size, needle insertion site, and cerebrospinal fluid (CSF) volume col

77 nation of the intercondylar roof, ACL-tibial insertion site, and PCL angle and horizontal component-t

78 c mutation results from a deletion at the Ds insertion site, and the molecular identity of GLC is not

79 ndicating that the transgene promoter and/or insertion site are critical for sustained expression.

80 Insertion sites are compared among individuals of suitab

81 In the analysis of these screens, transposon insertion sites are typically identified by targeted DNA

82 Thirty-seven per cent (9754) of these insertion sites are within genes (including untranslated

83 We identified three resulting insertion sites (arrayed binding sites), the longest of

84 hrough the pre-insertion, insertion and post-insertion sites as BF alternates between open and closed

85 insert into sites other than its known TTAA insertion site at a low frequency (2%).

86 increase the toughness of the tendon-to-bone insertion site at the expense of its strength.

87 s from lymphomas/leukemias identified common insertion sites at known and candidate novel cancer gene

88 related dsRNA viruses detected preferential insertion sites at which the complexity of the conserved

89 d primary wound repair due to the leakage in insertion sites before the PPV, however remaining 20 cas

90 Therefore, focusing on the insertion site between the anterior cruciate ligament (A

91 Insertion site bias, or lack thereof, has not been demon

92 mation, the template base has moved into the insertion site but misaligns an incorrect nucleotide rel

93 mal translocations associated with the T-DNA insertion site, but the prevalence of these rearrangemen

94 t of a conserved active site tyrosine in the insertion site by the template base is accommodated by a

95 ndonuclease I-AniI and their putative target insertion sites by BLAST searches followed by examinatio

96 pread in two dimensions: horizontally across insertion sites by non-allelic gene conversion, and vert

97 ion in vivo, by mating polB intein-positive [insertion site c in the gene encoding DNA polymerase B (

98 Insertion sites can then be navigated in Ensembl in the

99 and truncated versions of the element in new insertion sites cause stable mutations.

100 nsertions is unclear without high-resolution insertion site characterization, the potential for an ot

101 enables broader intein utility by increasing insertion site choices.

102 Common insertion site (CIS) analysis of 119 primary tumors and

103 Common insertion site (CIS) analysis of 269 neurofibromas and 1

104 eir genomic location is proximal to a common insertion site (CIS) defined by high rates of transposon

105 Analysis of transposon common insertion sites (CIS) identified the Apc locus as a majo

106 ransposons have been used to identify common insertion sites (CISs) associated with tumor formation.

107 nd the genomic location of transposon common insertion sites (CISs) from all currently published tran

108 By analyzing common insertion sites (CISs) isolated from 446 tumors, we iden

109 nts within the genome and 2) identify Common Insertion Sites (CISs) within the genome are not practic

110 ructure, the substrate binds to the active ('insertion') site closed through refolding of the trigger

111 ence and distribution, and (iii) to evaluate insertion site-dependent changes in mechanical propertie

112 nately, the great increase in the numbers of insertion sites detected comes with the cost of not know

113 EpiTEome outperforms other split-read insertion site detection programs, even while functionin

114 han to that of the sea anemone (although the insertion site differs in the fungi).

115 etermine whether genes located at transposon insertion sites directly caused medulloblastomas to diss

116 Insertion site dressings are a major mean to reduce cath

117 erved tyrosine moves into the templating and insertion sites during the translocation step.

118 or some H chain genes could be caused by (i) insertion site effects or (ii) deletion of enhancer elem

119 DNA in regions of FIV insertion sites exhibited a "bendable" structure and a p

120 icient evidence to recommend one CVC type or insertion site; femoral catheterization should be avoide

121 erves relatively undistorted geometry at the insertion site following phosphoryl transfer.

122 ite following transposon excision and at the insertion site following transposon integration.

123 Using this photoprobe, we demonstrated the insertion site for [ (125)I]IACoc to be Asp188, which re

124 and subsequent DNA bending primes a selected insertion site for efficient transposition.

125 specificity data and defining new potential insertion sites for any target.

126 Comparison of insertion sites for IS492 and the highly related ISPtu2

127 equencing technology and is able to identify insertion sites from both the 5' and 3' ends of the tran

128 loped IM-Fusion, an approach that identifies insertion sites from gene-transposon fusions in standard

129 ), that facilitates sequencing of transposon insertion sites from single tumor cells in a SB mouse mo

130 Sequence analysis of transposon insertion sites from tumors and immortalized cells ident

131 The common insertion site genes identified in the mutagenesis scree

132 Both of these insertion sites have previously been mapped to the "clam

133 poson insertion sites and a large portion of insertion sites have zero mapped reads.

134 Analysis of transposon common insertion sites identified 7 genes activated by transpos

135 Isolation of the transposon insertion sites identified genes known to be associated

136 Analysis of transposon insertion sites identified novel candidate genes, includ

137 Cloning DNA flanking the insertion site identifies the locus precisely.

138 a specific double-strand cut near the intron insertion site (IIS), its DNA recognition site spans the

139 ation exhibited lack of deletions at genomic insertion sites, implying that such events are likely to

140 moral site was the most frequently colonized insertion site in all types of catheters.

141 ogy such as fever, carefully inspect the PVC insertion site in bacteremic or fungemic patients, and r

142 sposons form genomic islands at a programmed insertion site in bacterial chromosomes, attTn7.

143 Selection of central venous catheter insertion site in ICU patients could help reduce cathete

144 rtion site in strain MZO-3 and at the VSP-II insertion site in strain 623-39.

145 Novel regions are present at the VSP-I insertion site in strain MZO-3 and at the VSP-II inserti

146 point on the nephric duct (ND) to its final insertion site in the cloaca (the primitive bladder and

147 Using PCR, we identified a transposon insertion site in the first intron of Nmnat2 (Nicotinami

148 ion when the ureters move from their primary insertion site in the Wolffian ducts to the trigone, a m

149 Jak2 was identified as a common transposon insertion site in TLS-ERG-induced disease, strongly vali

150 VL gene sequences, and the chromosomal phage insertion sites in 114 isolates comprising nine clones o

151 mutants with the desired phenotypes, and the insertion sites in 18 of the strains were mapped.

152 s, PVL gene sequences, and chromosomal phage insertion sites in 52 S. aureus CC30 PVL-harboring isola

153 After sequencing transposon insertion sites in 9,250 random mutants, we assembled a

154 e splice finding and elucidation of multiple insertion sites in a random insertional mutagenesis libr

155 es an efficient k-mer-based method to detect insertion sites in a reference genome, and subsequently

156 Pyrosequencing data showed 266 insertion sites in addition to the 325 sites expected fr

157 Sequence analysis of insertion sites in both R. rickettsii and R. prowazekii

158 we used a targeted method to sequence LINE-1 insertion sites in matched PDAC and normal samples.

159 Assessment of insertion sites in peripheral blood from patients in the

160 s in these collections, and (3) validate the insertion sites in pools of mutants by obtaining >500 bp

161 rately corresponded to LINE; retrotransposon insertion sites in ribosomal DNA (p<0.01).

162 us mapping of tens of thousands of mutagenic insertion sites in the eukaryotic unicellular green alga

163 To identify the location of insertion sites in the genome linker based polymerase ch

164 The insertion sites in the genome were pre-N (F1), N-P (F2),

165 Characterization of transposon insertion sites in the SB-induced tumors identified 45 r

166 Analysis of the mPing insertion sites in the soybean genome revealed that feat

167 This increased the number of unique insertion sites in the T-DNA collection by 21 078, bring

168 hods were established to identify transposon insertion sites in these lesions, and analysis of transp

169 were conspicuous among the common transposon insertion sites in TLS-ERG-driven leukemia, suggesting t

170 ic duplications were observed at or near the insertion sites in two patients, further confounding the

171 rvo-controlled actuation of syringeal muscle insertions sites in vitro and focus on two muscles contr

172 ed candidate prostate cancer genes at common insertion sites, including Pde4d.

173 identify retroviral insertions at 19 common insertion sites, including Zeb2, Nf1, Mn1, Evi1, Ift57,

174 However, with this method, the insertion site, integrity, and copy number of the transg

175 Insertion sites interrupting genes that are likely criti

176 We also have determined the optimal insertion site into the NDV genome by generating recombi

177 This approach allows for the clustering of insertion sites into distinct regions of essentiality ac

178 we introduced plasmids containing candidate insertion sites into S. pombe and mapped the positions o

179 Fgfr2(ST-/-) mice exhibit improper ureteral insertion sites into the bladder, consistent with the ur

180 The transgene insertion site is associated with a 12 kb deletion, 1.2

181 f target DNA engagement until an appropriate insertion site is identified.

182 Under the above-stated conditions, insertion site is likely the most contributory variable

183 This study demonstrates that the insertion site is the primary determinant of the respons

184 c and predictive impact of allelic ratio and insertion site (IS) of internal tandem duplications (ITD

185 cus and paired lines in the same chromosomal insertion site lacking the 3' enhancers.

186 tissues suggest that the DNA region near the insertion site likely interacts with Foxl2 TSS.

187 the utility of the method by identifying Mu insertion sites linked to seed-lethal mutations with a p

188 We identified numerous common insertion sites located near protein-coding genes and co

189 Insertion site mapping of cells that survived long-term

190 However, with the lesion in the BF post-insertion site, more serious distortions caused by the a

191 Two common retrovirus insertion sites near c-myb and Sox4 genes were identifie

192 ient population, number of catheters at each insertion site, number of catheter-related bloodstream i

193 eading frames were common, although based on insertion site-occupancy frequency data it appeared that

194 ect to copy number, nucleotide diversity and insertion site-occupancy frequency.

195 Common transposon insertion sites occur among haplotypes from different Ze

196 cer to resolve the structure and chromosomal insertion site of a composite antibiotic resistance isla

197 ied in 14 of the mutants on the basis of the insertion site of a transposable element.

198 e, ECM accurately identified the ventricular insertion site of an accessory atrioventricular connecti

199 Pan proviral insertion site of Moloney murine leukemia (PIM) 1, 2, an

200 The high expression of proviral insertion site of Moloney murine leukemia virus kinases

201 , a systematic study to evaluate the optimal insertion site of the foreign antigens into NDV that res

202 The transposon insertion site of this mutant strain was within Sca2, a

203 nd pathogenic mechanisms of mycoplasmas, the insertion site of transposon Tn4001T was determined for

204 The insertion sites of 17 copies of the G.st.I1 intron from

205 ECGI can noninvasively localize ventricular insertion sites of accessory pathways to guide ablation

206 The insertion sites of femoral catheters were involved in al

207 omparison of the amino acid distributions at insertion sites of introns that retained their positions

208 tirety, the origins, transfer mechanisms and insertion sites of numts are slowly being characterized.

209 :H7 strains were tested for the presence and insertion sites of Shiga toxin gene (stx)-containing bac

210 nd quantify the abundance of > 91,000 unique insertion sites of the provirus from 61 HTLV-1(+) person

211 The insertion sites of the Stx-encoding bacteriophages diffe

212 ximately 2 x 10(5) unique de novo transposon insertion sites of the transposon Hermes in the Saccharo

213 l deletions that were roughly bounded by the insertion sites of the two P elements used.

214 Insertion sites of transposed Ds-ATag elements were iden

215 ing, we devised a technique to determine the insertion sites of virtually all members of the human-sp

216 lving five contiguous genes at the transgene insertion site on chromosome 12C3.

217 The impact of catheter insertion site on infection risk remains controversial.

218 nterior cystic abnormalities at rotator cuff insertion site on the greater tuberosity and to determin

219 A minimum of 15 mtDNA insertion sites on nine chromosomes were detectable usin

220 l approach, we investigated various feasible insertion sites on the G protein of VSV (VSV-G) for disp

221 on of IS492 does not occur at four different insertion sites on the P. atlantica chromosome, despite

222 insertion site) or posterior (infraspinatus insertion site) on the greater tuberosity.

223 Mapping of the insertion sites onto the crystal structure of gB730 sugg

224 less distortion: it is either out of the pre-insertion site or in the major groove open pocket of the

225 positioning itself either deeply in the pre-insertion site or on the crowded evolving minor groove s

226 us cystic changes as anterior (supraspinatus insertion site) or posterior (infraspinatus insertion si

227 lysis and PCR to detect transposable element insertion site polymorphism in a panel of diverse maize

228 Insertion-site preference at the CAN1 locus requires Ty1

229 Our characterization of the insertion site preferences of Hermes not only assists in

230 We have characterized genome-wide insertion site preferences of piggyBac by sequencing a l

231 heir application, we conducted a genome-wide insertion site profiling of the piggyBac (PB), Tol2 and

232 e structural adaptation to insertions is the insertion site rather than the sequence of the insertion

233 utured either 7 mm posterior to its original insertion site (recession surgery) or at the same site (

234 cing allows affordable ultra-deep transposon insertion site recovery in high-throughput formats withi

235 rated that IS5 can precisely excise from its insertion site, restoring the wild-type phenotype.

236 Pol III transcribed genes; alignment of Ty1 insertion sites revealed a strong sequence motif centere

237 ct, high-throughput sequencing of transposon insertion sites revealed fitness phenotypes of a bank of

238 n of the Shiga toxin bacteriophage and their insertion sites (SBI typing) revealed that cattle and sh

239 ethering can effectively redirect transposon insertion site selection in human cells, but suggest tha

240 the R2 ribozyme is strongly modulated by the insertion site sequence in the rDNA, with the most commo

241 Analysis Tool) for the efficient analysis of insertion site sequence reads against vertebrate and inv

242 combinations of endonuclease specificity and insertion site sequences in a biological host.

243 ibe a method for semiquantitative transposon insertion site sequencing (QiSeq).

244 We used transposon insertion site sequencing (Tn-seq) to comprehensively as

245 uman gut epithelial cells was assessed by Tn-insertion site sequencing (Tn-seq).

246 We used transposon-directed insertion site sequencing (TraDIS) to retrospectively an

247 We have used transposon-directed insertion site sequencing (TraDIS) to screen mutants of

248 Using transposon-directed insertion site sequencing (TraDIS), we determined differ

249 we carried out a high-throughput, transposon insertion site sequencing analysis (TnSeq) to identify g

250 present ChlaMmeSeq (Chlamydomonas MmeI-based insertion site Sequencing), a tool for simultaneous mapp

251 nem or ciprofloxacin) by Transposon Directed Insertion-site Sequencing (TraDIS).

252 lly, we demonstrate that transposon-directed insertion-site sequencing is not only applicable to the

253 In this study, we use transposon-directed insertion-site sequencing to probe differences in gene r

254 Transposon insertion-site sequencing was used to analyze the fitnes

255 We here describe quantitative insertion-site sequencing, or QIseq, which uses custom l

256 used genome-wide transposon mutagenesis and insertion-site sequencing, RNA-Seq, plus mass spectromet

257 We use our tagged chromosomal insertion site system to identify small sequences from b

258 number of unique lengths of amplicons of an insertion site tends to increase according to its abunda

259 sease results from a larger number of unique insertion sites than in asymptomatic carriers and not, a

260 fungal donor (perhaps one with an identical insertion site that has not yet been discovered).

261 Of note, we identified a common insertion site that was >100 kb from the nearest coding

262 rates that we have saturated the full set of insertion sites that are actively targeted by Tf1.

263 We identified 43 common proviral insertion sites that contain candidate genes involved in

264 nt and coincided with preferential P-element insertion sites that harbor transcription initiation act

265 alysis of 85 tumors, we identified 77 common insertion sites that map to 56 genes potentially driving

266 nd seven permissive zinc finger domain (ZFD) insertion sites throughout Gag-Pol, including within p12

267 Here, we used a transposon insertion site (TIS) sequencing-based strategy to identi

268 ory sequences, we mapped the H-2Z1 transgene insertion site to chromosome 17, 100 and 460 kb away fro

269 We used information about the transgene insertion site to develop a polymerase chain reaction ge

270 t that it is able to disseminate from a skin insertion site to infect multiple organs.

271 disrupts open reading frames and allows the insertion sites to be readily identified.

272 Global, regional, and local insertion site trends were examined.

273 ely sequenced rice genome to determine 1,664 insertion sites using high-throughput sequencing of 24 i

274 The ACL-tibial insertion site was constant at the junction of the anter

275 The transposon insertion site was identified for 29 of the 150 mutants

276 Vein diameter at the PICC insertion site was measured using ultrasound with in-bui

277 trasound, a radiologist's marking the needle insertion site was not associated with decreased pneumot

278 g MreB filaments are needed to coordinate PG insertion sites, we find that local coordination of enzy

279 city of RET2 and the purine-rich nature of U insertion sites, we propose that the distributive +1 ins

280 no acids that immediately flanked the intein insertion site were randomized.

281 Similar chromosomal phage insertion sites were also identified in all 52 PVL-harbo

282 Ty1 insertion sites were also mapped in four mutant lines th

283 ated with a variety of genomic features: (1) Insertion sites were correlated with regular nucleosome

284 Tumor insertion sites were enriched in recurrent somatic copy-

285 tropy reductions near microdialysis catheter insertion sites were highly correlated with reductions i

286 ransposon mutagenesis screen in which common insertion sites were identified in tumors that were prod

287 rocytoma tissue was extracted and transposon insertion sites were identified.

288 As validation, several insertion sites were introduced into a wild-type clone,

289 Four of these insertion sites were not included in the solved structur

290 Large pools of selected insertion sites were sequenced in a high throughput mann

291 femoral vein (in a 1:1:1 ratio if all three insertion sites were suitable [three-choice scheme] and

292 , showing that they all bind in a common pre-insertion site where the phosphate groups are not yet po

293 le until the replication fork arrives at the insertion site, whereupon the FD is rapidly degraded.

294 mutant phenotype represent candidate linked insertion sites, which are then confirmed by PCR.

295 on, in particular attenuating the effects of insertions sites, which can result in variations in phen

296 Furthermore, by combining the identified insertion sites with expression quantification, we show

297 tational pipeline, TIPseqHunter, to identify insertion sites with high precision and reliability.

298 in (NP)-specific artificial microRNA from an insertion site within the non-structural (NS) gene segme

299 gamma-globin intron by optimizing the intron insertion site within the reporter gene.

300 ls revealed significantly less clustering of insertion sites within LMO2, MECOM, and other lymphoid p