ライフサイエンスコーパス: insertional

1 did not find evidence that AAV vectors cause insertional activation of oncogenes and subsequent tumor

2 transfer using gammaretroviral vectors, but insertional activation of proto-oncogenes contributed to

3 quisition of additional mutations, including insertional activation of Pu.1, resulting in full leukem

4 islands, thereby reducing the potential for insertional activation.

5 s we quantitatively recapitulate patterns of insertional activity that are usually described in quali

6 Furthermore, a transfer (T)-DNA insertional alg3-T2 mutation, which causes accumulation

7 essential for myelopoiesis, we identified an insertional allele hi1727, which disrupts the gene encod

8 A T-DNA insertional allele of the single NEDD1 gene was isolated

9 ith roles in auxin response, we screened mpk insertional alleles and identified mpk1-1 as a mutant th

10 MAS2 is a single-copy gene whose insertional alleles are embryonic lethal.

11 Transfer DNA insertional alleles that completely eliminate the accumu

12 The identities of mur5 and two SALK insertional alleles were confirmed by allelism tests and

13 force and the relative contributions of the insertional and appositional components, however, are un

14 sis to obtain the relative magnitudes of the insertional and appositional contributions to the rib di

15 o simulate, respectively, the effects of the insertional and appositional forces, and the rib traject

16 y of these retroelements commonly results in insertional and post-insertional mechanisms of genomic i

17 A T-DNA insertional atos9-t mutation blocks the degradation of a

18 o the human reference, we find a significant insertional bias (3:1) in regions corresponding to compl

19 ecific effect of foamy virus, independent of insertional bias, contributing to reduced genotoxicity.

20 dentify genes required for a functional CCM, insertional Bleomycin resistance (Ble(R)) mutants were g

21 The generation of a zebrafish insertional cardiac mutant collection shall facilitate t

22 tate cancers can be generated either through insertional chromosomal rearrangement or by intrachromos

23 The editing can be insertional, deletional, or substitutional and has been

24 Analysis of a previously identified insertional dhx16 mutation shows that the zygotic compon

25 The null allele e resulted from an insertional disruption in the CDS by Sal-PIF, a 3100-bp

26 Insertional disruption of one target locus, IPK1, result

27 The insertional disruption of the CD1349 gene resulted in si

28 that were lacking or deficient in GlcCer by insertional disruption or by RNA interference (RNAi) sup

29 Deletion or insertional duplication of an evolutionarily conserved r

30 Insertional dysploidy has been recorded in three grass s

31 The existence of insertional editing makes gene identification difficult

32 ted at a significantly higher frequency than insertional editing sites and that substitutional editin

33 y individual polymorphisms, depending on the insertional environment within the host genome.

34 Taken together, these results indicate that insertional events should be considered as alternatives

35 ory effect of pleural pressure, and that the insertional force contributes 60% of that inspiratory ef

36 e fibres on the ribs into which they insert (insertional force) and the transmission of abdominal pre

37 in which pM92 expression was disrupted by an insertional/frameshift mutation.

38 The T-DNA insertional gain-of-function mutant plant for LTP5 (ltp5

39 es, which led to the isolation of 29 clones (insertional gametocyte-deficient mutants) that fail to f

40 ion of spontaneous tumors with characterized insertional genetic landscapes.

41 t binds CCCTC-binding factor and reduces its insertional genotoxicity.

42 in parallel, strongly suggesting that these insertional inactivation mutations were adaptive.

43 hese have resulted in the direct deletion or insertional inactivation of 13 genes.

44 Insertional inactivation of a gene in this cluster encod

45 Insertional inactivation of a putative integrase gene on

46 Insertional inactivation of the aerobically expressed ni

47 A H. ducreyi luxS mutant was constructed by insertional inactivation of the luxS gene and lost the a

48 gene encoding an IFN-gamma binding protein, insertional inactivation of the thymidine kinase gene, a

49 We next isolated and characterized insertional knockout mutants of all three isoforms confi

50 However, homozygous insertional knockout mutations or RNAi knock-down constr

51 In the nhx5 nhx6 T-DNA insertional knockouts, the precursors of the 2S albumin

52 Mutants with an insertional lesion in the bslO gene exhibit exaggerated

53 Here we show that an insertional lesion in the sap structural gene results in

54 cis chain length, we identified mutants with insertional lesions in secA2.

55 d by the analysis of the corresponding T-DNA insertional lines.

56 nferring resistance, we observed somatic and insertional loss-of-function mutations in transformation

57 nts commonly results in insertional and post-insertional mechanisms of genomic injury.

58 We show this insertional method generates mutant alleles that are rev

59 The combination of this reporter-tagged insertional mutagen approach and zebrafish provides a po

60 icotiana tabacum) Tnt1 retrotransposon as an insertional mutagen in potato.

61 efficient use of lentiviral validation-based insertional mutagenesis (VBIM) to generate large populat

62 Using validation-based insertional mutagenesis (VBIM), we identified family wit

63 endogenous L1s suggests that it is unlikely insertional mutagenesis alone accounts for the Mov10l1(-

64 esis, we generated Cfp4-deficient strains by insertional mutagenesis and by RNA interference.

65 This can lead to genetic damage through insertional mutagenesis and chromosomal rearrangements b

66 hough powerful enhancer elements have caused insertional mutagenesis and clonal dysregulation.

67 g concerns related to persistent expression, insertional mutagenesis and cytotoxicity.

68 experimentally profile Cas9 using randomized insertional mutagenesis and delineate hotspots in the st

69 Insertional mutagenesis and depletion (iMAD) is a geneti

70 eveloped a genetic screening strategy called insertional mutagenesis and depletion (iMAD) that combin

71 e families (L1, Alu and SVA) associated with insertional mutagenesis and disease.

72 tegrating vectors pose risks associated with insertional mutagenesis and disruption of gene expressio

73 L1 can introduce genomic instability via insertional mutagenesis and DNA double-strand breaks, bo

74 r suppressor using mouse transposon-mediated insertional mutagenesis and Drosophila cancer models.

75 red potentially hazardous, as it can lead to insertional mutagenesis and genomic instability.

76 Insertional mutagenesis and genotoxicity, which usually

77 al dominance that could not be attributed to insertional mutagenesis and instead likely resulted from

78 n, a method that is associated with unwanted insertional mutagenesis and positional effects on transg

79 r, their clinical use raises the concerns of insertional mutagenesis and potential oncogenesis due to

80 etrotransposon can damage the genome through insertional mutagenesis and the generation of DNA double

81 for transposon-mediated genome engineering, insertional mutagenesis and the genome-wide mapping of t

82 , association analysis, expression analysis, insertional mutagenesis and transgenic validation, we de

83 integration into the host genome results in insertional mutagenesis and variable position effects.

84 method for gene therapy because the risks of insertional mutagenesis are eliminated provided that a "

85 acity, host immune response, and the risk of insertional mutagenesis are limiting factors and of conc

86 ha(-/-) and DAGLbeta(-/-) mice generated via insertional mutagenesis by gene-trapping with retroviral

87 Insertional mutagenesis by retroviral vectors is a major

88 Here, we show that insertional mutagenesis by the PiggyBac transposon can b

89 Together, these results indicate that SB-insertional mutagenesis can identify high-grade astrocyt

90 Genome-wide functional profiling by insertional mutagenesis can reveal protein domains essen

91 g capacity, lack of copy number control, and insertional mutagenesis caused by integration into host

92 correction of monogenic diseases without the insertional mutagenesis caused by multisite integration

93 Although L1-induced insertional mutagenesis causes Mendelian disease, their

94 allenge that remains is reducing the risk of insertional mutagenesis due to random insertion by both

95 Using insertional mutagenesis each gene was characterized and

96 ac transposon system for tamoxifen inducible insertional mutagenesis from a stably integrated chromos

97 d donors, leukemia caused by vector-mediated insertional mutagenesis has been reported in some indivi

98 Malignant outcomes from insertional mutagenesis have featured prominently in the

99 Insertional mutagenesis in a haploid background can disr

100 complicated by acute leukemia as a result of insertional mutagenesis in a high proportion of patients

101 ates tumorigenesis following Sleeping Beauty insertional mutagenesis in a mouse model of melanoma.

102 use Sleeping Beauty (SB) transposon-mediated insertional mutagenesis in a mouse model of pancreatic d

103 We used retroviral insertional mutagenesis in an effort to identify which g

104 tudy describes a tool, Lentihop, for somatic insertional mutagenesis in human cells and uses this sys

105 ee) iPSCs, which avoid the potential risk of insertional mutagenesis in humans.

106 oid leukemias (AMLs) generated by retroviral insertional mutagenesis in Kras(G12D) "knockin" mice wit

107 deficiency, the UCE gene was inactivated via insertional mutagenesis in mice.

108 genous MLL-AF9 and MLL-ENL oncogenes through insertional mutagenesis in primary human hematopoietic s

109 m that can be used for effective large-scale insertional mutagenesis in soybean.

110 Sleeping Beauty insertional mutagenesis in this model led to accelerated

111 Insertional mutagenesis is a cornerstone of functional g

112 Gene-trap insertional mutagenesis is a high-throughput forward gen

113 Insertional mutagenesis is a powerful tool for determini

114 Retroviral insertional mutagenesis is a powerful tool for identifyi

115 ate near genes and regulatory regions; thus, insertional mutagenesis is a substantial risk.

116 However, in some patients, insertional mutagenesis led to leukemia or myelodysplasi

117 red in HYDA1 gene expression by screening an insertional mutagenesis library for HYDA1 promoter activ

118 r results demonstrate that extensive somatic insertional mutagenesis occurs very early during the dev

119 RISPR-Cas9 reverse-genetics pipeline enabled insertional mutagenesis of 18 of these 20 transcription

120 ilization likely promotes tumor formation by insertional mutagenesis of cancer genes, and not by prom

121 Insertional mutagenesis of exsM in Bacillus anthracis De

122 Insertional mutagenesis of legume genomes such as soybea

123 Insertional mutagenesis of regions upstream of the chara

124 Second, the H-2Z1 transgene causes insertional mutagenesis of Tbc1d5 and Satb1, leading to

125 "complex interaction" model was tested with insertional mutagenesis of the Drosophila Hsp70 core pro

126 We show that insertional mutagenesis of the previously uncharacterize

127 and tractable manner, using transposon-based insertional mutagenesis on the background of chronic pha

128 of the encoded CPS and KS, and the impact of insertional mutagenesis on virulence and the plant defen

129 ted in single-gene disease in humans through insertional mutagenesis or aberrant mRNA splicing.

130 Insertional mutagenesis or RNA-mediated silencing of Os-

131 use of viral vectors prone to silencing and insertional mutagenesis or the use of nonhuman genes.

132 cancers in mice using a transposon-mediated insertional mutagenesis protocol.

133 Disruption of chiA by insertional mutagenesis resulted in cells that failed to

134 r MPXV infection, we performed a genome-wide insertional mutagenesis screen in human haploid cells.

135 Here we developed a novel whole-body insertional mutagenesis screen in mice, which was design

136 ducted a Sleeping Beauty transposon-mediated insertional mutagenesis screen in Rassf1a-null mice to i

137 ansform mammary epithelial cells, we used an insertional mutagenesis screen on cells isolated from wi

138 67 stable transgenic lines generated from an insertional mutagenesis screen using a transposon-based

139 To identify these genes, we performed an insertional mutagenesis screen using the Sleeping Beauty

140 An insertional mutagenesis screen was performed by intercro

141 Using a gene-trap insertional mutagenesis screen, we identified poliovirus

142 In a large-scale zebrafish insertional mutagenesis screen, we identified the pinbal

143 ets of new cancer genes through a pancreatic insertional mutagenesis screen.

144 f rapamycin (ztor) mutant identified from an insertional mutagenesis screen.

145 recent major advances in transposon-mediated insertional mutagenesis screens and compare this technol

146 Yet, retroviral insertional mutagenesis screens identify RUNX genes as c

147 elements, have been successfully applied for insertional mutagenesis screens in both the mouse and ra

148 nal annotation of cancer genomes by enabling insertional mutagenesis screens in higher eukaryotes tha

149 Insertional mutagenesis screens in mice are used to iden

150 Insertional mutagenesis screens play an integral part in

151 t to identify DNA integration sites, such as insertional mutagenesis screens, gene and enhancer trap

152 cell proliferative capacity without risk of insertional mutagenesis should have broad utility in dis

153 We demonstrate that the insertional mutagenesis system based on Tnt1 and the 523

154 id piggyBac/Sleeping Beauty transposon-based insertional mutagenesis system that can be mobilized by

155 dology with a tight integration of gene-trap insertional mutagenesis testing and systems biology to i

156 cribe here a large series of mouse lines for insertional mutagenesis that are compatible with two tra

157 that these vectors are an effective tool for insertional mutagenesis that can be used for either gene

158 selection and also explain the high risk of insertional mutagenesis that is associated with gene the

159 Here we used piggyBac (PB) transposon insertional mutagenesis to anticipate resistance mechani

160 We used insertional mutagenesis to develop a screening method to

161 Here we combined retroviral and transposon insertional mutagenesis to enable cancer gene discovery

162 e unicellular green alga Chlamydomonas using insertional mutagenesis to find mutants that conferred h

163 In this study, we used retroviral insertional mutagenesis to identify genes that accelerat

164 n in Chinese hamster ovary (CHO) cells using insertional mutagenesis to identify genes that regulate

165 used genome-wide CRISPR libraries as well as insertional mutagenesis to identify synthetic viable (ge

166 used an unbiased method for validation-based insertional mutagenesis to isolate a quinacrine-resistan

167 Here, we recruited retroviral insertional mutagenesis to obtain induction of an arbitr

168 sposase alleles to allow transposon-mediated insertional mutagenesis to occur.

169 ned the assay with Agrobacterium tumefaciens insertional mutagenesis to screen for hyphal mutants.

170 y (SB) transposon system has been used as an insertional mutagenesis tool to identify novel cancer ge

171 Insertional mutagenesis using engineered transposons is

172 w drivers of intestinal cancer, we performed insertional mutagenesis using the Sleeping Beauty transp

173 Insertional mutagenesis using transfer DNA or transposab

174 6a12 hypomorphic rat generated by transposon insertional mutagenesis was characterized using RT-PCR,

175 Here, transposon-mediated insertional mutagenesis was used to create 5 libraries o

176 te cellular promoters pose a reduced risk of insertional mutagenesis when compared with vectors with

177 study provides a new mechanism of retrovirus insertional mutagenesis whereby spatial chromatin organi

178 could be modified to drive transposon-based insertional mutagenesis wherever tissue-specific Cre exp

179 We performed insertional mutagenesis with the MOL4070LTR retrovirus i

180 ful tools for creating transgenic organisms, insertional mutagenesis, and genome engineering.

181 ta suggest that ALV-J induces oncogenesis by insertional mutagenesis, and integrations in the MET onc

182 y-one gene studies using positional cloning, insertional mutagenesis, antisense morpholino oligonucle

183 nt populations on par with those produced by insertional mutagenesis, but systematic cataloguing of m

184 with the cellular receptors, we implemented insertional mutagenesis, carbohydrate shielding, and ala

185 etic manipulation including genetic crosses, insertional mutagenesis, chemical mutagenesis, homologou

186 hroughout the genome, potentially leading to insertional mutagenesis, inappropriate activation of nea

187 To deal with potential insertional mutagenesis, the vector integrations are the

188 Owing to the risk of insertional mutagenesis, viral transduction has been inc

189 Using piggyBac transposon-mediated insertional mutagenesis, we screened for parasites that

190 erapy strategies, e.g., immune rejection and insertional mutagenesis, which are associated with viral

191 ion of gene expression - carries the risk of insertional mutagenesis, which can lead to activation of

192 somatic mosaicism during neurodevelopment by insertional mutagenesis.

193 ld pose a risk for neoplasia, independent of insertional mutagenesis.

194 ing data for 97 tumors induced by retroviral insertional mutagenesis.

195 -causing mutations and eliminate the risk of insertional mutagenesis.

196 coding gene cluster, which was identified by insertional mutagenesis.

197 d catalog of common and rare variants due to insertional mutagenesis.

198 MKL mouse model generated through retroviral insertional mutagenesis.

199 erating mutations were induced by retroviral insertional mutagenesis.

200 ding the putative adhesins were disrupted by insertional mutagenesis.

201 a (SREBP-1a) was specifically inactivated by insertional mutagenesis.

202 ro and in vivo with any organism amenable to insertional mutagenesis.

203 episome in replicating cells while avoiding insertional mutagenesis.

204 viding cells, exhibiting a decreased risk of insertional mutagenesis.

205 screen of mutant mice generated by piggyBac insertional mutagenesis.

206 olet (UV) light and chemical mutagens, or by insertional mutagenesis.

207 for therapeutic application due to potential insertional mutagenesis.

208 ant near-haploid KBM-7 cells using gene-trap insertional mutagenesis.

209 Here, we performed a large-scale insertional mutagenic screen of injury-resistant podocyt

210 Retrotransposons are "copy-and-paste" insertional mutagens that substantially contribute to ma

211 The use of transposons as insertional mutagens to identify cancer genes in mice ha

212 Of these five, only insertional mutant alleles of RGP2, a gene that encodes

213 ome position of the flanking sequence of the insertional mutant CAL027_01_20 was used to identify the

214 An axy9 insertional mutant exhibits severe growth defects and co

215 f a million flanking sequence tags from rice insertional mutant libraries are publicly available.

216 Screening of a Chlamydomonas insertional mutant library identified a strain strongly

217 a-3 fatty acids was isolated by screening an insertional mutant library using gas chromatography-base

218 a screen for liver defects from a zebrafish insertional mutant library, we isolated the mutant cdipt

219 osable Element from Nicotiana tabacum (Tnt1) insertional mutant line were carried out in the one M. t

220 Three independent T-DNA insertional mutant lines deficient in 6-phosphofructo-2-

221 In this study, two independent transfer DNA insertional mutant lines of tim17-1 exhibited a germinat

222 Like the th2-1 mutant, an insertional mutant of At5g32470 accumulated ThMP, and th

223 We report that a commonly used insertional mutant of FoxP3 (GFP-Foxp3) modified its mol

224 The RNA interference lines and the T-DNA insertional mutant of OsbZIP48 showed seedling-lethal ph

225 Finally, we present a new mouse insertional mutant of Oxr1, confirming that specific dis

226 In this study, we identified an insertional mutant of the wall teichoic acid (WTA) synth

227 Altering Gle1 using an insertional mutant or antisense morpholinos results in m

228 NA tagged transformants from the Taiwan Rice Insertional Mutant resource.

229 Surprisingly, the bin2bil1bil2 triple T-DNA insertional mutant still responds to BR and accumulates

230 terization of pCRY was performed based on an insertional mutant that expresses only 11% of the wild-t

231 We generated an analogous insertional mutant to confirm the phenotype and characte

232 An insertional mutant, nrr1-1, accumulates only 50% of the

233 pression is significantly reduced in an acry insertional mutant.

234 ing early stages of development in the T-DNA insertional mutant.

235 In this study, we isolated AGO2 and AGO3 insertional mutants and confirmed that AGO3 is more abun

236 By examining gene expression patterns, insertional mutants and cross-species complementation st

237 C. reinhardtii insertional mutants defective in BBS1, -4, and -7 assemb

238 Our analysis of drp2 insertional mutants demonstrates that, similar to the DR

239 Similar to mpk1-1 and mkk3-1 mutants, rbk1 insertional mutants display auxin hypersensitivity, cons

240 Rice researchers make extensive use of insertional mutants for the study of gene function.

241 We present a new tool with which to produce insertional mutants in P. patens in a rapid and straight

242 ed in IRT1 degradation, we screened a set of insertional mutants in RING-type E3 ligases and identifi

243 protein trap gene-breaking transposon (GBT) insertional mutants in the zebrafish, Danio rerio.

244 ZInC is a web-searchable interface of insertional mutants in zebrafish.

245 Through examining all available T-DNA insertional mutants of Arabidopsis MCTPs, we further rev

246 Transfer DNA insertional mutants of CML38 show an enhanced sensitivit

247 al targets for new therapeutics, we screened insertional mutants of T. gondii for a reduced ability t

248 e, we report characterization of three T-DNA insertional mutants of the gene encoding cytochrome b5 r

249 We identified 14 independent T. gondii insertional mutants out of >8000 screened that share a d

250 Analysis of insertional mutants revealed that cytosolic NADP-ME2 is

251 on and activation model to identify parasite insertional mutants that have a fitness defect in infect

252 he large-scale generation of a population of insertional mutants that have been screened for phenotyp

253 the largest phenotyped collections of algal insertional mutants to date.

254 Xyloglucan from bgal10 insertional mutants was found to contain a large proport

255 re, it was observed that aniA, ccp, and norB insertional mutants were attenuated for biofilm formatio

256 In this study, insertional mutants were created and screened to identif

257 Targeted deletion and insertional mutants were generated in different GBS clin

258 Insertional mutants, pdat1-1 and pdat1-2, accumulate 25%

259 insertions and make it easier to select for insertional mutants, we have developed a two-component s

260 er-represented by sixfold among the screened insertional mutants, with insertion events isolated in o

261 e suppression, RNAi, and characterization of insertional mutants.

262 hondrial enzymes in valine degradation using insertional mutants.

263 hich the paramutant allele was induced by an insertional mutation and displayed the "white-tail-tip"

264 loss-of-function bin2 alleles and one T-DNA insertional mutation each for BIN2 and its two closest h

265 e express PrP that harbor a nine-octapeptide insertional mutation homologous to one described in a fa

266 Craniofacial defects in mice carrying an insertional mutation in Dlgh-1 suggest that Dlgh-1 is re

267 cits seen in mutants lacking TBs; and 2) the insertional mutation in shk mice is sufficient to produc

268 A transfer-DNA insertional mutation in the cdkg1 gene leads to a temper

269 We used ClosTron to construct an insertional mutation in the dnaK gene of C. difficile 63

270 The 39-amino acid insertional mutation in the Rv1-AR (E3DM-AR) sensitizes

271 Insertional mutation of the pqrABCR genes resulted in hy

272 the future, ZInC may include data from other insertional mutation projects as well.

273 antisense morpholino oligonucleotides and an insertional mutation to inhibit its function during earl

274 An insertional mutation within the LTO1 promoter is associa

275 Among these loci, insertional mutations in aspA, aspB, and sodB resulted i

276 henotypes of C. difficile strains containing insertional mutations in either cwp84 or its paralog cwp

277 rpose of this study was to determine whether insertional mutations in gB had differential effects on

278 We found that insertional mutations near the N terminus and C terminus

279 Insertional mutations resulted in embryo lethality that

280 In contrast, transfer DNA insertional null allele of the CAC1A gene is embryo leth

281 Insertional oncogene activation and aberrant splicing ha

282 in limb development, and represent the first insertional phenotype to be cloned in amphibians.

283 of the transduction apparatus, tip links and insertional plaques.

284 emonstrated that they show various levels of insertional polymorphism in mule deer individuals.

285 Thus, P-MLV ERVs show more insertional polymorphism than X-MLVs, and these differen

286 This identified numerous novel human L1(Ta) insertional polymorphisms with highly variant allelic fr

287 These observations suggest that distinct insertional preferences were primary factors that result

288 Beyond revealing interesting properties of insertional RNA editing in Myxomycetes, our work suggest

289 y, we show that the codon position bias in C insertional RNA editing of these two organisms is correl

290 essential genes using a transposon-directed insertional sequencing (TraDIS) approach.

291 not explained solely by foamy virus' modest insertional site preference for nongenic regions compare

292 Analysis of an insertional T-DNA mutant within the REME1 coding sequenc

293 Insertional therapies have shown great potential for com

294 tructural genes showed the highest degree of insertional tolerance.

295 ed light-harvesting antenna size3 (tla3) DNA insertional transformant of Chlamydomonas reinhardtii is

296 ations (8.4%), adjacent duplications (4.2%), insertional translocations (2.5%), or other complex rear

297 uch as triplications, inverted duplications, insertional translocations, and chromothripsis.

298 An insertional transposon mutation in the sll0606 gene was

299 By testing insertional variants in genes for cell wall-anchored sur

300 os (AGM) region, we screened a collection of insertional zebrafish mutant lines for expression of the