ライフサイエンスコーパス: integrant

1 arent; the other parent does not contain the integrant.

2 printed lines had a single 15.7-kb transgene integrant.

3 onal envelope proteins are supplied from HBV integrants.

4 rces and can be stably maintained as genomic integrants.

5 n sites was developed and used to clone 1029 integrants.

6 cB gene that allows for the resolution of co-integrants.

7 no influence on promoter activity in stable integrants.

8 sgene rearrangement in single-copy transgene integrants.

9 RNA gene, permitting biological selection of integrants.

10 ndomized position in sequenced concerted DNA integrants, although in some instances there were prefer

11 s method relied on in vivo selection of rAAV integrants and could be employed for the liver but not f

12 These integrants appear to be maintained by cellular prolifera

13 Moreover, many integrants are defective, and new studies are required t

14 Individual integrants are isolated from bacteria containing drug-re

15 High yields of targeted integrants are obtained, even in a recA background.

16 anded breaks (DSB) but the resulting nuclear integrants are often immediately unstable.

17 Integrants are selected as antibiotic-resistant transfor

18 Following integration, integrants are stably maintained in the absence of antib

19 L. monocytogenes, forms stable, single-copy integrants at a frequency of approximately 10(-4) per do

20 modest effect on expression of some proviral integrants at late times post-transduction.

21 which can be used as a marker for selecting integrants at mutant ade2 loci commonly present in labor

22 The markedly more integrant BBB in neonatal brain than in adult brain afte

23 SV and TVCV, one of the parents contains the integrant but is has not been seen to be activated in th

24 lines, Hep3B and PLC/PRF/5, that contain HBV integrants but do not produce HBV virions and have no si

25 or contains the KanMX selectable marker, and integrants can be selected by resistance to G418.

26 or contains the hisG-URA3-hisG cassette, and integrants can be selected by uracil prototrophy.

27 integration junctions using high-throughput integrant capture sequencing of infected human cells.

28 Using integrant capture sequencing, more than 600,000 AAV-5 in

29 Using a novel high-throughput approach, integrant capture sequencing, nearly 12 million AAV junc

30 New integrants carry all structural characteristics typical

31 Molecular characterization of the mutant ITR integrants confirmed the presence of the trs mutation in

32 Moreover, the number of random integrants decreased by over 54-fold resulting in a 1:3

33 Over time, phenotypic analysis of clonal integrants demonstrated that SB undergoes additional pos

34 The double integrant efficiently incorporates 3H-thymidine into nuc

35 All characterized chloroplast integrants exist apart from native mitochondrial genes,

36 Whole-genome sequencing revealed HPV integrants flanking and bridging extensive host genomic

37 h-throughput creation of libraries of random integrants for purposes including gene knock-out librari

38 Indeed, integrants for three of the segments were not obtained i

39 DNA integrant frequencies were stable over time (<4-fold dif

40 esult in significant reductions in recovered integrants from bacteria, due to increases in one-ended

41 Among the recovered integrants from reactions with mutated U5 but not U3 IN

42 The viral early gene promoter in one integrant gave rise to an unspliced fusion transcript co

43 Five site-specific adeno-associated virus integrants generated in a model system with an Epstein-B

44 The functional properties of the integrant-generated HBsAgs were examined using two human

45 Many integrants have been sequenced, and have all of the hall

46 Among the site-specific integrants, however, a third experienced gene silencing.

47 tal ADA enzyme activity and ADA activity per integrant in the transduced cells demonstrated that the

48 t is not remobilized producing stable clonal integrants in all daughter cells.

49 The results show the absence of EAV and ALV integrants in DNA prepared from MVVE-inoculated human ce

50 omoter activity induced by culture of stable integrants in high glucose.

51 ands of endogenous retrovirus (ERV) germline integrants in highly divergent, previously unsequenced m

52 Determining the diversity of host genes with integrants in HIV-infected cells that persist for prolon

53 ent methods, we were unable to identify rAAV integrants in mouse muscle.

54 ite analysis revealed common proviral vector integrants in NOD/SCID repopulating cells and in the bab

55 of DSB formation in a single-copy minilocus integrant indicates that efficient DSB formation at the

56 The structure of the integrants is complex, and it is thought that episomal v

57 Each integrant line chosen for analysis harbors a single copy

58 The number of integrant loci is low for BSV and PVCV and high in TVCV.

59 We present a model of "looping" by which HPV integrant-mediated DNA replication and recombination may

60 by mouse mammary tumor virus (MMTV) proviral integrant Mtv-1, we have analyzed these regions in T cel

61 eletion of chromosomal DNA, and another DHBV integrant occurred in a telomeric repeat sequence.

62 In five lines of mice carrying multicopy integrants of constructs that either lacked Edelta or ca

63 Resulting targeted single-copy integrants of the p53-CAT reporter show strong genotoxic

64 Integrants of three pararetroviruses, Banana streak viru

65 omoter, allowing identification of potential integrants on the basis of phenotype.

66 HIV-1 provirus, either as a chromosomal integrant or as an episomal plasmid in HeLa cells, forms

67 expressed in R. rubrum either as single-copy integrants or on multiple-copy plasmids, these variants

68 with gene-trap virus produced from a single-integrant packaging cell line that allowed us to quantit

69 s maintained a relatively constant HIV-1 DNA integrant pool that was genetically stable during long-t

70 Both of these integrants provide further evidence that concerted integ

71 underpin clonal selection of one particular integrant remain poorly understood.

72 tion of the products is consistent with most integrants representing a concerted integration in which

73 id was random except that the orientation of integrants selected was apparently influenced by supF tr

74 Plasmid integrants selected with mevinolin were resolved with 5-

75 All of the integrants sequenced were inserted into different sites

76 In 2 of 14 mutant integrants sequenced, deoxynucleotides were deleted from

77 In a striking number of integrants, short identical sequences were shared betwee

78 Sequence analyses of individual HIV-1 integrants showed loss of 2 bp from the ends of the dono

79 An analysis of numerous clonal proviral integrants showed that mutation of CpGs in both clusters

80 he target cassette, we were able to identify integrants simply by the loss of mini-white eye color.

81 Subsequently, this integrant strain is transformed with a plasmid expressin

82 extant Tf2, and silence and immobilize a Tf1 integrant that becomes sequestered into Tf bodies.

83 of CpGs in both clusters eliminated proviral integrants that were completely silenced.

84 iently increased promoter activity in stable integrants, the increase was not sustained (6 h).

85 There are two forms of integrant, those that can form episomal viral infections

86 One DHBV integrant was associated with a small deletion of chromo

87 The integrant was unable to process the trnD transcript at t

88 GFP expression from transposase-mediated integrants was Mendelian through the eighth generation.

89 gration sites, and GFP expression from these integrants was stable over time.

90 alactosidase expression of a pyr-lacZ fusion-integrant were isolated as blue colonies on X-Gal (5-bro

91 truncated L1s, as over 52% (27/51) of total integrants were <1/3 the length of a full-length element

92 ntegrated AAV indicated that essentially all integrants were AAVS1 site specific.

93 several positions randomized, the concerted integrants were examined for statistically significant r

94 Forty-two de novo integrants were recovered that faithfully mimic many asp

95 onjugal transfer into P. aeruginosa, plasmid integrants were selected, and deletion of unwanted DNA s

96 cellular DNA segments that flanked proviral integrants were sequenced to confirm identity.

97 s well as individual clones harboring single integrants--were analyzed for reporter expression during

98 onstruct targeting DNMT1 and isolated stable integrants with different levels of protein.

99 s the first detailed analysis of baculovirus integrants within mammalian cells.

100 Mu; target-primed replication of the linear integrant would subsequently break the chromosome.

101 One of the seven intein integrants yielded I-TevI of high activity.