ライフサイエンスコーパス: integrin alphaIIbbeta3

1 a integrin alpha5beta1), and fibrinogen (via integrin alphaIIbbeta3).

2 ities in a major platelet adhesion receptor, integrin alphaIIbbeta3.

3 that talin binding is sufficient to activate integrin alphaIIbbeta3.

4 C45 interaction with the cytoplasmic face of integrin alphaIIbbeta3.

5 IX-V activate the ligand-binding function of integrin alphaIIbbeta3.

6 ppears to be a key regulator of the platelet integrin alphaIIbbeta3.

7 SLP-76 is involved in signaling mediated by integrin alphaIIbbeta3.

8 acellular signaling leading to activation of integrin alphaIIbbeta3.

9 and disrupted its physical association with integrin alphaIIbbeta3.

10 In addition, mSpeB2 bound purified platelet integrin alphaIIbbeta3.

11 data had been generated with RGD binding to integrin alphaIIbbeta3.

12 ement of the platelet/megakaryocyte-specific integrin alphaIIbbeta3.

13 t CIB is a candidate regulatory molecule for integrin alphaIIbbeta3.

14 e for their specific binding to the platelet integrin alphaIIbbeta3.

15 in a loss of the ligand binding function of integrin alphaIIbbeta3.

16 b molecules, AP7 and PAC1.1, to the platelet integrin alphaIIbbeta3.

17 platelet adhesion to IgG independent of the integrin alphaIIbbeta3.

18 bunits promote constitutive signaling by the integrin alphaIIbbeta3.

19 on platelet activation by GPVI, CLEC-2, and integrin alphaIIbbeta3.

20 activation, and subsequent activation of the integrin alphaIIbbeta3.

21 ted platelet disorder caused by mutations in integrin alphaIIbbeta3.

22 GD peptides and the extracellular domains of integrin alphaIIbbeta3.

23 ranule protein retention, and lack of active integrin alphaIIbbeta3.

24 eta3 cytosolic tail causes activation of the integrin alphaIIbbeta3.

25 side and blocked talin-induced activation of integrin alphaIIbbeta3.

26 aIIb subunit of the highly abundant platelet integrin alphaIIbbeta3.

27 d into integrin antagonists for the platelet integrin alphaIIbbeta3.

28 stimulation activates platelet spreading and integrin alphaIIbbeta3.

29 c example of this phenomenon is the platelet integrin, alphaIIbbeta3.

30 th signaling pathways involving the platelet integrin, alphaIIbbeta3.

31 hanisms are based on studies of the platelet integrin, alphaIIbbeta3.

32 elet agonists increase the affinity state of integrin alphaIIbbeta3, a prerequisite for fibrinogen bi

33 roposed to follow the structural template of integrin alphaIIbbeta3(A711P).

34 s (PMAs), p-selectin expression (P-SEL), and integrin alphaIIbbeta3 activation (PAC-1 binding) were a

35 s directly bind to TLR2 and induces platelet integrin alphaIIbbeta3 activation and P-selectin express

36 PI3K activation regulates platelet integrin alphaIIbbeta3 activation and platelet aggregati

37 ADAP also promotes integrin alphaIIbbeta3 activation in platelets, which la

38 Integrin alphaIIbbeta3 activation is essential for plate

39 intracellular signaling cascades, leading to integrin alphaIIbbeta3 activation, a process antagonized

40 evels of platelet activation, as measured by integrin alphaIIbbeta3 activation, alpha-granule secreti

41 lting in defects in alpha-granule secretion, integrin alphaIIbbeta3 activation, and actin assembly.

42 ses of alpha and dense-granule secretion and integrin alphaIIbbeta3 activation, and aggregation were

43 Platelet aggregation, integrin alphaIIbbeta3 activation, and alpha-granule and

44 and have decreased alpha-granule secretion, integrin alphaIIbbeta3 activation, and protein tyrosine

45 rmsii was capable of triggering platelet and integrin alphaIIbbeta3 activation, as indicated by the e

46 e in GPIb-IX-V function and agonist-mediated integrin alphaIIbbeta3 activation, associated with loss

47 let adhesion to collagen in flow conditions, integrin alphaIIbbeta3 activation, washed platelet secre

48 the molecular mechanisms regulating platelet integrin alphaIIbbeta3 activation.

49 ulation of these cells displayed recovery of integrin alphaIIbbeta3 activation.

50 d was not mediated by glycoprotein IIb/IIIa (integrin alphaIIbbeta3) activation.

51 ssociated with a shear-dependent increase in integrin alphaIIbbeta3 adhesive function, resulting in e

52 lins, kindlins have little primary effect on integrin alphaIIbbeta3 affinity for monovalent ligands a

53 Integrin alphaIIbbeta3 affinity regulation by talin bind

54 Although agonist-induced integrin alphaIIbbeta3 affinity regulation was unaltered

55 ey to fibrinogen (Fg) uptake, trafficking of integrins (alphaIIbbeta3, alphavbeta3), and purinergic r

56 e, Borrelia burgdorferi (sensu lato) bind to integrins alphaIIbbeta3, alphavbeta3 and alpha5beta1 in

57 action was promoted by ligand binding to the integrin alphaIIbbeta3 and by guanosine triphosphate (GT

58 n monoclonal antibodies that are specific to integrin alphaIIbbeta3 and can potently inhibit platelet

59 s strongly inhibited the interaction between integrin alphaIIbbeta3 and fibrinogen and platelet aggre

60 receptor-dependent activation of the surface integrin alphaIIbbeta3 and subsequent binding of fibrino

61 procoagulant platelet-associated changes in integrin alphaIIbbeta3 and the physiologic role of proco

62 vation that involves the binding of MMP-2 to integrin alphaIIbbeta3 and the subsequent cleavage of PA

63 a3 integrin-deficient mice (lacking platelet integrin alphaIIbbeta3 and the widely expressed nonplate

64 lpha2beta1 promoted inside-out activation of integrin alphaIIbbeta3 and thrombus formation.

65 rsCD40L specifically bound to purified integrin alphaIIbbeta3 and to activated platelets in a b

66 ognize the TM helices of two closely related integrins (alphaIIbbeta3 and alphavbeta3) in micelles, b

67 In the case of integrins alphaIIbbeta3 and alpha5beta1, the integrin bi

68 idues in the alpha-subunits of the two beta3 integrins alphaIIbbeta3 and alphaVbeta3, but a potential

69 an attractive candidate bacterial ligand for integrins alphaIIbbeta3 and alphavbeta3.

70 The integrins, alphaIIbbeta3 and alphaVbeta3, play a key rol

71 phaIIbbeta3: the bacterium bound to purified integrin alphaIIbbeta3, and bacterial binding to platele

72 e phosphorylated after fibrinogen binding to integrin alphaIIbbeta3, and collagen-stimulated platelet

73 egation, expression of the activated form of integrin alphaIIbbeta3, and exposure of phosphatidylseri

74 conformational changes in the major platelet integrin, alphaIIbbeta3, and induce minor changes in pla

75 In addition to integrin alphaIIbbeta3, another as-yet-unidentified rece

76 and thrombosis following treatment with the integrin alphaIIbbeta3 antagonist eptifibatide are rare

77 r PP1, the Syk kinase inhibitor R406 and the integrin alphaIIbbeta3 antagonist lotrafiban.

78 nding site and a cytoplasmic LIBS epitope in integrin alphaIIbbeta3 are conformationally and function

79 Antagonists of the platelet integrin alphaIIbbeta3 are potent anti-thrombotic drugs,

80 brinogen binding, glycoprotein (GP)IIb-IIIa (integrin alphaIIbbeta3) becomes associated with the cyto

81 The platelet integrin alphaIIbbeta3 binds to a KQAGDV motif at the fi

82 V/W)CRAD(K/R)RC) and high specificity toward integrin alphaIIbbeta3 but not to other RGD binding inte

83 activated from resting conformations of the integrin alphaIIbbeta3 can be regulated by limited amino

84 The plasma-membrane integrin alphaIIbbeta3 (CD41/CD61, GPIIbIIIa) is a major

85 In CHO cells expressing integrin alphaIIbbeta3, co-expression of K2 with talin h

86 d that a subpopulation of the major platelet integrin, alphaIIbbeta3, co-sediments from detergent lys

87 Human or mouse platelets lacking functional integrin alphaIIbbeta3 complexes and human platelets pre

88 Integrin alphaIIbbeta3 contains an on/off switch that re

89 earrangements induced by these agents in the integrin alphaIIbbeta3 correlate with its ability to bin

90 r, deficiency of Dok-2 leads to dysregulated integrin alphaIIbbeta3-dependent cytosolic calcium flux

91 As expected, an antibody to the platelet integrin alphaIIbbeta3 did not inhibit endothelial cell-

92 rmined three-dimensional structures of human integrin alphaIIbbeta3 embedded in a lipid bilayer (nano

93 emonstrated that fibrinogen, an activator of integrin alphaIIbbeta3, enhances SERT activity in human

94 mong strongly stimulated adherent platelets, integrin alphaIIbbeta3 epitope changes, mPTP formation,

95 utively active platelet receptor GPIIb/IIIa (integrin alphaIIbbeta3) expressed on Chinese hamster ova

96 Expression of wild-type Rasa3 in integrin alphaIIbbeta3-expressing CHO cells blocked Rap1

97 mediate signaling via the platelet-specific integrin alphaIIbbeta3, fibrinogen binding was induced b

98 to select monoclonal antibodies specific to integrin alphaIIbbeta3 from a synthetic human antibody l

99 in a conformational change of the prototype integrin alphaIIbbeta3 from an inactive to an active sta

100 ombinant forms, to platelets and to purified integrins alphaIIbbeta3 (from platelets) and alphaVbeta3

101 nhibition of platelet activation or platelet integrin alphaIIbbeta3 function abolished M21 cell attac

102 In the absence of CypD, integrin alphaIIbbeta3 function was accentuated in both

103 of lipid kinases plays an important role in integrin alphaIIbbeta3 function, thereby supporting thro

104 ne domains are involved in the regulation of integrin alphaIIbbeta3 function.

105 ding onto fibrinogen resulting from enhanced integrin alphaIIbbeta3 function.

106 Integrin alphaIIbbeta3 functions as the fibrinogen recep

107 the cytosolic tails of the adhesion receptor integrin alphaIIbbeta3, fused to a coiled-coil construct

108 he human blood platelet fibrinogen receptor, integrin alphaIIbbeta3 (glycoprotein IIb-IIIa) is an arc

109 ic agents target the interaction of platelet integrin alphaIIbbeta3 (GPIIb-IIIa) with fibrinogen duri

110 Ligand binding to the platelet integrin alphaIIbbeta3 (GPIIb-IIIa), a prerequisite for

111 a Glanzmann's patient, who lacked functional integrin alphaIIbbeta3 (GPIIb-IIIa).

112 The interaction of fibrinogen with integrin alphaIIbbeta3 (GPIIb/IIIa), in part mediated by

113 Integrin alphaIIbbeta3 has served as an excellent model

114 Structural data of integrin alphaIIbbeta3 have been interpreted as supporti

115 Previous studies of integrin alphaIIbbeta3 have shown that both F2 and F3 bi

116 re equivalent in their ability to coactivate integrin alphaIIbbeta3 in a CHO cell system when coexpre

117 he platelet activation marker P-selectin and integrin alphaIIbbeta3 in its active conformation.

118 onstitutively associates with the prototypic integrin alphaIIbbeta3 in platelets and in cell lines ov

119 reading were diminished due to inhibition of integrin alphaIIbbeta3 in platelets from mice expressing

120 ression levels of collagen receptor GPVI and integrin alphaIIbbeta3 in platelets were not affected by

121 here that Src associates constitutively with integrin alphaIIbbeta3 in platelets.

122 platelet function is decreased activation of integrin alphaIIbbeta3 in response to stimulation with a

123 is study, we show that JAM-A associates with integrin alphaIIbbeta3 in resting platelets and dissocia

124 Integrin alphaIIbbeta3 inactivation was unchanged in pla

125 associated regulatory proteins, resulting in integrin alphaIIbbeta3 inactivation, and demonstrate a n

126 3 cytoplasmic domain, coincided closely with integrin alphaIIbbeta3 inactivation.

127 The clinically most widely used integrin alphaIIbbeta3 inhibitor abciximab is a chimeric

128 ir inhibition of platelet aggregation, three integrin alphaIIbbeta3 inhibitors are clinically approve

129 Ex vivo aggregation, secretion, and integrin alphaIIbbeta3 inside-out activation induced by

130 es SERT activity in human platelets and that integrin alphaIIbbeta3 interacts directly with the C ter

131 Integrin alphaIIbbeta3 is a highly abundant heterodimeri

132 messenger-mediated inside-out activation of integrin alphaIIbbeta3 is a key step in platelet aggrega

133 Integrin alphaIIbbeta3 is a member of the integrin famil

134 Integrin alphaIIbbeta3 is a necessary cofactor for PAR1

135 Aggregation of platelets via activated integrin alphaIIbbeta3 is a prerequisite for thrombus fo

136 The integrin alphaIIbbeta3 is a transmembrane (TM) heterodim

137 -in and inside-out approach at exploring how integrin alphaIIbbeta3 is activated.

138 The platelet integrin alphaIIbbeta3 is critical in hemostasis, and th

139 How integrin alphaIIbbeta3 is discouraged from spontaneous a

140 In contrast, outside-in signaling by integrin alphaIIbbeta3 is not altered, but it is inhibit

141 Fibrinogen binding by integrin alphaIIbbeta3 is promoted by platelet agonists

142 Function of the platelet integrin alphaIIbbeta3 is regulated by agonist-generated

143 Integrin alphaIIbbeta3 is widely known to regulate the p

144 xpression of the platelet-adhesion receptor, integrin alphaIIbbeta3, is caused by the presence of reg

145 ation, with particular focus on the platelet integrin alphaIIbbeta3, is provided in this review.

146 The major platelet integrin, alphaIIbbeta3, is required for platelet intera

147 ion of platelet thrombi is determined by the integrin alphaIIbbeta3-mediated interactions of platelet

148 n important role for Rasa3 in PI3K-dependent integrin alphaIIbbeta3-mediated outside-in signaling and

149 was previously identified as an inhibitor of integrin alphaIIbbeta3-mediated outside-in signaling and

150 of talin1 resulted in dramatically impaired integrin alphaIIbbeta3-mediated platelet aggregation and

151 h Ras and Rap1GAP activity and do not affect integrin alphaIIbbeta3-mediated spreading of CHO cells o

152 pressing CHO cells blocked Rap1 activity and integrin alphaIIbbeta3-mediated spreading on fibrinogen.

153 Fibrinogen binding to integrin alphaIIbbeta3 mediates platelet aggregation and

154 Fibrinogen binding to the integrin alphaIIbbeta3 mediates platelet aggregation and

155 The platelet integrin alphaIIbbeta3 mediates platelet aggregation and

156 The major membrane protein on platelets, integrin alphaIIbbeta3, mediates this response by rapidl

157 For the employed integrin alphaIIbbeta3 model system, the methodology ide

158 Thrombin, Ca(2+), the integrin alphaIIbbeta3, myosin IIa, FXIIIa cross-linking

159 body targeted against active conformation of integrin alphaIIbbeta3 on PMP surface.

160 nds upon the binding of adhesive proteins to integrin alphaIIbbeta3 on the platelet surface.

161 tte encoding human integrin beta3 to restore integrin alphaIIbbeta3 on the surface of megakaryocytes

162 rine (PS) exposure and epitope modulation of integrin alphaIIbbeta3 or a loss of binding of activatio

163 by which platelets regulate the function of integrin alphaIIbbeta3 (or GPIIb/IIIa), the platelet fib

164 ly characterized mouse line with a defect in integrin alphaIIbbeta3 outside-in signaling that affects

165 in in human platelets and a key regulator of integrin alphaIIbbeta3 outside-in signaling.

166 sing the possibility that it participates in integrin alphaIIbbeta3 outside-in signaling.

167 Integrin alphaIIbbeta3 plays a central role in hemostasi

168 Integrin alphaIIbbeta3 plays a critical role in platelet

169 Integrin alphaIIbbeta3 plays a pivotal role in hemostasi

170 According to current dogma, the integrin alphaIIbbeta3 plays an exclusive role in linkin

171 We present new evidence that the integrin, alphaIIbbeta3, plays a key role in trophoblast

172 where abnormalities of the platelet-specific integrin, alphaIIbbeta3, prevent platelet aggregation fo

173 ructural alteration modifies the function of integrin alphaIIbbeta3, priming the integrin for outside

174 k-2 is tyrosine-phosphorylated downstream of integrin alphaIIbbeta3, raising the possibility that it

175 We analyzed missense variants in the integrin alphaIIbbeta3 receptor subunit genes ITGA2B and

176 Bidirectional signaling of integrin alphaIIbbeta3 requires the beta3 cytoplasmic do

177 ction of the fibrinogen receptor GPIIb/IIIa (integrin alphaIIbbeta3), respectively.

178 Platelet integrin alphaIIbbeta3 responds to intracellular signals

179 K) from the CBD of TS1 activate the platelet integrin alphaIIbbeta3, resulting in platelet spreading

180 which the low-affinity state of the platelet integrin alphaIIbbeta3 results from alphaIIbbeta3 adopti

181 Inactivation of integrin alphaIIbbeta3 reverses platelet aggregate forma

182 nstrates that two orthogonal events regulate integrin alphaIIbbeta3's interactions with fibrinogen, i

183 Outside-in integrin alphaIIbbeta3 signaling is required for normal

184 aling effector downstream of both G12/13 and integrin alphaIIbbeta3 signaling, which contributes to t

185 Binding of platelets to fibrinogen via integrin alphaIIbbeta3 stimulates cytoskeletal reorganiz

186 hin the extracellular domain of the platelet integrin alphaIIbbeta3 that exhibits many properties tha

187 st beta3 (GPIIIa49-66), a region of platelet integrin alphaIIbbeta3 that is unique.

188 ide LSARLAF, that specifically activates the integrin alphaIIbbeta3 (the fibrinogen receptor), the PD

189 of platelet surface receptors, including the integrin alphaIIbbeta3, the immunoreceptor tyrosine-base

190 s of interactions between fibrinogen and the integrin alphaIIbbeta3, the ligand-receptor pair essenti

191 Integrin alphaIIbbeta3, the most abundant integrin in pl

192 Thus, in the case of one integrin, alphaIIbbeta3, the molecular requirements for

193 latelet binding was promoted by the platelet integrin alphaIIbbeta3: the bacterium bound to purified

194 ent to activate and extend membrane-embedded integrin alphaIIbbeta3, thereby resolving numerous contr

195 g platelets into the thrombus, and activates integrin alphaIIbbeta3 through a pathway that is depende

196 l GPIa/IIa (integrin alpha2beta1) instead of integrin alphaIIbbeta3, thus explaining the clinically m

197 ermal titration calorimetry (ITC) to measure integrin alphaIIbbeta3 TM complex affinity, to study the

198 The structurally unique, highly conserved integrin alphaIIbbeta3 TM complex rationalizes bi-direct

199 l and thermodynamic basis of proline-induced integrin alphaIIbbeta3 TM complex stabilization to under

200 Here, we report the structure of the integrin alphaIIbbeta3 TM complex, structure-based site-

201 he homomeric and heteromeric interactions of integrin alphaIIbbeta3 TM helices in biological membrane

202 The regulated ability of integrin alphaIIbbeta3 to bind fibrinogen plays a crucia

203 The regulated ability of integrin alphaIIbbeta3 to bind fibrinogen plays a crucia

204 smembrane helix of the beta3 subunit enabled integrin alphaIIbbeta3 to constitutively bind soluble fi

205 e platelet surface receptors FcgammaRIIA and integrin alphaIIbbeta3 to induce platelet activation, wh

206 Both functions allow the integrin alphaIIbbeta3 to mediate platelet aggregation.

207 phosphatases are critical for the ability of integrin alphaIIbbeta3 to support stable platelet adhesi

208 n erythroleukemia (HEL) cells, which express integrin alphaIIbbeta3, to investigate whether phosphory

209 The integrin alphaIIbbeta3 transmembrane (TM) complex is sta

210 Integrin alphaIIbbeta3 undergoes a conformational change

211 dding, exposure of P-selectin, and activated integrin alphaIIbbeta3 upon activation with thrombin wer

212 n the selectivity of these Fab molecules for integrin alphaIIbbeta3 versus the vitronectin receptor a

213 ate and inactivate RhoA, and the function of integrin alphaIIbbeta3 was studied.

214 ansmembrane domains regulate the activity of integrin alphaIIbbeta3, we synthesized a soluble peptide

215 ation of Rap1b and inside-out stimulation of integrin alphaIIbbeta3 were reduced after inhibition of

216 signals that drive their activation through integrin alphaIIbbeta3,which serves to prevent inappropr

217 iates the rapid but reversible activation of integrin alphaIIbbeta3, while the adenosine diphosphate

218 rsubunit disulfide bond, we generated mutant integrin alphaIIbbeta3 with blocked transmembrane separa

219 a4 tail to alphaIIb resulted in a complex of integrin alphaIIbbeta3 with paxillin.