ライフサイエンスコーパス: interchain

1 The interchain (13)C-(19)F dipolar coupling measured in a ro

2 We present a method to probe intra- and interchain activities within dimeric nonribosomal peptid

3 2 domain makes a minimal contribution to the interchain affinity in factor VIII.

4 tibody, indicating that equilibration of the interchain and intrachain hinge disulfide pairing was no

5 zation stems from the appropriate balance of interchain and intrachain metal ion coordination by Gla

6 ists of two polypeptide chains linked by two interchain and one intrachain disulfide bond.

7 or interfaces formed between polymer chains (interchain) and within a polymer chain (intrachain).

8 part of the same trend, due to the different interchain arrangement associated with the phase change.

9 s very similar to one of the natural dimeric interchain associations evident in the structure of solu

10 The emissions are caused by intrachain and interchain aurophilic interactions between the linear Au

11 in hydrogen bond, which confirms that direct interchain backbone hydrogen bonding is a major force fo

12 articipation in disulfide linkage, which was interchain based on the direct correlation between preva

13 Interchain beta-sheet (ICBS) interactions occur widely i

14 guide the development of agents that control interchain beta-sheet interactions and that the database

15 624 cm(-1) was characteristic of the nascent interchain beta-sheets, consistent with protein aggregat

16 domains contribute approximately 90% of the interchain binding energy in factor VIII and that A3 dom

17 contain mutations which affect one probable interchain bond were nonviable and could not be propagat

18 bonds and is connected to the virion by one interchain bond.

19 ositol anchor, which dimerizes through three interchain bridges.

20 Our results suggest that interchain Ca(2+) coordination in con-T[K7gamma] is incu

21 teractions include numerous salt bridges and interchain cation-pi interactions, but not intramolecula

22 rly two orders of magnitude greater than the interchain charge transport along the pi-pi stacking dir

23 s is the first example to clarify intra- and interchain charge transport based on an individual singl

24 ggered triple helical species facilitated by interchain charged pairs, and is inspired by similar sti

25 elopeptide sites, and between the individual interchain combinations.

26 her strengthens or weakens one of the native interchain contacts (prevalent in the transition state e

27 reasing the counteranion (CA) size decreases interchain contacts and aggregation and leads to a subst

28 yp-Gly)5 shows evidence for the existence of interchain contacts between alpha-carbon hydrogens from

29 eutral pH, we observed weak N- to C-terminal interchain contacts driven by electrostatic interactions

30 Increased interchain contacts give rise to a lowering of the photo

31 Mixed-charge clusters are prominent at interchain contacts where they stabilize quaternary prot

32 and wrap around one another, with extensive interchain contacts.

33 g transition state characterized by very few interchain contacts.

34 n be either increased or decreased by tuning interchain cooperativeness via the introduction of a sin

35 ted structures to show that the reduction in interchain coupling is not due simply to increased packi

36 o-dimensional lamellar structures, increased interchain coupling strongly influences the traditional

37 little effect of electrostatic disorder and interchain coupling.

38 few chains, prohibiting long-range effective interchain coupling.

39 lky iodo-substituent packs within a nonpolar interchain crevice.

40 The potential for interchain cross-link formation by the analogous crotona

41 esented for the formation of a carbinolamine interchain cross-link in 5'-CpG-3' sequences, arising fr

42 used to explain the 5'-d(CpG) preference for interchain cross-linking by acrolein.

43 ble, polymeric nanogels using a simple intra/interchain cross-linking reaction.

44 tail domain residues were used for extensive interchain cross-linking.

45 ine on the tail domain were used for limited interchain cross-linking.

46 Six interchain cross-links distinguished between alternative

47 that S,S-DEB produced the highest number of interchain cross-links in 5'-GGC-3'/3'-CCG-5' sequences.

48 part, why the (6S,8R,11S) stereoisomer forms interchain cross-links in the 5'-CpG-3' sequence whereas

49 Interchain cross-links represent one of the most serious

50 oxynucleotide duplexes containing 2a to form interchain cross-links was evaluated by HPLC, CZE, MALDI

51 Here, we present a comparative study of interchain cysteine linked IgG1 ADCs and the correspondi

52 ges were due to the presence of drugs on the interchain cysteine residues or the absence of intact in

53 resence of vcMMAE or mcMMAF on the alkylated interchain cysteine residues.

54 t of the intact mass of an ADC conjugated at interchain cysteine residues.

55 lt from the attachment of drug conjugates to interchain cysteine residues.

56 trulline-monomethyl Auristatin E (vcMMAE) at interchain cysteine residues.

57 Cs, mAbs, reduced mAbs (containing 8 reduced interchain cysteine thiols), and partially reduced mAbs

58 d previously in the literature for analyzing interchain cysteinyl-linked ADCs are either not amenable

59 the reference method for quality control of interchain cysteinyl-linked ADCs.

60 is33 or His26 and have been used to estimate interchain diffusion rates of the protein.

61 constant should not be used as a measure of interchain diffusion, and the results emphasize the impo

62 hains that eliminate the restrictions on the interchain displacements, leading to the opening of the

63 The variation is attributed to increased interchain distance corresponding to decreased interchai

64 e of two separate peaks corresponding to the interchain distances within the crystalline lattices of

65 c-TeNT(RY) at C439S and C467S eliminated the interchain disulfide bond and inhibited betalac transloc

66 ted by electron transfer and addition to the interchain disulfide bond between Cys(215) of the light

67 l fragment ions due to the protection of the interchain disulfide bond between light and heavy chain,

68 carboxy termini and stabilized by forming an interchain disulfide bond between the Cys39 residues of

69 ed an improved scFab format that retains the interchain disulfide bond by increasing the linker lengt

70 T and TeNT are dichain proteins linked by an interchain disulfide bond comprised of an N-terminal cat

71 either engineered surface-exposed or reduced interchain disulfide bond cysteine residues.

72 ch the VH-VL heterodimer is stabilized by an interchain disulfide bond engineered between structurall

73 in and prepro-von Willebrand factor promotes interchain disulfide bond formation among polypeptides i

74 Interchain disulfide bond formation indicates that both

75 ro-von Willebrand factor is also involved in interchain disulfide bond formation, which is consistent

76 Under nonreducing conditions where the interchain disulfide bond is intact, the enzymatically i

77 We conclude that: 1) the Cys6 interchain disulfide bond of SP-A is required for aggreg

78 e buffer containing zinc and DTT reduced the interchain disulfide bond releasing and activating the c

79 However, this format required removal of the interchain disulfide bond to achieve modest display leve

80 of C8 alpha and C8 gamma in which the single interchain disulfide bond was eliminated.

81 Under an acidic condition, the interchain disulfide bond was selectively cleaved with t

82 e light chain cysteine residue that forms an interchain disulfide bond with the cysteine residue in t

83 betalac-TeNT(RY) retained the interchain disulfide bond, showed ganglioside-dependent

84 ic specificity of the TCR do not require its interchain disulfide bond, transmembrane segments, or gl

85 each chain was common to peptides having an interchain disulfide bond, while for peptides having int

86 in to promote the formation of an additional interchain disulfide bond.

87 ting the presence of at least one additional interchain disulfide bond.

88 minal of both sequences, which could mediate interchain disulfide bonding, and 11 of the 14 aromatic

89 ere are cysteines in conserved positions for interchain disulfide bonding, and there is a conserved t

90 suggesting that the N termini are linked by interchain disulfide bonds and are presented on the cell

91 in the ADCs was due to the absence of intact interchain disulfide bonds and not the presence of vcMMA

92 Isoform A contains two covalent interchain disulfide bonds at heavy chain positions 239

93 The two endogenous interchain disulfide bonds between Cys16 and Cys37 in RI

94 nd 242, while isoform B fails to develop any interchain disulfide bonds but has 239-242 intrachain di

95 ies, which can be achieved by first reducing interchain disulfide bonds followed by conjugation of th

96 y performing online EC-assisted reduction of interchain disulfide bonds in an intact mAb, the release

97 44, and Cys13246) may be involved in forming interchain disulfide bonds in mucin dimers.

98 ked oligosaccharide, the collagen helix, and interchain disulfide bonds in SP-D assembly and secretio

99 sociation of these subunits is stabilized by interchain disulfide bonds involving two conserved amino

100 Head-to-tail interchain disulfide bonds link subunits within the homo

101 Furthermore, it was found that reduction of interchain disulfide bonds occurs in the ESI source depe

102 ted trisulfide bonds are associated with the interchain disulfide bonds of both A isoform and A/B iso

103 at the alpha chains are covalently linked by interchain disulfide bonds only between the two cysteine

104 /D is a compact docking module, with unusual interchain disulfide bonds that help maintain the AKAP i

105 in also influenced the susceptibility of the interchain disulfide bonds to attack by reducing agents

106 ysteine at position 23, which forms aberrant interchain disulfide bonds, causes disruption of the nor

107 with cysteine residues that were involved in interchain disulfide bonds.

108 ysteine thiols generated by the reduction of interchain disulfide bonds.

109 of the cysteines required for intrachain and interchain disulfide bonds.

110 equence or loss (by mutation) of the insulin interchain disulfide bonds.

111 o antiparallel B chains held together by two interchain disulfide bonds.

112 te the presence of at least two NH2-terminal interchain disulfide bonds.

113 ules are physically linked to each other via interchain disulfide bonds.

114 rved cysteine residues, which participate in interchain disulfide bonds.

115 eta can form malfolded structures containing interchain disulfide bonds; malfolding is correlated wit

116 Both cysteine 524 and cysteine 682 form interchain disulfide bonds; their conversion to serine r

117 ise serine substitution of insulin's exposed interchain disulfide bridge (Cys(A7)-Cys(B7)) are charac

118 Additionally, interchain disulfide bridge experiments showed that the

119 y through an Ile/Leu hydrophobic core and an interchain disulfide bridge.

120 involves low pH, proteolysis, and an intact interchain disulfide bridge.

121 Secreted proenzyme formed non-native, interchain disulfide bridges and displayed only residual

122 ix formation in the collagen-like domain and interchain disulfide bridges at the NH2 terminus.

123 from Cys --> Ser replacements was found for interchain disulfide bridges involving the four cysteine

124 Nature uses interchain disulfide bridges to stabilize collagen trime

125 of an adjacent collagen triple helix to form interchain disulfide bridges.

126 re structure where they form both intra- and interchain disulfide bridges.

127 The Cys-13 should be available for interchain disulfide bridging and consequent aggregation

128 of chicken gizzard tropomyosin with a single interchain disulfide cross-link.

129 A class of ADCs that utilize the reduced interchain disulfide cysteine residues for drug attachme

130 numbers of drugs linked to different former interchain disulfide cysteine residues on the antibodies

131 se data support the requirement of an intact interchain disulfide for LC translocation and imply that

132 Disruption of interchain disulfide formation at Cys(-)(1) by substitut

133 at only Cys6 in this region is available for interchain disulfide formation.

134 spholipids into type II cells; 3) N-terminal interchain disulfide linkage can functionally replace th

135 two closely related isoforms, that requires interchain disulfide linkage for several functions inclu

136 These processes also occur when interchain disulfide linkage is inhibited, indicating th

137 l for function, the importance of the second interchain disulfide linkage within the N-terminal Isole

138 does not require the collagen-like region or interchain disulfide linkage; 2) the N-terminal portion

139 to some recombinant proteins with intra- or interchain disulfide linkages are presented.

140 e-stranded coiled coils, and surface-exposed interchain disulfide linkages mediate the formation of t

141 zation of both "left-arm" and "right-arm" HL interchain disulfide peptides and observed that native H

142 Dynamic interchain disulfide rearrangement, with slow kinetics,

143 A dimeric species, stabilized by an interchain disulfide, appears to be involved in the asse

144 The recombinant NC1 formed interchain disulfide-bonded dimers and trimers and was N

145 In contrast to the numerous examples of interchain disulfide-linked aggregates, factor VIII is t

146 isomerization was resolved by engineering an interchain disulfide.

147 The interchain disulfides between the light and heavy chains

148 light and heavy chains were weaker than the interchain disulfides between the two heavy chains.

149 n cysteine residues or the absence of intact interchain disulfides or both.

150 ins IgG and IgE, but has differently located interchain disulphide bonds and external rather than int

151 The small interchain electron effective mass is comparable to the

152 In contrast to rr-P3HT, interchain energy migration in poly(3-(2'-methoxy-5'-oct

153 hexylthiophene) (rr-P3HT) enables long-range interchain energy migration, while disordered packing in

154 The different intrachain and interchain energy transfer time scales explain the behav

155 at polymer/copolymer concentrations near the interchain entanglement threshold.

156 -average molar mass (M(w)) and the extent of interchain entanglements (c/c).

157 emonstrates that the N17 module also reduces interchain entanglements between polyQ domains.

158 Therefore, the combination of a reduction of interchain entanglements through homopolymeric polyQ and

159 ely matched for molar mass and the extent of interchain entanglements.

160 1) K, where J(1) and J(2) are the intra- and interchain exchange couplings, respectively, which consi

161 ordering at T(N) = 7 K due to intrachain and interchain exchange interactions.

162 Interchain exchange is estimated to be 33- to 150-fold w

163 The interchain exchange is estimated to be zJ/k congruent wi

164 terchain distance corresponding to decreased interchain exchange, when more F4BImNN is added into the

165 arser phase segregation and formation of the interchain excited states that are energetically unfavor

166 s the slow phase is attributed to intra- and interchain exciton diffusion to the HMIDC.

167 ingle-chain aggregates, implying rather weak interchain excitonic coupling and energy transfer.

168 omain that lead to preferential formation of interchain excitons delocalizing over more than one poly

169 Such energetics of the interchain excitons in low-bandgap copolymers calls for

170 We propose that such interchain H-bonding may destabilize metal binding in th

171 Stable intrachain and interchain H-bonds are identified as a function of tempe

172 mers of a single cellulose polymer chain and interchain H-bonds between adjacent chains.

173 f-association to form a coiled-coil or other interchain helical structure.

174 thermal broadening becomes comparable to the interchain hopping energy remains an unresolved issue, o

175 ypothesize that the methyl group shields the interchain hydrogen bond between the glycine and the Xaa

176 luded in MP2, is a significant factor in the interchain hydrogen bond energies.

177 isostere peptide that retained the backbone interchain hydrogen bond, which confirms that direct int

178 in the X position suggests the importance of interchain hydrogen bonding directly or through water to

179 small but cooperative decrease on the total interchain hydrogen bonding energy.

180 Our significant finding is that interchain hydrogen bonding is greatly affected by varia

181 eneral feature of triple-helical structures: interchain hydrogen bonds are always longer and weaker t

182 Interchain hydrogen bonds contribute to the interaction

183 ond instance of water mediated N--H ... O==C interchain hydrogen bonds for the amide group of the res

184 rences in solvent shielding of the essential interchain hydrogen bonds may result in differences in s

185 with a buried surface area of 1738 A2 and 33 interchain hydrogen bonds resulting from C-terminal stra

186 line, and that residue side chains also form interchain hydrogen bonds with frequencies that are depe

187 nge rate for glycine NH residues involved in interchain hydrogen bonds.

188 residue, a high content of imino acids, and interchain hydrogen bonds.

189 s, and this ratio compensates for the weaker interchain hydrogen bonds.

190 ributions of the beta-ladders in the overall interchain interaction and compute first- and second-ord

191 imple model that also yields a value for the interchain interaction energy.

192 The interchain interaction is unstable with respect to the f

193 is varied to tune the molecular packing and interchain interaction of the polymers in order to eluci

194 these types of residues are those that make interchain interaction only through the protein main cha

195 In addition, three distinct DNA interchain interaction regimes were found to exist, due

196 tematic study of the roles of crystallinity, interchain interaction, and exciton delocalization on ul

197 nticipated key role of GPI-AP in FcepsilonRI interchain interactions and early FcepsilonRI signaling

198 orphology-dependent variations of intra- and interchain interactions and order in poly-3-hexylthiophe

199 onequivalence of these positions in terms of interchain interactions and solvent exposure.

200 ation greatly influences both intrachain and interchain interactions and ultimately the degree of pha

201 tions, while at low pH, the C- to C-terminal interchain interactions are significantly stronger and d

202 ystem) between protein fragments propitiates interchain interactions at early stages of the folding p

203 species originate from excimers produced by interchain interactions being mediated by the particular

204 The six interchain interactions between Glu-239 in one catalytic

205 es in collagen are characterized in terms of interchain interactions between non-imino acid X and Y r

206 Interchain interactions between pi-systems have a strong

207 These transient interchain interactions coupled with a non-A beta amyloi

208 we systematically investigated non-covalent interchain interactions for CH3 domains in the other hum

209 e study the impact that the resulting strong interchain interactions have on the photophysical proper

210 gregation providing insight into the role of interchain interactions in these subsecond switching ele

211 he hybrid enzyme only three of the usual six interchain interactions involving Glu-239 are sufficient

212 This suggests that interchain interactions of pi-systems over their entire

213 netic ground state, stemming from weak pi-pi interchain interactions of strength J( perpendicular)/k(

214 The impact of interchain interactions on the charge carrier effective

215 The effects of interchain interactions on the component and state separ

216 to an increase in the strength and number of interchain interactions that are induced by organic comp

217 Characterization of these first interchain interactions will provide fundamental insight

218 Observation of a network of interchain interactions, as established by NOE spectrosc

219 e N-terminal region may arise from transient interchain interactions, suggesting that the N-terminal

220 The BBT moiety also strengthens interchain interactions, which provides higher thermal s

221 bamoylase is governed by specific intra- and interchain interactions.

222 he packing geometry of the molecules and the interchain interactions.

223 h 85 long-range NOE cross peaks arising from interchain interactions.

224 485, and 508 and glutamines 465 and 489 for interchain involucrin cross-links.

225 r Lys but not for Arg and can be assigned to interchain ion pairs, as shown by molecular modeling.

226 placing one arginine residue, which forms an interchain ionic interaction with a glutamic acid residu

227 ecific distribution of hydrophobic residues, interchain ionic interactions can be crucial in modulati

228 Trisulfides were detected only in interchain linkages and were predominantly in the light-

229 weight polymers with triazole- and urea-type interchain links, respectively.

230 st a few chains, can dramatically impede the interchain mechanism.

231 rphology on energy migration in CP, tailored interchain morphologies were achieved using solvent vapo

232 ormational variability results in disordered interchain morphology even between a few chains, prohibi

233 To probe the effect of interchain morphology on energy migration in CP, tailore

234 he actual displacements were decomposed into interchain motions and intrachain deformations.

235 he transmembrane helices mainly results from interchain motions that already take place in the closed

236 re presumably much weaker in energy than the interchain N-H...O=C hydrogen bonds responsible for the

237 tal number of native contacts, the number of interchain native contacts, and the total conformational

238 The strength of these interchain NH(Val)...O=C hydrogen bonds varies in the or

239 lting transition, and observation of several interchain NOEs.

240 es that are consistent with the formation of interchain, nonnative disulfide bridges and the establis

241 mination program CYANA to build a network of interchain nuclear Overhauser effect constraints that ca

242 nks loricrin and SPRs together to form small interchain oligomers, which are then permanently affixed

243 ulated phenalenyls along [0 0 1], and (2) an interchain overlap involving a pair of carbon atoms (C4)

244 -chain conformation and hence highly ordered interchain packing in aggregates.

245 Surprisingly, the ordered and close interchain packing in F-P3EHT does not lead to strong ex

246 We report that highly ordered interchain packing in regioregular poly(3-hexylthiophene

247 ymes that incorporate l-amino acids into the interchain peptide bridge of Gram-positive cell wall pep

248 X enzyme that initiates the synthesis of the interchain peptide of the peptidoglycan in a subset of b

249 rge family of genes previously implicated in interchain peptide synthesis but with unknown specific f

250 First, P3HT-SH nanofibers were formed due to interchain pi-pi stacking.

251 For a mixing time of 1200 ms, 5 A interchain proximities appear.

252 e elucidated the pre-steady-state intra- and interchain rates and the corresponding flux of the acyla

253 it is likely that they contain intra- and/or interchain repulsions by acidic residues.

254 tein interaction interfaces make exclusively interchain residue-type-independent contacts.

255 Site-directed mutagenesis identified an interchain salt bridge (Lys(48)-Glu(47')) in the RHH dom

256 ine, a change that results in the loss of an interchain salt bridge between alphaArg76 and betaAsp57

257 employed to ascertain the role of intra- and interchain salt-bridges in the folding and stability of

258 nduced charge separation, in contrast to the interchain separation achieved in conventional donor-acc

259 hain conductors with different values of the interchain single-electron transfer integral tb, which q

260 keratin filament terminals and increases the interchain spacing of the filaments.

261 xcited state structures, and dynamics of the interchain species by combined ultrafast spectroscopy an

262 ion pathways originating from intrachain and interchain species.

263 e similar to those of wild-type insulin, the interchain tether constrains the extent of conformationa

264 We find that despite the interchain transport becoming non-metallic, the charge c