ライフサイエンスコーパス: interhelical

1 Interhelical (15)N...(13)C REDOR measurements between Gl

2 The interhelical (19)F-(19)F distances show a bimodal distri

3 er system to examine the contribution of the interhelical a-a' interaction to dimer stability for six

4 vely reproduce previously reported trends in interhelical a-a' side-chain pairing preferences at the

5 R64V in the putative calcium-binding lumenal interhelical a-b loop of the photosystem II (PSII) D1 pr

6 D1-D61E in the putative Ca2+-binding lumenal interhelical a-b loop of the photosystem II (PSII) D1 pr

7 Analysis of the statistics of the interhelical and intrahelical (13)C-(13)C distances in t

8 Evaluation of predicted interhelical and lipid-exposed faces of this TM segment

9 els showing the stabilized conformations and interhelical and receptor-ligand contacts corresponding

10 and binding energy and a significant gain in interhelical and receptor-ligand hydrogen bonds.

11 Apart from a slight widening of the interhelical angle between alpha-helices A and B and cha

12 We examine the interhelical angle distribution as a function of spatial

13 ades ago to explain perceived preferences in interhelical angle distributions.

14 tween specific position of methyl groups and interhelical angle is found for parallel and antiparalle

15 that depend on geometric parameters such as interhelical angle must occur before the data is binned

16 ix 3 (and 3') of S100A1 was found to have an interhelical angle of -150 degrees with helix 4 (and 4')

17 but more similar to apo-S100B, which has an interhelical angle of -166 degrees.

18 The addition of calcium did not change the interhelical angle of helices 1 and 2 in the pseudo EF-h

19 2+ binding does not significantly change the interhelical angle of helixes 1 and 2 in the pseudo EF-h

20 However, the interhelical angle of helixes 3 and 4 in the normal EF-h

21 rs has two distinct helical domains, with an interhelical angle within 60-100 degrees, ruling out the

22 the size of amino acid and magnitude of the interhelical angle.

23 IL-8, MGSA, or NAP-2 results from differing interhelical angles and separations.

24 Interhelical angles are found to correlate with the conf

25 ogram arrays have been used to determine the interhelical angles for a variety of DNA branched juncti

26 Applied for the prediction of interhelical angles in glutathione S-transferase, intrac

27 elix-helix interfaces with similar values of interhelical angles not only in homologous proteins but

28 ion is planar with approximately 120 degrees interhelical angles, whereas S15 and Mg2+ yield a juncti

29 erfaces influence arrangement of helices and interhelical angles.

30 ous and nonhomologous proteins and influence interhelical angles.

31 havior in MscS appears to depend on specific interhelical associations and the flexibility of the por

32 hat octasaccharide binds orthogonally to the interhelical axis and spans the dimer interface and that

33 They are dominated by long continuous interhelical base stacking, tend to segregate into domai

34 (-gamma(h)) and 3' (alpha(h)) helices and an interhelical bend angle (beta(h)).

35 the RNA adopts a 43 degrees (+/-4 degrees ) interhelical bend angle (beta) and displays large amplit

36 s that bind Zn(II) to a pair of tetrahedral, interhelical binding sites, with two ligands derived fro

37 inor groove and the presence of ion-mediated interhelical bonds and extensive hydration across the ma

38 The structure is stabilized by multiple interhelical C(alpha)H to C=O hydrogen bonds, characteri

39 The dimerization appears to be driven by interhelical Ca(2+) coordination between the following r

40 eucine and valine in the d position produced interhelical clashes between the Cgamma2 methyl groups w

41 y and does not seem to penetrate the TM2-TM3 interhelical clefts in MscS.

42 pathway A), and a few other pathways through interhelical clefts were also observed with significantl

43 a reversible switch that enables sampling of interhelical conformations that would otherwise be topol

44 We have sought specific interhelical constraint contacts, using the M(1) muscari

45 Additional interhelical constraints between Ile76 and Gly79 and bet

46 membrane (7-TM) receptors requires switching interhelical constraints that stabilize the inactive sta

47 t the most polar residues strongly influence interhelical contact formation, although they occur rare

48 This type of interhelical contact has received relatively little atte

49 bed knobs-into-holes packing interactions at interhelical contact surfaces are optimized so that side

50 alphatalpha motif is near the turn where the interhelical contacts are rather loose, while the motif'

51 ake place through the disruption of the weak interhelical contacts between helices 1 and 5.

52 +2) of the helix surface are central for the interhelical contacts in a four-alpha-helix bundle.

53 ment of solid-state NMR methods for studying interhelical contacts in membrane proteins, as well as f

54 ed approach is shown to successfully predict interhelical contacts in several membrane protein system

55 ving the breaking and reformation of E and F interhelical contacts in the R-T direction but not the T

56 determined the local secondary structure and interhelical contacts in the region of position 664 in p

57 l correlated with a high percentage of short interhelical contacts in the soluble protein.

58 st that hydrophobic burial along native-like interhelical contacts is important for the formation of

59 e proteins exhibit a broader distribution of interhelical contacts than helices in soluble proteins.

60 an important part of a network of conserved interhelical contacts that defines the off-state of a ge

61 of the procedure is a method for predicting interhelical contacts that is based on a helix-packing m

62 swivel repacks hydrophobic and electrostatic interhelical contacts within intracellular lipids, resul

63 cate hydrophobic side chains not involved in interhelical contacts, and (iii) extend hydrophobic side

64 Additional factors, including the interhelical contacts, the length, structure and flexibi

65 implicated in trimer formation forms strong interhelical contacts, Thr50 points to the inside of the

66 ins that buttress the core through predicted interhelical contacts, while smaller introns use loop-he

67 eral types of direct intrahelical as well as interhelical contacts.

68 topology, secondary structure, and numerous interhelical contacts.

69 obic side chains at residues not involved in interhelical contacts.

70 main mostly intact, but they completely lose interhelical contacts.

71 demonstrate that this potentially attractive interhelical Coulombic interaction has little or no infl

72 eterodimer, Acid-Ke-Base-Eg, suggesting that interhelical Coulombic interactions and a buried polar i

73 Interhelical Coulombic interactions between residues at

74 each peptide such that exclusively favorable interhelical Coulombic interactions can occur only in th

75 occur only in the antiparallel orientation, interhelical Coulombic interactions favor the parallel o

76 Here, the isotropic chemical shift data and interhelical cross peaks from magic angle spinning solid

77 a common anesthetic binding pocket within an interhelical dimerization interface.

78 GASright, which is characterized by a short interhelical distance and a right-handed crossing angle

79 determine the net attraction and equilibrium interhelical distance as a function of the chemistry, po

80 ovement of helices II and VII that increases interhelical distance by 3 to 4 A at the periplasmic end

81 ron resonance (DEER) is used here to measure interhelical distance changes induced by sugar binding t

82 An interhelical distance has been precisely measured by RED

83 Long-range interhelical distance measurements using magic angle spi

84 al orientations, by pseudodihedral angle and interhelical distance measures.

85 A dynamic variation in interhelical distance on the microsecond timescale is co

86 The short interhelical distance places the beta-hydroxyl of Thr-87

87 s, whereas magic-angle spinning data yielded interhelical distance restraints.

88 The interhelical distances (up to 12 A) reveal weak interact

89 gold nanoparticle assemblies, in particular interhelical distances and particle size.

90 Four intramolecular interhelical distances between helix pairs 1-4, 2-4, 3-4

91 e to obtain information about intermolecular interhelical distances between the helix 4 of one apolip

92 Changes observed here in interhelical distances in the N-terminus can be accounte

93 ing on the helices pairs, the intramolecular interhelical distances increased between 15 and > or = 2

94 sing angles of -58 degrees +/- 9 degrees and interhelical distances of 7.7 +/- 0.5 angstroms.

95 ucted using the helix tilt angle and several interhelical distances previously measured on unoriented

96 estimated intermolecular and intramolecular interhelical distances suggest a model in which the apol

97 Interhelical distances were measured using mixed (15)N/(

98 e peptides are significantly larger than the interhelical distances with comparable arginine peptides

99 Stability correlates with shorter interhelical distances, narrower crossing angles, better

100 nded upon membrane binding, thus lengthening interhelical distances.

101 to lipid is accompanied by a major change in interhelical distances.

102 ts of myoglobin designed to monitor selected interhelical distances.

103 ed by introducing single, double, and triple interhelical disulfide bonds to restrict the opening of

104 Introduction of an interhelical disulfide linkage in the ACP domain suppres

105 eviously that residues 85-120 of the SNAP-25 interhelical domain, which do not interact with syntaxin

106 b that affect the bend angle, direction, and interhelical dynamics are correlated with telomerase act

107 The cytoplasmic interhelical E-F loop in rhodopsin is a part of the regi

108 ssment of PB in prior studies, ensuring that interhelical electrostatic forces dominate the behavior

109 The repulsive interhelical electrostatic interactions that are used to

110 s are relatively unstable due to unfavorable interhelical electrostatic interactions within the Fos t

111 hydrophobic interface and (iii) presence of interhelical electrostatic interactions.

112 The three interhelical Euler angles define clockwise rotations aro

113 e we describe a protocol for computing three interhelical Euler angles describing the relative orient

114 Moreover, the nearest-neighbor interhelical F-F distance between (4-19F)Phe30 is 7.9-9.

115 ith alanines in positions 315, 319, and 323 (interhelical face) or 317, 321, and 325 (external lipid-

116 sidual dipolar coupling experiments revealed interhelical flexibility of P4.

117 (1) the presence of attractive or repulsive interhelical g<-->e' electrostatic interactions and (2)

118 ding, Ser273 phosphorylation also creates an interhelical g<-->e' salt bridge with Lys268 that increa

119 The two structures have similar interhelical geometries and are planar with hydrophobic

120 lix/turn/C-helix structure at pH 5 with open interhelical geometry and N-helix/turn/C-coil structure

121 In this study, the interhelical geometry of membrane-associated HAfp is pro

122 N-helix/turn/C-helix at both pHs with closed interhelical geometry.

123 convex face exposes a series of hydrophobic interhelical grooves.

124 thening of the Acdots, three dots, centeredE interhelical H bond, and weakening of the "switch" quate

125 Four interhelical H(alpha) to C horizontal lineO aliphatic H-

126 C=O hydrogen bonds, characterized by strong interhelical H(N)-H(alpha) and H(alpha)-H(alpha) NOE con

127 They show also that a full complement of interhelical H-bonds actually slows the initial quaterna

128 and other structural constraints, including interhelical H-bonds and two disulfide bridges (Cys(40)-

129 The buried interhelical H-bonds are found to be mainly located betw

130 Interhelical H-bonds existing in the mu-receptor were ap

131 Two sets of interhelical H-bonds involved residues conserved among A

132 d buried polar/ionizable residues and buried interhelical H-bonds located in the otherwise hydrophobi

133 peared to be mediated by a Tyr(307)-Glu(312) interhelical hydrogen bond and a Glu(319)-Arg(314) elect

134 model of a flexible periplasmic loop and an interhelical hydrogen bond between Glu26 and Tyr153.

135 the barrier crossing involves breaking of an interhelical hydrogen bond between helix5 and helix6, an

136 l is transmitted to HtrII from the energized interhelical hydrogen bond between Thr204 and Tyr174, wh

137 es contains triplets that may be involved in interhelical hydrogen bond interactions, suggesting the

138 lix interactions through the formation of an interhelical hydrogen bond, in other cases the strongly

139 this position prevents the formation of this interhelical hydrogen bond.

140 a critical role through the formation of an interhelical hydrogen bond.

141 e movements of helices 3 and 6 and transient interhelical hydrogen bonding between Ser-165 on transme

142 Closely packed helices, in turn, facilitate interhelical hydrogen bonding of both weakly polar (Ser,

143 rive transmembrane helix association through interhelical hydrogen bonding.

144 ceptor structure has an extensive network of interhelical hydrogen bonds and a ligand-binding crevice

145 two novel hydrophobic "proline Ncaps", four interhelical hydrogen bonds and short N- and C-terminal

146 ation by US11 thus requires the formation of interhelical hydrogen bonds within the ER membrane.

147 Water molecules mediate all but one of the interhelical hydrogen bonds, and many of the lattice int

148 erived from the ensemble of NMR structures), interhelical hydrogen bonds, and native contacts separat

149 -META II transition and the rearrangement of interhelical hydrogen bonds.

150 association through a cooperative network of interhelical hydrogen bonds.

151 so leads to the formation of new stabilizing interhelical hydrogen-bond contacts, such as those betwe

152 ed in a right-handed bundle held together by interhelical hydrophobic interactions similar to the str

153 Structural modeling predicts an interhelical hydrophobic interface between paired EF han

154 er, we have explored the role of a potential interhelical interaction between an Arg at an interior d

155 ders both the adjacent Trp126 and a critical interhelical interaction between transmembrane III (TM I

156 MCP exhibits an equally strong interhelical interaction in the TM domain.

157 these findings indicate that differences in interhelical interaction regulate the different activati

158 Our data provide insight into a critical interhelical interaction required for NIS folding and ac

159 at Met257 may form an important and specific interhelical interaction with a highly conserved NPXXY m

160 dual role for Asp(2.61(98)): formation of an interhelical interaction with Lys(3.32(121)) that contri

161 orrelates well with the stability of the N-C interhelical interaction.

162 ot share this pattern of diverse polar-polar interhelical interaction.

163 f two dynamic helices that are stabilized by interhelical interactions and are connected by a short l

164 ified triplets that have high propensity for interhelical interactions and are unique to membrane pro

165 cal beta-oligomers stabilized via long-range interhelical interactions and stapled together by a disu

166 Unusual interhelical interactions are mediated by a series of co

167 wo differently labeled proteins representing interhelical interactions are observed.

168 tic side-chains contribute to the presumably interhelical interactions between gamma(6) TM1 and the C

169 To assess specific interhelical interactions between residues, we have deve

170 residues identified here modulate important interhelical interactions between the fifth and sixth tr

171 Combined, these localized defects in interhelical interactions cause structural changes that

172 equences of the OX receptors that affect key interhelical interactions formed between TM3 and neighbo

173 tion model has been developed to predict the interhelical interactions in alpha-helical membrane prot

174 imers, resulting from unfavorable intra- and interhelical interactions in the interfacial coiled coil

175 To elucidate the nature of interhelical interactions in this heptahelical receptor

176 estimated the relative strength of the five interhelical interactions of apoLp-III.

177 e we investigate the role of these conserved interhelical interactions on the structure and function

178 ture is stabilized primarily by hydrophobic, interhelical interactions, and several critical contacts

179 residue pairs that have high propensity for interhelical interactions, but disulfide bonds are rarel

180 bilization of rHDL by specific electrostatic interhelical interactions, in agreement with the double

181 However, kinks invariably affect numerous interhelical interactions, questioning the acceptance of

182 the P22 subdomain is stabilized by tertiary interhelical interactions.

183 of glutamines engage in extensive intra- and interhelical interactions.

184 the receptor, was analyzed in the context of interhelical interactions.

185 esting involvement of R160/H162 in important interhelical interactions.

186 th the retinal binding pocket and stabilizes interhelical interactions.

187 to have correlated behavior in participating interhelical interactions.

188 Salt bridges provide specificity in interhelical interactions.

189 ns the TRH-R in an inactive conformation via interhelical interactions.

190 Residues at positions a and d form the interhelical interface and are usually hydrophobic.

191 coiled coil by residues that destabilize the interhelical interface, such as Ala clusters, is require

192 x of the seven conserved Gly residues at the interhelical interface.

193 tad repeat, with two aspartate-lysine (d-g') interhelical ion pairs in the symmetrical dimer.

194 ized in a synthetic sequence by replacing an interhelical ionic bond with a covalent bond.

195 c NHR-trimer, by incorporating site-specific interhelical isopeptide bonds as the redox-sensitive dis

196 are used to superimpose helix H1 of a target interhelical junction onto the corresponding iH1 of the

197 The simplest RNA dynamical unit is a two-way interhelical junction.

198 formation, specific ion-binding, and complex interhelical junctions present in prior studies preclude

199 largely determined by the topology of their interhelical junctions.

200 served Ala residue at position 75 forming an interhelical kink.

201 labeling with [(14)C]halothane suggested an interhelical location of halothane with a stoichiometry

202 riggered by a loop-to-helix transition of an interhelical loop (B loop) within the fusion domain and

203 the distance between a spin label on the EF interhelical loop and a label on either the AB or the CD

204 In order to study the role of the interhelical loop conformations in the structure and fun

205 his C-terminal extension is homologous to an interhelical loop found in several membrane proteins, in

206 Site-specific cleavage on the interhelical loop I on the cytoplasmic face of rhodopsin

207 suggesting that the relative motion between interhelical loop I-II and helix VII is not crucial for

208 e prepared: disulfide bond 1, between Cys65 (interhelical loop I-II) and Cys316 (end of helix VII); d

209 355) that comprise the TH8 helix and the TL5 interhelical loop in the native toxin.

210 mobility analysis of spin-labels on the B-C interhelical loop indicates that the antiparallel beta-s

211 n-labeled side chains indicates that the E-F interhelical loop is largely alpha-helical, being formed

212 n interactions with target peptides restrain interhelical loop motions, acting to tune the conformati

213 The conformation of the structured EF interhelical loop of bacteriorhodopsin and its change in

214 P fused to the 25 amino acids comprising the interhelical loop of PI-10 (i.e. Arg-63 to Glu-87), wher

215 boundary between helix 4 and the subsequent interhelical loop resulted in large changes to the stabi

216 Mutation of four basic amino acids in the interhelical loop to alanines (i.e. K74A, K75A, R76A, K7

217 loop and a label on either the AB or the CD interhelical loop were observed, and the changes were mo

218 This sequence includes the E-F interhelical loop, a transducin interaction site.

219 position in the sequence S240-V250 in the EF interhelical loop, at position 65 in the AB interhelical

220 interhelical loop, at position 65 in the AB interhelical loop, or at position 140 in the CD interhel

221 e ligand binding properties of the conserved interhelical loop, the only portion of the protein expos

222 nstruct encoding EGFP coupled to the mutated interhelical loop.

223 induced conformational alteration of the F-G interhelical loop.

224 erhelical loop, or at position 140 in the CD interhelical loop.

225 e partially disordered N-terminal region and interhelical loop.

226 well beyond the bilayer, with a well-defined interhelical loop.

227 rmuted by introducing new chain termini into interhelical loops and by constraining the N- and C-term

228 nformational gating mechanism, involving two interhelical loops and one alpha-helix of GLTP, could en

229 Interestingly, the interhelical loops are, on average, actually more conser

230 ctions of the four aspartic acid residues in interhelical loops at the cytoplasmic surface of bacteri

231 The distance between the C-D loop and E-F interhelical loops in A103R1/M163R1 increased approximat

232 Residue differences within interhelical loops may account for the contrasted thermo

233 ineered cysteine residues in the cytoplasmic interhelical loops of bR.

234 its were constructed by exchanging the S5-S6 interhelical loops of each domain between hH1 and hSkM1

235 formational alterations in the HinRs and the interhelical loops of luteinizing hormone receptor/folli

236 The interhelical loops of six to seven of the c subunits are

237 s were generated to predicted helices and/or interhelical loops of SP-B and tested for fusion, lytic,

238 In the presence of ubiquitin, two interhelical loops of the C-terminal four-helix bundle a

239 anonical secondary structures with shortened interhelical loops that disrupt the conserved tRNA terti

240 nd dynamic studies suggest that two flexible interhelical loops, the flexible C-terminal tail, and on

241 nd cleavage of the major PS1 polypeptides in interhelical loops.

242 -labeled fragments that contain all internal interhelical loops.

243 rd their cytoplasmic sides as well as in the interhelical loops.

244 n arises from the freezing out of collective interhelical motional modes.

245 J2a/b is intrinsically flexible but the interhelical motions across the loop are remarkably rest

246 a) and displays large amplitude, anisotropic interhelical motions characterized by a 0.52(+/-0.04) in

247 opology of the core domain, and suggest that interhelical motions in P2ab facilitate nucleotide addit

248 Interhelical motions were studied under different ionic

249 onstrated that imposing such restrictions on interhelical movement can change the hammerhead ribozyme

250 lves large-scale reorganization of the H3/H6 interhelical network.

251 obs-into-holes packing with a characteristic interhelical offset of 0.25 heptad.

252 spin relaxation and drive global changes in interhelical orientation.

253 e characterize this transition, with various interhelical orientations, by pseudodihedral angle and i

254 tions that destabilize the N- and C-terminal interhelical packing interactions also reduce viral infe

255 Our results strongly suggest that conserved interhelical packing interactions in the F protein fusio

256 sults provide strong evidence that conserved interhelical packing interactions in the gp41 core are i

257 r; (ii) loss of alpha-helicity and decreased interhelical packing interactions in transmembrane regio

258 ptimization of Calpha-H hydrogen bonding and interhelical packing is sufficient to computationally pr

259 form a one-dimensional lattice with distinct interhelical packing regimes.

260 smatches in the receptor helix, enabling RNA interhelical packing through specific recognition of Wat

261 tly packed DHA molecules tends to weaken the interhelical packing.

262 phospholipid showed low flexibility and good interhelical packing.

263 Small-residue-mediated interhelical packings are ubiquitously found in helical

264 oluble protein have the same distribution of interhelical pairwise propensity.

265 A buried interhelical polar interaction between two Asn residues

266 y residue pairs have high propensity to form interhelical polar-polar atomic contacts, for example, r

267 The interhelical positions of the conserved strong H-bonds a

268 lation between amino acid packing values and interhelical propensity, we propose the concept of a hel

269 ned by protein-lipid interactions instead of interhelical protein-protein interactions, and the S4 am

270 vorable interactions with side chains in the interhelical region and form a persistent hydrogen-bond

271 interaction requires K2, part of K3, and the interhelical region between K1 and K2.

272 derived from the second and third exofacial, interhelical regions of band 3 completely inhibited the

273 k, relocating spermine from major grooves to interhelical regions, thereby increasing DNA-DNA attract

274 sting largely of the Euler angles describing interhelical reorientation.

275 However, interhelical rotational resonance measurements between 1

276 be initiated by disruption of a constraining interhelical salt bridge ().

277 Disruption of an interhelical salt bridge between the retinal protonated

278 chanism between BR and SRII appears to be an interhelical salt bridge locked conformational switch th

279 The unusual absence of interhelical salt bridges here exposes apolar core atoms

280 ide chains of VBP, which is also involved in interhelical salt bridges in the leucine zipper.

281 he phospholipid to solvent; a lack of buried interhelical salt bridges in the terminal domains correl

282 at are thought to form extensive stabilizing interhelical salt bridges.

283 stulated to be initiated by disruption of an interhelical salt-bridge constraint between an aspartic

284 Instead, interior charged residues form interhelical salt-bridges with residues at the adjacent

285 rallel helical rings rotationally aligned by interhelical salt-bridges.

286 he helix I-IV interface and the helix II-III interhelical segment or in helices III and IV of the NaV

287 We measured the N-terminal interhelical separation of the BM2 channel using fluorin

288 y interlaced long-range interactions between interhelical sequences.

289 ysis, that the average contribution of eight interhelical side-chain hydrogen-bonding interactions th

290 mobile N-terminal segment of SDF-1alpha with interhelical sites of the receptor, resulting in a biolo

291 are: 1) predicted to be oriented toward the interhelical space; 2) analogous to those required for l

292 X-ray scattering measurements of interhelical spacing in these sheets support a tight rid

293 The interhelical subdomain is at least partially buried and

294 d eight-helix bundle organized by a specific interhelical TCR TM interface.

295 otif and its two peptide fragments show that interhelical tertiary contacts are critical for stabiliz

296 We introduce the concept of interhelical three-body interactions as derived from Del

297 with protein stability, we found that tight interhelical triplet interactions exist extensively in o

298 g directed evolution to optimize an inserted interhelical turn.

299 res and sequence preferences of two types of interhelical turns, each of which connects the two helic

300 ned for the Lpp-56 coiled coil suggests that interhelical van der Waals interactions are disrupted in