ライフサイエンスコーパス: internal standard

1 t, such as Cr(acac)3, and CH3(13)COONa as an internal standard.

2 Monitoring mode using labeled glutathione as internal standard.

3 ith respect to thorium, considering it as an internal standard.

4 peptide using stable isotope labeled peptide internal standard.

5 ) in the positive ion mode with DMA D6 as an internal standard.

6 sera, using a non-human cel-miR-39-3p as an internal standard.

7 e introduced as a conceptually new source of internal standard.

8 shed along with the acceptable range for the internal standard.

9 S/MS quantification using genistein-d4 as an internal standard.

10 achieved while using an isotopically labeled internal standard.

11 The quantification of the PAHs utilised an internal standard.

12 e analyte do contribute to the signal of the internal standard.

13 study, is corrected by referencing versus an internal standard.

14 ic tryptic peptides of UCH-L1 was used as an internal standard.

15 tized (13)C-labeled keto acid was used as an internal standard.

16 ture of just alpha-pinene and benzene as the internal standard.

17 cision using potassium hydrogen phthalate as internal standard.

18 3, and 0.91 V versus a ferrocene/ferrocenium internal standard.

19 -carboxyfluorescein diacetate (6-CFDA) as an internal standard.

20 cted with yeast enolase added to serum as an internal standard.

21 ) with (15)N-labeled full-length apoE4 as an internal standard.

22 otted onto a tissue section using deuterated internal standard.

23 g the relative ratio between the analyte and internal standard.

24 was achieved by using 2,5-hexanedione as an internal standard.

25 dard, and a spiked lanthanide as a secondary internal standard.

26 d by normalizing signal intensities using an internal standard.

27 17beta-estradiol-D5 was used as internal standard.

28 ilution using mass-labeled 1,4-dioxane-d8 as internal standard.

29 Dihydroisosteviol was used as an internal standard.

30 iment using isotope labeled amitriptyline as internal standard.

31 known concentration of propranolol-d7 as an internal standard.

32 mode with deuterium-labeled vitamin K1 as an internal standard.

33 e calibration curves and sulfanilamide as an internal standard.

34 ded on mass spectrometry and isotope-labeled internal standards.

35 ssues, and quantified relative to deuterated internal standards.

36 conducts harmonization according to its own internal standards.

37 raditional methods of standard additions and internal standards.

38 olutions of D4/D5 and (13)C-labeled D4/D5 as internal standards.

39 evaluate the normalization potential of the internal standards.

40 PCB 155 and PCB 198 were employed as internal standards.

41 each barrel was continuously monitored with internal standards.

42 ated propane and butane generated in situ as internal standards.

43 is not possible using the traditional acidic internal standards.

44 (r(2)) greater than 0.99 without the use of internal standards.

45 parallel due to the need for isotope labeled internal standards.

46 ix-matched standards or isotopically labeled internal standards.

47 ynthetic peptides versus QconCAT peptides as internal standards.

48 ratiometric comparisons with stable isotope internal standards.

49 like LC-MS with isotope-labeled peptides as internal standards.

50 res employing LC/MS and isotopically labeled internal standards.

51 amples and quantification without the use of internal standards.

52 (methanol-dichloromethane) with appropriate internal standards.

53 hased commercially or synthesised for use as internal standards.

54 corresponding deuterium-labeled analogues as internal standards.

55 re affected by the choice of calibration and internal standards.

56 using deuterated analogues of these drugs as internal standards.

57 biomass and used them as tracers ((15)N) and internal standards ((13)C).

58 0.98 over about 3 orders of magnitude if an internal standard (15:0 FFA) was added.

59 n in a single experimental run with a single internal standard, (15)N-apoE4.

60 eum biomarkers, notably including the native internal standard 17alpha(H),21beta(H)-hopane, commonly

61 (24R),25(OH)2D3 and added deuterated labeled internal standard (24R),25(OH)2D3-d6 were extracted from

62 When using green fluorescent protein as an internal standard, 77K fluorescence emission spectra on

63 l in pooled human plasma with ramipril as an internal standard, a greater than 4-fold improvement in

64 iplex Absolute Regressed Quantification with Internal Standards-a novel mass spectrometry-based appro

65 Quality controls based on the results of internal standards added at the samples' extraction, dil

66 nstrated that PRM with SILAC histones as the internal standards allowed reproducible measurements of

67 Sin a 1, used as an internal standard, allowed quantification of this mustar

68 using its isotopically labelled analogue as internal standard, allowing to assess the protein conten

69 he analysis was investigated: sample amount, internal standard amount, and sample dilution factor.

70 n the traditional approach which utilizes an internal standard and the multiplexing approach which re

71 re is no "cross talk" between signals of the internal standard and the target analyte.

72 n-Propanol was used as an internal standard and the three carboxylate peaks were r

73 of multiple sources of information including internal standards and clustered chromatograms in a math

74 thods coupled with the use of stable isotope internal standards and either liquid chromatography or g

75 and highlights the need for use of suitable internal standards and isotope dilution techniques.

76 es and is only quantitative when compared to internal standards and reference cell lines.

77 mogenized with isotopically labeled analogue internal standards and sampled using gas-phase SPME.

78 by tandem mass spectrometry using synthetic internal standards and selected reaction monitoring.

79 n were achieved using stable isotope-labeled internal standards and tandem mass spectrometry.

80 n of 4-6%, stable migration times (1.4% with internal standard), and capillary recoveries on the orde

81 DHB as MALDI matrix, a primary lipids-based internal standard, and a spiked lanthanide as a secondar

82 The Au core of the Au@Ag NPs acts as an internal standard, and aids in the analysis of the compl

83 served peptides, a heavy isotope labeled mAb internal standard, and anti-human Fc enrichment.

84 ion based on the Folch method, with tocol as internal standard, and normal-phase HPLC with fluorescen

85 f the fragment ions from the analyte and its internal standard, and the ratio is used in quantitative

86 which allows control cells to be used as an internal standard, and thus we could detect shRNAs with

87 rget fractions via on-line SRM monitoring of internal standards, and fraction multiplexing before nan

88 quantification can be achieved without using internal standards, and integrated peak areas based on t

89 astereomeric mixture of isotopically labeled internal standards, and multiple reaction monitoring mas

90 nsured the reproducibility of the matrix and internal standards application with RSD of 10-15%.

91 s for using isotopically labeled rhGH as the internal standard are described.

92 n each laser shot, even when the analyte and internal standard are quite disparate in m/z, thereby im

93 In the present work, isotopically labeled internal standards are applied to tissue sections to max

94 Stable isotope-labeled internal standards are of great utility in providing acc

95 tical standards, including deuterium-labeled internal standards, are also described.

96 situation where an analyte is present in the internal standard as an impurity.

97 sitivity, and allows addition of an adequate internal standard at the clinical lab prior to analysis,

98 trapolation to the nominal temperature, (vi) internal standard-based correction, and (vii) simple rec

99 mogenization is traced by the addition of an internal standard before storage.

100 To address these problems, we developed Internal Standard Calibrated ChIP (ICeChIP), wherein a n

101 The method performance evaluated with internal standard calibration method produced good analy

102 We demonstrate for the first time that this 'internal standard' calibration method satisfies the sine

103 Single-point internal standard calibrations were used to estimate abs

104 oximately 1 muM, and calibration curves with internal standards can achieve a precision of 1-9% withi

105 The internal standard capillary electrophoresis method (IS-C

106 ; AOAC, 991.41, 1995; AOAC 998.12, 2005) and Internal Standard Carbon Isotope Ratio Analysis could no

107 hat multiple reaction monitoring assay using internal standards carrying peptides for quantification

108 e (COC) from brain tissue using a deuterated internal standard (COC-d(3)) combined with wide-isolatio

109 s determined experimentally for each analyte/internal standard combination and an adjustable calibrat

110 lso allows for testing of different analytes/internal standard combinations, which helps with the sel

111 Seven were quantified using an internal standard combined with external calibration cur

112 To test how well the internal standards compensate for differences in tissue

113 atio on the y-axis versus the inverse of the internal standard concentration on the x-axis.

114 weight compounds, and those at high analyte/internal standard concentration ratio.

115 type (H(+), Na(+), and K(+)) for analyte and internal standard corrected for different ionization eff

116 Normalization to internal standards corrected a 2-fold difference in lipi

117 , were 91-121% and 90-113% (without and with internal standard correction, respectively), for wines f

118 led peptide, a cleavable SIL peptide, and an internal standard created by differential derivatization

119 onventional relative quantification using an internal standard curve and need for calibrant diluent,

120 avy-labelled standard peptides, to construct internal standard curves for peptides derived from key n

121 Internal standards DA-d4 and DOPAC-d5 result in standard

122 17 or -1.08 V versus a ferrocene/ferrocenium internal standard depending on the type of functionaliza

123 euterated derivative 1b is of interest as an internal standard during MC quantification in biological

124 We demonstrate that the presence of internal standard enables online quantification of analy

125 rds [(15)N5]dG-gx-dC and [(15)N5]dG-gx-dA as internal standards, enzyme hydrolysis to release the cro

126 Prior to optimization, internal standards exhibited strong matrix effects with

127 When pH-insensitive AuNCs were used as an internal standard, FITC/BSA-AuNCs offered a sensitive an

128 uterated tricin analog was synthesized as an internal standard for accurate quantitation purposes.

129 over 3 days while using a single nonisotope internal standard for data normalization.

130 sed method can be utilized to generate novel internal standard for isotope dilution and to extend the

131 methods of applying an isotopically labeled internal standard for MALDI IMS analysis.

132 lts clearly demonstrate the necessity for an internal standard for quantitative MSI experiments.

133 We conclude that the best internal standard for the workflows both with and withou

134 omparable and (2) nine glycolytic enzymes as internal standards for a more accurate calculation of re

135 r, isotopic labeled substances are needed as internal standards for a reliable and convenient quantif

136 rement and the use of stable isotope-labeled internal standards for absolute quantitation.

137 Fully (13)C-labeled sugars are used as internal standards for composition analysis.

138 of lipid species in biological tissues using internal standards for each lipid class.

139 ction linked to the recovery of mass-labeled internal standards for every analyte within every sample

140 In this work, we synthesized internal standards for four garlic organosulfur compound

141 hloride was used to generate isotope-labeled internal standards for improved quantification.

142 ) as a derivatizing agent and polydeuterated internal standards for maximum accuracy and precision.

143 that employed stable isotope-labeled peptide internal standards for quantitation of HMPV.

144 wave-assisted deuterium exchange, for use as internal standards for stable isotope dilution analysis.

145 Internal standards for the absolute quantification of th

146 ration, thus facilitating the preparation of internal standards for the accurate quantification of en

147 )) by using solid phase peptide synthesis as internal standards for the CIEF immunoassay.

148 using isotopically labeled (IL) analogues as internal standards for the target analytes are required

149 Transferrin levels obtained by both internal standards give overlapping, or nearly overlappi

150 The utilization of (13)C internal standards greatly improved the accuracy and rel

151 plied to vanillin with dimethylsulfone as an internal standard, has an equivalent accuracy (<1 per th

152 Internal standards have been introduced in quantitative

153 DON-d1 was used as internal standard (I.S.) which helped to compensate the

154 t using stable isotope labeled (SIL)-protein internal standard (I.S.).

155 approaches utilizing various calibrators and internal standards (I.S.) have been developed.

156 characteristics were evaluated for different internal standards, i.e., PEGylated and polyhistidine-ta

157 The PICALM QconCAT was first used as an internal standard in a multiple reaction monitoring assa

158 sample and so transfer both the analyte and internal standard in a single fixed volume onto a substr

159 eference of analyte intensity to that of the internal standard in each laser shot, even when the anal

160 peak area ratios of the analytes versus the internal standard in five repetitive analyses over appro

161 oalanine and (13)C labeled BCA is used as an internal standard in gammaGBCA analysis.

162 flow injection analysis (FIA)-ICPMS serve as internal standard in nanoHPLC-ESI-MS/MS.

163 xperiments further exemplify the need for an internal standard in the MSI workflow.

164 mall volumes of sample, where addition of an internal standard in the sample is either troublesome or

165 molecule propranolol and its isotope labeled internal standard in water, unprocessed river water and

166 ries and provide an example for their use as internal standards in the profiling and absolute quantif

167 eference procedure were: (1) use of EC-d5 as internal standard instead of less similar propyl carbama

168 By spiking an isotopically labeled internal standard into the plasma sample, a linear calib

169 marker analysis provided that an appropriate internal standard is used and that readouts are monitore

170 )C NMR, which can even be misleading when no internal standard is used as in the above-mentioned pape

171 t time, a polymeric (13)C lignin was used as internal standard (IS) for lignin quantification via ana

172 re prepared by micropipetting standards with internal standard (IS) on top of the tissues, and averag

173 SRM (ICSRM) of the proposed SIL drug and SIL internal standard (IS) was theoretically calculated to g

174 acids (FAAs) in rice using l-theanine as the internal standard (IS) with o-phthalaldehyde (OPA) reage

175 concerning the extraction process, usage of internal standard (IS), and influences on the ionization

176 ble isotope labeled signature peptide as the internal standard (IS).

177 )) in human plasma using dapagliflozin as an internal standard (IS).

178 In this study, we fabricated standards and internal standards (ISs) for absolute quantification via

179 ive LC-MS/MS typically relies on addition of internal standards (ISs).

180 This work proposes to integrate an internal standard (ISTD) into the microchip by adding it

181 using [(13)C2, (2)H3]-labeled isotopologs as internal standards; (iv) sequencing paCOS by tandem MS.

182 ples are not completely represented in these internal standards, leading to orphan analytes for which

183 aches using a matrix-adapted calibration, an internal standard material and a modified standard addit

184 powerful tool for the generation of valuable internal standard materials for LC-MS/MS analyses highly

185 e also showed that the quantification by the internal standard method can be done on the low-field NM

186 This internal standard method proved to be more accurate than

187 and GCMS analyses as well as quantitation by internal standard method showed that (Z)-3-hexenal, ethy

188 gn and implementation of a coinjected triple-internal standard method to alleviate the need of an acc

189 Quantification was performed by the internal standard method using a five points regression

190 the response factors approach along with the internal standard method, allowing for quantification of

191 xternal calibration, standard additions, and internal standard methods using ICP OES.

192 An isotopically labeled internal standard mix was incorporated into the extracti

193 les and a pooled sample that serves a global internal standard, mixing of the isotope differentially

194 aser energy and varied the amounts of matrix-internal standards mixture to evaluate the normalization

195 A post-column continuous infusion of internal standard (nigericin) with matrix-matched calibr

196 Internal standard-normalized data allowed intrasample mo

197 n chemistry using d0/d3-labelled derivatized internal standards obtained by simple reaction procedure

198 of the 11 monoterpenes, monitored versus an internal standard of benzene, indicated stability in the

199 one point gravimetric standard addition with internal standard of the same (29)Si spike was employed

200 , either as an absolute value or relative to internal standards of known density.

201 quantification, (15)N-metabolically labeled internal standards of rhTRAIL(WT) and rhTRAIL(4C7) were

202 derate matrix content, the use of an optimum internal standard (OIS) at a concentration close to that

203 ers, including comigration with a deuterated internal standard or in silico prediction of electromigr

204 concentration without the use of calibrants, internal standards, or a calibration curve.

205 antitation that does not rely on calibrants, internal standards, or calibration curves but, rather, l

206 pport the use of recombinant SIL proteins as internal standards over SIL peptides due to their abilit

207 Isotopically labeled internal standards, Paraquat-D6 and Diquat-D4, were used

208 a novel strategy using a postcolumn infused-internal standard (PCI-IS) method with liquid chromatogr

209 onization using postcolumn infusion of eight internal standards (PCI-IS).

210 g/mL) generated by dividing MMC peak area by internal standard peak area and plotting the area ratio

211 erformed with peak areas, ratios of standard/internal standard peak area, and concentrations.

212 also have implications for the selection of internal standards, peaks used for internal mass calibra

213 Moreover, the use of isotope labeled internal standards permitted the quantification of the c

214 We evaluated three conventional internal standard platforms (synthetic peptides, QconCAT

215 ed to assess quantitative performance of the internal standard platforms.

216 Instead of relying on an internal standard polymer, the intensities of 40 poly(8:

217 is determined relative to a perchlorate-ion internal standard, preloaded into the vesicle along with

218 ked with (2)H isotope-labelled N-nitrosamine internal standard prior to extraction.

219 spectrometry, utilizing a specially designed internal standard procedure.

220 Comparing fingerprinting with and without internal standard proved that the presence of the (13)C

221 atched calibration method using carvacrol as internal standard, providing quantification limits betwe

222 d by up to 90%; however, when the analyte-to-internal-standard ratio was monitored, these differences

223 By monitoring the analyte-to-internal-standard ratio, differences in ion signal were

224 The addition of internal standard reduced the coefficient of variation f

225 nd QD red emission band that functions as an internal standard requiring only a single excitation wav

226 -98.3-47.0% and -98.0-67.2% for analytes and internal standards, respectively.

227 cts, good RRs are obtained regardless of the internal standard selected.

228 contributions from analyte to stable labeled internal standard signal exist.

229 are used to prepare a plot of the analyte-to-internal standard signal ratio on the y-axis versus the

230 e results showed, even after correction with internal standard, significantly lower intensities from

231 with known amounts of stable-isotope-labeled internal standards (SIL-IS) allows measurements of the c

232 l ions were redispersed in a small volume of internal standard solution and deposited onto a quartz r

233 e during this process and the volumes of the internal standard solution and sample are both fixed by

234 addition of (34)S and a generic S-containing internal standard spiked to the sample provides full com

235 metabolomic experiment; and (2) mixtures of internal standards spiked in plasma samples at varying c

236 icle describes the evaluation of a practical internal standard spray addition technique, used prior t

237 Separation resolution between the internal standard, substrate, and product is 1.2 and ave

238 without the use of liquid chromatography or internal standards such as chemical or metalized labels.

239 is mainly due to the limited availability of internal standards that can adequately correct for varia

240 Using bis(2-ethylhexyl) sebacate as an internal standard, the kinetics of the ozone-BeP reactio

241 ncluding the amount of peptide substrate and internal standard, the timing of cleavage reaction, and

242 derivatization and a stable isotope labeled internal standard, the total retronecine-/heliotridine-t

243 t prior consideration of R TS and the use of internal standards, the relative increase in GFP calcula

244 f a sampling glass capillary was coated with internal standard then used to draw liquid sample and so

245 The phosphate backbone signal was used as an internal standard to calibrate the absolute signal of ea

246 he synthesized desmosine-d(4) is used as the internal standard to develop an accurate and sensitive i

247 Dantron was used as an internal standard to ensure accuracy and reproducibility

248 otope-labeled full-length standard CD4 as an internal standard to measure endogenous CD4 directly, wi

249 The use of a internal standard to normalize regional differences in i

250 euterium-labeled cholesterol was used as the internal standard to obtain the absolute quantity of the

251 abeled full-length apoE4 ((15)N-apoE4) as an internal standard to quantify concentrations of the spec

252 eptides with fast HDX rates is introduced as internal standards to adjust the variations in the exten

253 thod based on application of matrix and dual internal standards to allow intra- and intersample norma

254 ll-E-beta-apo-8'-carotenoic acid ethyl ester internal standards to compensate for analytical variatio

255 eparate the three metabolites, (ii) isotopic internal standards to correct for in-source pGlu formati

256 enone, d3-skatole and d6-indole were used as internal standards to eliminate matrix effects.

257 -labeled analogues were prepared and used as internal standards to identify and quantify these charac

258 ino acids on C- and N-terminals were used as internal standards to minimize the variability during sa

259 floxacin and ciprofloxacin - d8 were used as internal standards to quantify the (fluoro)quinolones.

260 Two deuterium-labeled internal standards (triply deuterated GHRP-4 and GHRP-2

261 The quest for internal standards useful in MALDI imaging studies goes

262 lozapine, and buspirone and their deuterated internal standards using the matrix 3-nitrobenzonitrile

263 An internal standard was added to the mixture after stress,

264 The internal standard was automatically mixed into the sampl

265 uantification method using ((2)H2)GABA as an internal standard was developed and validated according

266 nnose, fructose and ribose) plus inositol as internal standard was obtained in 500mmol.L(-1) triethyl

267 meant that quantitative analysis without an internal standard was possible.

268 on assay (SIDA) with d7-gamma-decalactone as internal standard was used for quantitative analysis of

269 able isotope dilution using creatinine-d3 as internal standard was used for quantitative measurements

270 In this study, a deuterated internal standard was used to account for these signal v

271 A fully carbon-13-labelled internal standard was used to compensate for matrix effe

272 AI determined for five consecutive days with internal standards was better than 20% using manual samp

273 ors and stable isotope labeled (SIL) peptide internal standards was validated for human liver.

274 rfaces by DBDI-MS, using TATP-3,6,9-(13)C as internal standard, was done and validated using an LC/AP

275 By using an internal standard, we are able to quantify the density o

276 Using DART-MS/MS and isotopically labeled internal standards, we achieved detection limits of 21 n

277 Using deuterated homologues as internal standards, we observed limited and relatively s

278 Using a preformulated mixture of eight internal standards, we quantified the glycans in a monoc

279 Through the use of added internal standards, we successfully determined the conce

280 The analytes and internal standard were extracted from human plasma using

281 Deuterium labelled Vit D and 25OH-Vit D internal standards were added as surrogates prior to sap

282 Analogue and stable-isotope-labeled (SIL) internal standards were evaluated including an in-house

283 Quantification concatamer (QconCAT) internal standards were originally designed for a large-

284 rominated standards and isotopically labeled internal standards were synthesized and purified to conf

285 Internal standards were synthesized for internal absolut

286 ic reference standards and deuterium-labeled internal standards were synthesized for use in an assay

287 HPLC and internal standards were synthesized using Grignard addit

288 Deuterium-labeled internal standards were then mixed with the CS-derived d

289 The representative AHL internal standards were then used to develop an optimize

290 Isotope-labeled internal standards were used for all analytes.

291 vant levels of dA M+1 (0.25-20%) and dA M+5 (internal standard) were used for sample quantitation.

292 including a [(13)C]-labeled version used as internal standard, were immuno-enriched from rat serum w

293 m the shell, and a probe molecule as a Raman internal standard, were rationally designed for quantita

294 ibrators) and external calibration (i.e., as internal standards) when quantifying three forms of unla

295 etabolite standards and isotopically labeled internal standards, whereas the corresponding mitochondr

296 analysis with fully (13)C-labeled biomass as internal standard, which is generated by growing E. coli

297 g/mL was achieved by employing (15)N-IGF1 as internal standard, which proved to be an essential featu

298 The gQD/Cy3 FRET pair served as an internal standard, while the rQD/A647 FRET pair served a

299 established utilizing stable isotope peptide internal standards with liquid chromatography-tandem mas

300 lity permitted kcat/km determination without internal standards with observed values ~10x improved ve