ライフサイエンスコーパス: intersectin

1 r endocytic adaptor, also binds to Eps15 and intersectin.

2 Eps-homology (EH) domain proteins Eps15 and intersectin.

3 ) via interactions with the scaffold protein intersectin.

4 enerated strong binding to the EH domains of Intersectin.

5 e C terminus of RAB/Rip to the EH domains of Intersectin.

6 entified the multidomain scaffolding protein intersectin 1 (ITSN1) as an important regulator of this

7 e report that loss of the signaling scaffold intersectin 1 (ITSN1) in mice leads to defective neurona

8 Intersectin 1 (ITSN1) is a scaffold protein that regulat

9 The multidomain scaffolding protein intersectin 1 (ITSN1) is an important regulator of this

10 These phenotypes were rescued by wild-type intersectin 1 but not by a locked mutant of intersectin

11 Mutations in unc-11 (AP180) or itsn-1 (Intersectin 1), which reduce clathrin-dependent endocyto

12 a multi-modular protein, Cin1 (cryptococcal intersectin 1), whose domain structure is similar to tha

13 th the Src homology 3 domain-bearing protein Intersectin 1, an intracellular protein involved in syna

14 ts of SCAMP1 bind to two EH domain proteins, intersectin 1, which is involved in endocytic budding at

15 intersectin 1 but not by a locked mutant of intersectin 1.

16 regulated by an intramolecular switch within intersectin 1.

17 ocytic machinery proteins Eps15, Eps15R, and intersectin 1.

18 Eps15 homology domains of Eps15, Eps15R, and intersectin 1.

19 Deletion of intersectin 1/2 in mice alters the presynaptic nanoscale

20 release from infected cells, as the lack of intersectin-1 recruitment leads to a loss of Cdc42 activ

21 th the Epsin15 homology domains of Eps15 and intersectin-1.

22 ous nuclear ribonucleoprotein H', Br140, and intersectin-1.

23 ssion of exchange activity suggests that the intersectin 1L exchange activity is regulated by endocyt

24 Intersectin 1L is a scaffolding protein involved in endo

25 by an intramolecular interaction between the intersectin 1L SH3 domain region and the adjacent DH dom

26 We demonstrate that the intersectin 1L SH3 domains, which bind endocytic protein

27 controlling the actin nucleation activity of intersectin 1L.

28 Herein, we investigated whether intersectin-1s (ITSN) deficiency and prolonged lung expr

29 We now show that a decrease in intersectin-1s (ITSN-1s) expression due to granzyme B (G

30 Here we addressed the role of intersectin-2L (ITSN-2L), a guanine nucleotide exchange

31 Intersectin, a multiple Eps15 homology and Src homology

32 160 kDa (dap160), the Drosophila homolog of intersectin, a putative adaptor for proteins involved in

33 ts with the SH3A (Src homology 3A) domain of intersectin, an endocytic-exocytic adaptor protein.

34 GEF activity afforded by DH/PH fragments of intersectin and Dbs are also not altered by phosphoinosi

35 While the PH domains of intersectin and Dbs promiscuously bind several multiphos

36 We used dominant-negative intersectin and dynamin constructs to show that SGK1-med

37 t EHSH1 is very similar to Xenopus and human intersectins and to human SH3P17.

38 ytic machinery, including dynamin and Dap160/intersectin, and negatively regulates retrograde BMP gro

39 Our data reveal intersectin as an autoinhibited scaffold that serves as

40 y the endocytic multidomain scaffold protein intersectin as an important regulator of SV replenishmen

41 ed protein 160 (Dap160; related to mammalian intersectin) as an aPKC-interacting protein in Drosophil

42 ) immunogold labeling studies indicated that intersectin associated preferentially with the caveolar

43 We named this protein Intersectin, because it potentially brings together EH a

44 /Rip) and two novel proteins, which we named Intersectin-binding proteins (Ibps) 1 and 2.

45 rotein, the GTPase Cdc42, and its activator, Intersectin, biochemically and by solving the crystal st

46 r example, SH3 domains from c-Src, Grb2, and intersectin bound only to the C-terminal half of dynamin

47 pha Gpa1, protein kinase Pkc1, and endocytic intersectin Cin1.

48 otide exchange activities of these synthetic Intersectin constructs can be controlled in a rapid and

49 Second, intersectin cooperated with epidermal growth factor to p

50 And finally, intersectin cooperated with progesterone to accelerate m

51 mains of three distinct Dbl family proteins, intersectin, Dbs, and Tiam1, selectively bind lipid vesi

52 We found that intersectin directly associates with synapsin I through

53 al data reveals that the Dbl exchange factor intersectin engages a strictly conserved GTPase residue

54 adaptor complex composed of FCHO, Eps15, and intersectin, ESCRT-0 accumulation at the cell surface is

55 Third, intersectin expression was sufficient to induce oncogeni

56 A cell-free system depleted of intersectin failed to support caveolae fission from the

57 Indeed, intersectin functions in the intermediate stages of clat

58 t, we provide several lines of evidence that intersectin has the capacity to activate mitogenic signa

59 election experiments with the SH3 domains of Intersectin identified two endocytic proteins, dynamin a

60 er, the results indicate the crucial role of intersectin in the mechanism of caveolae fission in endo

61 phological analysis of ECs overexpressing wt-intersectin indicated a wide range of morphological chan

62 We also showed that intersectin interaction with dynamin was important in re

63 We propose that Intersectin is a component of the endocytic machinery.

64 Intersectin is a member of a growing family of adaptor p

65 Dap160/Intersectin is a multidomain adaptor protein that coloca

66 n glycerol gradients, and cross-linking that intersectin is present in ECs in a membrane-associated p

67 The endocytic protein, Dap160/intersectin, is a major binding partner of Eps15, and ep

68 ed protein Dap160, homolog of the vertebrate Intersectins, is thought likely to act as a molecular sc

69 vered that the endocytic scaffolding protein intersectin (ITSN) cooperated with epidermal growth fact

70 Members of the intersectin (ITSN) family of scaffold proteins consist o

71 e endocytic and signal transduction scaffold intersectin (ITSN) increased aggregate formation by muta

72 Intersectin (ITSN) is a molecular scaffold involved in r

73 Intersectin (ITSN) is a multidomain scaffolding protein

74 Intersectin (ITSN) is a multimodular endocytic scaffold

75 ave highlighted a role for EH and SH3 domain Intersectin (Itsn) proteins in synaptic vesicle recyclin

76 Based on the interaction between Cdc42 and intersectin (ITSN), a specific Cdc42 guanine nucleotide

77 ion on a recently described adaptor protein, intersectin (ITSN), which provides a link to both the en

78 ne nucleotide exchange factors Elmo/Dock and intersectin (ITSN).

79 Intersectins (ITSNs) are multidomain adaptor proteins im

80 Full-length intersectin-L exhibited little ability to stimulate nucl

81 specific guanine nucleotide exchange factor, intersectin-L, in fibroblasts.

82 Together, these data suggest that intersectin links endocytosis with regulation of pathway

83 Our data suggest that EHSH1/intersectin may be a novel adaptor protein that couples

84 ound reduced Trio or Tiam1 but not Vav, Lbc, Intersectin, or a constitutively active Rac1 mutant-stim

85 First, intersectin overexpression activated the Elk-1 transcrip

86 Replacing Intersectin's regulatory domains with the BH3 peptide/Bc

87 lated by orthoIntersectin, but not wild-type Intersectin, showing that the designed interaction can t

88 nd recruited the scaffold proteins eps15 and intersectin, which in turn engaged the adaptor complex A

89 gy to the guanine nucleotide exchange factor Intersectin, which is a selective activator of the GTPas

90 rther, the structural determinants unique to intersectin, which permit selective recognition and conc

91 ver, Cdc42(Y32F) is exclusively activated by intersectin, while virtually unresponsive to other Cdc42

92 antially increases the affinity of Cdc42 for intersectin, yet severely cripples interaction with Dbs,

93 e embryo cDNA library with the EH domains of Intersectin yielded clones for the Rev-associated bindin