ライフサイエンスコーパス: intrinsic fluorescence

1 or NAD+ (detected via changes in the protein intrinsic fluorescence).

2 ged), to study the possible changes of their intrinsic fluorescence.

3 was resolved by stopped-flow measurements of intrinsic fluorescence.

4 es being detected that cannot be resolved by intrinsic fluorescence.

5 s (K(d) = 11-12 microm) and changes in FV(a) intrinsic fluorescence.

6 s C by following a decrease in the protein's intrinsic fluorescence.

7 ide at this site would result in a change in intrinsic fluorescence.

8 nctional mutants was also investigated using intrinsic fluorescence.

9 dent denaturation and by iodide quenching of intrinsic fluorescence.

10 s were replaced by Phe residues, eliminating intrinsic fluorescence.

11 ha subunit conformational change detected by intrinsic fluorescence.

12 red mammalian-cell-expressed TIMP-(1-127) by intrinsic fluorescence.

13 uman plasma were characterized by changes in intrinsic fluorescence.

14 ent with the observed spectral properties of intrinsic fluorescence.

15 distinctive circular dichroism features, and intrinsic fluorescence.

16 ld be easily detected in gels based on their intrinsic fluorescence.

17 canning calorimetry, circular dichroism, and intrinsic fluorescence.

18 scanning calorimetry, circular dichroism, or intrinsic fluorescence.

19 by large changes in Stokes radius (11 A) and intrinsic fluorescence (2-fold).

20 y and cross-linking activity, but had little intrinsic fluorescence (370/440 nm).

21 po-forms of CP by far-UV circular dichroism, intrinsic fluorescence, 8-anilinonaphthalene-1-sulfonic

22 R163K exhibited higher intrinsic fluorescence, a more negative molar ellipticit

23 Measurement of changes in intrinsic fluorescence allowed determination of a K(d) f

24 this report, we show that extraction of the intrinsic fluorescence allows us: (a) to determine the f

25 Such studies can be complicated by the intrinsic fluorescence and absorbance properties of CheA

26 esponsible for the majority of the protein's intrinsic fluorescence and all of the quenching that acc

27 ange characterized by a 2-fold change in the intrinsic fluorescence and an 11 A change in the Stokes

28 indicated by the substantial enhancement in intrinsic fluorescence and blue shift of fluorescent max

29 Temperature effects on intrinsic fluorescence and catalytic activity of hTS and

30 the stability of the R subunit was probed by intrinsic fluorescence and circular dichroism (CD) in th

31 major globular protein in kidney beans using intrinsic fluorescence and circular dichroism (CD).

32 ing and end-point structures as indicated by intrinsic fluorescence and circular dichroism, respectiv

33 Using measurements of protein intrinsic fluorescence and circular dichroism, we demons

34 mately 43 degrees C, monitored by changes in intrinsic fluorescence and circular dichroism.

35 The intrinsic fluorescence and difference absorption studies

36 Interestingly, spectral probes, such as intrinsic fluorescence and far-UV CD spectroscopy did no

37 oligomerization as determined by changes in intrinsic fluorescence and fluorescence resonance energy

38 nformational changes evidenced by changes in intrinsic fluorescence and in CD spectra and changes in

39 Rapid decreases in protein intrinsic fluorescence and increases in TNP-ATP fluoresc

40 Intrinsic fluorescence and iodide quenching in the prese

41 studied by fluorescence quenching of protein intrinsic fluorescence and nephelometry.

42 ctured, having a similar circular dichroism, intrinsic fluorescence and NMR spectrum as the protein d

43 ce anisotropy of rhodamine-labeled profilin, intrinsic fluorescence and nucleotide exchange, give the

44 matic residues (near-UV circular dichroism), intrinsic fluorescence and peptide binding activity are

45 The potential of intrinsic fluorescence and principal component analysis

46 mer formation, as demonstrated by changes in intrinsic fluorescence and quantitative native gel elect

47 t-index lens assembly for two-photon excited intrinsic fluorescence and second-harmonic generation im

48 vivo mouse tissue through the collection of intrinsic fluorescence and second-harmonic signal withou

49 Importantly, intrinsic fluorescence and sedimentation velocity analys

50 actin (W79) was generated that showed small intrinsic fluorescence and should be useful for studies

51 d by relative changes in iodide quenching of intrinsic fluorescence, and A-loop conformation was rank

52 Circular dichroism, intrinsic fluorescence, and differential scanning calori

53 nitoring changes in its absorption spectrum, intrinsic fluorescence, and fluorescence of the Nile red

54 were observed by way of circular dichroism, intrinsic fluorescence, and Forster resonance energy tra

55 ion, we investigated plastic hydrophobicity, intrinsic fluorescence, and oligo attachment efficiency.

56 electrophoresis, nuclear magnetic resonance, intrinsic fluorescence, and proteolysis assays of wild-t

57 ere monitored by the changes in the receptor intrinsic fluorescence, and the data are consistent with

58 Return of enzymatic activity, intrinsic fluorescence, and the S1 pocket appeared to oc

59 th a 25% maximal glucose-related decrease in intrinsic fluorescence, and the saturation point was inc

60 ongly blue-shifted emission spectrum, higher intrinsic fluorescence, and weaker energy transfer from

61 ion experiments measuring lambda(max) of the intrinsic fluorescence as a function of guanidine hydroc

62 An intrinsic fluorescence assay shows that TH-2/3 also bind

63 Size exclusion chromatography and a simple intrinsic fluorescence assay were used to determine that

64 Furthermore, intrinsic fluorescence assays indicated that these metal

65 heme for mutant HIV-1 RT heterodimers, using intrinsic fluorescence assays.

66 In this method we use intrinsic fluorescence biomarkers and the phasor approac

67 rescence anisotropy of dansyl-labeled GroES, intrinsic fluorescence, bis-ANS binding, sedimentation v

68 dysplastic and nondysplastic BE in terms of intrinsic fluorescence, bulk scattering properties, and

69 ing near-UV circular dichroism, quenching of intrinsic fluorescence by acrylamide and interactions wi

70 Quenching of the intrinsic fluorescence by DNA and external dynamic quenc

71 ite-specific, ATP-dependent quenching of DNR intrinsic fluorescence by spin labels.

72 further elucidate the molecular basis of the intrinsic fluorescence change (and the source of the slo

73 , nucleotide analogue trapping kinetics, and intrinsic fluorescence changes accompanying nucleotide b

74 In this paper, we demonstrate that intrinsic fluorescence changes can be used to monitor th

75 To examine the structural basis of the intrinsic fluorescence changes that occur during the MgA

76 GTP gamma S-stimulated intrinsic fluorescence changes were completely abolished

77 Substrate-dependent intrinsic fluorescence changes were monitored in transpo

78 (2+) to apoFosA alters the UV absorption and intrinsic fluorescence characteristics of the protein su

79 on of HK97 in vitro by monitoring changes in intrinsic fluorescence, circular dichroism (CD), and SAX

80 Using intrinsic fluorescence, circular dichroism, and size exc

81 mutants were generated to permit the use of intrinsic fluorescence, circular dichroism, and ultravio

82 g tissue using two- and three-photon-excited intrinsic fluorescence combined with second harmonic gen

83 Intrinsic fluorescence combined with UV resonance Raman

84 An unprecedented class of macrocycles with intrinsic fluorescence consisting of phenolic trimers an

85 the assembly dependence of the site-specific intrinsic fluorescence data suggests that the CHC is par

86 ording to hydrogen exchange and stopped-flow intrinsic fluorescence data, unfolding of mAAT appears t

87 The association of activity loss with an intrinsic fluorescence decrease and loss of the S1-pocke

88 Conversely, the intrinsic fluorescence decrease observed at higher LM pe

89 , induced a blue shift in the lambda(max) of intrinsic fluorescence derived from residues in the cent

90 gnals of aptamer binding to urea in terms of intrinsic fluorescence differences and color changes sim

91 Intrinsic fluorescence due to tryptophan of the mutant p

92 Circular dichroism (CD) and intrinsic fluorescence emission (IFE) spectra of active

93 s dependently on the ionic strength, and the intrinsic fluorescence emission maxima of its single try

94 The intrinsic fluorescence emission of each single-Trp mutan

95 Differences in intrinsic fluorescence emission spectra were observed du

96 The intrinsic fluorescence enhancement observed upon binding

97 n nucleotide binding, demonstrating that the intrinsic fluorescence enhancement of smooth muscle myos

98 k, we applied thioflavin T binding, tyrosine intrinsic fluorescence, fluorescence anisotropy measurem

99 Intrinsic fluorescence, FRET, and Fourier transform infr

100 ng using fluorescently labeled protein A and intrinsic fluorescence from fluorescein.

101 We report the enhanced intrinsic fluorescence from several proteins in proximit

102 affinity and specificity) with those of GFP (intrinsic fluorescence, high stability, expression and s

103 reactivity concomitant with disappearance of intrinsic fluorescence (IC(50) approximately 20 microM).

104 ryptase were investigated by analysis of (i) intrinsic fluorescence, (ii) inhibitor binding, and (iii

105 Intrinsic fluorescence in proteins is dominated by the t

106 ntributor to nucleotide-dependent changes of intrinsic fluorescence in smooth muscle myosin.

107 beled actin fluorescence or by a decrease in intrinsic fluorescence in the absence of pyrene-labeled

108 With their intrinsic fluorescence in the NIR2 regime and lack of ph

109 Candidates for the observed quench of intrinsic fluorescence in W512 KO-MDE include W29 and W3

110 able free amino groups, decreased tryptophan intrinsic fluorescence, increased molecular mass and Ric

111 ndings are as follows: 1) differences in the intrinsic fluorescence indicate that the Trp microenviro

112 ifferent short-chain phospholipids on enzyme intrinsic fluorescence indicates that anionic phospholip

113 M on polyacrylamide gels, differences in the intrinsic fluorescence intensity and solvent accessibili

114 In addition, the intrinsic fluorescence intensity of C403S is enhanced 3-

115 esterol/POPC large unilamellar vesicles, the intrinsic fluorescence intensity of sPLA2 shows an alter

116 Initial monitoring of changes in the intrinsic fluorescence intensity of vaults showed a 60%

117 ibrium unfolding transition was monitored by intrinsic fluorescence intensity, fluorescence anisotrop

118 unfolding can be monitored directly, because intrinsic fluorescence is quenched by denaturation.

119 nts with Trp at positions 15, 27 and 61, the intrinsic fluorescence is significantly quenched in the

120 Heparin affinity chromatography and intrinsic fluorescence kinetic analyses demonstrated tha

121 We have developed a label-free intrinsic fluorescence lifetime assay that uniquely can

122 red-shift in emission and an increase in the intrinsic fluorescence lifetime for the emitting state i

123 Intrinsic fluorescence, limited proteolysis, and molecul

124 This is the first demonstration that intrinsic fluorescence may be used to monitor calcium bi

125 (P = 1.9 x 10(-9)), was associated with skin intrinsic fluorescence measured by SCOUT DS (excitation

126 cellar or micellar state was investigated by intrinsic fluorescence measurement and dynamic light sca

127 Rather, confocal microscopy, SDS-PAGE, and intrinsic fluorescence measurements indicated tight bind

128 Intrinsic fluorescence measurements of binding by the pu

129 Circular dichroism and intrinsic fluorescence measurements revealed significant

130 Intrinsic fluorescence measurements revealed that oligom

131 Far UV CD and intrinsic fluorescence measurements show that this compa

132 Intrinsic fluorescence measurements showed that PT-(1-46

133 Intrinsic fluorescence measurements suggest the dissocia

134 Intrinsic fluorescence measurements suggested that Zn(2+

135 Intrinsic fluorescence measurements were performed to me

136 ecific GHS-R1a conformations, as assessed by intrinsic fluorescence measurements.

137 The importance of proton transfer on the intrinsic fluorescence observed in protein fibrils is si

138 ects of loop sequence and loop length on the intrinsic fluorescence of 13 DNA GQSs.

139 The intrinsic fluorescence of 3-OST-1 was increased in the p

140 est or, in selected instances, the change in intrinsic fluorescence of a DNA binding agent itself and

141 The intrinsic fluorescence of a single tryptophan (Trp-698)

142 In common fluorogenic probes, the intrinsic fluorescence of a small-molecule fluorophore i

143 Intrinsic fluorescence of a tryptophan at residue 698 (T

144 Here, we use intrinsic fluorescence of a tryptophan located at the di

145 sing two assays, including one that uses the intrinsic fluorescence of a tryptophan residue, we have

146 Using the intrinsic fluorescence of all-trans-retinyl esters, noni

147 The intrinsic fluorescence of alpha-tocopherol and its effec

148 The intrinsic fluorescence of alphaBF61N was marginally lowe

149 Using the intrinsic fluorescence of anthralin, rapid accumulation

150 rate that the increases and decreases in the intrinsic fluorescence of antithrombin during heparin bi

151 Intrinsic fluorescence of ApoL1 supported the presence o

152 er common peroxidases, (2) monitoring of the intrinsic fluorescence of dityrosine to determine optimu

153 y(G) stimulated proteolysis and quenched the intrinsic fluorescence of EF-3.

154 Furthermore, the intrinsic fluorescence of fluorobodies correlates with b

155 Glomerular imaging relied on intrinsic fluorescence of GFP or DsRed, or on whole-moun

156 The intrinsic fluorescence of GQSs may be useful for nucleic

157 The intrinsic fluorescence of hexokinase in solution (excita

158 Specifically, by analyzing intrinsic fluorescence of human epithelial tissue as it

159 ine sensing which is based on monitoring the intrinsic fluorescence of in situ synthesized polydopami

160 l as for the determination of Kd's using the intrinsic fluorescence of LT.

161 Using two-photon microscopy and the intrinsic fluorescence of N-retinylamides, we showed tha

162 states by fluorescence quenching analysis of intrinsic fluorescence of native tryptophan (Trp) residu

163 etry and environment of its chromophore, the intrinsic fluorescence of OCP reveals changes in its ter

164 by the ligand urate, which also quenches the intrinsic fluorescence of PecS, indicating a direct inte

165 Two-photon excitation is used to probe the intrinsic fluorescence of peptide fragments through "dee

166 orms a ground-state complex by quenching the intrinsic fluorescence of PPO.

167 eagents, both the enzymatic activity and the intrinsic fluorescence of PR-DeltaPol are linearly depen

168 We used changes in the intrinsic fluorescence of profilin, CD spectroscopy, and

169 The intrinsic fluorescence of smooth muscle myosin is sensit

170 L-1 was studied by monitoring changes in the intrinsic fluorescence of sP-selectin upon binding to sP

171 l biophysical analysis demonstrated that the intrinsic fluorescence of SP600125 changed markedly upon

172 The intrinsic fluorescence of Ssa1p's only tryptophan residu

173 altered nucleotide-dependent changes in the intrinsic fluorescence of Ssa1p.

174 ed video-rate imaging of tumors based on the intrinsic fluorescence of SWNTs in the second near-infra

175 sedimentation equilibrium and velocity, and intrinsic fluorescence of the 2 Trp residues.

176 Basically, the intrinsic fluorescence of the amino acid tryptophan is e

177 sion difference is due to differences in the intrinsic fluorescence of the bound fluorophores rather

178 The intrinsic fluorescence of the catalytic portion of the c

179 is was carried out to ascertain the relative intrinsic fluorescence of the enzyme intermediates.

180 The intrinsic fluorescence of the intermediate suggested tha

181 Upon binding of Ca(2+), the intrinsic fluorescence of the mutant decreases approxima

182 x formation is accompanied by an increase in intrinsic fluorescence of the primers, allowing simple a

183 This used the intrinsic fluorescence of the protein to monitor the che

184 milar changes in the vibrational spectra and intrinsic fluorescence of the protein, which indicates t

185 te and is accompanied by a blue shift in the intrinsic fluorescence of the protein.

186 The intrinsic fluorescence of the six tyrosines located with

187 By utilizing the intrinsic fluorescence of the tryptophan (Trp) and tyros

188 The intrinsic fluorescence of the tryptophans could be used

189 termined by titrations of the alterations in intrinsic fluorescence of the variant kringles with the

190 ound; and (c) Trp222 contributes most to the intrinsic fluorescence of the wt-RI-subunit, while Trp18

191 Mg2+ altered the tryptophan intrinsic fluorescence of this polypeptide whereas Ba2+,

192 e formation in this part of the protein; the intrinsic fluorescence of this tryptophan was found to d

193 The intrinsic fluorescence of Tp38 was essentially unaltered

194 Thus the intrinsic fluorescence of Trp-11 provides a useful probe

195 However, changes in the intrinsic fluorescence of truncated and Trp16 mutants up

196 n constant estimated from the changes in the intrinsic fluorescence of tubulin was found to be consis

197 Spectroscopy examining the intrinsic fluorescence of tyrosine and tryptophan residu

198 o residues of tryptophan, which dominate the intrinsic fluorescence of unlabeled CF1.

199 Whereas the intrinsic fluorescence of W36-MDE is only slightly sensi

200 In this study, we used intrinsic fluorescence of wild type Abeta-(1-40), fluore

201 ese tryptophans was found to account for the intrinsic fluorescence of wild-type actin, indicating th

202 lcium titrations monitored by changes in the intrinsic fluorescence of Y138 in site IV showed that th

203 s study, we investigated measurements of the intrinsic fluorescence of yeast hexokinase as an assay f

204 glutenins and gliadins was analyzed through intrinsic fluorescence parameters, phase diagram method,

205 In addition, the intrinsic fluorescence polarization increases from 0.327

206 ptophan mutation at position 39 to create an intrinsic fluorescence probe and allow additional charac

207 on dynamics, using 2-aminopurine (Ap) as the intrinsic fluorescence probe and with femtosecond resolu

208 ng, we developed the ds-NIF (nucleoside with intrinsic fluorescence)-probe methodology for detection

209 turbation assay in which a change in the MAb intrinsic fluorescence produced by ligand binding was us

210 The proteins' similar intrinsic fluorescence properties and corresponding quen

211 nvironment, we have studied the steady-state intrinsic fluorescence properties of the purine and pyri

212 predicted binding to complement C3d and with intrinsic fluorescence properties to enable detection.

213 nm, and after purification, the QDs exhibit intrinsic fluorescence quantum yield efficiencies of 15

214 Immunological and intrinsic fluorescence quenching assays and the molecula

215 of 0.24 microM for the Efb-C3d binding using intrinsic fluorescence quenching assays.

216 Isothermal calorimetry and intrinsic fluorescence quenching studies show that oxida

217 nderwent normal, two-stage calcium-dependent intrinsic fluorescence quenching, indicating that a fold

218 g SDS-polyacrylamide gel electrophoresis and intrinsic fluorescence quenching.

219 Intrinsic fluorescence, reflectance, and light-scatterin

220 tryptophans at various locations to serve as intrinsic fluorescence reporters, i.e. as topological pr

221 Whereas the collective, intrinsic fluorescence response of E. coli CPS is largel

222 Near UV CD and intrinsic fluorescence revealed that the tryptophan resi

223 Intrinsic fluorescence showed that wild-type rFV(a2)-C2

224 Previous work, in which the increase in the intrinsic fluorescence signal was used to monitor iron r

225 ignature that was readily separated from the intrinsic fluorescence signature of tumor cells.

226 Analysis of intrinsic fluorescence spectra demonstrates that uric ac

227 Intrinsic fluorescence spectra for both proteins were al

228 tion coefficient mapping has been applied to intrinsic fluorescence spectra of colonic tissue for the

229 Then, we extract the intrinsic fluorescence spectra of sites from 35 patients

230 e emission peak, and causes quenching of the intrinsic fluorescence spectra of the proteins.

231 Intrinsic fluorescence spectra suggest that the associat

232 cularly, phospholipid/saposin C complexes by intrinsic fluorescence spectral shifts, fluorescence que

233 upon calcium binding, which was confirmed by intrinsic fluorescence spectroscopy and nondenaturing ge

234 Intrinsic fluorescence spectroscopy of purified proteins

235 ed from isothermal titration calorimetry and intrinsic fluorescence spectroscopy suggests that YKL-39

236 ptical cancer detection system that combines intrinsic fluorescence spectroscopy, diffuse reflectance

237 The highly sensitive method of intrinsic fluorescence spectroscopy, predicated on the m

238 was monitored by both circular dichroism and intrinsic fluorescence spectroscopy.

239 Analysis of the intrinsic fluorescence, Stokes radius, thermal stability

240 Intrinsic fluorescence studies of the two mutants reveal

241 Intrinsic fluorescence studies were conducted to determi

242 d properties such as near- and far-UV CD and intrinsic fluorescence studies.

243 -UV circular dichroism spectrum and a higher intrinsic fluorescence than the heterodimer, demonstrati

244 We have characterized the changes in intrinsic fluorescence that the insulin receptor undergo

245 by monitoring the quenching of the protein's intrinsic fluorescence; the tartronate concentration dep

246 ith pyrene-labeled yeast actin, but not with intrinsic fluorescence, there is an overshoot in the flu

247 With the aid of two PNA analogues having intrinsic fluorescence, thiazole orange (TO)-PNA and [bi

248 An intrinsic fluorescence titration of various alpha-LA for

249 properties of rNK1/K1 were investigated via intrinsic fluorescence titration with kringle-specific o

250 spectroscopy, sedimentation equilibrium, and intrinsic fluorescence to monitor the reversible conform

251 Using a combination of intrinsic fluorescence to report ATP-induced rearrangeme

252 sines to the C-terminal domain of TBP allows intrinsic fluorescence to separately report on the struc

253 rmeability, direct membrane association, and intrinsic fluorescence to study the activities of purifi

254 We have used intrinsic fluorescence to test the hypothesis that phosp

255 domain of p53 (p53C) using light scattering, intrinsic fluorescence, transmission electron microscopy

256 n HCT116 cells was measured using the drug's intrinsic fluorescence under conditions that alter pH(i)

257 al pH of 5.6, measurement of the increase in intrinsic fluorescence upon iron release from the recomb

258 Changes in intrinsic fluorescence upon nucleotide and inositide bin

259 phans except W512 (W512 KO-MDE) decreases in intrinsic fluorescence upon nucleotide binding, demonstr

260 and 700 MPa, where a significant decrease in intrinsic fluorescence was also observed.

261 ells for intracellular injection using their intrinsic fluorescence was developed.

262 e treatment whereas a noticeable decrease in intrinsic fluorescence was evidenced up to 600 MPa and t

263 A complex, time-dependent change in enzyme intrinsic fluorescence was observed upon exposure to glu

264 targeting and design of assembly inhibitors, intrinsic fluorescence was used to probe assembly-depend

265 g of wild-type RIalpha (wt-RI), monitored by intrinsic fluorescence, was described previously.

266 tains no tryptophan residues and exhibits no intrinsic fluorescence, was harvested from CHO cells cot

267 Activity and intrinsic fluorescence were used to measure the stabiliz

268 t protein structural changes, as measured by intrinsic fluorescence, were much slower (t1/2 = 16 min)

269 nding; (b) a temperature-induced increase in intrinsic fluorescence, which reflects formation of a tr

270 yll a molecules, which confer on them a high intrinsic fluorescence yield.