ライフサイエンスコーパス: inverse PCR

1 genome walking methods such as the prominent inverse PCR.

2 d for randomly integrated viral DNA by using inverse PCR.

3 nce were obtained in Medicago truncatula via inverse PCR.

4 e 5' end of the partial ORF was cloned using inverse PCR.

5 ed upon these peptide sequences, followed by inverse PCR.

6 onuclease restriction sequence engineered by inverse PCR.

7 ntica chromosome that we identified by using inverse PCR.

8 stearothermophilus genomic DNA using PCR and inverse PCR.

9 uences of the Tn10 inserts were amplified by inverse PCR.

10 f the NlaIII endonuclease gene was cloned by inverse PCR.

11 tilis, was cloned in two steps by normal and inverse PCR.

12 ertion points were refractory to analysis by inverse-PCR.

13 By using inverse PCR, 28 unique viral integration sites were iden

14 ated with degenerate PCR primers followed by inverse PCR amplification.

15 By positioning the sequences of inverse PCR-amplified, virus-host junction fragments wit

16 Inverse PCR analysis of these clones revealed that the r

17 ith the flanking genomic DNA is harvested by inverse PCR and its genomic location is determined by hy

18 ping together with molecular methods such as inverse PCR and quantitative PCR have allowed more preci

19 nds of the inserted sequences were cloned by inverse PCR and revealed an intracisternal A-particle (I

20 Subsequent inverse PCR and sequence analyses identified the transpo

21 Inverse PCR and sequence analysis revealed that the Tn 1

22 n of DEA1, the promoter region was cloned by inverse PCR and was found to contain putative stress-, s

23 ed from the T-47D breast cancer cell line by inverse-PCR and cloning.

24 ysis of results from genomic Southern blots, inverse PCR, and sequencing revealed that the lcf gene i

25 The 5' region was obtained by inverse PCR, and the complete gene sequence was determin

26 g Escherichia coli strains were amplified by inverse PCR, and the nucleotide sequences of the junctio

27 ric epsilon-cyclases constructed by using an inverse PCR approach.

28 ing the Tn5 flanking sequences (amplified by inverse PCR) as a probe, an S. meliloti genomic library

29 Here, we describe inverse PCR-based amplified restriction fragment length

30 Inverse PCR combined with genomic analyses identified P

31 II inhibitors, we developed a long-distance inverse PCR DNA-based assay for chimeric Mll fusions in

32 atocellular carcinoma (HCC), was analyzed by inverse PCR for randomly integrated HBV DNA as a marker

33 estriction fragment length polymorphisms and inverse PCR fragments generated from the PHYB gene of wi

34 Inverse PCR from the retroviral long terminal repeat int

35 s sequence has been compared with that of an inverse PCR-generated der(11) junction fragment obtained

36 via coligation of insert termini followed by inverse PCR generates a jumping library for paired-end s

37 Here we describe an inverse PCR (IPCR) method for proviral tagging that make

38 hermal asymmetric interlaced PCR (TAIL-PCR), inverse PCR (IPCR), or partial library construction.

39 m genomic DNA by use of the direct (PCR) and inverse PCR (IPCR).

40 ments with long-distance PCR (long-distance, inverse PCR [LDI-PCR]).

41 and further extension of the sequence using inverse PCR led to the cloning of the entire leukotoxin

42 ic TEL-AML1 fusion gene with a long-distance inverse PCR method.

43 Inverse PCR methods were used to obtain the complete seq

44 se protection assays, and independently with inverse PCR of 5' RACE clones, common mRNA initiation si

45 A1) and H2A2 (HTA2) were then isolated using inverse PCR on circularized genomic DNA fragments.

46 abase and retrieving of full-length cDNA via inverse PCR on subdivided primary cDNA library pools.

47 This reveals that the inverse PCR product has sustained a deletion between two

48 nctions were determined by DNA sequencing of inverse PCR products.

49 the tagged gene is determined by sequencing inverse-PCR products derived from genomic DNA.

50 phism (iFLP), a new technology that combines inverse PCR, RFLP, and denaturing high-performance liqui

51 Inverse PCR strategy for integration site analysis demon

52 A long-distance inverse PCR strategy was used to amplify TEL-AML1 genomi

53 By use of inverse PCR technology and the construction of several a

54 ing the insertion site junctions isolated by inverse PCR that identified a characteristic piggyBac TT

55 at are repressive for transcription, we used inverse PCR to characterize the HIV-1 integration sites

56 We used inverse PCR to clone and analyze 212 retroviral integrat

57 We successfully used inverse PCR to clone and sequence the flanking DNA for a

58 was extracted from the adjacent section for inverse PCR to detect integrated HBV DNA.

59 Second, we used long-template inverse PCR to focus on gene rearrangements at the MLL l

60 In the first, students learn to perform inverse PCR to identify the genomic location of the GAL4

61 APDH) processed pseudogene was identified by inverse PCR using oligonucleotide primers specific for t

62 Two rounds of inverse-PCR using genomic DNA permitted the isolation of

63 By use of inverse PCR, vanD, vanHD, and two partial flanking open-

64 Inverse PCR was used to derive 2345 bp of upstream Ltp6

65 cent In Situ Hybridization and Long Distance Inverse-PCR we disclosed that these transcripts result f

66 Using high throughput inverse PCR, we cloned and analyzed the sequences of 884

67 Using inverse PCR, we determined that the rat genome contains